UGT2B10 (NM_001075) Human Tagged ORF Clone

CAT#: RG223408

UGT2B10 (GFP-tagged) - Human UDP glucuronosyltransferase 2 family, polypeptide B10 (UGT2B10), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: UGT2B10
• Synonyms: UDPGT2B10
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223408 representing NM_001075
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGAAATGGACTACAGTTCTGCTGATACAACTCAGTTTTTACTTTAGCTCTGGGAGTTGTGGAA
  AGGTGCTGGTATGGGCCGCAGAATACAGCCTTTGGATGAATATGAAGACAATCCTGAAAGAACTTGTTCA
  GAGAGGTCATGAGGTGACTGTACTGGCATCTTCAGCTTCCATTCTTTTTGATCCCAACGACTCATCCACT
  CTTAAACTTGAAGTTTATCCTACATCTTTAACTAAAACTGAATTTGAGAATATCATCATGCAATTGGTTA
  AGAGATTGTCAGAAATTCAAAAAGATACATTTTGGTTACCTTTTTCACAAGAACAAGAAATCCTGTGGGC
  AATTAATGACATAATTAGAAACTTCTGTAAAGATGTAGTTTCAAATAAGAAACTTATGAAAAAACTACAA
  GAGTCAAGATTTGACATCGTTTTTGCAGATGCTTATTTACCCTGTGGTGAGCTGCTGGCTGAGCTATTTA
  ACATACCCTTTGTGTACAGTCACAGCTTCAGTCCTGGCTACTCATTTGAAAGGCACAGTGGAGGATTTAT
  TTTCCCTCCTTCCTACGTACCTGTTGTTATGTCAAAATTAAGTGATCAAATGACTTTCATGGAGAGGGTA
  AAAAATATGCTCTATGTGCTTTATTTTGACTTTTGGTTCCAAATATTTAATATGAAGAAGTGGGATCAGT
  TTTACAGTGAAGTTTTAGGAAGACCCACTACATTATCTGAGACAATGAGGAAAGCTGACATATGGCTTAT
  GCGAAACTCCTGGAATTTTAAATTTCCTCATCCATTCTTACCAAATGTTGATTTTGTTGGAGGACTCCAC
  TGCAAACCTGCCAAACCCCTACCTAAGGAAATGGAGGAGTTTGTACAGAGCTCTGGAGAAAATGGTGTTG
  TGGTGTTTTCTCTGGGGTCAATGGTCAGTAACATGACAGAAGAAAGGGCCAACGTAATTGCAACAGCCCT
  TGCCAAGATCCCACAAAAGGTTCTTTGGAGATTTGATGGGAATAAACCAGATGCCTTAGGTCTCAATACT
  CGACTGTACAAGTGGATACCCCAGAATGACCTTCTAGGTCATCCAAAAACCAGAGCTTTTATAACTCATG
  GTGGAGCCAATGGCATCTATGAGGCAATCTACCATGGGATCCCTATGGTGGGCATTCCATTGTTTTTTGA
  TCAACCTGATAATATTGCTCACATGAAGGCCAAGGGAGCAGCTGTTAGAGTGGACTTCAACACAATGTCG
  AGTACAGACCTGCTGAATGCACTGAAGACAGTAATTAATGATCCTTCATATAAAGAGAATATTATGAAAT
  TATCAAGAATTCAACATGATCAACCAGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGAATTTGTCAT
  GCGCCACAAAGGAGCCAAACATCTTCGAGTTGCAGCCCACAACCTCACCTGGTTCCAGTACCACTCTTTG
  GATGTGATTGGGTTCCTGCTGGCTTGTGTGGCAACCGTGCTATTTATCATCACAAAGTGTTGTCTGTTTT
  GTTTCTGGAAGTTTGCTAGAAAAGGAAAGAAGGGAAAAAGGGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223408 representing NM_001075
  Red=Cloning site Green=Tags(s)
  
  MALKWTTVLLIQLSFYFSSGSCGKVLVWAAEYSLWMNMKTILKELVQRGHEVTVLASSASILFDPNDSST
  LKLEVYPTSLTKTEFENIIMQLVKRLSEIQKDTFWLPFSQEQEILWAINDIIRNFCKDVVSNKKLMKKLQ
  ESRFDIVFADAYLPCGELLAELFNIPFVYSHSFSPGYSFERHSGGFIFPPSYVPVVMSKLSDQMTFMERV
  KNMLYVLYFDFWFQIFNMKKWDQFYSEVLGRPTTLSETMRKADIWLMRNSWNFKFPHPFLPNVDFVGGLH
  CKPAKPLPKEMEEFVQSSGENGVVVFSLGSMVSNMTEERANVIATALAKIPQKVLWRFDGNKPDALGLNT
  RLYKWIPQNDLLGHPKTRAFITHGGANGIYEAIYHGIPMVGIPLFFDQPDNIAHMKAKGAAVRVDFNTMS
  STDLLNALKTVINDPSYKENIMKLSRIQHDQPVKPLDRAVFWIEFVMRHKGAKHLRVAAHNLTWFQYHSL
  DVIGFLLACVATVLFIITKCCLFCFWKFARKGKKGKRD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001075
• ORF Size: 1584 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001075.2, NP_001066.1
• RefSeq Size: 1620 bp
• RefSeq ORF: 1587 bp
• Locus ID: 7365
• Cytogenetics: 4q13.2
• Protein Families: Transmembrane
• Protein Pathways: Androgen and estrogen metabolism, Ascorbate and aldarate metabolism, Drug metabolism - cytochrome P450, Drug metabolism - other enzymes, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Pentose and glucuronate interconversions, Porphyrin and chlorophyll metabolism, Retinol metabolism, Starch and sucrose metabolism
• Gene Summary: ''