ALPK1 (NM_001102406) Human Tagged ORF Clone

CAT#: RG222061

ALPK1 (GFP-tagged) - Human alpha-kinase 1 (ALPK1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ALPK1
• Synonyms: 8430410J10Rik; LAK
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222061 representing NM_001102406
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATAATCAAAAAGTGGTAGCTGTGCTACTGCAAGAGTGCAAGCAAGTGCTGGATCAGCTCTTGTTGG
  AAGCGCCAGATGTGTCGGAAGAGGACAAGAGCGAGGACCAGCGCTGCAGAGCTTTACTCCCCAGCGAGTT
  AAGGACCCTGATCCAGGAGGCAAAGGAAATGAAGTGGCCCTTCGTGCCTGAAAAGTGGCAGTACAAACAA
  GCCGTGGGCCCAGAGGACAAAACAAACCTGAAGGATGTGATTGGCGCCGGGTTGCAGCAGTTACTGGCGT
  CCCTGAGGGCCTCCATCCTCGCTCGGGACTGTGCGGCTGCGGCGGCTATTGTGTTCTTGGTGGACCGGTT
  CCTGTATGGGCTCGACGTCTCTGGAAAACTTCTGCAGGTCGCCAAAGGTCTCCACAAGTTGCAGCCAGCC
  ACGCCAATTGCCCCGCAGGTGGTTATTCGCCAAGCCCGAATCTCCGTGAACTCAGGAAAACTTTTAAAAG
  CAGAGTATATTCTGAGCAGTCTAATAAGCAACAATGGAGCAACGGGTACCTGGCTGTACAGAAATGAAAG
  TGACAAGGTCCTGGTGCAGTCGGTCTGTATACAGATCAGAGGGCAGATTCTGCAAAAGCTGGGGATGTGG
  TACGAAGCAGCAGAGTTAATATGGGCCTCCATTGTAGGATATTTGGCACTTCCTCAGCCGGATAAAAAGG
  GCCTCTCCACGTCGCTAGGTATACTGGCAGACATCTTTGTTTCCATGAGCAAGAACGATTATGAAAAGTT
  TAAAAACAATCCACAAATTAATTTGAGCCTGCTGAAGGAGTTTGACCACCATTTGCTGTCCGCTGCAGAA
  GCCTGCAAGCTGGCAGCTGCCTTCAGTGCCTATACGCCGCTCTTCGTGCTCACAGCTGTGAATATCCGTG
  GCACGTGTTTATTGTCCTACAGTAGTTCAAATGACTGTCCTCCAGAATTGAAAAACTTACATCTGTGTGA
  AGCCAAAGAGGCCTTTGAGATTGGCCTCCTCACCAAGAGAGATGATGAGCCTGTTACTGGAAAACAGGAG
  CTTCACAGCTTTGTCAAAGCTGCTTTCGGTCTCACCACAGTGCACAGAAGGCTCCATGGGGAGACAGGGA
  CGGTCCATGCAGCAAGTCAGCTCTGTAAGGAAGCAATGGGGAAGCTGTACAATTTCAGCACTTCCTCCAG
  AAGTCAGGACAGAGAAGCTCTGTCTCAAGAAGTTATGTCTGTGATTGCCCAGGTGAAGGAACATTTACAA
  GTTCAAAGCTTCTCAAATGTAGATGACAGATCTTATGTTCCCGAGAGTTTCGAGTGCAGGTTGGATAAAC
  TTATCTTGCATGGGCAAGGGGATTTCCAAAAAATCCTTGACACCTATTCACAGCACCATACTTCGGTGTG
  TGAAGTATTTGAAAGTGATTGTGGAAACAACAAAAATGAACAGAAAGATGCAAAAACAGGAGTCTGCATC
  ACTGCTCTAAAAACAGAAATAAAAAACATAGATACTGTGAGTACTACTCAAGAAAAGCCACATTGTCAAA
  GAGACACAGGAATATCTTCCTCCCTAATGGGTAAGAATGTTCAGAGGGAACTCAGAAGGGGAGGAAGGAG
  AAACTGGACCCATTCTGATGCATTTCGAGTCTCCTTGGATCAAGATGTGGAGACTGAGACTGAGCCATCG
  GACTACAGCAATGGTGAGGGAGCTGTTTTCAACAAGTCTCTGAGTGGCAGCCAGACTTCCAGTGCTTGGA
  GCAACTTATCAGGGTTTAGTTCCTCTGCAAGCTGGGAGGAAGTGAATTATCACGTTGACGACAGGTCAGC
  CAGAAAAGAGCCTGGCAAAGAACATCTGGTGGACACTCAGTGTTCCACTGCCTTGTCTGAGGAGCTAGAG
  AATGACAGGGAAGGCAGAGCTATGCATTCATTGCATTCACAGCTTCATGATCTCTCTCTTCAGGAACCCA
  ACAATGACAATTTGGAGCCTTCTCAAAATCAGCCACAGCAACAGATGCCCTTGACACCCTTCTCGCCTCA
  TAATACCCCAGGCATTTTCTTGGCCCCTGGTGCAGGGCTTCTAGAAGGAGCTCCAGAAGGTATCCAGGAA
  GTCAGAAATATGGGACCCAGAAATACTTCTGCTCACTCCAGACCCTCATATCGTTCTGCTTCTTGGTCTT
  CTGATTCTGGTAGGCCCAAGAATATGGGCACACATCCTTCAGTCCAAAAAGAAGAAGCCTTTGAAATAAT
  TGTTGAGTTTCCAGAAACCAACTGCGATGTCAAAGACAGGCAGGGGAAAGAGCAGGGAGAAGAAATTAGT
  GAAAGAGGCGCAGGCCCTACATTTAAAGCTAGTCCCTCCTGGGTTGACCCAGAAGGAGAAACAGCAGAAA
  GCACTGAAGATGCACCCTTAGACTTTCACAGGGTCCTGCACAATTCTCTGGGAAACATTTCCATGCTGCC
  ATGTAGCTCCTTCACCCCTAATTGGCCTGTTCAAAATCCTGACTCCAGAAAAAGTGGTGGCCCAGTCGCA
  GAGCAGGGCATCGACCCTGATGCCTCCACAGTGGATGAGGAGGGGCAACTGCTCGACAGCATGGATGTTC
  CCTGCACAAATGGGCACGGCTCTCATAGACTGTGCATTCTGAGACAGCCGCCTGGTCAGAGGGCGGAGAC
  CCCCAATTCCTCTGTAAGCGGTAACATCCTCTTCCCTGTCCTCAGCGAGGACTGCACTACCACAGAGGAA
  GGAAATCAGCCTGGAAACATGCTAAACTGCAGCCAGAACTCCAGCTCATCCTCAGTGTGGTGGCTGAAAT
  CACCTGCATTTTCCAGTGGTTCTTCTGAGGGGGACAGCCCTTGGTCCTATCTGAATTCCAGTGGGAGTTC
  TTGGGTTTCATTGCCGGGAAAGATGAGGAAAGAGATCCTTGAGGCTCGCACCTTGCAACCTGATGACTTT
  GAAAAGCTGTTGGCAGGAGTGAGGCATGATTGGCTGTTTCAGAGACTAGAGAATACGGGGGTTTTTAAGC
  CCAGTCAACTCCACCGAGCACATAGTGCTCTTTTGTTAAAATATTCAAAAAAATCTGAACTGTGGACGGC
  CCAGGAAACTATTGTCTATTTGGGGGACTACTTGACTGTGAAGAAAAAAGGCAGACAAAGAAATGCTTTT
  TGGGTTCATCATCTTCATCAAGAAGAAATTCTGGGGAGGTATGTTGGGAAAGACTATAAGGAGCAGAAGG
  GGCTCTGGCACCACTTCACTGATGTGGAGCGACAGATGACCGCACAGCACTATGTGACAGAATTTAACAA
  GAGACTCTATGAACAAAACATTCCCACCCAGATATTCTACATCCCATCCACAATACTACTGATTTTAGAG
  GACAAGACAATAAAGGGATGTATCAGTGTGGAGCCTTACATACTGGGAGAATTTGTAAAATTGTCAAATA
  ACACGAAAGTGGTGAAAACAGAATACAAAGCCACAGAATATGGCTTGGCCTATGGCCATTTTTCTTATGA
  GTTTTCTAATCATAGAGATGTTGTGGTCGATTTACAAGGTTGGGTAACCGGTAATGGAAAAGGACTCATC
  TACCTCACAGATCCCCAGATTCACTCCGTTGATCAGAAAGTTTTCACTACCAATTTTGGAAAGAGAGGAA
  TTTTTTACTTCTTTAATAACCAGCATGTGGAATGTAATGAAATCTGCCATCGTCTTTCTTTGACTAGACC
  TTCAATGGAGAAACCATGCACA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222061 representing NM_001102406
  Red=Cloning site Green=Tags(s)
  
  MNNQKVVAVLLQECKQVLDQLLLEAPDVSEEDKSEDQRCRALLPSELRTLIQEAKEMKWPFVPEKWQYKQ
  AVGPEDKTNLKDVIGAGLQQLLASLRASILARDCAAAAAIVFLVDRFLYGLDVSGKLLQVAKGLHKLQPA
  TPIAPQVVIRQARISVNSGKLLKAEYILSSLISNNGATGTWLYRNESDKVLVQSVCIQIRGQILQKLGMW
  YEAAELIWASIVGYLALPQPDKKGLSTSLGILADIFVSMSKNDYEKFKNNPQINLSLLKEFDHHLLSAAE
  ACKLAAAFSAYTPLFVLTAVNIRGTCLLSYSSSNDCPPELKNLHLCEAKEAFEIGLLTKRDDEPVTGKQE
  LHSFVKAAFGLTTVHRRLHGETGTVHAASQLCKEAMGKLYNFSTSSRSQDREALSQEVMSVIAQVKEHLQ
  VQSFSNVDDRSYVPESFECRLDKLILHGQGDFQKILDTYSQHHTSVCEVFESDCGNNKNEQKDAKTGVCI
  TALKTEIKNIDTVSTTQEKPHCQRDTGISSSLMGKNVQRELRRGGRRNWTHSDAFRVSLDQDVETETEPS
  DYSNGEGAVFNKSLSGSQTSSAWSNLSGFSSSASWEEVNYHVDDRSARKEPGKEHLVDTQCSTALSEELE
  NDREGRAMHSLHSQLHDLSLQEPNNDNLEPSQNQPQQQMPLTPFSPHNTPGIFLAPGAGLLEGAPEGIQE
  VRNMGPRNTSAHSRPSYRSASWSSDSGRPKNMGTHPSVQKEEAFEIIVEFPETNCDVKDRQGKEQGEEIS
  ERGAGPTFKASPSWVDPEGETAESTEDAPLDFHRVLHNSLGNISMLPCSSFTPNWPVQNPDSRKSGGPVA
  EQGIDPDASTVDEEGQLLDSMDVPCTNGHGSHRLCILRQPPGQRAETPNSSVSGNILFPVLSEDCTTTEE
  GNQPGNMLNCSQNSSSSSVWWLKSPAFSSGSSEGDSPWSYLNSSGSSWVSLPGKMRKEILEARTLQPDDF
  EKLLAGVRHDWLFQRLENTGVFKPSQLHRAHSALLLKYSKKSELWTAQETIVYLGDYLTVKKKGRQRNAF
  WVHHLHQEEILGRYVGKDYKEQKGLWHHFTDVERQMTAQHYVTEFNKRLYEQNIPTQIFYIPSTILLILE
  DKTIKGCISVEPYILGEFVKLSNNTKVVKTEYKATEYGLAYGHFSYEFSNHRDVVVDLQGWVTGNGKGLI
  YLTDPQIHSVDQKVFTTNFGKRGIFYFFNNQHVECNEICHRLSLTRPSMEKPCT
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001102406
• ORF Size: 3732 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001102406.1, NP_001095876.1
• RefSeq Size: 5389
• RefSeq ORF: 3735
• Locus ID: 80216
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes an alpha kinase. Mice which were homozygous for disrupted copies of this gene exhibited coordination defects (PMID: 21208416). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]