GARNL1 (RALGAPA1) (NM_194301) Human Tagged ORF Clone

CAT#: RG221378

RALGAPA1 (GFP-tagged) - Human Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RALGAPA1
• Synonyms: GARNL1; GRIPE; p240; RalGAPalpha1; TULIP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221378 representing NM_194301
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTCCAAGAAGCCGCACGGGGACGTGAAGAAGTCCACCCAGAAGGTGCTAGACACCAAGAAGGACG
  CACTGACTCGCCTCAAGCACCTGCGCATCGTCATCGAGAATGCAGAATCTATTGATCTTAAACAGTTTTT
  CGACCAACATTTTTCACATATATACTATGTGTTCTTTGAAAATTTTGTGACTATTGAAGCTAGTCTTAAA
  CAGAAAGGTCACAAGTCTCAAAGAGAGGAATTGGATGCTATACTTTTTATTTTTGAGAAAATTTTACAAC
  TTCTTCCAGAAAGAATTCATCAGCGATGGCAGTTTCATAGTATTGGATTGATTTTAAAGAAGCTACTTCA
  CACAGGAAACTCCTTAAAGATTAGGCGGGAAGGTGTTCGTCTTTTCTTACTATGGTTGCAAGCTCTTCAG
  AATAACTGTAGCAAAGAACAGCTCTGGATGTTTTCATGCTTAATCCCTGGATTTTCAGCACCACAGTCTG
  AACATGGACCTCGAACTTTAGATAATCTCATTAATCCTCCACTCAACCTTCAAGAAACTCAAGTCACTAT
  AGAAGAAATCACTCCTCTTGTCCCCCCACAATCAGGAGATAAAGGGCAAGAAGATCTCACAAGCTATTTT
  CTTGAAGCACTTCTAAAATACATAGTCATTCAGGTAAAAAGTTTAGAATGGAAGAACAAAGAAAACCAAG
  AAAGGGGATTTTCATTTTTGTTTTCACATTTTAAGAAATATTACTTGCCTTATATTTTTCCAAACATCTG
  TAAGGAAAACAGTTTATATCATCCTATACTTGACATCCCGCAGATGAGACCAAAGCCACATTATGTCGTG
  ATAAAGAAAGATGCTGAAACCAATGAAGCAATCTATTGTACAAAGGAGCCTTTCATTAAGGCTCGTGTTA
  TTGTCATTCGTTGGCTGGTTTCTTTCTGGCTGGAGCCAAAACCACATACAGGACCTCATATTCCTGGGAT
  GGAAGGTGAAGTCTTGCCAAAGAATATTCAGAGAGCAGCTGCTAGTTTAGTATCCAGAGAAGAAAGCAAA
  AATGATAATGCTGATAAAACAGACAGAACTACAGAACCCGAACAGTCTCATTCCAATACAAGCACTCTCA
  CGGAGCGAGAACCTAGCTCATCTAGTCTCTGTAGTATTGATGAAGAACATCTCACAGACATTGAAATAGT
  TCGCAGAGTTTTTTCTTCTAAAAGGAGTAATGTAAACTTTGTGACAGAGATATTTCGTCAGGCATTTTTA
  TTACCAATTTGTGAAGCAGCAGCTATGAGAAAAGTGGTAAAAGTATATCAAGAATGGATCCAACAAGAGG
  AAAAACCTTTGTTCATGCAAGAGCCTGAAGAAATTGTGATCACTTCTTCAGACCTCCCTTGCATTGAAAA
  TGTCACAGACCATGATATTTCAATGGAAGAAGGAGAAAAAAGAGAAGAGGAAAATGGGACCAATACTGCT
  GATCATGTTCGAAATTCCAGTTGGGCAAAAAACGGCTCCTACCAAGGTGCTCTTCATAACGCCTCTGAAG
  AAGCCACAGAACAAAACATACGAGCTGGTACCCAGGCAGTTTTGCAGGTGTTTATTATAAACTCATCAAA
  TATATTTCTTCTTGAACCTGCAAATGAAATAAAAAATCTTCTGGATGAACACACAGATATGTGTAAACGC
  ATTCTTAACATTTATCGGTACATGGTTGTACAAGTATCAATGGACAAAAAGACTTGGGAACAGATGCTGC
  TTGTGTTGCTCAGAGTCACGGAATCTGTACTGAAGATGCCATCACAAGCTTTTCTACAGTTCCAAGGGAA
  AAAAAATATGACCTTGGCAGGTCGACTTGCAGGACCACTTTTCCAGACCCTTATAGTTGCCTGGATCAAA
  GCAAACCTAAATGTGTACATCTCCCGAGAACTTTGGGATGACTTACTGTCAGTATTGTCATCGCTGACCT
  ATTGGGAAGAGTTGGCCACTGAGTGGTCACTGACTATGGAGACATTAACTAAAGTTTTAGCTAGGAATTT
  ATATAGTTTGGATCTCAGTGATTTACCATTGGATAAGCTGAGTGAACAGAAACAAAAAAAGCACAAAGGG
  AAAGGAGTTGGACATGAATTTCAGAAAGTTTCAGTTGACAAGTCATTTTCTAGAGGATGGAGTCGTGATC
  AGCCTGGCCAAGCCCCAATGAGACAGAGGAGTGCAACAACCACTGGTTCTCCAGGAACCGAAAAGGCGAG
  GAGTATAGTACGGCAAAAAACTGTCGATATTGATGATGCTCAAATACTTCCCCGCTCAACTAGAGTCAGA
  CATTTTTCACAAAGTGAAGAAACTGGAAATGAAGTTTTTGGTGCTTTGAATGAGGAGCAGCCATTGCCTC
  GAAGTAGCAGCACTTCTGACATCTTGGAACCATTCACTGTTGAACGAGCCAAAGTCAATAAAGAGGACAT
  GAGCCAAAAACTGCCTCCTCTTAATAGTGATATTGGCGGCAGCAGTGCTAATGTTCCTGATCTGATGGAT
  GAGTTTATAGCAGAACGACTTCGAAGTGGTAATGCCTCGACTATGACAAGAAGAGGAAGTAGTCCAGGCA
  GCCTGGAAATTCCCAAAGACCTCCCTGATATTCTAAACAAGCAGAACCAGATGCGCCCTATTGATGACCC
  AGGTGTGCCCTCAGAATGGACTTCTCCTGCCAGTGCAGGGAGCAGTGATCTTATCAGCTCAGATAGTCAT
  TCGGATTCTTTCAGCGCTTTCCAATATGATGGCCGAAAATTTGACAATTTTGGCTTTGGAACCGACACTG
  GGGTTACGTCCTCTGCTGATGTGGATTCAGGTTCTGGCCATCATCAGAGTGCTGAAGAGCAGGAAGTGGC
  TAGTCTAACTACTCTTCATATAGATTCTGAAACAAGCAGTCTTAATCAGCAAGCTTTCTCTGCTGAAGTT
  GCAACTATTACTGGTTCAGAAAGTGCTTCCCCAGTCCACTCACCTCTGGGCTCCAGGTCACAGACTCCTT
  CCCCTTCTACATTGAATATAGATCACATGGAACAGAAGGATCTGCAGCTCGACGAGAAGCTCCACCACTC
  TGTTCTTCAGACACCAGATGATCTAGAAATTAGTGAATTTCCATCAGAATGTTGTAGTGTGATGGCAGGA
  GGTACTCTGACTGGATGGCATGCTGATGTTGCTACTGTAATGTGGCGAAGAATGCTAGGCATTTTGGGAG
  ATGTAAATTCAATCATGGATCCTGAAATACATGCTCAAGTTTTTGATTACCTCTGTGAACTTTGGCAGAA
  TCTAGCTAAGATTAGAGATAACCTTGGCATTTCAACTGATAACCTGACCTCCCCTTCTCCACCAGTTTTA
  ATTCCTCCACTGAGAATTCTTACACCTTGGCTTTTTAAGGCAACCATGTTGACTGATAAATATAAACAAG
  GTAAATTACATGCATATAAACTTATTTGTAATACAATGAAAAGAAGACAAGATGTTTCTCCAAATAGAGA
  TTTTCTAACACATTTCTACAATATAATGCATTGTGGATTACTTCATATTGACCAGGATATTGTCAATACA
  ATCATCAAACACTGCTCACCTCAATTTTTTTCACTTGGTTTGCCTGGTGCCACAATGCTTATTATGGATT
  TTATTGTAGCAGCTGGTAGAGTGGCTTCTTCAGCTTTTCTCAATGCACCAAGAGTAGAAGCACAAGTTCT
  TCTGGGATCTTTGGTTTGCTTTCCCAACTTATATTGTGAACTGCCTTCTCTTCATCCCAACATTCCTGAT
  GTTGCTGTGTCTCAGTTTACAGATGTTAAGGAACTTATAATCAAAACTGTATTAAGCTCGGCAAGAGATG
  AGCCCTCTGGTCCTGCACGATGTGTAGCACTTTGTAGTTTAGGTATTTGGATTTGTGAAGAACTAGTCCA
  TGAGTCTCATCATCCTCAAATTAAGGAAGCTCTGAATGTGATTTGTGTTTCCTTAAAGTTTACTAATAAA
  ACAGTAGCCCACGTAGCTTGTAACATGCTTCACATGCTGGTTCATTATGTACCTAGACTTCAGATTTACC
  AGCCTGATTCTCCCTTGAAAATTATTCAAATCCTAATAGCTACCATCACCCATCTTTTACCAAGTACAGA
  GGCTTCATCTTATGAAATGGACAAGAGGTTGGTAGTATCTTTACTTCTCTGCCTTCTGGACTGGATCATG
  GCCTTACCTCTAAAGACACTGCTCCAACCATTTCATGCTACGGGAGCAGAAAGCGATAAAACAGAAAAAT
  CTGTTCTCAATTGCATTTATAAGGTTTTACATGGGTGTGTTTATGGAGCTCAGTGTTTTAGCAATCCAAG
  GTATTTTCCCATGAGCCTCTCTGATTTGGCATCTGTAGATTATGATCCTTTTATGCATTTGGAAAGTCTG
  AAAGAGCCTGAGCCTCTGCACTCTCCTGACTCAGAACGATCTTCTAAACTCCAGCCAGTAACAGAAGTGA
  AAACTCAAATGCAGCATGGATTAATCTCTATAGCAGCCCGCACTGTTATTACACATCTGGTAAATCACCT
  GGGCCATTATCCAATGAGCGGTGGTCCTGCTATGCTAACAAGTCAGGTGTGTGAAAATCACGACAATCAT
  TACAGTGAAAGTACTGAACTTTCTCCTGAACTCTTTGAGAGTCCAAATATCCAGTTCTTTGTGTTAAATA
  ATACAACCTTAGTGTCCTGTATCCAGATCAGATCAGAAGAGAATATGCCTGGAGGAGGTTTATCTGCTGG
  CCTTGCATCAGCCAATTCAAATGTCAGAATCATAGTACGTGATCTCTCTGGAAAATATTCATGGGATTCT
  GCTATACTGTATGGCCCACCTCCTGTAAGTGGCTTGTCAGAACCTACATCTTTCATGCTTTCATTGTCTC
  ACCAAGAGAAGCCAGAAGAGCCTCCGACATCTAATGAATGCTTAGAAGATATAACCGTAAAAGATGGACT
  TTCTCTCCAGTTTAAAAGATTTAGAGAAACTGTACCAACTTGGGATACAATAAGAGATGAAGAAGATGTT
  CTTGATGAGCTCTTGCAGTATTTGGGTGTTACTAGTCCTGAATGCTTACAGAGAACTGGAATCTCACTTA
  ATATTCCTGCTCCACAACCTGTGTGCATTTCTGAAAAACAAGAAAATGATGTTATTAATGCTATCCTTAA
  GCAACATACAGAAGAAAAAGAATTTGTTGAGAAGCACTTTAATGACTTAAACATGAAAGCTGTGGAACAA
  GATGAACCAATACCTCAAAAACCTCAGTCAGCATTTTATTATTGCAGATTGCTTCTTAGTATATTGGGAA
  TGAATTCCTGGGACAAACGGAGGAGCTTTCATCTCCTGAAGAAAAATGAAAAGCTACTTAGAGAACTTAG
  GAACTTGGATTCAAGGCAGTGCCGAGAGACACACAAGATTGCAGTATTTTATGTTGCTGAAGGACAAGAA
  GACAAACACTCCATTCTCACCAATACAGGAGGAAGTCAAGCATATGAAGATTTTGTAGCTGGTCTTGGTT
  GGGAGGTAAATCTTACAAACCATTGTGGTTTTATGGGAGGACTACAAAAAAACAAAAGCACTGGATTGAC
  CACTCCATATTTTGCTACCTCTACAGTAGAGGTAATATTTCACGTGTCAACAAGAATGCCTTCTGATTCT
  GATGATTCTTTGACCAAAAAATTGAGACATTTGGGAAATGATGAAGTGCACATTGTTTGGTCAGAGCATA
  CTAGAGACTACAGGAGAGGAATTATTCCCACAGAATTTGGTGATGTCCTTATTGTAATATATCCAATGAA
  AAATCACATGTTCAGTATTCAGATAATGAAAAAACCAGAGGTTCCCTTCTTTGGTCCCCTTTTTGATGGT
  GCTATTGTGAATGGAAAGGTTCTACCCATTATGGTTAGAGCAACAGCTATAAATGCAAGCCGTGCTCTGA
  AATCTCTGATTCCATTGTATCAAAACTTCTATGAGGAGAGAGCACGATACCTGCAAACAATTGTCCAGCA
  CCACTTAGAACCAACAACATTTGAAGATTTTGCAGCACAGGTTTTTTCTCCAGCTCCCTACCACCATTTA
  CCATCTGATGCCGGCTCCTACCCAGAGATTCTACCCAGTGAAACTCCCACAGCAACGCAGGTAGATGGGG
  CTGACCTGGCCTCTCCAATGTCTCCTCGAACTAGCAAAAGCCGCATGTCCATGAAGCTGCGTCGTTCCTC
  TGGCTCAGCCAATAAATCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221378 representing NM_194301
  Red=Cloning site Green=Tags(s)
  
  MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLK
  QKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQ
  NNCSKEQLWMFSCLIPGFSAPQSEHGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYF
  LEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPILDIPQMRPKPHYVV
  IKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREESK
  NDNADKTDRTTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFL
  LPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVITSSDLPCIENVTDHDISMEEGEKREEENGTNTA
  DHVRNSSWAKNGSYQGALHNASEEATEQNIRAGTQAVLQVFIINSSNIFLLEPANEIKNLLDEHTDMCKR
  ILNIYRYMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIK
  ANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKG
  KGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRSTRVR
  HFSQSEETGNEVFGALNEEQPLPRSSSTSDILEPFTVERAKVNKEDMSQKLPPLNSDIGGSSANVPDLMD
  EFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSH
  SDSFSAFQYDGRKFDNFGFGTDTGVTSSADVDSGSGHHQSAEEQEVASLTTLHIDSETSSLNQQAFSAEV
  ATITGSESASPVHSPLGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAG
  GTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVL
  IPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNT
  IIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPSLHPNIPD
  VAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNK
  TVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIM
  ALPLKTLLQPFHATGAESDKTEKSVLNCIYKVLHGCVYGAQCFSNPRYFPMSLSDLASVDYDPFMHLESL
  KEPEPLHSPDSERSSKLQPVTEVKTQMQHGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNH
  YSESTELSPELFESPNIQFFVLNNTTLVSCIQIRSEENMPGGGLSAGLASANSNVRIIVRDLSGKYSWDS
  AILYGPPPVSGLSEPTSFMLSLSHQEKPEEPPTSNECLEDITVKDGLSLQFKRFRETVPTWDTIRDEEDV
  LDELLQYLGVTSPECLQRTGISLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAVEQ
  DEPIPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQE
  DKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDS
  DDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDG
  AIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHL
  PSDAGSYPEILPSETPTATQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_194301
• ORF Size: 6249 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_194301.2, NP_919277.2
• RefSeq Size: 7851
• RefSeq ORF: 6252
• Locus ID: 253959
• Gene Summary: This gene encodes a major subunit of the RAL-GTPase activating protein. A similar protein in mouse binds E12, a transcriptional regulator of immunoglobulin genes. The mouse protein also functions in skeletal muscle by binding to the regulatory 14-3-3 proteins upon stimulation with insulin or muscle contraction. A pseudogene of this gene has been identified on chromosome 9. [provided by RefSeq, Oct 2016]