ARAP3 (NM_022481) Human Tagged ORF Clone

CAT#: RG221115

ARAP3 (GFP-tagged) - Human ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3 (ARAP3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ARAP3
• Synonyms: CENTD3; DRAG1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221115 representing NM_022481
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCCCCTCAGGACCTGGACATCGCTGTGTGGCTGGCCACGGTGCACCTGGAGCAGTATGCAGACA
  CGTTCCGACGGCATGGCCTGGCTACAGCAGGTGCAGCCCGGGGCCTGGGCCACGAGGAGTTGAAGCAGTT
  GGGCATCAGCGCCACAGGGCACCGGAAACGCATTCTACGCCTGCTACAGACAGGCACCGAAGAGGGCTCC
  CTGGATCCCAAATCAGATAGTGCCATGGAACCATCCCCCAGCCCAGCCCCGCAAGCCCAGCCCCCTAAGC
  CCGTGCCGAAGCCCAGGACCGTGTTTGGTGGACTCAGTGGCCCTGCCACCACTCAGAGACCTGGGCTGAG
  CCCAGCCCTCGGGGGACCAGGAGTGTCCAGGAGCCCAGAGCCCAGCCCAAGGCCTCCTCCTCTCCCCACT
  TCCTCCTCTGAGCAGTCTTCAGCCCTAAATACTGTGGAGATGATGCCTAATTCCATCTACTTCGGCCTGG
  ACTCAAGAGGCAGGGCACAGGCAGCTCAGGACAAGGCCCCAGACAGCTCCCAAATCTCTGCCCCCACCCC
  TGCCCTCAGGCCCACAACAGGCACAGTGCACATCATGGATCCTGGTTGCCTGTACTATGGTGTCCAACCT
  GTGGGGACTCCAGGAGCCCCCGACAGAAGAGAGAGCAGAGGTGTTTGTCAGGGCAGGGCTGAACACAGGC
  TCAGCAGACAGGATCTGGAGGCACGGGAGGATGCTGGCTATGCCAGCCTTGAGCTACCTGGAGACTCCAC
  CCTCTTATCGCCCACCCTGGAAACAGAGGAGACCAGTGATGACCTCATTTCACCCTATGCCAGCTTCTCC
  TTCACGGCAGACCGCCTCACGCCCCTGCTCAGTGGCTGGCTAGACAAGCTCTCCCCTCAGGGAAACTATG
  TCTTCCAGAGACGCTTTGTGCAGTTCAATGGGAGGAGTCTGATGTACTTTGGCAGTGACAAGGACCCCTT
  CCCTAAGGGTGTGATACCTTTGACTGCCATTGAGATGACCCGCAGCAGCAAGGACAACAAGTTCCAGGTC
  ATCACCGGCCAGAGGGTGTTCGTGTTCCGCACAGAGAGCGAGGCTCAGCGGGACATGTGGTGCTCCACGC
  TGCAGTCCTGTCTGAAGGAGCAGCGCCTCCTGGGCCACCCCCGGCCCCCCCAACCACCCCGACCCCTCCG
  CACGGGCATGCTGGAGCTGCGTGGACACAAGGCCAAGGTGTTTGCTGCCTTGAGCCCTGGAGAGCTGGCA
  CTGTACAAGAGTGAGCAGGCCTTCTCTCTGGGCATCGGGATCTGCTTCATCGAACTGCAGGGCTGCAGCG
  TCCGGGAGACCAAGAGTCGAAGCTTTGACCTGCTCACACCCCATCGCTGCTTCAGCTTCACAGCCGAGTC
  TGGGGGTGCTCGGCAGAGCTGGGCGGCCGCTCTGCAGGAAGCAGTAACCGAGACCCTGTCTGACTACGAG
  GTGGCTGAGAAGATCTGGTCTAATCGGGCCAACCGGCAGTGTGCGGACTGTGGGTCCTCCCGCCCAGATT
  GGGCTGCTGTCAATTTGGGGGTGGTCATCTGCAAGCAGTGTGCAGGTCAGCACCGGGCCCTGGGTTCTGG
  GATCTCCAAGGTGCAGAGCCTGAAGCTGGACACGAGTGTCTGGAGTAATGAGATAGTACAGTTATTCATT
  GTCCTGGGAAATGATCGTGCCAACCGCTTCTGGGCAGGGACCCTACCCCCAGGTGAGGGACTACATCCAG
  ATGCGACCCCTGGCCCCCGGGGAGAGTTCATCTCCCGAAAGTACCGTCTGGGTCTCTTCCGGAAGCCCCA
  CCCTCAGTACCCAGATCATAGCCAGCTTCTCCAGGCACTGTGTGCAGCTGTGGCAAGACCCAACCTGCTG
  AAGAACATGACCCAGCTCCTCTGTGTTGAGGCCTTTGAAGGCGAGGAGCCCTGGTTCCCCCCAGCCCCTG
  ATGGCAGCTGCCCTGGCCTCTTGCCCTCAGACCCCTCCCCTGGTGTGTACAATGAGGTGGTGGTGCGTGC
  TACTTACAGCGGCTTCCTGTACTGCAGTCCCGTCAGCAACAAAGCTGGACCCTCACCCCCTCGCAGGGGC
  CGGGATGCTCCCCCGCGCCTTTGGTGTGTGCTGGGAGCAGCTCTGGAAATGTTTGCATCGGAAAACAGCC
  CTGAACCCCTCAGCCTCATACAGCCCCAGGATATTGTATGTCTGGGTGTGAGCCCCCCACCCACTGACCC
  AGGTGACAGGTTCCCCTTTTCCTTTGAGCTCATCCTCGCTGGGGGGAGGATCCAGCATTTTGGCACAGAT
  GGAGCTGACAGTCTGGAGGCCTGGACTAGTGCTGTGGGCAAGTGGTTCTCCCCGCTGAGCTGCCACCAGC
  TGCTGGGCCCCGGGCTGCTGCGGCTGGGCCGCCTATGGCTGCGGTCCCCCTCCCATACAGCCCCGGCCCC
  TGGTCTCTGGCTGTCAGGGTTTGGCCTCCTTCGTGGTGACCACCTCTTCCTGTGCTCAGCGCCGGGCCCA
  GGCCCCCCAGCCCCTGAGGACATGGTGCATCTGCGGCGGCTACAGGAGATCAGTGTGGTTTCTGCAGCTG
  ACACCCCAGATAAGAAAGAGCATTTGGTCCTGGTGGAGACAGGAAGGACCCTGTATCTGCAAGGAGAGGG
  CCGGCTGGACTTCACGGCATGGAACGCAGCCATTGGGGGCGCGGCTGGTGGGGGCGGCACAGGGCTGCAG
  GAGCAGCAGATGAGCCGGGGTGACATCCCCATCATCGTGGATGCCTGCATCAGTTTTGTTACCCAGCATG
  GGCTCCGGCTGGAAGGTGTATACCGGAAAGGGGGCGCTCGTGCCCGCAGCCTGAGACTCCTGGCTGAGTT
  CCGTCGGGATGCCCGGTCGGTGAAGCTCCGACCAGGGGAGCACTTTGTGGAGGATGTCACTGACACACTC
  AAACGCTTCTTTCGTGAGCTCGATGACCCTGTGACCTCTGCACGGTTGCTGCCTCGCTGGAGGGAGGCTG
  CTGAGCTGCCCCAGAAGAATCAGCGCCTGGAGAAATATAAAGATGTGATTGGCTGCCTGCCGCGGGTCAA
  CCGCCGCACACTGGCCACCCTCATTGGGCATCTCTATCGGGTGCAGAAATGTGCGGCTCTAAACCAGATG
  TGCACGCGGAACTTGGCTCTGCTGTTTGCACCCAGCGTGTTCCAGACGGATGGGCGAGGGGAGCACGAGG
  TGCGAGTGCTGCAAGAGCTCATTGATGGCTACATCTCTGTCTTTGATATCGATTCTGACCAGGTAGCTCA
  GATTGACTTGGAGGTCAGTCTTATCACCACCTGGAAGGACGTGCAGCTGTCTCAGGCTGGAGACCTCATC
  ATGGAAGTTTATATAGAGCAGCAGCTCCCAGACAACTGTGTCACCCTGAAGGTGTCCCCAACCCTGACTG
  CTGAGGAGCTGACTAACCAGGTACTGGAGATGCGGGGGACAGCAGCTGGGATGGACTTGTGGGTGACTTT
  TGAGATTCGCGAGCATGGGGAGCTGGAGCGGCCACTGCATCCCAAGGAAAAGGTCTTAGAGCAGGCTTTA
  CAATGGTGCCAGCTCCCAGAGCCCTGCTCAGCTTCCCTGCTCTTGAAAAAAGTCCCCCTGGCCCAAGCTG
  GCTGCCTCTTCACAGGTATCCGACGTGAGAGCCCACGGGTGGGGCTGTTGCGGTGTCGTGAGGAGCCACC
  TCGCTTGCTGGGAAGCCGCTTCCAGGAGAGGTTCTTTCTGCTGCGTGGCCGCTGCCTGCTGCTGCTCAAG
  GAGAAGAAAAGCTCTAAACCAGAACGGGAGTGGCCTTTGGAAGGTGCCAAGGTCTACCTGGGAATCCGCA
  AGAAGTTAAAGCCCCCAACACCGTGGGGCTTCACATTGATACTAGAGAAGATGCACCTCTACTTGTCCTG
  CACTGACGAGGATGAAATGTGGGATTGGACCACCAGCATCCTTAAAGCCCAGCACGATGACCAGCAGCCA
  GTGGTCTTACGACGCCATTCCTCCTCTGACCTTGCCCGTCAGAAGTTTGGCACTATGCCTTTGCTGCCTA
  TCCGTGGGGATGACAGTGGAGCCACCCTCCTCTCTGCCAATCAGACCCTGCGGCGACTACACAACCGGAG
  GACCCTGTCCATGTTCTTTCCAATGAAGTCATCCCAGGGGTCTGTGGAGGAGCAAGAGGAGCTGGAGGAG
  CCTGTGTACGAGGAGCCAGTGTATGAGGAAGTAGGGGCCTTCCCTGAGTTGATCCAGGACACTTCTACCT
  CCTTCTCCACCACACGGGAGTGGACAGTGAAGCCAGAGAACCCCCTCACCAGCCAGAAGTCATTGGATCA
  ACCCTTTCTCTCCAAGTCAAGCACCCTTGGCCAGGAGGAGAGGCCACCTGAGCCCCCTCCAGGCCCCCCT
  TCAAAGAGCAGTCCCCAGGCACGGGGGTCCCTAGAGGAACAGCTGCTCCAGGAGCTCAGCAGCCTCATCC
  TGAGGAAAGGAGAGACCACTGCAGGCCTGGGAAGTCCTTCCCAGCCATCCAGCCCCCAATCCCCCAGCCC
  CACTGGCCTTCCAACACAGACACCTGGCTTCCCCACCCAACCCCCATGCACTTCCAGTCCACCCTCCAGC
  CAGCCCCTCACA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221115 representing NM_022481
  Red=Cloning site Green=Tags(s)
  
  MAAPQDLDIAVWLATVHLEQYADTFRRHGLATAGAARGLGHEELKQLGISATGHRKRILRLLQTGTEEGS
  LDPKSDSAMEPSPSPAPQAQPPKPVPKPRTVFGGLSGPATTQRPGLSPALGGPGVSRSPEPSPRPPPLPT
  SSSEQSSALNTVEMMPNSIYFGLDSRGRAQAAQDKAPDSSQISAPTPALRPTTGTVHIMDPGCLYYGVQP
  VGTPGAPDRRESRGVCQGRAEHRLSRQDLEAREDAGYASLELPGDSTLLSPTLETEETSDDLISPYASFS
  FTADRLTPLLSGWLDKLSPQGNYVFQRRFVQFNGRSLMYFGSDKDPFPKGVIPLTAIEMTRSSKDNKFQV
  ITGQRVFVFRTESEAQRDMWCSTLQSCLKEQRLLGHPRPPQPPRPLRTGMLELRGHKAKVFAALSPGELA
  LYKSEQAFSLGIGICFIELQGCSVRETKSRSFDLLTPHRCFSFTAESGGARQSWAAALQEAVTETLSDYE
  VAEKIWSNRANRQCADCGSSRPDWAAVNLGVVICKQCAGQHRALGSGISKVQSLKLDTSVWSNEIVQLFI
  VLGNDRANRFWAGTLPPGEGLHPDATPGPRGEFISRKYRLGLFRKPHPQYPDHSQLLQALCAAVARPNLL
  KNMTQLLCVEAFEGEEPWFPPAPDGSCPGLLPSDPSPGVYNEVVVRATYSGFLYCSPVSNKAGPSPPRRG
  RDAPPRLWCVLGAALEMFASENSPEPLSLIQPQDIVCLGVSPPPTDPGDRFPFSFELILAGGRIQHFGTD
  GADSLEAWTSAVGKWFSPLSCHQLLGPGLLRLGRLWLRSPSHTAPAPGLWLSGFGLLRGDHLFLCSAPGP
  GPPAPEDMVHLRRLQEISVVSAADTPDKKEHLVLVETGRTLYLQGEGRLDFTAWNAAIGGAAGGGGTGLQ
  EQQMSRGDIPIIVDACISFVTQHGLRLEGVYRKGGARARSLRLLAEFRRDARSVKLRPGEHFVEDVTDTL
  KRFFRELDDPVTSARLLPRWREAAELPQKNQRLEKYKDVIGCLPRVNRRTLATLIGHLYRVQKCAALNQM
  CTRNLALLFAPSVFQTDGRGEHEVRVLQELIDGYISVFDIDSDQVAQIDLEVSLITTWKDVQLSQAGDLI
  MEVYIEQQLPDNCVTLKVSPTLTAEELTNQVLEMRGTAAGMDLWVTFEIREHGELERPLHPKEKVLEQAL
  QWCQLPEPCSASLLLKKVPLAQAGCLFTGIRRESPRVGLLRCREEPPRLLGSRFQERFFLLRGRCLLLLK
  EKKSSKPEREWPLEGAKVYLGIRKKLKPPTPWGFTLILEKMHLYLSCTDEDEMWDWTTSILKAQHDDQQP
  VVLRRHSSSDLARQKFGTMPLLPIRGDDSGATLLSANQTLRRLHNRRTLSMFFPMKSSQGSVEEQEELEE
  PVYEEPVYEEVGAFPELIQDTSTSFSTTREWTVKPENPLTSQKSLDQPFLSKSSTLGQEERPPEPPPGPP
  SKSSPQARGSLEEQLLQELSSLILRKGETTAGLGSPSQPSSPQSPSPTGLPTQTPGFPTQPPCTSSPPSS
  QPLT
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022481
• ORF Size: 4632 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022481.5, NP_071926.4
• RefSeq Size: 5281
• RefSeq ORF: 4635
• Locus ID: 64411
• Protein Pathways: Endocytosis
• Gene Summary: This gene encodes a phosphoinositide binding protein containing ARF-GAP, RHO-GAP, RAS-associating, and pleckstrin homology domains. The ARF-GAP and RHO-GAP domains cooperate in mediating rearrangements in the cell cytoskeleton and cell shape. It is a specific PtdIns(3,4,5)P3/PtdIns(3,4)P2-stimulated Arf6-GAP protein. An alternatively spliced transcript has been found for this gene, but its biological validity has not been determined. [provided by RefSeq, Sep 2015]