GABA A Receptor alpha 6 (GABRA6) (NM_000811) Human Tagged ORF Clone

CAT#: RG223410

GABRA6 (GFP-tagged) - Human gamma-aminobutyric acid (GABA) A receptor, alpha 6 (GABRA6)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GABRA6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223410 representing NM_000811
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCGTCTCTGCCCTGGCTGTGCATTATTCTGTGGCTAGAAAATGCCCTAGGGAAACTCGAAGTTG
  AAGGCAACTTCTACTCAGAAAACGTCAGTCGGATCCTGGACAACTTGCTTGAAGGCTATGACAATCGGCT
  GCGGCCGGGATTTGGAGGTGCTGTCACTGAAGTCAAAACAGACATTTATGTGACCAGTTTTGGGCCCGTG
  TCAGATGTGGAGATGGAGTATACGATGGATGTTTTTTTCCGCCAGACCTGGACTGATGAGAGGTTGAAGT
  TTGGGGGGCCAACTGAGATTCTGAGTCTGAATAATTTGATGGTCAGTAAAATCTGGACGCCTGACACCTT
  TTTCAGAAATGGTAAAAAGTCCATTGCTCACAACATGACAACTCCTAATAAACTCTTCAGAATAATGCAG
  AATGGAACCATTTTATACACCATGAGGCTTACCATCAATGCTGACTGTCCCATGAGGCTGGTTAACTTTC
  CTATGGATGGGCATGCTTGTCCACTCAAGTTTGGGAGCTATGCTTATCCCAAAAGTGAAATCATATATAC
  GTGGAAAAAAGGACCACTTTACTCAGTAGAAGTCCCAGAAGAATCTTCAAGCCTTCTCCAGTATGATCTG
  ATTGGACAAACAGTATCTAGTGAGACAATTAAATCTAACACAGGTGAATACGTTATAATGACAGTTTACT
  TCCACTTGCAAAGGAAGATGGGCTACTTCATGATACAGATATACACTCCTTGCATTATGACAGTCATTCT
  TTCCCAGGTGTCTTTCTGGATTAATAAGGAGTCCGTCCCAGCAAGAACTGTTTTTGGGATCACCACTGTT
  TTAACTATGACCACTTTGAGCATCAGTGCCCGGCACTCTTTGCCAAAAGTGTCATATGCCACTGCCATGG
  ATTGGTTCATAGCTGTTTGCTTTGCATTCGTCTTCTCTGCTCTTATCGAGTTCGCAGCTGTCAACTACTT
  TACCAATCTTCAGACACAGAAGGCCAAAAGGAAGGCACAGTTTGCAGCCCCACCCACAGTGACAATATCA
  AAAGCTACTGAACCTTTGGAAGCTGAGATTGTTTTGCATCCTGACTCCAAATATCATCTGAAGAAAAGGA
  TCACTTCTCTGTCTTTGCCAATAGTTTCATCTTCCGAGGCCAATAAAGTGCTCACGAGAGCGCCCATCTT
  ACAATCAACACCTGTCACACCCCCACCACTCTCGCCAGCCTTTGGAGGCACCAGTAAAATAGACCAGTAT
  TCTCGAATTCTCTTCCCAGTTGCATTTGCAGGATTCAACCTTGTGTACTGGGTAGTTTATCTTTCCAAAG
  ATACAATGGAAGTGAGTAGCAGTGTTGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223410 representing NM_000811
  Red=Cloning site Green=Tags(s)
  
  MASSLPWLCIILWLENALGKLEVEGNFYSENVSRILDNLLEGYDNRLRPGFGGAVTEVKTDIYVTSFGPV
  SDVEMEYTMDVFFRQTWTDERLKFGGPTEILSLNNLMVSKIWTPDTFFRNGKKSIAHNMTTPNKLFRIMQ
  NGTILYTMRLTINADCPMRLVNFPMDGHACPLKFGSYAYPKSEIIYTWKKGPLYSVEVPEESSSLLQYDL
  IGQTVSSETIKSNTGEYVIMTVYFHLQRKMGYFMIQIYTPCIMTVILSQVSFWINKESVPARTVFGITTV
  LTMTTLSISARHSLPKVSYATAMDWFIAVCFAFVFSALIEFAAVNYFTNLQTQKAKRKAQFAAPPTVTIS
  KATEPLEAEIVLHPDSKYHLKKRITSLSLPIVSSSEANKVLTRAPILQSTPVTPPPLSPAFGGTSKIDQY
  SRILFPVAFAGFNLVYWVVYLSKDTMEVSSSVE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000811
• ORF Size: 1359 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000811.1, NP_000802.1
• RefSeq Size: 1732 bp
• RefSeq ORF: 1362 bp
• Locus ID: 2559
• Cytogenetics: 5q34
• Domains: Neur_chan_memb, Neur_chan_LBD
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: 'GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. [provided by RefSeq, Jul 2008]'