PKN2 (NM_006256) Human Tagged ORF Clone

CAT#: RG220989

PKN2 (GFP-tagged) - Human protein kinase N2 (PKN2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PKN2
• Synonyms: Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220989 representing NM_006256
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCAACCCCGAACGGGGGGAGATTCTGCTCACGGAACTGCAGGGGGATTCCCGAAGTCTTCCGT
  TTTCTGAGAATGTGAGTGCTGTTCAAAAATTAGACTTTTCAGATACAATGGTGCAGCAGAAATTGGATGA
  TATCAAGGATCGAATTAAGAGAGAAATAAGGAAAGAACTGAAAATCAAAGAAGGAGCTGAAAATCTGAGG
  AAAGTCACAACAGATAAAAAAAGTTTGGCTTATGTAGACAACATTTTGAAAAAATCAAATAAAAAATTAG
  AAGAACTACATCACAAGCTGCAGGAATTAAATGCACATATTGTTGTATCAGATCCAGAAGATATTACAGA
  TTGCCCAAGGACTCCAGATACTCCAAATAATGACCCTCGTTGTTCTACTAGCAACAATAGATTGAAGGCC
  TTACAAAAACAATTGGATATAGAACTTAAAGTAAAACAAGGTGCAGAGAATATGATACAGATGTATTCAA
  ATGGATCTTCAAAGGATCGGAAACTCCATGGTACAGCTCAGCAACTGCTCCAGGACAGCAAGACAAAAAT
  AGAAGTCATACGAATGCAGATTCTTCAGGCAGTCCAGACTAATGAATTGGCTTTTGATAATGCAAAACCT
  GTGATAAGTCCTCTTGAACTTCGGATGGAAGAATTAAGGCATCATTTTAGGATAGAGTTTGCAGTAGCAG
  AAGGTGCAAAGAATGTAATGAAATTACTTGGCTCAGGAAAAGTAACAGACAGAAAAGCACTTTCAGAAGC
  TCAAGCAAGATTTAATGAATCAAGTCAGAAGTTGGACCTTTTAAAGTATTCATTAGAGCAAAGATTAAAC
  GAAGTCCCCAAGAATCATCCCAAAAGCAGGATTATTATTGAAGAACTTTCACTTGTTGCTGCATCACCAA
  CACTAAGTCCACGTCAAAGTATGATATCTACGCAAAATCAATATAGTACACTATCCAAACCAGCAGCACT
  AACAGGTACTTTGGAAGTTCGTCTTATGGGCTGCCAAGATATCCTAGAGAATGTCCCTGGACGGTCAAAA
  GCAACATCAGTTGCACTGCCTGGTTGGAGTCCAAGTGAAACCAGATCATCTTTCATGAGCAGAACGAGTA
  AAAGTAAAAGCGGAAGTAGTCGAAATCTTCTAAAAACCGATGACTTGTCCAATGATGTCTGTGCTGTTTT
  GAAGCTCGATAATACTGTGGTTGGCCAAACTAGCTGGAAACCCATTTCCAATCAGTCATGGGACCAGAAG
  TTTACACTGGAACTGGACAGGTCACGTGAACTGGAAATTTCAGTTTATTGGCGTGATTGGCGGTCTCTGT
  GTGCTGTAAAATTTCTGAGGTTAGAAGATTTTTTAGACAACCAACGGCATGGCATGTGTCTCTATTTGGA
  ACCACAGGGTACTTTATTTGCAGAGGTTACCTTTTTTAATCCAGTTATTGAAAGAAGACCAAAACTTCAA
  AGACAAAAGAAAATTTTTTCAAAGCAACAAGGCAAAACATTTCTCAGAGCTCCTCAAATGAATATTAATA
  TTGCCACTTGGGGAAGGCTAGTAAGAAGAGCTATTCCTACAGTAAATCATTCTGGCACCTTCAGCCCTCA
  AGCTCCTGTGCCTACTACAGTGCCAGTGGTTGATGTACGCATCCCTCAACTAGCACCTCCAGCTAGTGAT
  TCTACAGTAACCAAATTGGACTTTGATCTTGAGCCTGAACCTCCTCCAGCCCCACCACGAGCTTCTTCTC
  TTGGAGAAATAGATGAATCTTCTGAATTAAGAGTTTTGGATATACCAGGACAGGATTCAGAGACTGTTTT
  TGATATTCAGAATGACAGAAATAGTATACTTCCAAAATCTCAATCTGAATACAAGCCTGATACTCCTCAG
  TCAGGCCTAGAATATAGTGGTATTCAAGAACTTGAGGACAGAAGATCTCAGCAAAGGTTTCAGTTTAATC
  TACAAGATTTCAGGTGTTGTGCTGTCTTGGGAAGAGGACATTTTGGAAAGGTGCTTTTAGCTGAATATAA
  AAACACAAATGAGATGTTTGCTATAAAAGCCTTAAAGAAAGGAGATATTGTGGCTCGAGATGAAGTAGAC
  AGCCTGATGTGTGAAAAAAGAATTTTTGAAACTGTGAATAGTGTAAGGCATCCCTTTTTGGTGAACCTTT
  TTGCATGTTTCCAAACCAAAGAGCATGTTTGCTTTGTAATGGAATATGCTGCCGGTGGGGACCTAATGAT
  GCACATTCATACTGATGTCTTTTCTGAACCAAGAGCTGTATTTTATGCTGCTTGTGTAGTTCTTGGGTTG
  CAGTATTTACATGAACACAAAATTGTTTATAGAGATTTGAAATTGGATAACTTATTGCTAGATACAGAGG
  GCTTTGTGAAAATTGCTGATTTTGGTCTTTGCAAAGAAGGAATGGGATATGGAGATAGAACAAGCACATT
  TTGTGGCACTCCTGAATTTCTTGCCCCAGAAGTATTAACAGAAACTTCTTATACAAGGGCTGTAGATTGG
  TGGGGCCTTGGCGTGCTTATATATGAAATGCTTGTTGGTGAGTCTCCCTTTCCTGGTGATGATGAAGAGG
  AAGTTTTTGACAGTATTGTAAATGATGAAGTAAGGTATCCAAGGTTCTTATCTACAGAAGCCATTTCTAT
  AATGAGAAGGCTGTTAAGAAGAAATCCTGAACGGCGCCTTGGGGCTAGCGAGAAAGATGCAGAGGATGTA
  AAAAAGCACCCATTTTTCCGGCTAATTGATTGGAGCGCTCTGATGGACAAAAAAGTAAAGCCACCATTTA
  TACCTACCATAAGAGGACGAGAAGATGTTAGTAATTTTGATGATGAATTTACCTCAGAAGCACCTATTCT
  GACTCCACCTCGAGAACCAAGGATACTTTCGGAAGAGGAGCAGGAAATGTTCAGAGATTTTGACTACATT
  GCTGATTGGTGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220989 representing NM_006256
  Red=Cloning site Green=Tags(s)
  
  MASNPERGEILLTELQGDSRSLPFSENVSAVQKLDFSDTMVQQKLDDIKDRIKREIRKELKIKEGAENLR
  KVTTDKKSLAYVDNILKKSNKKLEELHHKLQELNAHIVVSDPEDITDCPRTPDTPNNDPRCSTSNNRLKA
  LQKQLDIELKVKQGAENMIQMYSNGSSKDRKLHGTAQQLLQDSKTKIEVIRMQILQAVQTNELAFDNAKP
  VISPLELRMEELRHHFRIEFAVAEGAKNVMKLLGSGKVTDRKALSEAQARFNESSQKLDLLKYSLEQRLN
  EVPKNHPKSRIIIEELSLVAASPTLSPRQSMISTQNQYSTLSKPAALTGTLEVRLMGCQDILENVPGRSK
  ATSVALPGWSPSETRSSFMSRTSKSKSGSSRNLLKTDDLSNDVCAVLKLDNTVVGQTSWKPISNQSWDQK
  FTLELDRSRELEISVYWRDWRSLCAVKFLRLEDFLDNQRHGMCLYLEPQGTLFAEVTFFNPVIERRPKLQ
  RQKKIFSKQQGKTFLRAPQMNINIATWGRLVRRAIPTVNHSGTFSPQAPVPTTVPVVDVRIPQLAPPASD
  STVTKLDFDLEPEPPPAPPRASSLGEIDESSELRVLDIPGQDSETVFDIQNDRNSILPKSQSEYKPDTPQ
  SGLEYSGIQELEDRRSQQRFQFNLQDFRCCAVLGRGHFGKVLLAEYKNTNEMFAIKALKKGDIVARDEVD
  SLMCEKRIFETVNSVRHPFLVNLFACFQTKEHVCFVMEYAAGGDLMMHIHTDVFSEPRAVFYAACVVLGL
  QYLHEHKIVYRDLKLDNLLLDTEGFVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTETSYTRAVDW
  WGLGVLIYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSTEAISIMRRLLRRNPERRLGASEKDAEDV
  KKHPFFRLIDWSALMDKKVKPPFIPTIRGREDVSNFDDEFTSEAPILTPPREPRILSEEEQEMFRDFDYI
  ADWC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006256
• ORF Size: 2952 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006256.1, NP_006247.1
• RefSeq Size: 3255 bp
• RefSeq ORF: 2955 bp
• Locus ID: 5586
• Cytogenetics: 1p22.2
• Domains: C2, pkinase, HR1, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: ''