ALDH4A1 (NM_003748) Human Tagged ORF Clone

CAT#: RG220893

ALDH4A1 (GFP-tagged) - Human aldehyde dehydrogenase 4 family, member A1 (ALDH4A1), nuclear gene encoding mitochondrial protein, transcript variant P5CDhL

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ALDH4A1
• Synonyms: ALDH4; P5CD; P5CDh
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220893 representing NM_003748
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTGCCGGCGCCCGCGCTCCGCCGCGCCCTGCTGTCCCGCCCCTGGACCGGGGCCGGCCTGCGGT
  GGAAGCACACCTCCTCCCTGAAGGTGGCCAACGAGCCCGTCTTAGCCTTCACGCAGGGCAGCCCTGAGCG
  AGATGCCCTGCAAAAGGCCTTGAAGGACCTGAAGGGCCGGATGGAAGCCATCCCATGCGTGGTGGGGGAT
  GAGGAGGTGTGGACGTCGGACGTGCAGTACCAAGTGTCGCCTTTTAACCATGGACATAAGGTGGCCAAGT
  TCTGTTATGCAGACAAGAGCCTGCTCAACAAAGCCATTGAGGCTGCCCTGGCTGCCCGGAAAGAGTGGGA
  CCTGAAGCCTATTGCAGACCGGGCCCAGATCTTCCTGAAGGCGGCAGACATGCTGAGTGGGCCGCGCAGG
  GCTGAGATCCTCGCCAAGACCATGGTGGGACAGGGTAAGACCGTGATCCAAGCGGAGATTGACGCTGCAG
  CGGAACTCATCGACTTCTTCCGGTTCAATGCCAAGTATGCGGTGGAGCTGGAGGGGCAGCAGCCCATCAG
  CGTGCCCCCGAGCACCAACAGCACGGTGTACCGGGGTCTGGAGGGCTTCGTGGCGGCCATCTCGCCCTTT
  AACTTCACTGCAATCGGCGGCAACCTGGCGGGGGCACCGGCCCTGATGGGCAACGTGGTCCTATGGAAGC
  CCAGTGACACTGCCATGCTGGCCAGCTATGCTGTCTACCGCATCCTTCGGGAGGCTGGCCTGCCCCCCAA
  CATCATCCAGTTTGTGCCAGCTGATGGGCCCCTATTTGGGGACACTGTCACCAGCTCAGAGCACCTCTGT
  GGCATCAACTTCACAGGCAGTGTGCCCACCTTCAAACACCTGTGGAAGCAGGTGGCCCAGAACCTGGACC
  GGTTCCACACCTTCCCACGCCTGGCTGGAGAGTGCGGCGGAAAGAACTTCCACTTCGTGCACCGCTCGGC
  CGACGTGGAGAGCGTGGTGAGCGGGACCCTCCGCTCAGCCTTCGAGTACGGTGGCCAGAAGTGTTCCGCC
  TGCTCGCGTCTCTACGTGCCGCACTCGCTGTGGCCGCAGATCAAAGGGCGGCTGCTGGAGGAGCACAGTC
  GGATCAAAGTGGGCGACCCTGCAGAGGATTTTGGGACCTTCTTCTCTGCAGTGATTGATGCCAAGTCCTT
  TGCCCGTATCAAGAAGTGGCTGGAGCACGCGCGCTCCTCGCCCAGCCTCACCATCCTGGCTGGGGGCAAG
  TGTGATGACTCCGTGGGCTACTTTGTGGAGCCCTGCATCGTGGAGAGCAAGGACCCTCAGGAGCCCATCA
  TGAAGGAGGAGATCTTCGGGCCTGTACTGTCTGTGTACGTCTACCCGGACGACAAGTACAAGGAGACGCT
  GCAGCTGGTTGACAGCACCACCAGCTATGGCCTCACGGGGGCAGTGTTCTCCCAGGATAAGGACGTCGTG
  CAGGAGGCCACAAAGGTGCTGAGGAATGCTGCCGGCAACTTCTACATCAACGACAAGTCCACTGGCTCGA
  TAGTGGGCCAGCAGCCCTTTGGGGGGGCCCGAGCCTCTGGAACCAATGACAAGCCAGGGGGCCCACACTA
  CATCCTGCGCTGGACGTCGCCGCAGGTCATCAAGGAGACACATAAGCCCCTGGGGGACTGGAGCTACGCG
  TACATGCAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220893 representing NM_003748
  Red=Cloning site Green=Tags(s)
  
  MLLPAPALRRALLSRPWTGAGLRWKHTSSLKVANEPVLAFTQGSPERDALQKALKDLKGRMEAIPCVVGD
  EEVWTSDVQYQVSPFNHGHKVAKFCYADKSLLNKAIEAALAARKEWDLKPIADRAQIFLKAADMLSGPRR
  AEILAKTMVGQGKTVIQAEIDAAAELIDFFRFNAKYAVELEGQQPISVPPSTNSTVYRGLEGFVAAISPF
  NFTAIGGNLAGAPALMGNVVLWKPSDTAMLASYAVYRILREAGLPPNIIQFVPADGPLFGDTVTSSEHLC
  GINFTGSVPTFKHLWKQVAQNLDRFHTFPRLAGECGGKNFHFVHRSADVESVVSGTLRSAFEYGGQKCSA
  CSRLYVPHSLWPQIKGRLLEEHSRIKVGDPAEDFGTFFSAVIDAKSFARIKKWLEHARSSPSLTILAGGK
  CDDSVGYFVEPCIVESKDPQEPIMKEEIFGPVLSVYVYPDDKYKETLQLVDSTTSYGLTGAVFSQDKDVV
  QEATKVLRNAAGNFYINDKSTGSIVGQQPFGGARASGTNDKPGGPHYILRWTSPQVIKETHKPLGDWSYA
  YMQ
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003748
• ORF Size: 1689 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_003748.2, NP_003739.2
• RefSeq Size: 3160
• RefSeq ORF: 1692
• Locus ID: 8659
• Domains: aldedh
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Metabolic pathways
• Gene Summary: This protein belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2009]