GABRG3 (NM_033223) Human Tagged ORF Clone

CAT#: RG221044

GABRG3 (GFP-tagged) - Human gamma-aminobutyric acid (GABA) A receptor, gamma 3 (GABRG3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GABRG3
• Synonyms: gamma-aminobutyric acid (GABA) A receptor, gamma 3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221044 representing NM_033223
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCGAAGCTGCTGCTCCTCCTCTGCCTGTTCTCGGGCTTGCACGCGCGGTCCAGAAAGGTGGAAG
  AGGATGAATATGAAGATTCATCATCAAACCAAAAGTGGGTCTTGGCTCCAAAATCCCAAGACACCGACGT
  GACTCTTATTCTCAACAAGTTGCTAAGAGAGTATGATAAAAAGCTGAGGCCAGATATTGGAATAAAACCG
  ACCGTAATTGACGTTGACATTTATGTTAACAGCATTGGTCCTGTGTCATCAATAAACATGGAATACCAAA
  TTGACATATTTTTTGCTCAGACCTGGACAGATAGTCGCCTTCGATTCAACAGCACAATGAAAATTCTTAC
  TCTGAACAGCAACATGGTGGGGTTAATCTGGATCCCAGACACCATCTTCCGCAATTCTAAAACCGCAGAG
  GCTCACTGGATCACCACACCCAATCAGCTCCTCCGGATTTGGAATGACGGGAAAATCCTTTACACTTTGA
  GGCTCACCATCAATGCTGAGTGCCAGCTGCAGCTGCACAACTTCCCCATGGACGAACACTCCTGCCCGCT
  GATTTTCTCCAGCTATGGCTATCCCAAAGAAGAAATGATTTATAGATGGAGAAAAAATTCAGTGGAGGCA
  GCTGACCAGAAATCATGGCGGCTTTATCAGTTTGACTTCATGGGCCTCAGAAACACCACAGAAATCGTGA
  CAACGTCTGCAGGTGATTATGTTGTCATGACTATATATTTTGAATTGAGTAGAAGAATGGGATACTTCAC
  CATTCAGACATACATTCCCTGTATACTGACTGTGGTTTTATCCTGGGTGTCATTTTGGATCAAAAAAGAT
  GCTACGCCAGCAAGAACAGCATTAGGCATCACCACGGTGCTGACCATGACCACCCTGAGCACCATCGCCA
  GGAAGTCCTTGCCACGCGTGTCCTACGTGACCGCCATGGACCTTTTTGTGACTGTGTGCTTCCTGTTTGT
  CTTCGCCGCGCTGATGGAGTATGCCACCCTCAACTACTATTCCAGCTGTAGAAAACCAACCACCACGAAA
  AAGACAACATCGTTACTACATCCAGATTCCTCAAGATGGATTCCTGAGCGAATAAGCCTACAAGCCCCTT
  CCAACTATTCCCTCCTGGACATGAGGCCACCACCACCTGCGATGATCACTTTAAACAATTCCGTTTACTG
  GCAGGAATTTGAAGATACCTGTGTCTATGAGTGTCTGGATGGCAAAGACTGTCAGAGCTTCTTCTGCTGC
  TATGAAGAATGTAAATCAGGATCCTGGAGGAAAGGGCGTATTCACATAGACATCTTGGAGCTGGACTCGT
  ACTCCCGGGTCTTTTTCCCCACGTCCTTCCTGCTCTTTAACCTGGTCTACTGGGTTGGATACCTGTATCT
  C
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221044 representing NM_033223
  Red=Cloning site Green=Tags(s)
  
  MAPKLLLLLCLFSGLHARSRKVEEDEYEDSSSNQKWVLAPKSQDTDVTLILNKLLREYDKKLRPDIGIKP
  TVIDVDIYVNSIGPVSSINMEYQIDIFFAQTWTDSRLRFNSTMKILTLNSNMVGLIWIPDTIFRNSKTAE
  AHWITTPNQLLRIWNDGKILYTLRLTINAECQLQLHNFPMDEHSCPLIFSSYGYPKEEMIYRWRKNSVEA
  ADQKSWRLYQFDFMGLRNTTEIVTTSAGDYVVMTIYFELSRRMGYFTIQTYIPCILTVVLSWVSFWIKKD
  ATPARTALGITTVLTMTTLSTIARKSLPRVSYVTAMDLFVTVCFLFVFAALMEYATLNYYSSCRKPTTTK
  KTTSLLHPDSSRWIPERISLQAPSNYSLLDMRPPPPAMITLNNSVYWQEFEDTCVYECLDGKDCQSFFCC
  YEECKSGSWRKGRIHIDILELDSYSRVFFPTSFLLFNLVYWVGYLYL
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033223
• ORF Size: 1401 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033223.1, NP_150092.1
• RefSeq Size: 1536 bp
• RefSeq ORF: 1404 bp
• Locus ID: 2567
• Cytogenetics: 15q12
• Domains: Neur_chan_memb, Neur_chan_LBD
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: 'This gene encodes a gamma-aminobutyric acid (GABA) receptor. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. The protein encoded by this gene is a gamma subunit, which contains the benzodiazepine binding site. Two transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Aug 2012]'