CTAGE5 (MIA2) (NM_203355) Human Tagged ORF Clone

CAT#: RG221348

CTAGE5 (GFP-tagged) - Human CTAGE family, member 5 (CTAGE5), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MIA2
• Synonyms: CTAGE5; MEA6; MGEA; MGEA6; MGEA11; TALI
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221348 representing NM_203355
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGCCCGGGGTTACCCCTCAACCGTATTTGGGGCTGCTCCTGGAGGAGCTACGCAGGGTTGTGG
  CAGCACTGCCTGAAGGTATGAGACCAGATTCTAATCTTTATGGTTTTCCATGGGAATTGGTGATATGTGC
  AGCTGTTGTTGGATTTTTTGCTGTTCTCTTTTTTTTGTGGAGAAGTTTTAGATCGGTTAGGAGTCGGCTT
  TATGTGGGACGAGAGAAAAAGCTTGCTCTAATGCTTTCTGGACTAATTGAAGAAAAAAGTAAACTACTTG
  AAAAATTTAGCCTTGTTCAAAAAGAGTATGAAGGCTATGAAGTAGAGTCATCTTTAAAGGATGCCAGCTT
  TGAGAAGGAGGCAACAGAAGCACAAAGTTTGGAGGCAACCTGTGAAAAGCTGAACAGGTCCAATTCTGAA
  CTTGAGGATGAAATACTCTGTCTAGAAAAAGAGTTAAAAGAAGAGAAATCCAAACATTCTGAACAAGATG
  AATTGATGGCGGATATTTCAAAAAGGATACAGTCTCTAGAAGATGAGTCAAAATCCCTCAAATCACAAGT
  AGCTGAAGCCAAAATGACCTTCAAGATATTTCAAATGAATGAAGAACGACTGAAGATAGCAATAAAAGAT
  GCTTTGAATGAAAATTCTCAACTTCAGGAAAGCCAGAAACAGCTTTTGCAAGAAGCTGAAGTATGGAAAG
  AACAAGTGAGTGAACTTAATAAACAGAAAGTAACATTTGAAGACTCCAAAGTACATGCAGAACAAGTTCT
  AAATGATAAAGAAAGTCACATCAAGACTCTGACTGAACGCTTGTTAAAGATGAAAGATTGGGCTGCTATG
  CTTGGAGAAGACATAACGGATGATGATAACTTGGAATTAGAAATGAACAGTGAATCGGAAAATGGTGCTT
  ACTTAGATAATCCTCCAAAAGGAGCTTTGAAGAAACTGATTCATGCTGCTAAGTTAAATGCTTCTTTAAA
  AACCTTAGAAGGAGAAAGAAACCAAATTTATATTCAGTTGTCTGAAGTTGATAAAACAAAGGAAGAGCTT
  ACAGAGCATATTAAAAATCTTCAGACTGAACAAGCATCTTTGCAGTCAGAAAACACACATTTTGAAAATG
  AGAATCAGAAGCTTCAACAGAAACTTAAAGTAATGACTGAATTATATCAAGAAAATGAAATGAAACTCCA
  CAGGAAATTAACAGTAGAGGAAAATTATCGGTTAGAGAAAGAAGAGAAACTTTCTAAAGTAGATGAAAAG
  ATCAGCCATGCCACTGAAGAGCTGGAGACCTATAGAAAGCGAGCCAAAGATCTTGAAGAAGAATTGGAGA
  GAACTATTCATTCTTATCAAGGGCAGATTATTTCCCATGAGAAAAAAGCACATGATAATTGGTTGGCAGC
  TCGGAATGCTGAAAGAAACCTCAATGATTTAAGGAAAGAAAATGCTCACAACAGACAAAAATTAACTGAA
  ACAGAGCTTAAATTTGAACTTTTAGAAAAAGATCCTTATGCACTCGATGTTCCAAATACAGCATTTGGCA
  GAGGCTCACGAGGCCCAGGGAATCCTCTGGACCATCAGATTACCAATGAAAGAGGAGAATCAAGCTGTGA
  TAGGTTAACCGATCCTCATAGGGCTCCCTCTGACACTGGGTCTCTGTCACCTCCATGGGACCAGGACCGT
  AGGATGATGTTTCCTCCGCCAGGACAATCATATCCTGATTCAGCCCTTCCTCCACAAAGGCAAGACAGAT
  TTTGTTCTAATTCTGGTAGACTGTCTGGACCAGCAGAACTCAGAAGTTTTAATATGCCTTCTTTGGATAA
  AATGGATGGGTCAATGCCTTCAGAAATGGAATCCAGTAGAAATGATACCAAAGATGATCTTGGTAATTTA
  AATGTGCCTGATTCATCTCTCCCTGCTGAAAATGAAGCCACTGGCCCTGGCTTTGTTCCTCCACCTCTTG
  CTCCAATCAGAGGTCCATTGTTTCCAGTGGATGCAAGAGGCCCATTCTTGAGAAGAGGACCTCCTTTCCC
  CCCACCTCCTCCAGGAGCCATGTTTGGAGCTTCTCGAGATTATTTTCCACCAGGGGATTTCCCAGGTCCA
  CCACCTGCTCCATTTGCAATGAGAAATGTCTATCCACCGAGGGGTTTTCCTCCTTACCTTCCCCCAAGAC
  CTGGATTTTTCCCCCCACCCCCACATTCTGAAGGTAGAAGTGAGTTCCCCTCAGGTTTGATTCCACCTTC
  AAATGAGCCTGCTACTGAACATCCAGAACCACAGCAAGAAACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221348 representing NM_203355
  Red=Cloning site Green=Tags(s)
  
  MEEPGVTPQPYLGLLLEELRRVVAALPEGMRPDSNLYGFPWELVICAAVVGFFAVLFFLWRSFRSVRSRL
  YVGREKKLALMLSGLIEEKSKLLEKFSLVQKEYEGYEVESSLKDASFEKEATEAQSLEATCEKLNRSNSE
  LEDEILCLEKELKEEKSKHSEQDELMADISKRIQSLEDESKSLKSQVAEAKMTFKIFQMNEERLKIAIKD
  ALNENSQLQESQKQLLQEAEVWKEQVSELNKQKVTFEDSKVHAEQVLNDKESHIKTLTERLLKMKDWAAM
  LGEDITDDDNLELEMNSESENGAYLDNPPKGALKKLIHAAKLNASLKTLEGERNQIYIQLSEVDKTKEEL
  TEHIKNLQTEQASLQSENTHFENENQKLQQKLKVMTELYQENEMKLHRKLTVEENYRLEKEEKLSKVDEK
  ISHATEELETYRKRAKDLEEELERTIHSYQGQIISHEKKAHDNWLAARNAERNLNDLRKENAHNRQKLTE
  TELKFELLEKDPYALDVPNTAFGRGSRGPGNPLDHQITNERGESSCDRLTDPHRAPSDTGSLSPPWDQDR
  RMMFPPPGQSYPDSALPPQRQDRFCSNSGRLSGPAELRSFNMPSLDKMDGSMPSEMESSRNDTKDDLGNL
  NVPDSSLPAENEATGPGFVPPPLAPIRGPLFPVDARGPFLRRGPPFPPPPPGAMFGASRDYFPPGDFPGP
  PPAPFAMRNVYPPRGFPPYLPPRPGFFPPPPHSEGRSEFPSGLIPPSNEPATEHPEPQQET
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_203355
• ORF Size: 2283 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_203355.2, NP_976230.1
• RefSeq Size: 3551 bp
• RefSeq ORF: 2286 bp
• Locus ID: 4253
• Cytogenetics: 14q21.1
• Protein Families: Transmembrane
• Gene Summary: 'This gene encodes s receptor in the endoplasmic reticulum, which plays a role in the export of large pre-chylomicrons and pre-very low density lipoproteins (pre-VLDLs). Three major classes of transcripts are generated from this gene- melanoma inhibitory activity 2-specific transcripts, cTAGE family member 5-specific transcripts and transcripts that include exons from both these transcript species (TANGO1-like or TALI). Additionally, alternative splicing in these transcripts results in multiple transcript variants encoding multiple isoforms. [provided by RefSeq, Sep 2016]'