ATP6V1C2 (NM_001039362) Human Tagged ORF Clone

CAT#: RG223434

ATP6V1C2 (GFP-tagged) - Human ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C2 (ATP6V1C2), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ATP6V1C2
• Synonyms: ATP6C2; VMA5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223434 representing NM_001039362
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGAGTTTTGGTTAATTTCTGCCCCTGGCGATAAGGAAAATTTGCAAGCTCTGGAGAGGATGAATA
  CTGTAACCTCCAAGTCCAACCTGTCTTATAATACCAAATTCGCTATTCCTGACTTCAAGGTGGGGACCTT
  GGATTCCCTGGTTGGCCTCTCTGATGAGTTGGGGAAACTCGACACCTTTGCTGAAAGCCTCATAAGGAGA
  ATGGCTCAGAGCGTGGTGGAAGTCATGGAGGACTCAAAGGGGAAGGTCCAGGAGCACCTCCTGGCAAACG
  GAGTTGACTTAACATCCTTTGTGACCCACTTTGAATGGGACATGGCCAAATATCCTGTCAAGCAGCCGCT
  CGTGAGTGTGGTGGACACAATAGCCAAGCAACTGGCGCAGATCGAGATGGACCTGAAGTCCCGAACGGCC
  GCCTACAACACTCTGAAGACAAACCTGGAGAACCTGGAAAAGAAATCCATGGGGAACCTCTTCACCCGGA
  CACTGAGTGATATTGTGAGCAAAGAGGACTTCGTGCTGGATTCTGAATATCTCGTCACACTTCTGGTCAT
  CGTCCCCAAACCAAACTACTCACAATGGCAAAAAACCTACGAATCTCTCTCAGACATGGTGGTCCCTCGA
  TCAACCAAACTCATTACTGAGGACAAGGAAGGGGGCCTTTTCACTGTGACTCTGTTTCGAAAAGTGATTG
  AAGATTTCAAAACCAAGGCCAAAGAAAACAAGTTCACTGTTCGTGAATTTTACTATGATGAGAAGGAAAT
  TGAAAGGGAAAGGGAGGAGATGGCCAGATTGCTGTCTGATAAGAAGCAACAGTATCAAACTTCCTGTGTT
  GCTCTTAAAAAGGGATCATCCACCTTCCCGGACCACAAGGTTAAGGTAACCCCGCTAGGTAACCCTGATA
  GGCCTGCTGCGGGGCAGACCGACAGAGAGAGAGAGAGTGAGGGCGAGGGTGAGGGCCCCCTGCTGCGCTG
  GCTCAAGGTGAACTTCAGTGAAGCCTTCATTGCCTGGATCCACATCAAGGCCCTGAGAGTGTTTGTGGAG
  TCCGTGCTCAGGTATGGACTACCAGTGAACTTCCAGGCAGTGCTCCTGCAGCCGCATAAGAAGTCATCCA
  CCAAGCGTTTAAGAGAGGTTCTAAACTCTGTCTTCCGACATCTGGATGAAGTAGCCGCTACAAGTATACT
  GGATGCATCTGTGGAGATCCCGGGACTGCAACTCAATAACCAAGACTATTTTCCTTATGTCTACTTCCAT
  ATTGACCTTAGTCTTCTTGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223434 representing NM_001039362
  Red=Cloning site Green=Tags(s)
  
  MSEFWLISAPGDKENLQALERMNTVTSKSNLSYNTKFAIPDFKVGTLDSLVGLSDELGKLDTFAESLIRR
  MAQSVVEVMEDSKGKVQEHLLANGVDLTSFVTHFEWDMAKYPVKQPLVSVVDTIAKQLAQIEMDLKSRTA
  AYNTLKTNLENLEKKSMGNLFTRTLSDIVSKEDFVLDSEYLVTLLVIVPKPNYSQWQKTYESLSDMVVPR
  STKLITEDKEGGLFTVTLFRKVIEDFKTKAKENKFTVREFYYDEKEIEREREEMARLLSDKKQQYQTSCV
  ALKKGSSTFPDHKVKVTPLGNPDRPAAGQTDRERESEGEGEGPLLRWLKVNFSEAFIAWIHIKALRVFVE
  SVLRYGLPVNFQAVLLQPHKKSSTKRLREVLNSVFRHLDEVAATSILDASVEIPGLQLNNQDYFPYVYFH
  IDLSLLD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039362
• ORF Size: 1281 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001039362.1, NP_001034451.1
• RefSeq Size: 3250
• RefSeq ORF: 1284
• Locus ID: 245973
• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
• Gene Summary: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A,three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain C subunit isoforms. [provided by RefSeq, Jul 2008]