AIPL1 (NM_001033055) Human Tagged ORF Clone

CAT#: RG223173

AIPL1 (GFP-tagged) - Human aryl hydrocarbon receptor interacting protein-like 1 (AIPL1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AIPL1
• Synonyms: AIPL2; LCA4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223173 representing NM_001033055
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCCGCTCTGCTCCTGAACGTGGAAGGGGTCAAGAAAACCATTCTGCACGGGGGCACGGGCGAGC
  TCCCAAACTTCATCACCGGATCCCGACACACGGGGGTCTACCCCATCCTATCCCGGAGCCTGAGGCAGAT
  GGCCCAGGGCAAGGACCCCACAGAGTGGCACGTGCACACGTGCGGGCTGGCCAACATGTTCGCCTACCAC
  ACGCTGGGCTACGAGGACCTGGACGAGCTGCAGAAGGAGCCTCAGCCTCTGGTCTTTGTGATCGAGCTGC
  TGCAGGTTGATGCCCCGAGTGATTACCAGAGGGAGACCTGGAACCTGAGCAATCATGAGAAGATGAAGGC
  GGTGCCCGTCCTCCACGGAGAGGGAAATCGGCTCTTCAAGCTGGGCCGCTACGAGGAGGCCTCTTCCAAG
  TACCAGGAGGCCATCATCTGCCTAAGGAACCTGCAGACCAAGGAGAAGCCATGGGAGGTGCAGTGGCTGA
  AGCTGGAGAAGATGATCAATACTCTGATCCTCAACTACTGCCAGTGCCTGCTGAAGAAGGAGGAGTACTA
  TGAGGTGCTGGAGCACACCAGTGATATTCTCCGGCACCACCCAGGCATCGTGAAGGCCTACTACGTGCGT
  GCCCGGGCTCACGCAGAGGTGTGGAATGAGGCCGAGGCCAAGGCGGACCTCCAGAAAGTGCTGGAGCTGG
  AGCCGTCCATGCAGAAGGCGGTGCGCAGGGAGCTGAGGCTGCTGGAGAACCGCATGGCGGAGAAGCAGGA
  GGAGGAGCGGCTGCGCTGCCGGAACATGCTGAGCCAGGGTGCCACGCAGCCTCCCGCAGAGCCACCCACA
  GAGCCACCCGCACAGTCATCCACAGAGCCACCTGCAGAGCCACCCACAGCACCATCTGCAGAGCTGTCCG
  CAGGGCCCCCTGCAGAGCCAGCCACAGAGCCACCCCCGTCCCCAGGGCACTCGCTGCAGCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223173 representing NM_001033055
  Red=Cloning site Green=Tags(s)
  
  MDAALLLNVEGVKKTILHGGTGELPNFITGSRHTGVYPILSRSLRQMAQGKDPTEWHVHTCGLANMFAYH
  TLGYEDLDELQKEPQPLVFVIELLQVDAPSDYQRETWNLSNHEKMKAVPVLHGEGNRLFKLGRYEEASSK
  YQEAIICLRNLQTKEKPWEVQWLKLEKMINTLILNYCQCLLKKEEYYEVLEHTSDILRHHPGIVKAYYVR
  ARAHAEVWNEAEAKADLQKVLELEPSMQKAVRRELRLLENRMAEKQEEERLRCRNMLSQGATQPPAEPPT
  EPPAQSSTEPPAEPPTAPSAELSAGPPAEPATEPPPSPGHSLQH
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033055
• ORF Size: 972 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001033055.1, NP_001028227.1
• RefSeq Size: 2801
• RefSeq ORF: 975
• Locus ID: 23746
• Protein Families: Druggable Genome
• Gene Summary: Leber congenital amaurosis (LCA) is the most severe inherited retinopathy with the earliest age of onset and accounts for at least 5% of all inherited retinal diseases. Affected individuals are diagnosed at birth or in the first few months of life with nystagmus, severely impaired vision or blindness and an abnormal or flat electroretinogram. The photoreceptor/pineal-expressed gene, AIPL1, encoding aryl-hydrocarbon interacting protein-like 1, is located within the LCA4 candidate region. The encoded protein contains three tetratricopeptide motifs, consistent with chaperone or nuclear transport activity. Mutations in this gene may cause approximately 20% of recessive LCA. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]