DYNC1I2 (NM_001378) Human Tagged ORF Clone

CAT#: RG221434

DYNC1I2 (GFP-tagged) - Human dynein, cytoplasmic 1, intermediate chain 2 (DYNC1I2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DYNC1I2
• Synonyms: DIC74; DNCI2; IC2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221434 representing NM_001378
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGACAAAAGTGAATTAAAGGCTGAGTTGGAACGTAAGAAGCAGCGACTGGCCCAAATCAGAGAGG
  AAAAGAAGAGAAAAGAAGAAGAAAGGAAAAAAAAAGAAACAGACCAGAAGAAGGAAGCTGTTGCTCCTGT
  GCAAGAAGAATCAGATCTTGAAAAAAAAAGGAGAGAAGCTGAAGCATTGCTTCAAAGCATGGGGCTAACT
  CCAGAATCCCCCATTGTTTTTTCTGAATACTGGGTCCCTCCTCCTATGTCTCCATCCTCCAAATCTGTGA
  GCACTCCAAGTGAAGCTGGAAGCCAAGACTCTGGAGATGGCGCCGTGGGATCTAGGACGCTGCATTGGGA
  TACAGATCCATCAGTTCTTCAGCTTCACTCAGATTCCGATTTGGGACGAGGACCTATTAAACTTGGAATG
  GCTAAAATCACGCAAGTCGACTTTCCTCCTCGAGAAATTGTCACGTATACAAAGGAAACTCAGACTCCAG
  TTATGGCTCAACCCAAAGAAGATGAAGAGGAAGATGATGATGTAGTGGCTCCTAAACCACCTATTGAACC
  TGAAGAAGAGAAAACTTTAAAGAAAGATGAGGAAAATGATAGTAAAGCTCCCCCTCATGAGCTGACTGAA
  GAAGAAAAGCAACAAATCCTGCACTCTGAGGAATTTTTAAGTTTCTTTGACCATTCTACAAGAATTGTAG
  AAAGAGCTCTTTCTGAGCAGATTAACATCTTCTTTGACTATAGTGGGAGAGATTTGGAAGACAAAGAAGG
  AGAGATTCAAGCAGGTGCTAAACTGTCATTAAATCGACAATTTTTTGACGAACGTTGGTCAAAGCATCGG
  GTGGTTAGTTGTTTGGATTGGTCATCTCAGTATCCGGAGTTACTCGTGGCTTCCTATAACAACAATGAAG
  ATGCCCCTCATGAGCCTGATGGTGTGGCCCTTGTATGGAATATGAAATACAAAAAAACTACCCCAGAGTA
  TGTGTTTCACTGCCAGTCAGCTGTGATGTCTGCCACATTTGCAAAATTTCATCCAAATCTTGTTGTTGGT
  GGTACATATTCAGGCCAAATTGTGCTTTGGGATAACCGTAGCAATAAAAGAACTCCAGTGCAAAGAACTC
  CACTGTCAGCAGCTGCACACACACACCCTGTATATTGTGTAAATGTTGTTGGAACACAAAATGCTCACAA
  TCTGATTAGCATCTCTACTGATGGAAAAATTTGTTCATGGAGTCTGGACATGCTTTCCCATCCACAGGAT
  AGCATGGAGTTGGTTCATAAACAGTCAAAAGCAGTAGCTGTGACATCTATGTCCTTCCCTGTTGGAGATG
  TCAACAACTTTGTTGTTGGGAGTGAAGAAGGTTCTGTGTACACAGCATGCCGCCATGGCAGCAAAGCTGG
  AATCAGTGAGATGTTTGAGGGGCATCAAGGACCAATCACTGGCATCCATTGTCATGCAGCTGTTGGAGCA
  GTAGACTTCTCACATCTTTTTGTCACTTCATCGTTTGACTGGACAGTAAAGCTTTGGACAACTAAGAATA
  ACAAGCCTTTGTATTCATTTGAAGATAATGCAGACTATGTTTATGATGTTATGTGGTCACCTACCCACCC
  AGCCCTGTTTGCCTGTGTGGATGGCATGGGGAGATTGGATTTATGGAATCTCAATAATGACACAGAGGTA
  CCAACTGCCAGCATTTCTGTGGAGGGTAATCCTGCTCTTAATCGTGTGAGATGGACCCATTCTGGCAGAG
  AGATTGCTGTGGGTGATTCTGAAGGACAGATTGTTATATACGATGTGGGAGAGCAGATTGCTGTTCCCCG
  CAATGATGAATGGGCACGGTTTGGCCGAACACTTGCAGAAATTAATGCAAACCGAGCTGATGCAGAGGAG
  GAAGCAGCTACCCGAATACCTGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221434 representing NM_001378
  Red=Cloning site Green=Tags(s)
  
  MSDKSELKAELERKKQRLAQIREEKKRKEEERKKKETDQKKEAVAPVQEESDLEKKRREAEALLQSMGLT
  PESPIVFSEYWVPPPMSPSSKSVSTPSEAGSQDSGDGAVGSRTLHWDTDPSVLQLHSDSDLGRGPIKLGM
  AKITQVDFPPREIVTYTKETQTPVMAQPKEDEEEDDDVVAPKPPIEPEEEKTLKKDEENDSKAPPHELTE
  EEKQQILHSEEFLSFFDHSTRIVERALSEQINIFFDYSGRDLEDKEGEIQAGAKLSLNRQFFDERWSKHR
  VVSCLDWSSQYPELLVASYNNNEDAPHEPDGVALVWNMKYKKTTPEYVFHCQSAVMSATFAKFHPNLVVG
  GTYSGQIVLWDNRSNKRTPVQRTPLSAAAHTHPVYCVNVVGTQNAHNLISISTDGKICSWSLDMLSHPQD
  SMELVHKQSKAVAVTSMSFPVGDVNNFVVGSEEGSVYTACRHGSKAGISEMFEGHQGPITGIHCHAAVGA
  VDFSHLFVTSSFDWTVKLWTTKNNKPLYSFEDNADYVYDVMWSPTHPALFACVDGMGRLDLWNLNNDTEV
  PTASISVEGNPALNRVRWTHSGREIAVGDSEGQIVIYDVGEQIAVPRNDEWARFGRTLAEINANRADAEE
  EAATRIPA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001378
• ORF Size: 1914 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001378.1, NP_001369.1
• RefSeq Size: 2602 bp
• RefSeq ORF: 1917 bp
• Locus ID: 1781
• Cytogenetics: 2q31.1
• Domains: WD40
• Gene Summary: 'This gene encodes a member of the dynein intermediate chain family. The encoded protein is a non-catalytic component of the cytoplasmic dynein 1 complex, which acts as a retrograde microtubule motor to transport organelles and vesicles. A pseudogene of this gene is located on chromosome 10. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]'