PDE4C (NM_001098819) Human Tagged ORF Clone

CAT#: RG221103

PDE4C (GFP-tagged) - Human phosphodiesterase 4C, cAMP-specific (PDE4C), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PDE4C
• Synonyms: DPDE1; PDE21
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221103 representing NM_001098819
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGCTCCAGTCCCGCACAGCCAGCGGCGCGAGTCCTTCCTGTACCGCTCAGATAGCGACTATGAAC
  TCTCGCCCAAGGCCATGTCTCGGAACTCCTCTGTGGCCAGCGACCTACATGGAGAGGACATGATTGTGAC
  GCCCTTTGCCCAGGTCCTGGCCAGTCTGCGGACCGTTCGGAGCAACGTGGCGGCCCTTGCCCGCCAGCAA
  TGCCTAGGAGCAGCCAAGCAGGGACCCGTCGGAAACCCTTCATCCAGCAATCAGCTCCCTCCTGCAGAGG
  ACACGGGGCAGAAGCTGGCATTGGAGACGCTAGACGAGCTGGACTGGTGCCTGGATCAGTTGGAGACGCT
  GCAGACCCGGCACTCGGTGGGGGAGATGGCCTCCAACAAGTTCAAGCGGATCCTGAACCGGGAGTTGACC
  CACCTGTCCGAAACCAGCCGCTCCGGGAACCAGGTGTCCGAGTACATCTCCCGGACCTTCCTGGACCAGC
  AGACCGAGGTGGAGCTGCCCAAGGTGACCGCTGAGGAGGCCCCACAGCCCATGTCCCGGATCAGTGGCCT
  ACATGGGCTCTGCCACAGTGCCAGCCTCTCCTCAGCCACTGTCCCACGCTTTGGGGTCCAGACTGACCAG
  GAGGAGCAACTGGCCAAGGAGCTAGAAGACACCAACAAGTGGGGACTTGATGTGTTCAAGGTGGCGGAGC
  TAAGTGGGAACCGGCCCCTCACAGCTATCATATTCAGCATTTTTCAGGAGCGGGACCTGCTGAAGACATT
  CCAGATCCCAGCAGACACACTGGCCACCTACCTGCTGATGCTGGAAGGTCACTACCACGCCAATGTGGCC
  TACCACAACAGCCTACATGCCGCCGACGTGGCCCAGTCCACGCATGTGCTGCTGGCTACGCCCGCCCTCG
  AGGCTGTGTTCACAGACTTGGAAATCCTGGCTGCCCTCTTTGCAAGCGCCATCCACGACGTGGACCATCC
  TGGGGTCTCCAACCAGTTTCTGATTAACACCAACTCAGAGCTGGCGCTTATGTACAACGACGCCTCGGTG
  CTGGAGAACCATCACCTGGCTGTGGGCTTCAAGCTGCTGCAGGCAGAGAACTGCGATATCTTCCAGAACC
  TCAGCGCCAAGCAGCGACTGAGTCTGCGCAGGATGGTCATTGACATGGTGCTGGCCACAGACATGTCCAA
  ACACATGAACCTCCTGGCCGACCTCAAGACCATGGTGGAGACCAAGAAGGTGACAAGCCTCGGTGTCCTC
  CTCCTGGACAACTATTCCGACCGAATCCAGGTCTTGCAGAACCTGGTGCACTGTGCTGATCTGAGCAACC
  CCACCAAGCCGCTGCCCCTGTACCGCCAGTGGACGGACCGCATCATGGCCGAGTTCTTCCAGCAGGGAGA
  CCGCGAGCGTGAGTCGGGCCTGGACATCAGTCCCATGTGTGACAAGCATACGGCCTCAGTGGAGAAGTCC
  CAGGTGGGTTTCATTGACTACATTGCTCACCCACTGTGGGAGACTTGGGCTGACCTGGTCCACCCAGATG
  CACAGGACCTGCTGGACACGCTGGAGGACAATCGAGAGTGGTACCAGAGCAAGATCCCCCGAAGTCCCTC
  AGACCTCACCAACCCCGAGCGGGACGGGCCTGACAGATTCCAGTTTGAACTGACTCTGGAGGAGGCAGAG
  GAAGAGGATGAGGAGGAAGAAGAGGAGGGGGAAGAGACAGCTTTAGCCAAAGAGGCCTTGGAGTTGCCTG
  ACACTGAACTCCTGTCCCCTGAAGCCGGCCCAGACCCTGGGGACTTACCCCTCGACAACCAGAGGACT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221103 representing NM_001098819
  Red=Cloning site Green=Tags(s)
  
  MQAPVPHSQRRESFLYRSDSDYELSPKAMSRNSSVASDLHGEDMIVTPFAQVLASLRTVRSNVAALARQQ
  CLGAAKQGPVGNPSSSNQLPPAEDTGQKLALETLDELDWCLDQLETLQTRHSVGEMASNKFKRILNRELT
  HLSETSRSGNQVSEYISRTFLDQQTEVELPKVTAEEAPQPMSRISGLHGLCHSASLSSATVPRFGVQTDQ
  EEQLAKELEDTNKWGLDVFKVAELSGNRPLTAIIFSIFQERDLLKTFQIPADTLATYLLMLEGHYHANVA
  YHNSLHAADVAQSTHVLLATPALEAVFTDLEILAALFASAIHDVDHPGVSNQFLINTNSELALMYNDASV
  LENHHLAVGFKLLQAENCDIFQNLSAKQRLSLRRMVIDMVLATDMSKHMNLLADLKTMVETKKVTSLGVL
  LLDNYSDRIQVLQNLVHCADLSNPTKPLPLYRQWTDRIMAEFFQQGDRERESGLDISPMCDKHTASVEKS
  QVGFIDYIAHPLWETWADLVHPDAQDLLDTLEDNREWYQSKIPRSPSDLTNPERDGPDRFQFELTLEEAE
  EEDEEEEEEGEETALAKEALELPDTELLSPEAGPDPGDLPLDNQRT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001098819
• ORF Size: 1818 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001098819.1, NP_001092289.1
• RefSeq Size: 5124 bp
• RefSeq ORF: 1821 bp
• Locus ID: 5143
• Cytogenetics: 19p13.11
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: 'The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]'