THOC2 (NM_001081550) Human Tagged ORF Clone

CAT#: RG222235

THOC2 (GFP-tagged) - Human THO complex 2 (THOC2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: THOC2
• Synonyms: CXorf3; dJ506G2.1; hTREX120; MRX12; MRX35; THO2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222235 representing NM_001081550
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCGCGGCTGTGGTGGTTCCCGCAGAGTGGATAAAGAACTGGGAGAAATCAGGGAGAGGCGAAT
  TTTTGCATTTATGTCGGATCCTCAGTGAAAATAAAAGCCATGATAGTTCAACATACAGAGATTTCCAGCA
  AGCTCTCTATGAGTTGTCATATCATGTAATTAAAGGAAATCTAAAGCATGAACAGGCATCTAATGTTCTT
  AGTGACATTAGTGAATTTCGTGAGGATATGCCCTCCATTCTTGCTGATGTATTCTGCATATTAGACATTG
  AGACAAATTGTTTAGAAGAAAAAAGCAAGAGAGACTATTTTACACAGTTGGTATTAGCATGTTTGTATTT
  AGTTTCAGACACAGTTCTAAAGGAACGCCTGGATCCAGAAACACTGGAATCATTAGGGCTTATCAAACAA
  TCACAGCAATTCAATCAAAAGTCAGTTAAAATCAAGACAAAACTCTTTTATAAGCAGCAAAAATTCAATT
  TGTTAAGAGAAGAGAATGAAGGTTATGCCAAGCTGATTGCTGAATTGGGGCAAGATTTATCTGGAAGTAT
  TACTAGTGATTTAATCTTAGAAAATATCAAATCTTTAATAGGATGCTTTAATCTGGATCCCAATAGAGTT
  TTGGATGTCATTTTAGAAGTGTTTGAATGCAGGCCAGAACACGATGACTTCTTTATATCTTTGTTAGAAT
  CTTACATGAGTATGTGTGAACCGCAAACACTGTGTCATATTCTTGGGTTCAAATTCAAGTTTTACCAGGA
  ACCAAATGGCGAGACACCATCATCTTTATACAGAGTTGCAGCAGTACTTCTACAATTTAATCTTATTGAT
  TTAGATGATCTTTATGTACATCTTCTTCCGGCTGATAATTGCATTATGGATGAACACAAACGAGAAATTG
  CGGAAGCTAAGCAAATTGTTAGAAAGCTTACGATGGTTGTGTTGTCTTCTGAAAAAATGGATGAGCGAGA
  GAAAGAAAAGGAAAAAGAAGAGGAGAAAGTAGAGAAACCACCTGATAACCAAAAACTTGGCTTGTTGGAA
  GCCTTATTAAAGATTGGTGATTGGCAACATGCACAGAACATTATGGATCAGATGCCTCCATACTATGCAG
  CTTCACACAAGCTAATAGCCCTTGCTATTTGCAAGCTCATTCATATAACTATTGAGCCTCTCTACCGAAG
  AGTTGGAGTTCCTAAAGGTGCTAAAGGCTCACCTGTTAATGCTTTGCAAAACAAGAGAGCACCAAAACAA
  GCTGAGAGCTTTGAAGATTTGAGGAGAGACGTGTTCAATATGTTCTGTTACCTTGGTCCTCACCTTTCTC
  ACGATCCCATTTTATTTGCAAAAGTGGTGCGCATAGGCAAGTCATTTATGAAGGAGTTTCAGTCTGATGG
  AAGCAAACAAGAAGATAAAGAAAAAACGGAAGTTATCCTTAGCTGTTTGCTTAGCATTACTGACCAGGTA
  CTACTTCCATCTCTTTCTTTGATGGACTGCAATGCTTGTATGTCTGAGGAACTATGGGGAATGTTTAAAA
  CATTTCCATATCAGCATAGATATCGTCTGTATGGCCAGTGGAAGAATGAAACTTATAACAGTCACCCACT
  TTTAGTAAAAGTTAAAGCTCAAACAATAGACAGAGCCAAATATATCATGAAGCGCCTAACCAAGGAAAAT
  GTGAAGCCTTCTGGAAGACAAATTGGGAAGTTGAGCCACAGCAATCCAACCATTTTGTTTGATTATATCT
  TGTCACAAATACAGAAGTATGATAACTTAATAACACCTGTAGTAGATTCATTGAAATACCTCACTTCACT
  GAATTATGATGTCTTGGCCTATTGTATCATTGAAGCTTTAGCTAATCCAGAAAAGGAAAGAATGAAACAT
  GATGACACAACCATCTCAAGCTGGCTTCAGAGTCTGGCTAGTTTCTGTGGTGCAGTTTTTCGTAAATATC
  CAATTGATCTTGCTGGTCTTCTTCAGTATGTTGCCAATCAGCTAAAGGCGGGCAAAAGTTTTGACCTGCT
  TATATTGAAAGAAGTGGTACAAAAAATGGCAGGAATAGAAATTACAGAGGAAATGACAATGGAGCAACTA
  GAGGCTATGACTGGTGGAGAGCAGCTAAAAGCTGAGGGTGGTTATTTTGGTCAGATCAGAAACACTAAAA
  AATCCTCTCAGAGATTAAAGGATGCTCTATTGGACCATGATCTTGCCCTTCCTCTCTGTCTGCTTATGGC
  TCAGCAGAGAAATGGGGTAATCTTTCAGGAAGGTGGAGAGAAACATTTGAAACTTGTGGGAAAGCTCTAT
  GACCAGTGTCATGATACCCTGGTGCAGTTTGGTGGGTTTTTAGCATCTAATCTGAGCACAGAAGATTATA
  TAAAGCGAGTGCCTTCAATTGATGTACTCTGTAATGAATTTCATACACCCCATGATGCAGCATTTTTCCT
  GTCTAGGCCAATGTATGCCCATCATATTTCGTCAAAGTATGATGAACTTAAAAAATCAGAAAAGGGAAGT
  AAACAGCAACATAAAGTTCATAAGTACATTACATCATGTGAGATGGTGATGGCGCCTGTCCATGAAGCAG
  TGGTCTCCTTACATGTTTCCAAAGTCTGGGATGACATCAGCCCTCAATTCTATGCTACATTCTGGTCATT
  GACAATGTATGACCTTGCAGTTCCACACACCAGCTATGAACGAGAAGTCAATAAACTTAAAGTCCAGATG
  AAAGCAATTGATGACAATCAGGAAATGCCCCCAAATAAAAAGAAAAAAGAGAAGGAGCGCTGTACTGCCC
  TTCAGGACAAGCTTCTTGAAGAAGAAAAGAAACAGATGGAACATGTACAGAGAGTTCTACAGAGATTGAA
  ACTGGAAAAGGACAACTGGCTTTTAGCAAAATCTACCAAAAATGAGACCATCACAAAATTTCTACAGCTG
  TGTATATTTCCTCGATGTATTTTTTCAGCAATTGATGCTGTTTACTGTGCTCGTTTTGTTGAATTGGTAC
  ATCAACAGAAAACTCCAAATTTTTCCACACTTCTTTGCTATGATCGAGTTTTCTCTGACATAATTTACAC
  AGTTGCAAGCTGTACTGAAAATGAAGCCAGTCGATACGGAAGGTTTCTTTGCTGCATGTTAGAGACTGTG
  ACCAGGTGGCATAGTGATAGAGCCACATATGAAAAGGAATGTGGAAACTATCCAGGATTCCTTACCATAT
  TACGGGCAACTGGATTTGATGGTGGAAATAAGGCTGATCAATTAGACTATGAAAATTTTCGACATGTTGT
  ACATAAATGGCATTACAAACTAACCAAGGCATCGGTACATTGCCTTGAAACAGGCGAATATACTCACATC
  AGGAATATCTTGATTGTGCTAACAAAAATACTTCCTTGGTACCCAAAAGTTTTGAATCTGGGTCAAGCTT
  TGGAAAGAAGAGTACACAAAATCTGCCAAGAAGAAAAAGAGAAGAGGCCAGATCTATATGCATTGGCTAT
  GGGCTACTCTGGGCAGTTGAAAAGTAGAAAGTCATACATGATACCTGAAAATGAGTTTCATCACAAAGAC
  CCCCCTCCGAGGAATGCAGTTGCCAGTGTGCAAAATGGGCCTGGTGGTGGGCCTTCTTCATCATCAATAG
  GAAGTGCATCTAAATCGGATGAAAGCAGTACTGAGGAGACTGATAAATCAAGGGAGAGATCTCAGTGTGG
  TGTGAAAGCTGTTAATAAAGCTTCTAGTACCACACCTAAAGGGAATTCAAGCAATGGAAATAGTGGCTCT
  AACAGCAACAAAGCTGTTAAAGAAAATGACAAAGAAAAAGGGAAAGAGAAAGAAAAAGAGAAAAAAGAAA
  AGACTCCAGCTACTACTCCAGAGGCCAGGGTACTTGGTAAAGATGGTAAAGAAAAACCAAAGGAAGAGCG
  GCCAAATAAAGATGAAAAAGCAAGAGAGACCAAGGAAAGAACGCCGAAGTCTGACAAAGAGAAAGAAAAA
  TTCAAGAAGGAAGAAAAAGCTAAAGATGAGAAATTTAAGACCACTGTCCCCAACGCAGAATCAAAATCAA
  CTCAAGAAAGGGAAAGAGAGAAGGAGCCATCCAGAGAAAGAGATATAGCAAAGGAAATGAAATCAAAGGA
  AAATGTTAAAGGAGGAGAAAAAACACCAGTTTCTGGGTCCTTGAAATCACCTGTTCCCAGATCAGATATT
  CCAGAGCCTGAAAGGGAACAAAAACGCCGCAAAATTGATACTCACCCTTCTCCATCACATTCCTCCACAG
  TAAAGGACAGTCTCATCGAACTCAAGGAATCTTCAGCAAAGCTCTACATTAATCATACTCCTCCACCACT
  GTCCAAGAGTAAGGAGAGAGAAATGGACAAGAAAGATTTGGACAAGTCAAGGGAAAGATCCAGAGAAAGA
  GAGAAAAAAGATGAAAAGGACAGGAAAGAGCGGAAAAGGGATCACTCAAACAACGACCGTGAAGTGCCAC
  CGGACTTAACCAAGAGACGTAAAGAGGAGAATGGAACAATGGGGGTTTCAAAACATAAAAGTGAAAGTCC
  TTGTGAATCTCCTTATCCAAATGAGAAAGACAAGGAAAAAAATAAGTCAAAATCTTCAGGCAAAGAAAAA
  GGCAGTGATTCATTTAAATCTGAGAAGATGGATAAAATCTCCTCCGGTGGCAAAAAGGAGTCCAGGCATG
  ATAAAGAAAAGATAGAAAAGAAAGAGAAACGGGACAGTTCAGGAGGAAAGGAAGAGAAGAAACATCATAA
  GTCCTCGGACAAGCACAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222235 representing NM_001081550
  Red=Cloning site Green=Tags(s)
  
  MAAAAVVVPAEWIKNWEKSGRGEFLHLCRILSENKSHDSSTYRDFQQALYELSYHVIKGNLKHEQASNVL
  SDISEFREDMPSILADVFCILDIETNCLEEKSKRDYFTQLVLACLYLVSDTVLKERLDPETLESLGLIKQ
  SQQFNQKSVKIKTKLFYKQQKFNLLREENEGYAKLIAELGQDLSGSITSDLILENIKSLIGCFNLDPNRV
  LDVILEVFECRPEHDDFFISLLESYMSMCEPQTLCHILGFKFKFYQEPNGETPSSLYRVAAVLLQFNLID
  LDDLYVHLLPADNCIMDEHKREIAEAKQIVRKLTMVVLSSEKMDEREKEKEKEEEKVEKPPDNQKLGLLE
  ALLKIGDWQHAQNIMDQMPPYYAASHKLIALAICKLIHITIEPLYRRVGVPKGAKGSPVNALQNKRAPKQ
  AESFEDLRRDVFNMFCYLGPHLSHDPILFAKVVRIGKSFMKEFQSDGSKQEDKEKTEVILSCLLSITDQV
  LLPSLSLMDCNACMSEELWGMFKTFPYQHRYRLYGQWKNETYNSHPLLVKVKAQTIDRAKYIMKRLTKEN
  VKPSGRQIGKLSHSNPTILFDYILSQIQKYDNLITPVVDSLKYLTSLNYDVLAYCIIEALANPEKERMKH
  DDTTISSWLQSLASFCGAVFRKYPIDLAGLLQYVANQLKAGKSFDLLILKEVVQKMAGIEITEEMTMEQL
  EAMTGGEQLKAEGGYFGQIRNTKKSSQRLKDALLDHDLALPLCLLMAQQRNGVIFQEGGEKHLKLVGKLY
  DQCHDTLVQFGGFLASNLSTEDYIKRVPSIDVLCNEFHTPHDAAFFLSRPMYAHHISSKYDELKKSEKGS
  KQQHKVHKYITSCEMVMAPVHEAVVSLHVSKVWDDISPQFYATFWSLTMYDLAVPHTSYEREVNKLKVQM
  KAIDDNQEMPPNKKKKEKERCTALQDKLLEEEKKQMEHVQRVLQRLKLEKDNWLLAKSTKNETITKFLQL
  CIFPRCIFSAIDAVYCARFVELVHQQKTPNFSTLLCYDRVFSDIIYTVASCTENEASRYGRFLCCMLETV
  TRWHSDRATYEKECGNYPGFLTILRATGFDGGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHI
  RNILIVLTKILPWYPKVLNLGQALERRVHKICQEEKEKRPDLYALAMGYSGQLKSRKSYMIPENEFHHKD
  PPPRNAVASVQNGPGGGPSSSSIGSASKSDESSTEETDKSRERSQCGVKAVNKASSTTPKGNSSNGNSGS
  NSNKAVKENDKEKGKEKEKEKKEKTPATTPEARVLGKDGKEKPKEERPNKDEKARETKERTPKSDKEKEK
  FKKEEKAKDEKFKTTVPNAESKSTQEREREKEPSRERDIAKEMKSKENVKGGEKTPVSGSLKSPVPRSDI
  PEPEREQKRRKIDTHPSPSHSSTVKDSLIELKESSAKLYINHTPPPLSKSKEREMDKKDLDKSRERSRER
  EKKDEKDRKERKRDHSNNDREVPPDLTKRRKEENGTMGVSKHKSESPCESPYPNEKDKEKNKSKSSGKEK
  GSDSFKSEKMDKISSGGKKESRHDKEKIEKKEKRDSSGGKEEKKHHKSSDKHR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081550
• ORF Size: 4779 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001081550.1, NP_001075019.1
• RefSeq Size: 5625
• RefSeq ORF: 4782
• Locus ID: 57187
• Protein Pathways: Spliceosome
• Gene Summary: The TREX multiprotein complex binds specifically to spliced mRNAs to facilitate mRNA export. The protein encoded by this gene is a member of the THO complex, a subset of the TREX complex. The encoded protein interacts with the THOC1 protein. [provided by RefSeq, Jun 2010]