EGFLAM (NM_152403) Human Tagged ORF Clone

CAT#: RG220450

EGFLAM (GFP-tagged) - Human EGF-like, fibronectin type III and laminin G domains (EGFLAM), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EGFLAM
• Synonyms: AGRINL; AGRNL; PIKA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220450 representing NM_152403
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTAATCCGAGGCGTCTTGCTCCGGCTCCTGCTCCTGGCTTCCAGCCTCGGACCCGGCGCGGTGT
  CGCTCCGAGCGGCCATCCGAAAACCAGGCAAGGTAGGGCCTCCTCTTGACATCAAGCTGGGCGCATTGAA
  CTGTACGGCTTTCAGCATCCAGTGGAAAATGCCAAGGCATCCTGGAAGTCCCATCCTTGGGTACACTGTC
  TTTTACTCTGAGGTTGGCGCAGATAAATCCCTGCAGGAGCAGTTGCACAGCGTGCCTCTCAGCCGGGACA
  TCCCGACCACGGAGGAAGTGATTGGAGATTTGAAACCAGGCACTGAATATCGTGTGAGCATAGCAGCTTA
  CAGCCAGGCTGGCAAAGGGCGGCTGAGCTCTCCTCGGCATGTCACCACTTTGTCCCAAGATTCCTGCCTG
  CCTCCTGCAGCTCCCCAGCAGCCACATGTCATTGTGGTTTCGGATTCTGAGGTGGCCCTGTCTTGGAAAC
  CTGGAGCGAGTGAAGGAAGCGCCCCTATTCAGTACTATTCTGTGGAATTCATCAGGCCAGATTTCGACAA
  GAAGTGGACCTCAATCCATGAGCGGATCCAGATGGACTCCATGGTTATCAAGGGCCTCGATCCAGATACC
  AACTACCAGTTTGCCGTGAGGGCAATGAATTCCCATGGCCCCAGCCCCCGCAGCTGGCCCAGTGACATCA
  TCCGGACCCTCTGCCCTGAGGAGGCGGGAAGTGGCCGCTATGGACCCCGTTATATCACCGACATGGGAGC
  TGGTGAGGATGATGAAGGATTTGAAGACGACTTAGATTTGGATATTTCCTTTGAGGAGGTTAAACCACTT
  CCTGCTACCAAAGGAGGGAATAAGAAATTTTTGGTGGAAAGCAAGAAGATGTCTATATCTAACCCAAAGA
  CCATTTCTAGGCTCATCCCCCCTACCTCAGCATCTCTCCCTGTGACCACGGTGGCTCCCCAGCCCATTCC
  CATACAGAGAAAGGGGAAGAATGGTGTGGCCATAATGTCAAGGCTCTTTGACATGCCTTGTGATGAAACT
  CTCTGCTCTGCTGACAGCTTCTGTGTCAATGACTACACCTGGGGGGGCTCGCGATGCCAGTGCACCCTGG
  GCAAAGGTGGTGAGAGCTGCTCAGAAGATATTGTTATCCAGTATCCTCAGTTCTTTGGCCACTCCTATGT
  AACGTTTGAACCTCTGAAGAATTCTTATCAGGCATTTCAAATTACTCTTGAATTTAGGGCGGAGGCAGAG
  GATGGCTTACTGCTCTACTGTGGGGAGAACGAACACGGGAGGGGGGATTTCATGTCCCTGGCTATCATCC
  GACGCTCCCTGCAGTTCAGGTTTAATTGTGGAACTGGGGTTGCCATCATCGTAAGTGAGACCAAAATCAA
  ACTAGGGGGTTGGCACACGGTTATGCTCTACAGAGATGGGCTGAACGGGCTGCTGCAGCTGAACAATGGC
  ACCCCAGTGACAGGCCAGTCTCAGGGCCAATACAGTAAAATTACTTTCCGGACACCTCTCTATCTTGGTG
  GCGCTCCCAGCGCTTACTGGTTGGTTAGAGCAACAGGGACAAACCGAGGCTTTCAAGGCTGTGTGCAGTC
  GCTCGCTGTGAATGGGAGGAGAATTGACATGAGGCCCTGGCCCCTGGGAAAAGCACTCAGTGGGGCTGAT
  GTGGGGGAATGCAGCAGTGGAATCTGTGATGAGGCCTCGTGCATCCATGGTGGCACCTGCACAGCAATCA
  AAGCCGACTCCTACATTTGCCTCTGTCCCCTTGGGTTTAAAGGTCGACACTGTGAAGATGCTTTCACCTT
  GACCATTCCTCAGTTCAGAGAGTCTCTGAGATCTTACGCTGCAACTCCCTGGCCACTGGAGCCCCAGCAT
  TACCTTTCCTTCATGGAATTTGAGATCACATTTCGGCCAGACTCAGGAGATGGTGTCCTCCTGTACAGCT
  ATGACACAGGCAGCAAAGACTTCCTGTCCATCAACTTGGCAGGGGGCCACGTGGAGTTCCGCTTTGACTG
  TGGCTCTGGGACCGGTGTCCTCAGGAGTGAAGATCCCCTCACCCTGGGCAACTGGCACGAGCTTCGTGTA
  TCTCGCACAGCAAAGAATGGAATCTTACAGGTGGATAAGCAGAAGATAGTGGAGGGAATGGCAGAGGGAG
  GCTTCACACAGATTAAGTGCAACACAGACATTTTCATTGGCGGAGTCCCCAATTATGATGATGTGAAGAA
  GAACTCGGGTGTCCTGAAGCCTTTCAGCGGGAGCATCCAGAAGATCATCCTGAATGACCGAACCATCCAT
  GTGAAGCATGACTTCACCTCCGGAGTGAATGTGGAGAATGCGGCCCACCCCTGTGTGAGAGCCCCTTGTG
  CCCATGGGGGCAGCTGCCGGCCCAGGAAGGAGGGCTATGACTGTGACTGCCCCTTGGGCTTTGAGGGGCT
  TCACTGCCAGAAAGCGATCATAGAAGCCATTGAGATCCCGCAGTTTATCGGCCGCAGTTACCTGACGTAT
  GACAACCCAGATATCTTGAAGAGGGTGTCAGGATCAAGATCAAATGTGTTCATGAGGTTTAAAACAACTG
  CCAAGGATGGCCTTTTGCTGTGGAGGGGAGACAGCCCCATGAGACCCAACAGCGACTTCATTTCCTTGGG
  CCTTCGGGATGGAGCCCTCGTGTTCAGCTATAACCTGGGCAGTGGTGTGGCATCCATCATGGTGAATGGC
  TCCTTCAACGATGGTCGGTGGCACCGAGTTAAGGCCGTTAGGGATGGCCAGTCAGGAAAGATAACCGTGG
  ATGACTATGGAGCCAGAACAGGCAAATCCCCAGGCATGATGCGGCAGCTTAACATCAATGGAGCTCTGTA
  TGTGGGTGGAATGAAGGAAATTGCTCTGCACACTAACAGGCAATATATGAGAGGGCTCGTGGGCTGTATC
  TCTCACTTCACCCTGTCCACCGATTACCACATTTCCCTCGTGGAAGATGCCGTGGATGGGAAAAACATCA
  ACACTTGTGGAGCCAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220450 representing NM_152403
  Red=Cloning site Green=Tags(s)
  
  MDLIRGVLLRLLLLASSLGPGAVSLRAAIRKPGKVGPPLDIKLGALNCTAFSIQWKMPRHPGSPILGYTV
  FYSEVGADKSLQEQLHSVPLSRDIPTTEEVIGDLKPGTEYRVSIAAYSQAGKGRLSSPRHVTTLSQDSCL
  PPAAPQQPHVIVVSDSEVALSWKPGASEGSAPIQYYSVEFIRPDFDKKWTSIHERIQMDSMVIKGLDPDT
  NYQFAVRAMNSHGPSPRSWPSDIIRTLCPEEAGSGRYGPRYITDMGAGEDDEGFEDDLDLDISFEEVKPL
  PATKGGNKKFLVESKKMSISNPKTISRLIPPTSASLPVTTVAPQPIPIQRKGKNGVAIMSRLFDMPCDET
  LCSADSFCVNDYTWGGSRCQCTLGKGGESCSEDIVIQYPQFFGHSYVTFEPLKNSYQAFQITLEFRAEAE
  DGLLLYCGENEHGRGDFMSLAIIRRSLQFRFNCGTGVAIIVSETKIKLGGWHTVMLYRDGLNGLLQLNNG
  TPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQGCVQSLAVNGRRIDMRPWPLGKALSGAD
  VGECSSGICDEASCIHGGTCTAIKADSYICLCPLGFKGRHCEDAFTLTIPQFRESLRSYAATPWPLEPQH
  YLSFMEFEITFRPDSGDGVLLYSYDTGSKDFLSINLAGGHVEFRFDCGSGTGVLRSEDPLTLGNWHELRV
  SRTAKNGILQVDKQKIVEGMAEGGFTQIKCNTDIFIGGVPNYDDVKKNSGVLKPFSGSIQKIILNDRTIH
  VKHDFTSGVNVENAAHPCVRAPCAHGGSCRPRKEGYDCDCPLGFEGLHCQKAIIEAIEIPQFIGRSYLTY
  DNPDILKRVSGSRSNVFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNG
  SFNDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNINGALYVGGMKEIALHTNRQYMRGLVGCI
  SHFTLSTDYHISLVEDAVDGKNINTCGAK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152403
• ORF Size: 3027 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_152403.2, NP_689616.2
• RefSeq Size: 3952
• RefSeq ORF: 3030
• Locus ID: 133584
• Protein Families: Transmembrane