GPR116 (ADGRF5) (NM_001098518) Human Tagged ORF Clone

CAT#: RG221417

GPR116 (GFP-tagged) - Human G protein-coupled receptor 116 (GPR116), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ADGRF5
• Synonyms: GPR116; KPG_001
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221417 representing NM_001098518
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAATCCCCAAGGAGAACCACTTTGTGCCTCATGTTTATTGTGATTTATTCTTCCAAAGCTGCACTGA
  ACTGGAATTACGAGTCTACTATTCATCCTTTGAGTCTTCATGAACATGAACCAGCTGGTGAAGAGGCACT
  GAGGCAAAAACGAGCCGTTGCCACAAAAAGTCCTACGGCTGAAGAATACACTGTTAATATTGAGATCAGT
  TTTGAAAATGCATCCTTCCTGGATCCTATCAAAGCCTACTTGAACAGCCTCAGTTTTCCAATTCATGGGA
  ATAACACTGACCAAATTACCGACATTTTGAGCATAAATGTGACAACAGTCTGCAGACCTGCTGGAAATGA
  AATCTGGTGCTCCTGCGAGACAGGTTATGGGTGGCCTCGGGAAAGGTGTCTTCACAATCTCATTTGTCAA
  GAGCGTGACGTCTTCCTCCCAGGGCACCATTGCAGTTGCCTTAAAGAACTGCCTCCCAATGGACCTTTTT
  GCCTGCTTCAGGAAGATGTTACCCTGAACATGAGAGTCAGACTAAATGTAGGCTTTCAAGAAGACCTCAT
  GAACACTTCCTCCGCCCTCTATAGGTCCTACAAGACCGACTTGGAAACAGCGTTCCGGAAGGGTTACGGA
  ATTTTACCAGGCTTCAAGGGCGTGACTGTGACAGGGTTCAAGTCTGGAAGTGTGGTTGTGACATATGAAG
  TCAAGACTACACCACCATCACTTGAGTTAATACATAAAGCCAATGAACAAGTTGTACAGAGCCTCAATCA
  GACCTACAAAATGGACTACAACTCCTTTCAAGCAGTTACTATCAATGAAAGCAATTTCTTTGTCACACCA
  GAAATCATCTTTGAAGGGGACACAGTCAGTCTGGTGTGTGAAAAGGAAGTTTTGTCCTCCAATGTGTCTT
  GGCGCTATGAAGAACAGCAGTTGGAAATCCAGAACAGCAGCAGATTCTCGATTTACACCGCACTTTTCAA
  CAACATGACTTCGGTGTCCAAGCTCACCATCCACAACATCACTCCAGGTGATGCAGGTGAATATGTTTGC
  AAACTGATATTAGACATTTTTGAATATGAGTGCAAGAAGAAAATAGATGTTATGCCCATCCAAATTTTGG
  CAAATGAAGAAATGAAGGTGATGTGCGACAACAATCCTGTATCTTTGAACTGCTGCAGTCAGGGTAATGT
  TAATTGGAGCAAAGTAGAATGGAAGCAGGAAGGAAAAATAAATATTCCAGGAACCCCTGAGACAGACATA
  GATTCTAGCTGCAGCAGATACACCCTCAAGGCTGATGGAACCCAGTGCCCAAGCGGGTCGTCTGGAACAA
  CAGTCATCTACACTTGTGAGTTCATCAGTGCCTATGGAGCCAGAGGCAGTGCAAACATAAAAGTGACATT
  CATCTCTGTGGCCAATCTAACAATAACCCCGGACCCAATTTCTGTTTCTGAGGGACAAAACTTTTCTATA
  AAATGCATCAGTGATGTGAGTAACTATGATGAGGTTTATTGGAACACTTCTGCTGGAATTAAAATATACC
  AAAGATTTTATACCACGAGGAGGTATCTTGATGGAGCAGAATCAGTACTGACAGTCAAGACCTCGACCAG
  GGAGTGGAATGGAACCTATCACTGCATATTTAGATATAAGAATTCATACAGTATTGCAACCAAAGACGTC
  ATTGTTCACCCGCTGCCTCTAAAGCTGAACATCATGGTTGATCCTTTGGAAGCTACTGTTTCATGCAGTG
  GTTCCCATCACATCAAGTGCTGCATAGAGGAGGATGGAGACTACAAAGTTACTTTCCATACGGGTTCCTC
  ATCCCTTCCTGCTGCAAAAGAAGTTAACAAAAAACAAGTGTGCTACAAACACAATTTCAATGCAAGCTCA
  GTTTCCTGGTGTTCAAAAACTGTTGATGTGTGTTGTCACTTTACCAATGCTGCTAATAATTCAGTCTGGA
  GCCCATCTATGAAGCTGAATCTGGTTCCTGGGGAAAACATCACATGCCAGGATCCCGTAATAGGTGTCGG
  AGAGCCGGGGAAAGTCATCCAGAAGCTATGCCGGTTCTCAAACGTTCCCAGCAGCCCTGAGAGTCCCATT
  GGCGGGACCATCACTTACAAATGTGTAGGCTCCCAGTGGGAGGAGAAGAGAAATGACTGCATCTCTGCCC
  CAATAAACAGTCTGCTCCAGATGGCTAAGGCTTTGATCAAGAGCCCCTCTCAGGATGAGATGCTCCCTAC
  ATACCTGAAGGATCTTTCTATTAGCATAGACAAAGCGGAACATGAAATCAGCTCTTCTCCTGGGAGTCTG
  GGAGCCATTATTAACATCCTTGATCTGCTCTCAACAGTTCCAACCCAAGTAAATTCAGAAATGATGACGC
  ACGTGCTCTCTACGGTTAATGTCATCCTTGGCAAGCCCGTCTTGAACACCTGGAAGGTTTTACAACAGCA
  ATGGACCAATCAGAGTTCACAGCTACTACATTCAGTGGAAAGATTTTCCCAAGCATTACAGTCGGGAGAT
  AGCCCTCCTTTGTCCTTCTCCCAAACTAATGTGCAGATGAGCAGCATGGTAATCAAGTCCAGCCACCCAG
  AAACCTATCAACAGAGGTTTGTTTTCCCATACTTTGACCTCTGGGGCAATGTGGTCATTGACAAGAGCTA
  TCTAGAAAACTTGCAGTCGGATTCGTCTATTGTCACCATGGCTTTCCCAACTCTCCAAGCCATCCTTGCC
  CAGGATATCCAGGAAAATAACTTTGCAGAGAGCTTAGTGATGACAACCACTGTCAGCCACAATACAACTA
  TGCCATTCAGGATTTCAATGACTTTTAAGAACAATAGCCCTTCAGGCGGCGAAACGAAGTGTGTCTTCTG
  GAACTTCAGGCTTGCCAACAACACAGGGGGGTGGGACAGCAGTGGGTGCTATGTAGAAGAAGGTGATGGG
  GACAATGTCACCTGTATCTGTGACCACCTAACATCATTCTCCATCCTCATGTCCCCTGACTCCCCAGATC
  CTAGTTCTCTCCTGGGAATACTCCTGGATATTATTTCTTATGTTGGGGTGGGCTTTTCCATCTTGAGCTT
  GGCAGCCTGTCTAGTTGTGGAAGCTGTGGTGTGGAAATCGGTGACCAAGAACCGGACTTCTTATATGCGC
  CACACCTGCATAGTGAATATCGCTGCCTCCCTTCTGGTCGCCAACACCTGGTTCATTGTGGTCGCTGCCA
  TCCAGGACAATCGCTACATACTCTGCAAGACAGCCTGTGTGGCTGCCACCTTCTTCATCCACTTCTTCTA
  CCTCAGCGTCTTCTTCTGGATGCTGACACTGGGCCTCATGCTGTTCTATCGCCTGGTTTTCATTCTGCAT
  GAAACAAGCAGGTCCACTCAGAAAGCCATTGCCTTCTGTCTTGGCTATGGCTGCCCACTTGCCATCTCGG
  TCATCACGCTGGGAGCCACCCAGCCCCGGGAAGTCTATACGAGGAAGAATGTCTGTTGGCTCAACTGGGA
  GGACACCAAGGCCCTGCTGGCTTTCGCCATCCCAGCACTGATCATTGTGGTGGTGAACATAACCATCACT
  ATTGTGGTCATCACCAAGATCCTGAGGCCTTCCATTGGAGACAAGCCATGCAAGCAGGAGAAGAGCAGCC
  TGTTTCAGATCAGCAAGAGCATTGGGGTCCTCACACCACTCTTGGGCCTCACTTGGGGTTTTGGTCTCAC
  CACTGTGTTCCCAGGGACCAACCTTGTGTTCCATATCATATTTGCCATCCTCAATGTCTTCCAGGGATTA
  TTCATTTTACTCTTTGGATGCCTCTGGGATCTGAAGGTACAGGAAGCTTTGCTGAATAAGTTTTCATTGT
  CGAGATGGTCTTCACAGCACTCAAAGTCAACATCCCTGGGTTCATCCACACCTGTGTTTTCTATGAGTTC
  TCCAATATCAAGGAGATTTAACAATTTGTTTGGTAAAACAGGAACGTATAATGTTTCCACCCCAGAAGCA
  ACCAGCTCATCCCTGGAAAACTCATCCAGTGCTTCTTCGTTGCTCAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221417 representing NM_001098518
  Red=Cloning site Green=Tags(s)
  
  MKSPRRTTLCLMFIVIYSSKAALNWNYESTIHPLSLHEHEPAGEEALRQKRAVATKSPTAEEYTVNIEIS
  FENASFLDPIKAYLNSLSFPIHGNNTDQITDILSINVTTVCRPAGNEIWCSCETGYGWPRERCLHNLICQ
  ERDVFLPGHHCSCLKELPPNGPFCLLQEDVTLNMRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYG
  ILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTP
  EIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGEYVC
  KLILDIFEYECKKKIDVMPIQILANEEMKVMCDNNPVSLNCCSQGNVNWSKVEWKQEGKINIPGTPETDI
  DSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVANLTITPDPISVSEGQNFSI
  KCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDV
  IVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHTGSSSLPAAKEVNKKQVCYKHNFNASS
  VSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPI
  GGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSL
  GAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGD
  SPPLSFSQTNVQMSSMVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILA
  QDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDG
  DNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMR
  HTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILH
  ETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITIT
  IVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGL
  FILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPEA
  TSSSLENSSSASSLLN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001098518
• ORF Size: 4038 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001098518.1, NP_001091988.1
• RefSeq Size: 5822
• RefSeq ORF: 4041
• Locus ID: 221395
• Protein Families: Druggable Genome, GPCR, Transmembrane