Thyroid Peroxidase (TPO) (NM_175722) Human Tagged ORF Clone

CAT#: RG220611

TPO (GFP-tagged) - Human thyroid peroxidase (TPO), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TPO
• Synonyms: MSA; TDH2A; TPX
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220611 representing NM_175722
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAGCGCTCGCTGTGCTGTCTGTCACGCTGGTTATGGCCTGCACAGAAGCCTTCTTCCCCTTCATCT
  CGAGAGGGAAAGAACTCCTTTGGGGAAAGCCTGAGGAGTCTCGTGTCTCTAGCGTCTTGGAGGAAAGCAA
  GCGCCTGGTGGACACCGCCATGTACGCCACGATGCAGAGAAACCTCAAGAAAAGAGGAATCCTTTCTCCA
  GCTCAGCTTCTGTCTTTTTCCAAACTTCCTGAGCCAACAAGCGGAGTGATTGCCCGAGCAGCAGAGATAA
  TGGAAACATCAATACAAGCGATGAAAAGAAAAGTCAACCTGAAAACTCAACAATCACAGCATCCAACGGA
  TGCTTTATCAGAAGATCTGCTGAGCATCATTGCAAACATGTCTGGATGTCTCCCTTACATGCTGCCCCCA
  AAATGCCCAAACACTTGCCTGGCGAACAAATACAGGCCCATCACAGGAGCTTGCAACAACAGAGACCACC
  CCAGATGGGGCGCCTCCAACACGGCCCTGGCACGATGGCTCCCTCCAGTCTATGAGGACGGCTTCAGTCA
  GCCCCGAGGCTGGAACCCCGGCTTCTTGTACAACGGGTTCCCACTGCCCCCGGTCCGGGAGGTGACAAGA
  CATGTCATTCAAGTTTCAAATGAGGTTGTCACAGATGATGACCGCTATTCTGACCTCCTGATGGCATGGG
  GACAATACATCGACCACGACATCGCGTTCACACCACAGAGCACCAGCAAAGCTGCCTTCGGGGGAGGGGC
  TGACTGCCAGATGACTTGTGAGAACCAAAACCCATGTTTTCCCATACAAATCATCACCCTGAGGGATTAC
  ATCCCCAGGATCCTGGGACCCGAGGCCTTCCAGCAGTACGTGGGTCCCTATGAAGGCTATGACTCCACCG
  CCAACCCCACTGTGTCCAACGTGTTCTCCACAGCCGCCTTCCGCTTCGGCCATGCCACGATCCACCCGCT
  GGTGAGGAGGCTGGACGCCAGCTTCCAGGAGCACCCCGACCTGCCCGGGCTGTGGCTGCACCAGGCTTTC
  TTCAGCCCATGGACATTACTCCGTGGAGGTGGTTTGGACCCACTAATACGAGGCCTTCTTGCAAGACCAG
  CCAAACTGCAGGTGCAGGATCAGCTGATGAACGAGGAGCTGACGGAAAGGCTCTTTGTGCTGTCCAATTC
  CAGCACCTTGGATCTGGCGTCCATCAACCTGCAGAGGGGCCGGGACCACGGGCTGCCAGGTTACAATGAG
  TGGAGGGAGTTCTGCGGCCTGCCTCGCCTGGAGACCCCCGCTGACCTGAGCACAGCCATCGCCAGCAGGA
  GCGTGGCCGACAAGATCCTGGACTTGTACAAGCATCCTGACAACATCGATGTCTGGCTGGGAGGCTTAGC
  TGAAAACTTCCTCCCCAGGGCTCGGACAGGGCCCCTGTTTGCCTGTCTCATTGGGAAGCAGATGAAGGCT
  CTGCGGGACGGTGACTGGTTTTGGTGGGAGAACAGCCACGTCTTCACGGATGCACAGAGGCGTGAGCTGG
  AGAAGCACTCCCTGTCTCGGGTCATCTGTGACAACACTGGCCTCACCAGGGTGCCCATGGATGCCTTCCA
  AGTCGGCAAATTCCCCGAAGACTTTGAGTCTTGTGACAGCATCACTGGCATGAACCTGGAGGCCTGGAGG
  GAAACCTTTCCTCAAGACGACAAGTGTGGCTTCCCAGAGAGCGTGGAGAATGGGGACTTTGTGCACTGTG
  AGGAGTCTGGGAGGCGCGTGCTGGTGTATTCCTGCCGGCACGGGTATGAGCTCCAAGGCCGGGAGCAGCT
  CACTTGCACCCAGGAAGGATGGGATTTCCAGCCTCCCCTCTGCAAAGATGTGAACGAGTGTGCAGACGGT
  GCCCACCCCCCCTGCCACGCCTCTGCGAGGTGCAGAAACACCAAAGGCGGCTTCCAGTGTCTCTGCGCGG
  ACCCCTACGAGTTAGGAGACGATGGGAGAACCTGCGTAGACTCCGGGAGGCTCCCTCGGGTGACTTGGAT
  CTCCATGTCGCTGGCTGCTCTGCTGATCGGAGGCTTCGCAGGTCTCACCTCGACGGTGATTTGCAGGTGG
  ACACGCACTGGCACTAAATCCACACTGCCCATCTCGGAGACAGGCGGAGGAACTCCCGAGCTGAGATGCG
  GAAAGCACCAGGCCGTAGGGACCTCACCGCAGCGGGCCGCAGCTCAGGACTCGGAGCAGGAGAGTGCTGG
  GATGGAAGGCCGGGATACTCACAGGCTGCCGAGAGCCCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220611 representing NM_175722
  Red=Cloning site Green=Tags(s)
  
  MRALAVLSVTLVMACTEAFFPFISRGKELLWGKPEESRVSSVLEESKRLVDTAMYATMQRNLKKRGILSP
  AQLLSFSKLPEPTSGVIARAAEIMETSIQAMKRKVNLKTQQSQHPTDALSEDLLSIIANMSGCLPYMLPP
  KCPNTCLANKYRPITGACNNRDHPRWGASNTALARWLPPVYEDGFSQPRGWNPGFLYNGFPLPPVREVTR
  HVIQVSNEVVTDDDRYSDLLMAWGQYIDHDIAFTPQSTSKAAFGGGADCQMTCENQNPCFPIQIITLRDY
  IPRILGPEAFQQYVGPYEGYDSTANPTVSNVFSTAAFRFGHATIHPLVRRLDASFQEHPDLPGLWLHQAF
  FSPWTLLRGGGLDPLIRGLLARPAKLQVQDQLMNEELTERLFVLSNSSTLDLASINLQRGRDHGLPGYNE
  WREFCGLPRLETPADLSTAIASRSVADKILDLYKHPDNIDVWLGGLAENFLPRARTGPLFACLIGKQMKA
  LRDGDWFWWENSHVFTDAQRRELEKHSLSRVICDNTGLTRVPMDAFQVGKFPEDFESCDSITGMNLEAWR
  ETFPQDDKCGFPESVENGDFVHCEESGRRVLVYSCRHGYELQGREQLTCTQEGWDFQPPLCKDVNECADG
  AHPPCHASARCRNTKGGFQCLCADPYELGDDGRTCVDSGRLPRVTWISMSLAALLIGGFAGLTSTVICRW
  TRTGTKSTLPISETGGGTPELRCGKHQAVGTSPQRAAAQDSEQESAGMEGRDTHRLPRAL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175722
• ORF Size: 2280 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175722.3, NP_783653.1
• RefSeq Size: 2543 bp
• RefSeq ORF: 2283 bp
• Locus ID: 7173
• Cytogenetics: 2p25.3
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway, Metabolic pathways, Tyrosine metabolism
• Gene Summary: 'This gene encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, May 2011]'