MLH3 (NM_001040108) Human Tagged ORF Clone

CAT#: RG221591

MLH3 (GFP-tagged) - Human mutL homolog 3 (E. coli) (MLH3), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MLH3
• Synonyms: HNPCC7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221591 representing NM_001040108
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCAAGTGCTTGTCAGTTGAAGTACAAGCCAAATTGCGTTCTGGTTTGGCCATAAGCTCCTTGGGCC
  AATGTGTTGAGGAACTTGCCCTCAACAGTATTGATGCTGAAGCAAAATGTGTGGCTGTCAGGGTGAATAT
  GGAAACCTTCCAAGTTCAAGTGATAGACAATGGATTTGGGATGGGGAGTGATGATGTAGAGAAAGTGGGA
  AATCGTTATTTCACCAGTAAATGCCACTCGGTACAGGACTTGGAGAATCCAAGGTTTTATGGTTTCCGAG
  GAGAGGCCTTGGCAAATATTGCTGACATGGCCAGTGCTGTGGAAATTTCGTCCAAGAAAAACAGGACAAT
  GAAAACTTTTGTGAAACTGTTTCAGAGTGGAAAAGCCCTGAAAGCTTGTGAAGCTGATGTGACTAGAGCA
  AGCGCTGGGACTACTGTAACAGTGTATAACCTATTTTACCAGCTTCCTGTAAGGAGGAAATGCATGGACC
  CTAGACTGGAGTTTGAGAAGGTTAGGCAGAGAATAGAAGCTCTCTCACTCATGCACCCTTCCATTTCTTT
  CTCTTTGAGAAATGATGTTTCTGGTTCCATGGTTCTTCAGCTCCCTAAAACCAAAGACGTATGTTCCCGA
  TTTTGTCAAATTTATGGATTGGGAAAGTCCCAAAAGCTAAGAGAAATAAGTTTTAAATATAAAGAGTTTG
  AGCTTAGTGGCTATATCAGCTCTGAAGCACATTACAACAAGAATATGCAGTTTTTGTTTGTGAACAAAAG
  ACTAGTTTTAAGGACAAAGCTACATAAACTCATTGACTTTTTATTAAGGAAAGAAAGTATTATATGCAAG
  CCAAAGAATGGTCCCACCAGTAGGCAAATGAATTCAAGTCTTCGGCACCGGTCTACCCCAGAACTCTATG
  GCATATATGTAATTAATGTGCAGTGCCAATTCTGTGAGTATGATGTGTGCATGGAGCCAGCCAAAACTCT
  GATTGAATTTCAGAACTGGGACACTCTCTTGTTTTGCATTCAGGAAGGAGTGAAAATGTTTTTAAAGCAA
  GAAAAATTATTTGTGGAATTATCAGGTGAGGATATTAAGGAATTTAGTGAAGATAATGGTTTTAGTTTAT
  TTGATGCTACTCTTCAGAAGCGTGTGACTTCCGATGAGAGGAGCAATTTCCAGGAAGCATGTAATAATAT
  TTTAGATTCCTATGAGATGTTTAATTTGCAGTCAAAAGCTGTGAAAAGAAAAACTACTGCAGAAAACGTA
  AACACACAGAGTTCTAGGGATTCAGAAGCTACCAGAAAAAATACAAATGATGCATTTTTGTACATTTATG
  AATCAGGTGGTCCAGGCCATAGCAAAATGACAGAGCCATCTTTACAAAACAAAGACAGCTCTTGCTCAGA
  ATCAAAGATGTTAGAACAAGAGACAATTGTAGCATCAGAAGCTGGAGAAAATGAGAAACATAAAAAATCT
  TTCCTGGAACATAGCTCTTTAGAAAATCCGTGTGGAACCAGTTTAGAAATGTTTTTAAGCCCTTTTCAGA
  CACCATGTCACTTTGAGGAGAGTGGGCAGGATCTAGAAATATGGAAAGAAAGTACTACTGTTAATGGCAT
  GGCTGCCAACATCTTGAAAAATAATAGAATTCAGAATCAACCAAAGAGATTTAAAGATGCTACTGAAGTG
  GGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGAGTACATAGTGCTCAGACAGAGAAAGAGAAAA
  AAAAAGAATCTAGCAATTGTGGAAGAAGAAATGTTTTTAGTTATGGGCGAGTTAAATTATGTTCCACTGG
  CTTTATAACTCATGTAGTACAAAATGAAAAAACTAAATCAACTGAAACAGAACATTCATTTAAAAATTAT
  GTTAGACCTGGTCCCACACGTGCCCAAGAAACATTTGGAAATAGAACACGTCATTCAGTTGAAACTCCAG
  ACATCAAAGATTTAGCCAGCACTTTAAGTAAAGAATCTGGTCAATTGCCCAACAAAAAAAATTGCAGAAC
  GAATATAAGTTATGGGCTAGAGAATGAACCTACAGCAACTTATACAATGTTTTCTGCTTTTCAGGAAGGT
  AGCAAAAAATCACAAACAGATTGCATATTATCTGATACATCCCCCTCTTTCCCCTGGTATAGACACGTTT
  CCAATGATAGTAGGAAAACAGATAAATTAATTGGTTTCTCCAAACCAATCGTCCGTAAGAAGCTAAGCTT
  GAGTTCACAGCTAGGATCTTTAGAGAAGTTTAAGAGGCAATATGGGAAGGTTGAAAATCCTCTGGATACA
  GAAGTAGAGGAAAGTAATGGAGTCACTACCAATCTCAGTCTTCAAGTTGAACCTGACATTCTGCTGAAGG
  ACAAGAACCGCTTAGAGAACTCTGATGTTTGTAAAATCACTACTATGGAGCATAGTGATTCAGATAGTAG
  TTGTCAACCAGCAAGCCACATCCTTAACTCAGAGAAGTTTCCATTCTCCAAGGATGAAGATTGTTTAGAA
  CAACAGATGCCTAGTTTGAGAGAAAGTCCTATGACCCTGAAGGAGTTATCTCTCTTTAATAGAAAACCTT
  TGGACCTTGAGAAGTCATCTGAATCACTAGCCTCTAAATTATCCAGACTGAAGGGTTCCGAAAGAGAAAC
  TCAAACAATGGGGATGATGAGTCGTTTTAATGAACTTCCAAATTCAGATTCCAGTAGGAAAGACAGCAAG
  TTGTGCAGTGTGTTAACACAAGATTTTTGTATGTTATTTAACAACAAGCATGAAAAAACAGAGAATGGTG
  TCATCCCAACATCAGATTCTGCCACACAGGATAATTCCTTTAATAAAAATAGTAAAACACATTCTAACAG
  CAATACAACAGAGAACTGTGTGATATCAGAAACTCCTTTGGTATTGCCCTATAATAATTCTAAAGTTACC
  GGTAAAGATTCAGATGTTCTTATCAGAGCCTCAGAACAACAGATAGGAAGTCTTGACTCTCCCAGTGGAA
  TGTTAATGAATCCGGTAGAAGATGCCACAGGTGACCAAAATGGAATTTGTTTTCAGAGTGAGGAATCTAA
  AGCAAGAGCTTGTTCTGAAACTGAAGAGTCAAACACGTGTTGTTCAGATTGGCAGCGGCATTTCGATGTA
  GCCCTGGGAAGAATGGTTTATGTCAACAAAATGACTGGACTCAGCACATTCATTGCCCCAACTGAGGACA
  TTCAGGCTGCTTGTACTAAAGACCTGACAACTGTGGCTGTGGATGTTGTACTTGAGAATGGGTCTCAGTA
  CAGGTGTCAACCTTTTAGAAGCGACCTTGTTCTTCCTTTCCTTCCGAGAGCTCGAGCAGAGAGGACTGTG
  ATGAGACAGGATAACAGAGATACTGTGGATGATACTGTTAGTAGCGAATCGCTTCAGTCTTTGTTCTCAG
  AATGGGACAATCCAGTATTTGCCCGTTATCCAGAGGTTGCTGTTGATGTAAGCAGTGGCCAGGCTGAGAG
  CTTAGCAGTTAAAATTCACAACATCTTGTATCCCTATCGTTTCACCAAAGGAATGATTCATTCAATGCAG
  GTTCTCCAGCAAGTAGATAACAAGTTTATTGCCTGTTTGATGAGCACTAAGACTGAAGAGAATGGCGAGG
  CAGGTGGGAACCTGCTCGTGCTGGTGGATCAGCACGCTGCCCATGAGCGTATACGTCTGGAGCAGCTTAT
  CATTGATTCCTACGAGAAGCAACAGGCACAAGGCTCTGGTCGGAAAAAATTACTGTCTTCTACTCTAATT
  CCTCCGCTAGAGATAACAGTGACAGAGGAACAAAGGAGACTCTTATGGTGTTACCACAAAAATCTGGAAG
  ATCTGGGCCTTGAATTTGTATTTCCAGACACTAGTGATTCTCTGGTCCTTGTGGGAAAAGTACCACTATG
  TTTTGTGGAAAGAGAAGCCAATGAACTTCGGAGAGGAAGATCTACTGTGACCAAGAGTATTGTGGAGGAA
  TTTATCCGAGAACAACTGGAGCTACTCCAGACCACCGGAGGCATCCAAGGGACATTGCCACTGACTGTCC
  AGAAGGTGTTGGCATCCCAAGCCTGCCATGGGGCCATTAAGTTTAATGATGGCCTGAGCTTACAGGAAAG
  TTGCCGCCTTATTGAAGCTCTGTCCTCATGCCAGCTGCCATTCCAGTGTGCTCACGGGAGACCTTCTATG
  CTGCCGTTAGCTGACATAGACCACTTGGAACAGGAAAAACAGATTAAACCCAACCTCACTAAACTTCGCA
  AAATGGCCCAGGCCTGGCGTCTCTTTGGAAAAGCAGAGTGTGATACAAGGCAGAGCCTGCAGCAATCCAT
  GCCTCCCTGTGAGCCACCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221591 representing NM_001040108
  Red=Cloning site Green=Tags(s)
  
  MIKCLSVEVQAKLRSGLAISSLGQCVEELALNSIDAEAKCVAVRVNMETFQVQVIDNGFGMGSDDVEKVG
  NRYFTSKCHSVQDLENPRFYGFRGEALANIADMASAVEISSKKNRTMKTFVKLFQSGKALKACEADVTRA
  SAGTTVTVYNLFYQLPVRRKCMDPRLEFEKVRQRIEALSLMHPSISFSLRNDVSGSMVLQLPKTKDVCSR
  FCQIYGLGKSQKLREISFKYKEFELSGYISSEAHYNKNMQFLFVNKRLVLRTKLHKLIDFLLRKESIICK
  PKNGPTSRQMNSSLRHRSTPELYGIYVINVQCQFCEYDVCMEPAKTLIEFQNWDTLLFCIQEGVKMFLKQ
  EKLFVELSGEDIKEFSEDNGFSLFDATLQKRVTSDERSNFQEACNNILDSYEMFNLQSKAVKRKTTAENV
  NTQSSRDSEATRKNTNDAFLYIYESGGPGHSKMTEPSLQNKDSSCSESKMLEQETIVASEAGENEKHKKS
  FLEHSSLENPCGTSLEMFLSPFQTPCHFEESGQDLEIWKESTTVNGMAANILKNNRIQNQPKRFKDATEV
  GCQPLPFATTLWGVHSAQTEKEKKKESSNCGRRNVFSYGRVKLCSTGFITHVVQNEKTKSTETEHSFKNY
  VRPGPTRAQETFGNRTRHSVETPDIKDLASTLSKESGQLPNKKNCRTNISYGLENEPTATYTMFSAFQEG
  SKKSQTDCILSDTSPSFPWYRHVSNDSRKTDKLIGFSKPIVRKKLSLSSQLGSLEKFKRQYGKVENPLDT
  EVEESNGVTTNLSLQVEPDILLKDKNRLENSDVCKITTMEHSDSDSSCQPASHILNSEKFPFSKDEDCLE
  QQMPSLRESPMTLKELSLFNRKPLDLEKSSESLASKLSRLKGSERETQTMGMMSRFNELPNSDSSRKDSK
  LCSVLTQDFCMLFNNKHEKTENGVIPTSDSATQDNSFNKNSKTHSNSNTTENCVISETPLVLPYNNSKVT
  GKDSDVLIRASEQQIGSLDSPSGMLMNPVEDATGDQNGICFQSEESKARACSETEESNTCCSDWQRHFDV
  ALGRMVYVNKMTGLSTFIAPTEDIQAACTKDLTTVAVDVVLENGSQYRCQPFRSDLVLPFLPRARAERTV
  MRQDNRDTVDDTVSSESLQSLFSEWDNPVFARYPEVAVDVSSGQAESLAVKIHNILYPYRFTKGMIHSMQ
  VLQQVDNKFIACLMSTKTEENGEAGGNLLVLVDQHAAHERIRLEQLIIDSYEKQQAQGSGRKKLLSSTLI
  PPLEITVTEEQRRLLWCYHKNLEDLGLEFVFPDTSDSLVLVGKVPLCFVEREANELRRGRSTVTKSIVEE
  FIREQLELLQTTGGIQGTLPLTVQKVLASQACHGAIKFNDGLSLQESCRLIEALSSCQLPFQCAHGRPSM
  LPLADIDHLEQEKQIKPNLTKLRKMAQAWRLFGKAECDTRQSLQQSMPPCEPP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001040108
• ORF Size: 4359 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001040108.1, NP_001035197.1
• RefSeq Size: 7911
• RefSeq ORF: 4362
• Locus ID: 27030
• Protein Families: Druggable Genome
• Protein Pathways: Mismatch repair
• Gene Summary: This gene is a member of the MutL-homolog (MLH) family of DNA mismatch repair (MMR) genes. MLH genes are implicated in maintaining genomic integrity during DNA replication and after meiotic recombination. The protein encoded by this gene functions as a heterodimer with other family members. Somatic mutations in this gene frequently occur in tumors exhibiting microsatellite instability, and germline mutations have been linked to hereditary nonpolyposis colorectal cancer type 7 (HNPCC7). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008]