CHSY3 (NM_175856) Human Tagged ORF Clone

CAT#: RG223465

CHSY3 (GFP-tagged) - Human chondroitin sulfate synthase 3 (CHSY3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CHSY3
• Synonyms: CHSY2; CSS3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223465 representing NM_175856
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTGCGCTCTCGCCGCCCGTGGATGAGCGTGGCATTAGGGCTGGTGCTGGGCTTCACCGCCGCGT
  CCTGGCTCATCGCCCCCAGGGTGGCGGAGCTGAGCGAGAGGAAGAGACGTGGCTCCAGCCTCTGCTCCTA
  CTACGGTCGCTCTGCTGCTGGCCCCCGCGCCGGCGCTCAGCAGCCGCTCCCCCAGCCCCAGTCCCGACCA
  CGGCAGGAGCAGTCGCCGCCCCCCGCGCGCCAGGATCTCCAGGGGCCACCGCTGCCCGAGGCAGCACCCG
  GGATCACCAGTTTTCGAAGCAGCCCCTGGCAGCAGCCACCTCCGCTGCAGCAGCGGCGGCGAGGACGCGA
  GCCTGAGGGCGCGACGGGGCTTCCCGGTGCTCCAGCGGCCGAGGGGGAGCCCGAGGAGGAGGACGGGGGC
  GCGGCTGGGCAGCGGAGAGACGGCCGGCCGGGGAGTAGCCACAACGGCAGCGGGGACGGGGGCGCTGCCG
  CCCCGAGCGCCCGACCCCGGGACTTCCTGTACGTGGGGGTGATGACCGCGCAGAAGTACCTGGGCAGCCG
  CGCGCTGGCCGCGCAGCGGACCTGGGCGCGTTTCATCCCGGGCCGCGTGGAGTTCTTTTCCAGCCAGCAG
  CCCCCCAACGCCGGCCAGCCCCCGCCACCCCTGCCTGTCATCGCGCTACCGGGTGTGGACGACTCCTATC
  CTCCCCAGAAAAAGTCCTTCATGATGATCAAGTACATGCACGACCACTACCTGGACAAGTATGAGTGGTT
  CATGCGCGCCGACGACGATGTCTACATCAAAGGTGATAAATTAGAAGAGTTTCTTAGATCGCTAAACAGC
  AGTAAGCCTCTCTACCTGGGACAGACTGGCCTGGGGAATATTGAAGAGCTTGGAAAGCTGGGACTGGAGC
  CTGGGGAAAACTTCTGTATGGGAGGACCTGGCATGATCTTTAGCCGAGAAGTTCTCAGGAGGATGGTGCC
  ACATATTGGTGAATGCCTTAGAGAAATGTACACGACTCATGAGGATGTGGAAGTAGGAAGATGCGTTCGC
  CGTTTTGGTGGGACTCAGTGTGTCTGGTCTTACGAGATGCAACAACTGTTCCATGAAAATTATGAACACA
  ATCGGAAGGGTTACATCCAAGACCTTCACAATAGCAAAATCCATGCAGCCATAACACTTCATCCCAACAA
  AAGGCCTGCATACCAATACAGGCTGCATAATTACATGCTCAGCCGCAAAATTTCTGAACTTCGCTACCGC
  ACCATCCAGCTCCACAGGGAAAGTGCCCTGATGAGCAAGCTCAGTAACACAGAAGTGAGCAAAGAGGACC
  AGCAGCTGGGAGTGATACCTTCTTTCAACCACTTCCAGCCTCGGGAGAGAAATGAAGTGATAGAATGGGA
  GTTCCTGACAGGGAAGCTTCTATACTCAGCAGCTGAGAACCAGCCCCCTCGACAGAGCCTCAGTAGCATT
  TTAAGAACAGCACTGGATGATACCGTCCTACAGGTGATGGAGATGATCAATGAGAATGCCAAGAGCAGAG
  GACGGCTCATTGACTTCAAGGAAATTCAGTATGGCTACCGCAGAGTTAACCCCATGCACGGGGTGGAGTA
  CATTTTGGATTTACTCCTTTTATACAAAAGACACAAGGGAAGGAAACTGACTGTGCCAGTGAGACGTCAT
  GCCTATCTTCAGCAGTTGTTCAGCAAGCCTTTCTTCAGAGAGACCGAAGAGCTAGATGTCAACAGTCTTG
  TGGAGAGTATTAACAGTGAAACTCAGTCATTCTCCTTTATATCTAATTCTTTAAAGATATTATCTTCTTT
  TCAAGGTGCCAAAGAAATGGGAGGGCACAATGAAAAGAAAGTACACATTCTCGTTCCTCTCATCGGAAGG
  TATGACATTTTCTTGAGATTCATGGAGAACTTTGAAAACATGTGTCTTATCCCAAAGCAGAATGTAAAGT
  TGGTCATTATCCTTTTCAGTAGGGATTCTGGCCAAGACTCCAGCAAGCATATTGAGCTGATAAAAGGGTA
  CCAGAACAAGTACCCCAAAGCAGAAATGACCCTGATCCCAATGAAGGGAGAGTTTTCCAGAGGTCTTGGT
  CTTGAAATGGCTTCTGCCCAGTTTGACAATGACACTTTGCTGCTATTTTGTGATGTTGACTTGATCTTCA
  GAGAAGATTTTCTCCAACGATGTAGAGACAATACAATTCAGGGACAACAGGTGTACTATCCCATCATCTT
  TAGCCAGTATGACCCAAAGGTAACAAACGGGGGAAATCCTCCCACTGATGATTACTTCATATTCTCAAAA
  AAGACTGGATTTTGGAGAGACTATGGATATGGCATCACCTGTATTTACAAAAGTGATCTTCTAGGTGCAG
  GTGGATTTGATACCTCAATACAAGGCTGGGGACTAGAAGATGTAGATCTCTACAATAAAGTCATTCTATC
  TGGCTTAAGGCCATTCAGAAGCCAAGAAGTAGGAGTGGTGCATATTTTCCATCCAGTTCATTGTGATCCT
  AACTTGGACCCTAAGCAGTATAAGATGTGCTTAGGATCCAAGGCAAGTACTTTCGCCTCAACCATGCAAC
  TGGCTGAACTCTGGCTTGAAAAACATTTAGGTGTCAGGTACAATCGAACTCTCTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223465 representing NM_175856
  Red=Cloning site Green=Tags(s)
  
  MAVRSRRPWMSVALGLVLGFTAASWLIAPRVAELSERKRRGSSLCSYYGRSAAGPRAGAQQPLPQPQSRP
  RQEQSPPPARQDLQGPPLPEAAPGITSFRSSPWQQPPPLQQRRRGREPEGATGLPGAPAAEGEPEEEDGG
  AAGQRRDGRPGSSHNGSGDGGAAAPSARPRDFLYVGVMTAQKYLGSRALAAQRTWARFIPGRVEFFSSQQ
  PPNAGQPPPPLPVIALPGVDDSYPPQKKSFMMIKYMHDHYLDKYEWFMRADDDVYIKGDKLEEFLRSLNS
  SKPLYLGQTGLGNIEELGKLGLEPGENFCMGGPGMIFSREVLRRMVPHIGECLREMYTTHEDVEVGRCVR
  RFGGTQCVWSYEMQQLFHENYEHNRKGYIQDLHNSKIHAAITLHPNKRPAYQYRLHNYMLSRKISELRYR
  TIQLHRESALMSKLSNTEVSKEDQQLGVIPSFNHFQPRERNEVIEWEFLTGKLLYSAAENQPPRQSLSSI
  LRTALDDTVLQVMEMINENAKSRGRLIDFKEIQYGYRRVNPMHGVEYILDLLLLYKRHKGRKLTVPVRRH
  AYLQQLFSKPFFRETEELDVNSLVESINSETQSFSFISNSLKILSSFQGAKEMGGHNEKKVHILVPLIGR
  YDIFLRFMENFENMCLIPKQNVKLVIILFSRDSGQDSSKHIELIKGYQNKYPKAEMTLIPMKGEFSRGLG
  LEMASAQFDNDTLLLFCDVDLIFREDFLQRCRDNTIQGQQVYYPIIFSQYDPKVTNGGNPPTDDYFIFSK
  KTGFWRDYGYGITCIYKSDLLGAGGFDTSIQGWGLEDVDLYNKVILSGLRPFRSQEVGVVHIFHPVHCDP
  NLDPKQYKMCLGSKASTFASTMQLAELWLEKHLGVRYNRTLS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175856
• ORF Size: 2646 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_175856.4, NP_787052.3
• RefSeq Size: 3523
• RefSeq ORF: 2649
• Locus ID: 337876
• Protein Families: Transmembrane
• Protein Pathways: Chondroitin sulfate biosynthesis, Metabolic pathways
• Gene Summary: CSS3 is a glycosyltransferase that has both glucuronyltransferase and N-acetylgalactosaminyltransferase activities (Yada et al., 2003 [PubMed 12907687]). [supplied by OMIM, Mar 2008]