NY BR 1 (ANKRD30A) (NM_052997) Human Tagged ORF Clone

CAT#: RG221917

ANKRD30A (GFP-tagged) - Human ankyrin repeat domain 30A (ANKRD30A)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ANKRD30A
• Synonyms: NY-BR-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221917 representing NM_052997
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAAAGAGGAAGAAGACCATCAACCTTAATATACAAGACGCCCAGAAGAGGACTGCTCTACACTGGG
  CCTGTGTCAATGGCCATGAGGAAGTAGTAACATTTCTGGTAGACAGAAAGTGCCAGCTTGACGTCCTTGA
  TGGCGAACACAGGACACCTCTGATGAAGGCTCTACAATGCCATCAGGAGGCTTGTGCAAATATTCTGATA
  GATTCTGGTGCCGATATAAATCTCGTAGATGTGTATGGCAACATGGCTCTCCATTATGCTGTTTATAGTG
  AGATTTTGTCAGTGGTGGCAAAACTGCTGTCCCATGGTGCAGTCATCGAAGTGCACAACAAGGCTAGCCT
  CACACCACTTTTACTATCCATAACGAAAAGAAGTGAGCAAATTGTGGAATTTTTGCTGATAAAAAATGCA
  AATGCGAATGCAGTTAATAAGTATAAATGCACAGCCCTCATGCTTGCTGTATGTCATGGATCATCAGAGA
  TAGTTGGCATGCTTCTTCAGCAAAATGTTGACGTCTTTGCTGCAGATATATGTGGAGTAACTGCAGAACA
  TTATGCTGTTACTTGTGGATTTCATCACATTCATGAACAAATTATGGAATATATACGAAAATTATCTAAA
  AATCATCAAAATACCAATCCAGAAGGAACATCTGCAGGAACACCTGATGAGGCTGCACCCTTGGCGGAAA
  GAACACCTGACACAGCTGAAAGCTTGGTGGAAAAAACACCTGATGAGGCTGCACCCTTGGTGGAAAGAAC
  ACCTGACACGGCTGAAAGCTTGGTGGAAAAAACACCTGATGAGGCTGCATCCTTGGTGGAGGGAACATCT
  GACAAAATTCAATGTTTGGAGAAAGCGACATCTGGAAAGTTCGAACAGTCAGCAGAAGAAACACCTAGGG
  AAATTACGAGTCCTGCAAAAGAAACATCTGAGAAATTTACGTGGCCAGCAAAAGGAAGACCTAGGAAGAT
  CGCATGGGAGAAAAAAGAAGACACACCTAGGGAAATTATGAGTCCCGCAAAAGAAACATCTGAGAAATTT
  ACGTGGGCAGCAAAAGGAAGACCTAGGAAGATCGCATGGGAGAAAAAAGAAACACCTGTAAAGACTGGAT
  GCGTGGCAAGAGTAACATCTAATAAAACTAAAGTTTTGGAAAAAGGAAGATCTAAGATGATTGCATGTCC
  TACAAAAGAATCATCTACAAAAGCAAGTGCCAATGATCAGAGGTTCCCATCAGAATCCAAACAAGAGGAA
  GATGAAGAATATTCTTGTGATTCTCGGAGTCTCTTTGAGAGTTCTGCAAAGATTCAAGTGTGTATACCTG
  AGTCTATATATCAAAAAGTAATGGAGATAAATAGAGAAGTAGAAGAGCCTCCTAAGAAGCCATCTGCCTT
  CAAGCCTGCCATTGAAATGCAAAACTCTGTTCCAAATAAAGCCTTTGAATTGAAGAATGAACAAACATTG
  AGAGCAGATCCGATGTTCCCACCAGAATCCAAACAAAAGGACTATGAAGAAAATTCTTGGGATTCTGAGA
  GTCTCTGTGAGACTGTTTCACAGAAGGATGTGTGTTTACCCAAGGCTACACATCAAAAAGAAATAGATAA
  AATAAATGGAAAATTAGAAGAGTCTCCTAATAAAGATGGTCTTCTGAAGGCTACCTGCGGAATGAAAGTT
  TCTATTCCAACTAAAGCCTTAGAATTGAAGGACATGCAAACTTTCAAAGCGGAGCCTCCGGGGAAGCCAT
  CTGCCTTCGAGCCTGCCACTGAAATGCAAAAGTCTGTCCCAAATAAAGCCTTGGAATTGAAAAATGAACA
  AACATGGAGAGCAGATGAGATACTCCCATCAGAATCCAAACAAAAGGACTATGAAGAAAATTCTTGGGAT
  ACTGAGAGTCTCTGTGAGACTGTTTCACAGAAGGATGTGTGTTTACCCAAGGCTGCGCATCAAAAAGAAA
  TAGATAAAATAAATGGAAAATTAGAAGGGTCTCCTGTTAAAGATGGTCTTCTGAAGGCTAACTGCGGAAT
  GAAAGTTTCTATTCCAACTAAAGCCTTAGAATTGATGGACATGCAAACTTTCAAAGCAGAGCCTCCCGAG
  AAGCCATCTGCCTTCGAGCCTGCCATTGAAATGCAAAAGTCTGTTCCAAATAAAGCCTTGGAATTGAAGA
  ATGAACAAACATTGAGAGCAGATGAGATACTCCCATCAGAATCCAAACAAAAGGACTATGAAGAAAGTTC
  TTGGGATTCTGAGAGTCTCTGTGAGACTGTTTCACAGAAGGATGTGTGTTTACCCAAGGCTACACATCAA
  AAAGAAATAGATAAAATAAATGGAAAATTAGAAGAGTCTCCTGATAATGATGGTTTTCTGAAGGCTCCCT
  GCAGAATGAAAGTTTCTATTCCAACTAAAGCCTTAGAATTGATGGACATGCAAACTTTCAAAGCAGAGCC
  TCCCGAGAAGCCATCTGCCTTCGAGCCTGCCATTGAAATGCAAAAGTCTGTTCCAAATAAAGCCTTGGAA
  TTGAAGAATGAACAAACATTGAGAGCAGATCAGATGTTCCCTTCAGAATCAAAACAAAAGAAGGTTGAAG
  AAAATTCTTGGGATTCTGAGAGTCTCCGTGAGACTGTTTCACAGAAGGATGTGTGTGTACCCAAGGCTAC
  ACATCAAAAAGAAATGGATAAAATAAGTGGAAAATTAGAAGATTCAACTAGCCTATCAAAAATCTTGGAT
  ACAGTTCATTCTTGTGAAAGAGCAAGGGAACTTCAAAAAGATCACTGTGAACAACGTACAGGAAAAATGG
  AACAAATGAAAAAGAAGTTTTGTGTACTGAAAAAGAAACTGTCAGAAGCAAAAGAAATAAAATCACAGTT
  AGAGAACCAAAAAGTTAAATGGGAACAAGAGCTCTGCAGTGTGAGATTGACTTTAAACCAAGAAGAAGAG
  AAGAGAAGAAATGCCGATATATTAAATGAAAAAATTAGGGAAGAATTAGGAAGAATCGAAGAGCAGCATA
  GGAAAGAGTTAGAAGTGAAACAACAACTTGAACAGGCTCTCAGAATACAAGATATAGAATTGAAGAGTGT
  AGAAAGTAATTTGAATCAGGTTTCTCACACTCATGAAAATGAAAATTATCTCTTACATGAAAATTGCATG
  TTGAAAAAGGAAATTGCCATGCTAAAACTGGAAATAGCCACACTGAAACACCAATACCAGGAAAAGGAAA
  ATAAATACTTTGAGGACATTAAGATTTTAAAAGAAAAGAATGCTGAACTTCAGATGACCCTAAAACTGAA
  AGAGGAATCATTAACTAAAAGGGCATCTCAATATAGTGGGCAGCTTAAAGTTCTGATAGCTGAGAACACA
  ATGCTCACTTCTAAATTGAAGGAAAAACAAGACAAAGAAATACTAGAGGCAGAAATTGAATCACACCATC
  CTAGACTGGCTTCTGCTGTACAAGACCATGATCAAATTGTGACATCAAGAAAAAGTCAAGAACCTGCTTT
  CCACATTGCAGGAGATGCTTGTTTGCAAAGAAAAATGAATGTTGATGTGAGTAGTACGATATATAACAAT
  GAGGTGCTCCATCAACCACTTTCTGAAGCTCAAAGGAAATCCAAAAGCCTAAAAATTAATCTCAATTATG
  CAGGAGATGCTCTAAGAGAAAATACATTGGTTTCAGAACATGCACAAAGAGACCAACGTGAAACACAGTG
  TCAAATGAAGGAAGCTGAACACATGTATCAAAACGAACAAGATAATGTGAACAAACACACTGAACAGCAG
  GAGTCTCTAGATCAGAAATTATTTCAACTACAAAGCAAAAATATGTGGCTTCAACAGCAATTAGTTCATG
  CACATAAGAAAGCTGACAACAAAAGCAAGATAACAATTGATATTCATTTTCTTGAGAGGAAAATGCAACA
  TCATCTCCTAAAAGAGAAAAATGAGGAGATATTTAATTACAATAACCATTTAAAAAACCGTATATATCAA
  TATGAAAAAGAGAAAGCAGAAACAGAAAACTCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221917 representing NM_052997
  Red=Cloning site Green=Tags(s)
  
  MTKRKKTINLNIQDAQKRTALHWACVNGHEEVVTFLVDRKCQLDVLDGEHRTPLMKALQCHQEACANILI
  DSGADINLVDVYGNMALHYAVYSEILSVVAKLLSHGAVIEVHNKASLTPLLLSITKRSEQIVEFLLIKNA
  NANAVNKYKCTALMLAVCHGSSEIVGMLLQQNVDVFAADICGVTAEHYAVTCGFHHIHEQIMEYIRKLSK
  NHQNTNPEGTSAGTPDEAAPLAERTPDTAESLVEKTPDEAAPLVERTPDTAESLVEKTPDEAASLVEGTS
  DKIQCLEKATSGKFEQSAEETPREITSPAKETSEKFTWPAKGRPRKIAWEKKEDTPREIMSPAKETSEKF
  TWAAKGRPRKIAWEKKETPVKTGCVARVTSNKTKVLEKGRSKMIACPTKESSTKASANDQRFPSESKQEE
  DEEYSCDSRSLFESSAKIQVCIPESIYQKVMEINREVEEPPKKPSAFKPAIEMQNSVPNKAFELKNEQTL
  RADPMFPPESKQKDYEENSWDSESLCETVSQKDVCLPKATHQKEIDKINGKLEESPNKDGLLKATCGMKV
  SIPTKALELKDMQTFKAEPPGKPSAFEPATEMQKSVPNKALELKNEQTWRADEILPSESKQKDYEENSWD
  TESLCETVSQKDVCLPKAAHQKEIDKINGKLEGSPVKDGLLKANCGMKVSIPTKALELMDMQTFKAEPPE
  KPSAFEPAIEMQKSVPNKALELKNEQTLRADEILPSESKQKDYEESSWDSESLCETVSQKDVCLPKATHQ
  KEIDKINGKLEESPDNDGFLKAPCRMKVSIPTKALELMDMQTFKAEPPEKPSAFEPAIEMQKSVPNKALE
  LKNEQTLRADQMFPSESKQKKVEENSWDSESLRETVSQKDVCVPKATHQKEMDKISGKLEDSTSLSKILD
  TVHSCERARELQKDHCEQRTGKMEQMKKKFCVLKKKLSEAKEIKSQLENQKVKWEQELCSVRLTLNQEEE
  KRRNADILNEKIREELGRIEEQHRKELEVKQQLEQALRIQDIELKSVESNLNQVSHTHENENYLLHENCM
  LKKEIAMLKLEIATLKHQYQEKENKYFEDIKILKEKNAELQMTLKLKEESLTKRASQYSGQLKVLIAENT
  MLTSKLKEKQDKEILEAEIESHHPRLASAVQDHDQIVTSRKSQEPAFHIAGDACLQRKMNVDVSSTIYNN
  EVLHQPLSEAQRKSKSLKINLNYAGDALRENTLVSEHAQRDQRETQCQMKEAEHMYQNEQDNVNKHTEQQ
  ESLDQKLFQLQSKNMWLQQQLVHAHKKADNKSKITIDIHFLERKMQHHLLKEKNEEIFNYNNHLKNRIYQ
  YEKEKAETENS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_052997
• ORF Size: 4023 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_052997.1, NP_443723.1
• RefSeq Size: 4458
• RefSeq ORF: 4026
• Locus ID: 91074
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a DNA-binding transcription factor that is uniquely expressed in mammary epithelium and the testis. Altered expression levels have been associated with breast cancer progression. [provided by RefSeq, Nov 2016]