PPP2R2B (NM_181678) Human Tagged ORF Clone
CAT#: RG222690
- TrueORF®
PPP2R2B (GFP-tagged) - Human protein phosphatase 2, regulatory subunit B, beta (PPP2R2B), transcript variant 6
"NM_181678" in other vectors (4)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | PPP2R2B |
Synonyms | B55BETA; PP2AB55BETA; PP2ABBETA; PP2APR55B; PP2APR55BETA; PR2AB55BETA; PR2ABBETA; PR2APR55BETA; PR52B; PR55-BETA; PR55BETA; SCA12 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG222690 representing NM_181678
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATTATCCAGATGAAAACACCTATGGAAATAAAGCTGACATTATCTCTACGGTAGAATTCAACCACA CGGGAGAATTACTAGCGACAGGGGACAAGGGGGGTCGGGTTGTAATATTTCAACGAGAGCAGGAGAGTAA AAATCAGGTTCATCGTAGGGGTGAATACAATGTTTACAGCACATTCCAGAGCCATGAACCCGAGTTCGAT TACCTGAAGAGTTTAGAAATAGAAGAAAAAATCAATAAAATAAGATGGCTCCCCCAGCAGAATGCAGCTT ACTTTCTTCTGTCTACTAATGATAAAACTGTGAAGCTGTGGAAAGTCAGCGAGCGTGATAAGAGGCCAGA AGGCTACAATCTGAAAGATGAGGAGGGCCGGCTCCGGGATCCTGCCACCATCACAACCCTGCGGGTGCCT GTCCTGAGACCCATGGACCTGATGGTGGAGGCCACCCCACGAAGAGTATTTGCCAACGCACACACATATC ACATCAACTCCATATCTGTCAACAGCGACTATGAAACCTACATGTCCGCTGATGACCTGAGGATTAACCT ATGGAACTTTGAAATAACCAATCAAAGTTTTAATATTGTGGACATTAAGCCAGCCAACATGGAGGAGCTC ACGGAGGTGATCACAGCAGCCGAGTTCCACCCCCATCATTGCAACACCTTCGTGTACAGCAGCAGCAAAG GGACAATCCGGCTGTGTGACATGCGGGCATCTGCCCTGTGTGACAGGCACACCAAATTTTTTGAAGAGCC GGAAGATCCAAGCAACAGATCATTTTTCTCTGAAATTATCTCTTCGATTTCGGATGTGAAGTTCAGCCAC AGTGGGAGGTATATCATGACCAGGGACTACTTGACCGTCAAAGTCTGGGATCTCAACATGGAAAACCGCC CCATCGAGACTTACCAGGTTCATGACTACCTCCGCAGCAAGCTGTGTTCCCTCTATGAAAATGACTGCAT TTTTGATAAATTTGAGTGTGTGTGGAATGGGTCAGACAGTGTCATCATGACAGGCTCCTACAACAACTTC TTCAGGATGTTCGACAGAAACACCAAGCGTGATGTGACCCTTGAGGCTTCGAGGGAAAACAGCAAGCCCC GGGCTATCCTCAAACCCCGAAAAGTGTGTGTGGGGGGCAAGCGGAGAAAAGACGAGATCAGTGTCGACAG TCTGGACTTTAGCAAAAAGATCTTGCATACAGCTTGGCATCCTTCAGAAAATATTATAGCAGTGGCGGCT ACAAATAACCTATATATATTCCAGGACAAGGTTAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222690 representing NM_181678
Red=Cloning site Green=Tags(s) MNYPDENTYGNKADIISTVEFNHTGELLATGDKGGRVVIFQREQESKNQVHRRGEYNVYSTFQSHEPEFD YLKSLEIEEKINKIRWLPQQNAAYFLLSTNDKTVKLWKVSERDKRPEGYNLKDEEGRLRDPATITTLRVP VLRPMDLMVEATPRRVFANAHTYHINSISVNSDYETYMSADDLRINLWNFEITNQSFNIVDIKPANMEEL TEVITAAEFHPHHCNTFVYSSSKGTIRLCDMRASALCDRHTKFFEEPEDPSNRSFFSEIISSISDVKFSH SGRYIMTRDYLTVKVWDLNMENRPIETYQVHDYLRSKLCSLYENDCIFDKFECVWNGSDSVIMTGSYNNF FRMFDRNTKRDVTLEASRENSKPRAILKPRKVCVGGKRRKDEISVDSLDFSKKILHTAWHPSENIIAVAA TNNLYIFQDKVN TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_181678 |
ORF Size | 1296 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_181678.1, NP_858064.1 |
RefSeq Size | 2020 bp |
RefSeq ORF | 1299 bp |
Locus ID | 5521 |
Cytogenetics | 5q32 |
Protein Families | Druggable Genome, Phosphatase |
Protein Pathways | Tight junction |
Gene Summary | 'The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a beta isoform of the regulatory subunit B55 subfamily. Defects in this gene cause autosomal dominant spinocerebellar ataxia 12 (SCA12), a disease caused by degeneration of the cerebellum, sometimes involving the brainstem and spinal cord, and in resulting in poor coordination of speech and body movements. Multiple alternatively spliced variants, which encode different isoforms, have been identified for this gene. The 5' UTR of some of these variants includes a CAG trinucleotide repeat sequence (7-28 copies) that can be expanded to 55-78 copies in cases of SCA12. [provided by RefSeq, Jul 2016]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC307343 | PPP2R2B (untagged)-Human protein phosphatase 2, regulatory subunit B, beta (PPP2R2B), transcript variant 6 |
USD 730.00 |
|
RC222690 | PPP2R2B (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B, beta (PPP2R2B), transcript variant 6 |
USD 420.00 |
|
RC222690L3 | Lenti-ORF clone of PPP2R2B (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B, beta (PPP2R2B), transcript variant 6 |
USD 620.00 |
|
RC222690L4 | Lenti-ORF clone of PPP2R2B (mGFP-tagged)-Human protein phosphatase 2, regulatory subunit B, beta (PPP2R2B), transcript variant 6 |
USD 620.00 |
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