KCNK10 (NM_138317) Human Tagged ORF Clone

CAT#: RG223229

KCNK10 (GFP-tagged) - Human potassium channel, subfamily K, member 10 (KCNK10), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KCNK10
• Synonyms: K2p10.1; PPP1R97; TREK-2; TREK2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223229 representing NM_138317
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAATTTCCAATCGAGACGCCAAGAAAACAGGTGAACTGGGATCCTAAAGTGGCCGTTCCCGCAGCAG
  CACCGGTGTGCCAGCCCAAGAGCGCCACTAACGGGCAACCCCCGGCTCCGGCTCCGACTCCAACTCCGCG
  CCTGTCCATTTCCTCCCGAGCCACAGTGGTAGCCAGGATGGAAGGCACCTCCCAAGGGGGCTTGCAGACC
  GTCATGAAGTGGAAGACGGTGGTTGCCATCTTTGTGGTTGTGGTGGTCTACCTTGTCACTGGCGGTCTTG
  TCTTCCGGGCATTGGAGCAGCCCTTTGAGAGCAGCCAGAAGAATACCATCGCCTTGGAGAAGGCGGAATT
  CCTGCGGGATCATGTCTGTGTGAGCCCCCAGGAGCTGGAGACGTTGATCCAGCATGCTCTTGATGCTGAC
  AATGCGGGAGTCAGTCCAATAGGAAACTCTTCCAACAACAGCAGCCACTGGGACCTCGGCAGTGCCTTTT
  TCTTTGCTGGAACTGTCATTACGACCATAGGGTATGGGAATATTGCTCCGAGCACTGAAGGAGGCAAAAT
  CTTTTGTATTTTATATGCCATCTTTGGAATTCCACTCTTTGGTTTCTTATTGGCTGGAATTGGAGACCAA
  CTTGGAACCATCTTTGGGAAAAGCATTGCAAGAGTGGAGAAGGTCTTTCGAAAAAAGCAAGTGAGTCAGA
  CCAAGATCCGGGTCATCTCAACCATCCTGTTCATCTTGGCCGGCTGCATTGTGTTTGTGACGATCCCTGC
  TGTCATCTTTAAGTACATCGAGGGCTGGACGGCCTTGGAGTCCATTTACTTTGTGGTGGTCACTCTGACC
  ACGGTGGGCTTTGGTGATTTTGTGGCAGGGGGAAACGCTGGCATCAATTATCGGGAGTGGTATAAGCCCC
  TAGTGTGGTTTTGGATCCTTGTTGGCCTTGCCTACTTTGCAGCTGTCCTCAGTATGATCGGAGATTGGCT
  ACGGGTTCTGTCCAAAAAGACAAAAGAAGAGGTGGGTGAAATCAAGGCCCATGCGGCAGAGTGGAAGGCC
  AATGTCACGGCTGAGTTCCGGGAGACACGGCGAAGGCTCAGCGTGGAGATCCACGATAAGCTGCAGCGGG
  CGGCCACCATCCGCAGCATGGAGCGCCGGCGGCTGGGCCTGGACCAGCGGGCCCACTCACTGGACATGCT
  GTCCCCCGAGAAGCGCTCTGTCTTTGCTGCCCTGGACACCGGCCGCTTCAAGGCCTCATCCCAGGAGAGC
  ATCAACAACCGGCCCAACAACCTGCGCCTGAAGGGGCCGGAGCAGCTGAACAAGCATGGGCAGGGTGCGT
  CCGAGGACAACATCATCAACAAGTTCGGGTCCACCTCCAGACTCACCAAGAGGAAAAACAAGGACCTCAA
  AAAGACCTTGCCCGAGGACGTTCAGAAAATCTACAAGACCTTCCGGAATTACTCCCTGGACGAGGAGAAG
  AAAGAGGAGGAGACGGAAAAGATGTGTAACTCAGACAACTCCAGCACAGCCATGCTGACGGACTGTATCC
  AGCAGCACGCTGAGTTGGAGAACGGAATGATACCCACGGACACCAAAGACCGGGAGCCGGAGAACAACTC
  ATTACTTGAAGACAGAAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223229 representing NM_138317
  Red=Cloning site Green=Tags(s)
  
  MKFPIETPRKQVNWDPKVAVPAAAPVCQPKSATNGQPPAPAPTPTPRLSISSRATVVARMEGTSQGGLQT
  VMKWKTVVAIFVVVVVYLVTGGLVFRALEQPFESSQKNTIALEKAEFLRDHVCVSPQELETLIQHALDAD
  NAGVSPIGNSSNNSSHWDLGSAFFFAGTVITTIGYGNIAPSTEGGKIFCILYAIFGIPLFGFLLAGIGDQ
  LGTIFGKSIARVEKVFRKKQVSQTKIRVISTILFILAGCIVFVTIPAVIFKYIEGWTALESIYFVVVTLT
  TVGFGDFVAGGNAGINYREWYKPLVWFWILVGLAYFAAVLSMIGDWLRVLSKKTKEEVGEIKAHAAEWKA
  NVTAEFRETRRRLSVEIHDKLQRAATIRSMERRRLGLDQRAHSLDMLSPEKRSVFAALDTGRFKASSQES
  INNRPNNLRLKGPEQLNKHGQGASEDNIINKFGSTSRLTKRKNKDLKKTLPEDVQKIYKTFRNYSLDEEK
  KEEETEKMCNSDNSSTAMLTDCIQQHAELENGMIPTDTKDREPENNSLLEDRN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138317
• ORF Size: 1629 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_138317.1, NP_612190.1
• RefSeq Size: 2484
• RefSeq ORF: 1632
• Locus ID: 54207
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: The protein encoded by this gene belongs to the family of potassium channel proteins containing two pore-forming P domains. This channel is an open rectifier which primarily passes outward current under physiological K+ concentrations, and is stimulated strongly by arachidonic acid and to a lesser degree by membrane stretching, intracellular acidification, and general anaesthetics. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Sep 2008]