CCDC132 (NM_024553) Human Tagged ORF Clone

CAT#: RG221086

CCDC132 (GFP-tagged) - Human coiled-coil domain containing 132 (CCDC132), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CCDC132
• Synonyms: CCDC132; VPS54L
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221086 representing NM_024553
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAAAAATCAAATCTCTCATGACCCGACAGGGTCTGAAAAGCCCTCAAGAAAGCCTCAGTGATCTTG
  GTGCCATAGAGAGTCTCCGGGTCCCTGGAAAGGAAGAATTCAGGGAACTTCGAGAACAGCCAAGTGACCC
  TCAAGCTGAACAAGAGCTTATTAATAGTATTGAACAAGTATATTTTTCTGTGGATTCATTTGATATTGTT
  AAATATGAGCTGGAGAAGCTTCCACCTGTTCTCAATTTGCAAGAATTAGAGGCGTATAGAGACAAATTGA
  AACAACAGCAAGCTGCAGTATCTAAAAAAGTGGCAGATTTAATCCTTGAAAAACAGCCTGCTTATGTAAA
  GGAACTTGAAAGAGTTACCTCATTGCAGACAGGTCTTCAATTAGCTGCTGTTATCTGTACAAATGGGAGA
  AGACACTTGAATATTGCAAAGGAAGGTTTTACTCAAGCTAGTTTAGGCCTTCTTGCAAATCAAAGGAAAC
  GTCAGTTGCTGATTGGACTTCTGAAATCTCTGAGAACTATAAAAACATTGCAAAGAACAGATGTACGGTT
  AAGTGAAATGCTGGAGGAGGAAGATTATCCAGGAGCTATTCAGTTGTGCCTTGAATGTCAAAAAGCTGCC
  AGCACTTTTAAACATTACAGTTGTATAAGTGAACTGAATTCAAAGCTGCAAGATACTTTGGAACAGATTG
  AGGAACAGCTGGACGTAGCTCTTTCCAAAATCTGCAAGAATTTTGACATTAACCATTATACCAAGGTTCA
  ACAAGCTTATCGACTTCTTGGAAAAACACAGACAGCAATGGATCAACTTCATATGCACTTCACCCAAGCC
  ATTCACAACACCGTGTTTCAAGTTGTTCTTGGTTATGTGGAACTATGTGCAGGAAACACAGACACAAAAT
  TCCAAAAGCTGCAATATAAGGATCTCTGTACACATGTTACACCAGACAGCTATATTCCATGCCTTGCAGA
  CCTGTGCAAAGCACTATGGGAAGTTATGCTCAGCTATTATAGGACTATGGAATGGCATGAAAAGCATGAC
  AATGAGGATACTGCTTCAGCTTCTGAAGGGAGTAATATGATAGGTACTGAAGAAACTAATTTTGATCGTG
  GCTACATAAAAAAGAAATTAGAACATGGACTTACACGAATATGGCAGGATGTTCAGCTAAAAGTAAAAAC
  CTACTTGCTTGGAACTGATTTGTCTATATTCAAATATGATGATTTCATCTTTGTTTTGGATATAATCAGC
  AGGTTGATGCAAGTTGGAGAAGAATTTTGTGGTAGCAAGTCTGAAGTTTTACAGGAATCTATTAGAAAAC
  AAAGTGTCAATTATTTCAAGAATTACCATAGAACACGGCTCGATGAACTGAGAATGTTCTTAGAGAATGA
  GACTTGGGAACTTTGTCCTGTTAAGTCAAATTTCAGCATCTTGCAACTTCATGAATTTAAATTCATGGAA
  CAGTCTCGCTCCCCATCAGTTTCACCTAGTAAACAGCCAGTCTCAACTTCTTCAAAAACAGTGACCTTGT
  TTGAGCAGTACTGTAGTGGTGGGAATCCATTTGAAATTCAGGCCAACCACAAAGATGAAGAAACAGAAGA
  TGTCTTAGCTTCTAATGGGTATGAATCTGATGAACAAGAAAAGAGTGCCTATCAAGAGTATGACAGTGAC
  AGTGATGTTCCTGAGGAACTCAAACGAGACTATGTGGATGAGCAGACAGGAGATGGTCCTGTGAAAAGTG
  TTTCTCGGGAAACTCTAAAAAGCAGGAAGAAATCAGATTACAGTCTAAATAAAGTGAATGCACCTATCTT
  AACAAATACAACATTGAACGTCATAAGACTTGTTGGAAAATATATGCAGATGATGAACATTCTTAAGCCA
  ATTGCCTTTGATGTTATTCATTTCATGTCTCAACTATTTGATTATTACTTGTATGCAATATATACCTTTT
  TTGGTCGGAATGATTCATTGGAATCAACTGGACTCGGCCTTAGTAGTAGTAGACTAAGAACAACTCTAAA
  CAGAATACAAGAAAGCCTTATTGATCTAGAAGTTTCAGCTGATCCTACTGCCACACTCACAGCAGCAGAA
  GAAAGAAAGGAGAAGGTGCCAAGTCCACACCTCAGTCACCTAGTGGTTTTGACATCTGGGGATACGTTGT
  ATGGGTTGGCAGAAAGAGTGGTAGCCACGGAATCCTTGGTATTCTTGGCTGAACAGTTTGAGTTCCTTCA
  GCCACATCTGGATGCTGTGATGCCTGCAGTCAAAAAGCCCTTTCTTCAGCAGTTCTATTCTCAGACAGTC
  TCAACCGCCAGTGAACTACGGAAACCAATTTACTGGATTGTAGCTGGTAAAGCCCTTGATTATGAACAGA
  TGCTGCTTCTCATGGCTAATGTGAAATGGGATGTAAAAGAAATTATGTCACAGCACAACATATATGTAGA
  TGCACTATTAAAGGAATTTGAGCAGTTTAACAGGAGGCTAAATGAAGTTTCTAAGAGAGTTCGCATACCC
  TTGCCTGTGTCTAATATACTTTGGGAACATTGTATACGATTGGCTAATCGAACTATTGTAGAAGGATATG
  CCAATGTCAAGAAATGCAGTAATGAGGGTCGTGCCCTGATGCAACTGGATTTTCAACAGTTTTTAATGAA
  ACTTGAAAAACTAACAGATATTAGACCCATTCCTGATAAAGAATTTGTAGAAACTTATATTAAAGCTTAT
  TACCTAACTGAGAATGACATGGAACGGTGGATCAAAGAGCACAGGGAATATTCAACGAAGCAGCTGACCA
  ATCTGGTGAATGTTTGCCTGGGATCCCATATCAATAAGAAAGCAAGACAAAAACTTCTAGCAGCTATAGA
  TGATATAGACAGACCTAAAAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221086 representing NM_024553
  Red=Cloning site Green=Tags(s)
  
  MQKIKSLMTRQGLKSPQESLSDLGAIESLRVPGKEEFRELREQPSDPQAEQELINSIEQVYFSVDSFDIV
  KYELEKLPPVLNLQELEAYRDKLKQQQAAVSKKVADLILEKQPAYVKELERVTSLQTGLQLAAVICTNGR
  RHLNIAKEGFTQASLGLLANQRKRQLLIGLLKSLRTIKTLQRTDVRLSEMLEEEDYPGAIQLCLECQKAA
  STFKHYSCISELNSKLQDTLEQIEEQLDVALSKICKNFDINHYTKVQQAYRLLGKTQTAMDQLHMHFTQA
  IHNTVFQVVLGYVELCAGNTDTKFQKLQYKDLCTHVTPDSYIPCLADLCKALWEVMLSYYRTMEWHEKHD
  NEDTASASEGSNMIGTEETNFDRGYIKKKLEHGLTRIWQDVQLKVKTYLLGTDLSIFKYDDFIFVLDIIS
  RLMQVGEEFCGSKSEVLQESIRKQSVNYFKNYHRTRLDELRMFLENETWELCPVKSNFSILQLHEFKFME
  QSRSPSVSPSKQPVSTSSKTVTLFEQYCSGGNPFEIQANHKDEETEDVLASNGYESDEQEKSAYQEYDSD
  SDVPEELKRDYVDEQTGDGPVKSVSRETLKSRKKSDYSLNKVNAPILTNTTLNVIRLVGKYMQMMNILKP
  IAFDVIHFMSQLFDYYLYAIYTFFGRNDSLESTGLGLSSSRLRTTLNRIQESLIDLEVSADPTATLTAAE
  ERKEKVPSPHLSHLVVLTSGDTLYGLAERVVATESLVFLAEQFEFLQPHLDAVMPAVKKPFLQQFYSQTV
  STASELRKPIYWIVAGKALDYEQMLLLMANVKWDVKEIMSQHNIYVDALLKEFEQFNRRLNEVSKRVRIP
  LPVSNILWEHCIRLANRTIVEGYANVKKCSNEGRALMQLDFQQFLMKLEKLTDIRPIPDKEFVETYIKAY
  YLTENDMERWIKEHREYSTKQLTNLVNVCLGSHINKKARQKLLAAIDDIDRPKR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024553
• ORF Size: 981 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_024553.2, NP_078829.1
• RefSeq Size: 1216
• RefSeq ORF: 984
• Locus ID: 55610
• Gene Summary: Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane. Within the EARP complex, required to tether the complex to recycling endosomes. Not involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). [UniProtKB/Swiss-Prot Function]