ACOX3 (NM_001101667) Human Tagged ORF Clone

CAT#: RG222573

ACOX3 (GFP-tagged) - Human acyl-CoA oxidase 3, pristanoyl (ACOX3), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ACOX3
• Synonyms: acyl-Coenzyme A oxidase 3, pristanoyl
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222573 representing NM_001101667
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCCACTGTGGAAGGAGGCGACACAGCTCTGCTCCCAGAATTCCCCAGGGGGCCCCTCGATGCCT
  ACCGAGCAAGAGCGTCCTTCAGCTGGAAGGAGCTGGCGCTGTTCACGGAAGGGGAGGGCATGCTCCGCTT
  TAAGAAAACCATCTTCTCAGCTCTTGAGAATGACCCTCTTTTCGCTCGTTCCCCTGGAGCCGATCTGTCC
  TTGGAGAAGTATCGCGAGCTGAACTTCCTTCGATGCAAGCGGATCTTCGAGTATGACTTCCTCAGTGTCG
  AAGACATGTTCAAGAGCCCTCTGAAGGTCCCCGCCTTGATTCAGTGCCTGGGCATGTATGACTCTTCTCT
  GGCTGCCAAGTACCTCCTCCATAGCTTGGTTTTTGGATCAGCAGTTTACAGTTCTGGTTCTGAAAGACAT
  CTCACATATATTCAAAAGATCTTCAGGATGGAGATTTTTGGATGTTTTGCTCTGACCGAATTAAGCCACG
  GCAGTAATACCAAGGCCATTCGCACAACTGCCCACTACGATCCTGCCACTGAGGAATTCATCATACATTC
  CCCTGATTTCGAAGCTGCCAAGTTTTGGGTTGGCAACATGGGCAAGACAGCCACTCACGCGGTGGTGTTT
  GCTAAGCTGTGTGTGCCAGGGGACCAGTGCCATGGGCTGCATCCCTTTATCGTGCAGATCCGGGACCCGA
  AGACCCTTCTTCCCATGCCTGGAGTGATGGTTGGCGACATAGGAAAAAAACTCGGGCAGAACGGTCTGGA
  TAATGGTTTCGCCATGTTCCACAAGGTCAGAGTTCCTCGCCAGAGCCTTCTGAACCGGATGGGAGACGTC
  ACCCCCGAGGGCACCTATGTCAGCCCCTTTAAGGACGTCAGGCAGCGCTTTGGAGCGTCCCTGGGGAGCC
  TGTCCTCGGGCCGGGTCTCCATCGTGAGCCTGGCCATCCTTAACCTAAAGCTGGCCGTGGCCATCGCTCT
  TCGCTTCTCAGCCACTCGGCGTCAGTTTGGACCCACAGAGGAGGAGGAAATACCAGTGCTTGAGTATCCA
  ATGCAGCAATGGCGCTTGCTTCCATATCTGGCAGCTGTCTACGCCTTAGACCATTTCTCCAAGTCGCTCT
  TCCTGGACCTGGTGGAGCTCCAGCGAGGACTTGCATCGGGAGACCGCAGCGCCAGACAGGCAGAGCTTGG
  ACGTGAGATCCACGCCCTGGCATCGGCCAGCAAGCCCCTGGCCTCGTGGACCACCCAGCAAGGAATTCAG
  GAATGCCGGGAGGCGTGTGGAGGACACGGCTATCTGGCCATGAACCGGTTGGGTGTCCTTAGAGATGACA
  ACGATCCCAACTGCACATACGAAGGTGACAACAACATCCTGCTGCAGCAGACAAGCAACTATTTGCTGGG
  TCTCCTGGCACACCAGGTCCACGATGGAGCTTGCTTCCGCAGTCCGCTGAAGTCAGTGGACTTTCTGGAC
  GCCTATCCCGGCATCCTTGACCAGAAGTTTGAGGTCTCCAGTGTTGCCGACTGCTTGGACTCTGCAGTCG
  CCCTGGCAGCATACAAGTGGCTGGTTTGCTACCTGCTCCGAGAGACTTATCAAAAATTAAACCAAGAGAA
  AAGATCAGGAAGCAGTGACTTTGAAGCAAGGAACAAATGCCAGGTGTCCCACGGCCGTCCGTTGGCGCTG
  GCCTTCGTGGAGCTCACGGTGGTCCAGAGGTTCCACGAGCACGTGCACCAGCCTTCCGTGCCGCCCTCGC
  TGCGGGCCGTGCTGGGGCGGCTCAGTGCTCTGTACGCCCTGTGGTCCCTGAGCCGCCACGCGGCCCTGCT
  CTACCGAGCTGAAAGACGATGCAGTTGCCCTGGTAGACGTGATCGCTCCTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222573 representing NM_001101667
  Red=Cloning site Green=Tags(s)
  
  MASTVEGGDTALLPEFPRGPLDAYRARASFSWKELALFTEGEGMLRFKKTIFSALENDPLFARSPGADLS
  LEKYRELNFLRCKRIFEYDFLSVEDMFKSPLKVPALIQCLGMYDSSLAAKYLLHSLVFGSAVYSSGSERH
  LTYIQKIFRMEIFGCFALTELSHGSNTKAIRTTAHYDPATEEFIIHSPDFEAAKFWVGNMGKTATHAVVF
  AKLCVPGDQCHGLHPFIVQIRDPKTLLPMPGVMVGDIGKKLGQNGLDNGFAMFHKVRVPRQSLLNRMGDV
  TPEGTYVSPFKDVRQRFGASLGSLSSGRVSIVSLAILNLKLAVAIALRFSATRRQFGPTEEEEIPVLEYP
  MQQWRLLPYLAAVYALDHFSKSLFLDLVELQRGLASGDRSARQAELGREIHALASASKPLASWTTQQGIQ
  ECREACGGHGYLAMNRLGVLRDDNDPNCTYEGDNNILLQQTSNYLLGLLAHQVHDGACFRSPLKSVDFLD
  AYPGILDQKFEVSSVADCLDSAVALAAYKWLVCYLLRETYQKLNQEKRSGSSDFEARNKCQVSHGRPLAL
  AFVELTVVQRFHEHVHQPSVPPSLRAVLGRLSALYALWSLSRHAALLYRAERRCSCPGRRDRSS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001101667
• ORF Size: 1872 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001101667.1, NP_001095137.1
• RefSeq Size: 2822
• RefSeq ORF: 1875
• Locus ID: 8310
• Protein Pathways: alpha-Linolenic acid metabolism, Biosynthesis of unsaturated fatty acids, Fatty acid metabolism, Metabolic pathways, PPAR signaling pathway
• Gene Summary: Acyl-Coenzyme A oxidase 3 also know as pristanoyl -CoA oxidase (ACOX3)is involved in the desaturation of 2-methyl branched fatty acids in peroxisomes. Unlike the rat homolog, the human gene is expressed in very low amounts in liver such that its mRNA was undetectable by routine Northern-blot analysis or its product by immunoblotting or by enzyme activity measurements. However the human cDNA encoding a 700 amino acid protein with a peroxisomal targeting C-terminal tripeptide S-K-L was isolated and is thought to be expressed under special conditions such as specific developmental stages or in a tissue specific manner in tissues that have not yet been examined. [provided by RefSeq, Jul 2008]