PPEF1 (NM_152226) Human Tagged ORF Clone
CAT#: RG223242
- TrueORF®
PPEF1 (GFP-tagged) - Human protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant 3
"NM_152226" in other vectors (4)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | PPEF1 |
Synonyms | PP7; PPEF; PPP7C; PPP7CA |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG223242 representing NM_152226
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGATGCAGCAGTTCTTCAACGAAAACCAGGAGATCTGACACATCACTGAGAGCTGCGTTGATCATCC AGAACTGGTACCGAGGTTACAAAGCTCGACTGAAGGCCAGACAACACTATGCCCTCACCATCTTCCAGTC CATCGAATATGCTGATGAACAAGGCCAAATGCAGTTATCCACCTTCTTTTCCTTCATGTTGGAAAACTAC ACACATATACATAAGGAAGAGCTAGAATTAAGAAATCAGTCTCTTGAAAGCGAACAGGACATGAGGGATA GATGGGATTATGTGGACTCGATAGATGTCCCAGACTCCTATAATGGTCCTCGGCTACAATTTCCTCTCAC TTGTACGGATATTGATTTACTTCTTGAGGCCTTCAAGGAACAACAGATACTTCATGCCCATTATGTCTTA GAGGTGCTATTTGAAACCAAGAAAGTCCTGAAGCAAATGCCGAATTTCACTCACATACAAACTTCTCCCT CCAAAGAGGTAACAATCTGTGGTGATTTGCATGGGAAACTGGATGATCTTTTTTTGATCTTCTACAAGAA TGGTCTCCCCTCAGAGAGGAACCCGTATGTTTTTAATGGTGACTTTGTAGATCGAGGAAAGAATTCCATA GAGATCCTAATGATCCTGTGTGTGAGTTTTCTTGTCTACCCCAATGACCTGCACTTGAACAGAGGGAACC ACGAAGATTTTATGATGAATCTGAGGTATGGCTTCACGAAAGAAATTTTGCATAAATATAAGCTACATGG AAAAAGAATCTTACAAATCTTGGAAGAATTCTATGCCTGGCTCCCAATCGGTACAATCGTTGACAATGAA ATCCTGGTCATCCATGGTGGGATATCAGAGACCACAGACTTGAATTTACTCCACCGTGTAGAGAGGAACA AGATGAAATCTGTGCTGATACCACCAACGGAAACAAACAGAGACCATGACACTGACTCGAAGCACAATAA AGTAGGTGTGACTTTTAATGCACATGGAAGAATCAAAACAAATGGATCTCCTACTGAACACTTAACAGAG CATGAATGGGAACAGGTGGTGACTATATTTTCTGCTTCTAATTATTATGAAGAAGGCAGCAATCGAGGAG CTTACATCAAACTATGTTCTGGTACAACTCCTCGATTTTTCCAGTACCAAGTAACTAAAGCAACGTGCTT TCAGCCTCTTCGCCAAAGAGTGGATACTATGGAAAACAGCGCCATCAAGATATTAAGAGAGAGAGTGATT TCACGAAAAAGTGACCTTACTCGTGCTTTCCAACTTCAAGACCACAGAAAATCAGGAAAACTTTCTGTGA GCCAGTGGGCTTTTTGCATGGAGAACATTTTGGGGCTGAACTTACCATGGAGATCCCTCAGTTCGAATCT GGTAAACATAGACCAAAATGGAAACGTTGAATACATGTCCAGCTTCCAGAATATCCGCATTGAAAAACCT GTACAAGAGGCTCATTCTACTCTAGTTGAAACTCTGTACAGATACAGATCTGACCTGGAAATCATATTTA ATGCCATTGACACTGATCACTCAGGCCTGATCTCCGTGGAAGAATTTCGTGCCATGTGGAAACTTTTTAG TTCTCACTACAATGTTCACATTGATGATTCCCAAGTCAATAAGCTTGCCAACATAATGGACTTGAACAAA GATGGAAGCATTGACTTTAATGAGTTTTTAAAGGCTTTCTATGTAGTGCATAGATATGAAGACTTGATGA AACCTGATGTCACCAACCTTGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223242 representing NM_152226
Red=Cloning site Green=Tags(s) MGCSSSSTKTRRSDTSLRAALIIQNWYRGYKARLKARQHYALTIFQSIEYADEQGQMQLSTFFSFMLENY THIHKEELELRNQSLESEQDMRDRWDYVDSIDVPDSYNGPRLQFPLTCTDIDLLLEAFKEQQILHAHYVL EVLFETKKVLKQMPNFTHIQTSPSKEVTICGDLHGKLDDLFLIFYKNGLPSERNPYVFNGDFVDRGKNSI EILMILCVSFLVYPNDLHLNRGNHEDFMMNLRYGFTKEILHKYKLHGKRILQILEEFYAWLPIGTIVDNE ILVIHGGISETTDLNLLHRVERNKMKSVLIPPTETNRDHDTDSKHNKVGVTFNAHGRIKTNGSPTEHLTE HEWEQVVTIFSASNYYEEGSNRGAYIKLCSGTTPRFFQYQVTKATCFQPLRQRVDTMENSAIKILRERVI SRKSDLTRAFQLQDHRKSGKLSVSQWAFCMENILGLNLPWRSLSSNLVNIDQNGNVEYMSSFQNIRIEKP VQEAHSTLVETLYRYRSDLEIIFNAIDTDHSGLISVEEFRAMWKLFSSHYNVHIDDSQVNKLANIMDLNK DGSIDFNEFLKAFYVVHRYEDLMKPDVTNLG TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_152226 |
ORF Size | 1773 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_152226.1, NP_689412.1 |
RefSeq Size | 2687 bp |
RefSeq ORF | 1776 bp |
Locus ID | 5475 |
Cytogenetics | Xp22.13 |
Domains | IQ, EFh, Metallophos |
Protein Families | Druggable Genome, Phosphatase |
Gene Summary | 'This gene encodes a member of the serine/threonine protein phosphatase with EF-hand motif family. The protein contains a protein phosphatase catalytic domain, and at least two EF-hand calcium-binding motifs in its C terminus. Although its substrate(s) is unknown, the encoded protein has been suggested to play a role in specific sensory neuron function and/or development. This gene shares high sequence similarity with the Drosophila retinal degeneration C (rdgC) gene. Several alternatively spliced transcript variants, each encoding a distinct isoform, have been described. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC314127 | PPEF1 (untagged)-Human protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant 3 |
USD 1,010.00 |
|
RC223242 | PPEF1 (Myc-DDK-tagged)-Human protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant 3 |
USD 480.00 |
|
RC223242L3 | Lenti-ORF clone of PPEF1 (Myc-DDK-tagged)-Human protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant 3 |
USD 680.00 |
|
RC223242L4 | Lenti-ORF clone of PPEF1 (mGFP-tagged)-Human protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant 3 |
USD 680.00 |
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