PDLIM5 (NM_001011513) Human Tagged ORF Clone

CAT#: RG223267

PDLIM5 (GFP-tagged) - Human PDZ and LIM domain 5 (PDLIM5), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PDLIM5
• Synonyms: ENH; ENH1; L9; LIM
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223267 representing NM_001011513
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAACTACAGTGTGTCACTGGTTGGCCCAGCTCCTTGGGGTTTCCGGCTGCAGGGCGGTAAGGATT
  TCAACATGCCTCTGACAATCTCTAGTCTAAAAGATGGCGGCAAGGCAGCCCAGGCAAATGTAAGAATAGG
  CGATGTGGTTCTCAGCATTGATGGAATAAATGCACAAGGAATGACTCATCTTGAAGCCCAGAATAAGATT
  AAGGGTTGTACAGGCTCTTTGAATATGACTCTGCAAAGAGCATCTGCTGCACCCAAGCCTGAGCCGGTTC
  CTGTTCAAAAGCCCACAGTCACCAGCGTGTGTTCCGAGACTTCTCAGGAGCTAGCAGAGGGACAGAGAAG
  AGGATCCCAGGGTGACAGTAAACAGCAAAATGGCCCACCAAGAAAACACATTGTGGAGCGCTATACAGAG
  TTTTATCATGTACCCACTCACAGTGATGCCAGCAAGAAGAGACTGATTGAGGATACTGAAGACTGGCGTC
  CAAGGACTGGAACAACTCAGTCTCGCTCTTTCCGAATCCTTGCCCAGATCACTGGGACTGAACATTTGAA
  AGAATCTGAAGCCGATAATACAAAGAAGGCAAATAACTCTCAGGAGCCTTCTCCGCAGTTGGCTTCCTCG
  GTAGCTTCCACACGGAGCATGCCCGAGAGCCTGGACAGCCCAACCTCTGGCAGACCAGGGGTTACCAGCC
  TCACAACTGCAGCTGCCTTCAAGCCTGTAGGATCCACTGGCGTCATCAAGTCACCAAGCTGGCAACGGCC
  AAACCAAGGAGTACCTTCCACTGGAAGAATCTCAAACAGCGCTACTTACTCAGGATCAGTGGCACCAGCC
  AACTCAGCTTTGGGACAAACCCAGCCAAGTGACCAGGACACTTTAGTGCAAAGAGCTGAGCACATTCCAG
  CAGGGAAACGAACTCCGATGTGCGCCCATTGTAACCAGGTCATCAGAGGACCATTCTTAGTGGCACTGGG
  GAAATCTTGGCACCCAGAAGAATTCAACTGCGCTCACTGCAAAAATACAATGGCCTACATTGGATTTGTA
  GAGGAGAAAGGAGCCCTGTATTGTGAGCTGTGCTATGAGAAATTCTTTGCCCCTGAATGTGGTCGATGCC
  AAAGGAAGATCCTTGGAGAAGTCATCAATGCGTTGAAACAAACTTGGCATGTTTCCTGTTTTGTGTGTGT
  AGCCTGTGGAAAGCCCATTCGGAACAATGTTTTTCACTTGGAGGATGGTGAACCCTACTGTGAGACTGAT
  TATTATGCCCTCTTTGGTACTATATGCCATGGATGTGAATTTCCCATAGAAGCTGGTGACATGTTCCTGG
  AAGCTCTGGGCTACACCTGGCATGACACTTGCTTTGTATGCTCAGTGTGTTGTGAAAGTTTGGAAGGTCA
  GACCTTTTTCTCCAAGAAGGACAAGCCCCTGTGTAAGAAACATGCTCATTCTGTGAATTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223267 representing NM_001011513
  Red=Cloning site Green=Tags(s)
  
  MSNYSVSLVGPAPWGFRLQGGKDFNMPLTISSLKDGGKAAQANVRIGDVVLSIDGINAQGMTHLEAQNKI
  KGCTGSLNMTLQRASAAPKPEPVPVQKPTVTSVCSETSQELAEGQRRGSQGDSKQQNGPPRKHIVERYTE
  FYHVPTHSDASKKRLIEDTEDWRPRTGTTQSRSFRILAQITGTEHLKESEADNTKKANNSQEPSPQLASS
  VASTRSMPESLDSPTSGRPGVTSLTTAAAFKPVGSTGVIKSPSWQRPNQGVPSTGRISNSATYSGSVAPA
  NSALGQTQPSDQDTLVQRAEHIPAGKRTPMCAHCNQVIRGPFLVALGKSWHPEEFNCAHCKNTMAYIGFV
  EEKGALYCELCYEKFFAPECGRCQRKILGEVINALKQTWHVSCFVCVACGKPIRNNVFHLEDGEPYCETD
  YYALFGTICHGCEFPIEAGDMFLEALGYTWHDTCFVCSVCCESLEGQTFFSKKDKPLCKKHAHSVNF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001011513
• ORF Size: 1461 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001011513.1, NP_001011513.1
• RefSeq Size: 5774
• RefSeq ORF: 1464
• Locus ID: 10611
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of a family of proteins that possess a 100-amino acid PDZ domain at the N terminus and one to three LIM domains at the C-terminus. This family member functions as a scaffold protein that tethers protein kinases to the Z-disk in striated muscles. It is thought to function in cardiomyocyte expansion and in restraining postsynaptic growth of excitatory synapses. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]