CORO1B (NM_001018070) Human Tagged ORF Clone

CAT#: RG222047

CORO1B (GFP-tagged) - Human coronin, actin binding protein, 1B (CORO1B), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CORO1B
• Synonyms: CORONIN-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222047 representing NM_001018070
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTTCCGCAAAGTGGTCCGGCAGAGCAAATTCCGGCATGTGTTCGGGCAGCCGGTCAAGAACGACC
  AGTGCTATGAGGACATTCGCGTGTCCCGTGTTACCTGGGACAGCACCTTCTGCGCCGTCAACCCCAAGTT
  CCTGGCGGTGATTGTGGAGGCCAGTGGAGGGGGTGCCTTTCTGGTGCTCCCCCTAAGCAAGACGGGCCGC
  ATTGACAAGGCCTACCCGACGGTGTGTGGGCACACGGGACCTGTCCTGGACATCGACTGGTGTCCTCACA
  ACGACGAAGTCATAGCCAGCGGCTCGGAGGACTGCACGGTCATGGTGTGGCAGATCCCAGAGAACGGGCT
  GACCTCCCCGCTGACAGAGCCGGTGGTGGTACTGGAGGGGCACACCAAGCGAGTGGGCATCATCGCCTGG
  CACCCCACGGCCCGAAACGTGCTGCTCAGTGCAGGCTGCGACAACGTGGTACTCATCTGGAATGTGGGCA
  CAGCGGAGGAGCTGTACCGCCTGGACAGCCTGCACCCTGACCTCATCTACAATGTCAGCTGGAACCACAA
  TGGCAGCCTGTTTTGCTCAGCATGCAAGGACAAGAGCGTGCGCATCATCGACCCCCGTCGGGGCACCCTG
  GTGGCAGAGCGGGAGAAGGCTCATGAGGGGGCCCGGCCCATGCGGGCCATCTTCCTGGCAGATGGCAAGG
  TGTTCACCACAGGCTTCAGCCGAATGAGCGAGCGGCAGCTGGCGCTCTGGGACCCAGAAAACCTCGAGGA
  ACCCATGGCCCTGCAGGAACTGGACTCGAGCAACGGGGCCCTGCTGCCCTTCTACGACCCCGACACCAGT
  GTGGTCTACGTCTGCGGCAAGGGTGACTCCAGCATCCGGTACTTTGAGATCACAGAGGAGCCTCCCTACA
  TCCACTTCCTGAACACGTTCACCAGCAAGGAGCCGCAGCGGGGTATGGGCAGCATGCCCAAGCGGGGCCT
  GGAGGTCAGCAAGTGCGAGATCGCCCGGTTCTACAAACTGCATGAGCGCAAGTGTGAGCCCATCGTCATG
  ACTGTGCCAAGAAAGTCGGACCTCTTCCAGGATGATCTGTACCCCGACACAGCCGGGCCCGAGGCAGCCC
  TGGAGGCTGAGGAGTGGGTGAGCGGGCGGGATGCCGACCCGATCCTCATCTCACTGCGGGAGGCCTACGT
  GCCCAGCAAGCAGCGGGACCTGAAGATCAGCCGGCGCAACGTGTTGTCTGACAGCCGGCCCGCCATGGCC
  CCGGGCTCCTCCCACCTAGGGGCCCCCGCCTCCACCACCACTGCTGCTGATGCCACCCCCAGCGGCAGCC
  TGGCCAGAGCCGGGGAGGCTGGGAAGCTGGAGGAGGTGATGCAGGAGCTGCGGGCCCTGAGGGCGCTGGT
  CAAGGAGCAGGGCGACCGCATCTGCCGCCTGGAGGAGCAGCTGGGCCGCATGGAGAACGGGGATGCG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222047 representing NM_001018070
  Red=Cloning site Green=Tags(s)
  
  MSFRKVVRQSKFRHVFGQPVKNDQCYEDIRVSRVTWDSTFCAVNPKFLAVIVEASGGGAFLVLPLSKTGR
  IDKAYPTVCGHTGPVLDIDWCPHNDEVIASGSEDCTVMVWQIPENGLTSPLTEPVVVLEGHTKRVGIIAW
  HPTARNVLLSAGCDNVVLIWNVGTAEELYRLDSLHPDLIYNVSWNHNGSLFCSACKDKSVRIIDPRRGTL
  VAEREKAHEGARPMRAIFLADGKVFTTGFSRMSERQLALWDPENLEEPMALQELDSSNGALLPFYDPDTS
  VVYVCGKGDSSIRYFEITEEPPYIHFLNTFTSKEPQRGMGSMPKRGLEVSKCEIARFYKLHERKCEPIVM
  TVPRKSDLFQDDLYPDTAGPEAALEAEEWVSGRDADPILISLREAYVPSKQRDLKISRRNVLSDSRPAMA
  PGSSHLGAPASTTTAADATPSGSLARAGEAGKLEEVMQELRALRALVKEQGDRICRLEEQLGRMENGDA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001018070
• ORF Size: 1467 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001018070.1, NP_001018080.1
• RefSeq Size: 1920 bp
• RefSeq ORF: 1470 bp
• Locus ID: 57175
• Cytogenetics: 11q13.2
• Gene Summary: Members of the coronin family, such as CORO1B, are WD repeat-containing actin-binding proteins that regulate cell motility (Cai et al., 2005 [PubMed 16027158]). [supplied by OMIM, Mar 2008]