PGGT1B (NM_005023) Human Tagged ORF Clone
CAT#: RG222599
- TrueORF®
PGGT1B (GFP-tagged) - Human protein geranylgeranyltransferase type I, beta subunit (PGGT1B)
"NM_005023" in other vectors (6)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | PGGT1B |
Synonyms | BGGI; GGTI |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG222599 representing NM_005023
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGCCACTGAGGATGAGAGGCTAGCAGGGAGCGGTGAGGGAGAGCGGCTGGATTTCTTACGGGATC GGCACGTGCGATTTTTCCAGCGCTGCCTCCAGGTTTTGCCGGAGCGCTATTCTTCACTCGAGACAAGCAG GTTGACAATTGCATTTTTTGCACTCTCCGGGCTGGATATGTTGGATTCCTTAGATGTGGTGAACAAAGAT GATATAATAGAGTGGATTTATTCCCTGCAGGTCCTTCCCACAGAAGACAGATCAAATCTAAATCGCTGTG GTTTCCGAGGCTCTTCATACCTGGGTATTCCGTTCAATCCATCAAAGGCTCCTGGAACAGCTCATCCTTA TGATAGTGGCCACATTGCAATGACCTACACTGGCCTCTCATGCTTAGTTATTCTTGGAGACGACTTAAGC CGAGTAAATAAAGAAGCTTGCTTAGCGGGCTTGAGAGCCCTTCAGCTGGAAGATGGGAGTTTTTGTGCAG TACCTGAAGGCAGTGAAAATGACATGCGATTTGTGTACTGTGCTTCCTGTATTTGCTATATGCTCAACAA CTGGTCAGGCATGGATATGAAAAAAGCCATCACCTATATTAGAAGGAGTATGTCCTATGACAATGGACTG GCACAGGGAGCTGGACTTGAATCTCATGGAGGATCAACTTTTTGTGGCATTGCCTCACTATGTCTGATGG GTAAACTAGAAGAAGTTTTTTCAGAAAAAGAATTGAACAGGATAAAGAGGTGGTGTATAATGAGGCAACA AAATGGTTATCATGGAAGACCTAATAAGCCTGTAGACACCTGTTATTCTTTTTGGGTGGGAGCAACTCTG AAGCTTCTAAAAATTTTCCAATACACTAACTTTGAGAAAAATAGAAATTACATCTTATCAACTCAAGATC GCCTTGTAGGGGGATTTGCCAAGTGGCCAGACAGTCATCCAGATGCTTTGCATGCATACTTTGGGATCTG TGGCCTGTCACTAATGGAGGAAAGTGGAATTTGTAAAGTTCATCCTGCTCTGAATGTAAGCACACGGACT TCTGAACGCCTTCTAGATCTCCATCAAAGCTGGAAAACCAAGGACTCTAAACAATGCTCAGAGAATGTAC ATATCTCCACA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222599 representing NM_005023
Red=Cloning site Green=Tags(s) MVATEDERLAGSGEGERLDFLRDRHVRFFQRCLQVLPERYSSLETSRLTIAFFALSGLDMLDSLDVVNKD DIIEWIYSLQVLPTEDRSNLNRCGFRGSSYLGIPFNPSKAPGTAHPYDSGHIAMTYTGLSCLVILGDDLS RVNKEACLAGLRALQLEDGSFCAVPEGSENDMRFVYCASCICYMLNNWSGMDMKKAITYIRRSMSYDNGL AQGAGLESHGGSTFCGIASLCLMGKLEEVFSEKELNRIKRWCIMRQQNGYHGRPNKPVDTCYSFWVGATL KLLKIFQYTNFEKNRNYILSTQDRLVGGFAKWPDSHPDALHAYFGICGLSLMEESGICKVHPALNVSTRT SERLLDLHQSWKTKDSKQCSENVHIST TRTRPLE - GFP Tag - V |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_005023 |
ORF Size | 1131 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005023.2, NP_005014.1 |
RefSeq Size | 1659 bp |
RefSeq ORF | 1134 bp |
Locus ID | 5229 |
Cytogenetics | 5q22.3 |
Domains | prenyltrans |
Protein Families | Druggable Genome |
Gene Summary | 'Protein geranylgeranyltransferase type I (GGTase-I) transfers a geranylgeranyl group to the cysteine residue of candidate proteins containing a C-terminal CAAX motif in which 'A' is an aliphatic amino acid and 'X' is leucine (summarized by Zhang et al., 1994 [PubMed 8106351]). The enzyme is composed of a 48-kD alpha subunit (FNTA; MIM 134635) and a 43-kD beta subunit, encoded by the PGGT1B gene. The FNTA gene encodes the alpha subunit for both GGTase-I and the related enzyme farnesyltransferase.[supplied by OMIM, Mar 2010]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC313210 | PGGT1B (untagged)-Human protein geranylgeranyltransferase type I, beta subunit (PGGT1B) |
USD 650.00 |
|
RC222599 | PGGT1B (Myc-DDK-tagged)-Human protein geranylgeranyltransferase type I, beta subunit (PGGT1B) |
USD 420.00 |
|
RC222599L1 | Lenti ORF clone of Human protein geranylgeranyltransferase type I, beta subunit (PGGT1B), Myc-DDK-tagged |
USD 620.00 |
|
RC222599L2 | Lenti ORF clone of Human protein geranylgeranyltransferase type I, beta subunit (PGGT1B), mGFP tagged |
USD 620.00 |
|
RC222599L3 | Lenti ORF clone of Human protein geranylgeranyltransferase type I, beta subunit (PGGT1B), Myc-DDK-tagged |
USD 620.00 |
|
RC222599L4 | Lenti ORF clone of Human protein geranylgeranyltransferase type I, beta subunit (PGGT1B), mGFP tagged |
USD 620.00 |
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