CACNA1S (NM_000069) Human Tagged ORF Clone
CAT#: RG223574
- TrueORF®
CACNA1S (GFP-tagged) - Human calcium channel, voltage-dependent, L type, alpha 1S subunit (CACNA1S)
"NM_000069" in other vectors (3)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | CACNA1S |
Synonyms | CACNL1A3; Cav1.1; CCHL1A3; HOKPP; HOKPP1; hypoPP; MHS5; TTPP1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG223574 representing NM_000069
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCATCCTCACCCCAGGATGAAGGCCTGAGGAAGAAACAGCCCAAGAAGCCAGTTCCTGAGATTC TGCCAAGGCCACCCCGGGCTTTGTTCTGCCTGACCCTGGAGAACCCCCTGAGGAAGGCCTGCATCAGCAT TGTAGAATGGAAGCCCTTCGAGACGATCATCTTGCTCACCATCTTTGCCAATTGTGTGGCCCTGGCCGTG TACCTGCCCATGCCGGAAGATGACAACAACTCTCTGAACCTCGGCCTGGAGAAGCTGGAGTATTTCTTCC TCATTGTCTTCTCGATTGAAGCCGCCATGAAGATCATTGCCTACGGCTTCTTATTCCACCAGGACGCTTA CCTGCGCAGTGGCTGGAATGTGCTGGACTTCACCATTGTCTTCCTGGGGGTCTTCACCGTGATTCTGGAA CAGGTTAACGTCATCCAAAGCCACACAGCCCCAATGAGCAGCAAAGGAGCCGGCTTGGATGTCAAGGCCC TCAGAGCCTTCCGAGTGCTCAGACCCCTCCGGCTGGTGTCGGGGGTGCCTAGCCTGCAGGTGGTCCTGAA CTCCATCTTCAAGGCCATGCTCCCCCTCTTTCACATCGCCCTGCTGGTCCTCTTTATGGTCATCATCTAT GCCATCATCGGGCTGGAGCTCTTCAAGGGCAAGATGCACAAGACCTGCTACTTCATTGGTACAGATATCG TGGCCACGGTGGAGAATGAAGAGCCATCGCCCTGCGCCAGGACGGGCTCAGGGCGCCGGTGCACCATCAA TGGCAGTGAGTGCCGGGGCGGCTGCCCAGGGCCCAACCATGGCATCACCCACTTCGACAACTTCGGCTTC TCCATGCTCACCGTGTACCAGTGCATTACCATGGAGGGATGGACTGACGTCCTTTACTGGGTCAATGATG CCATCGGGAATGAGTGGCCCTGGATCTATTTTGTCACCCTCATTTTGCTGGGATCCTTCTTCATCCTCAA CCTGGTGCTGGGTGTCCTGAGTGGGGAATTCACCAAGGAGCGGGAGAAGGCCAAGTCCAGGGGAACCTTC CAGAAGCTCCGGGAGAAGCAGCAACTAGATGAGGACCTTCGGGGCTACATGAGCTGGATCACGCAGGGCG AGGTCATGGATGTTGAGGACTTCAGAGAAGGAAAACTGTCTTTGGATGAAGGTGGCTCTGACACAGAGAG CCTGTATGAAATTGCAGGCTTGAACAAAATCATCCAGTTCATCCGACATTGGAGGCAGTGGAACCGCATC TTTCGCTGGAAGTGCCATGACATCGTGAAGTCCAAGGTCTTCTATTGGCTGGTGATTCTCATCGTTGCCC TCAACACCCTGTCTATCGCCTCAGAGCACCACAACCAGCCGCACTGGCTGACCCGTTTGCAAGACATTGC CAACCGGGTGCTGCTGTCCCTCTTCACCACTGAGATGCTGATGAAGATGTACGGGCTGGGCCTGCGCCAG TACTTCATGTCTATCTTCAACCGCTTCGACTGCTTCGTGGTGTGCAGCGGTATCCTGGAGATCCTGCTGG TGGAGTCGGGCGCCATGACACCCCTGGGCATCTCCGTGCTCCGCTGCATCCGCCTCCTGAGGATCTTCAA GATCACCAAATATTGGACGTCGCTGAGCAACCTGGTGGCATCCCTGCTCAACTCCATCCGCTCCATCGCC TCCCTGCTGCTGCTGCTCTTCCTCTTCATCGTCATCTTCCGCCTCCTGGGCATGCAGCTCTTTGGGGGGA GGTATGACTTTGAAGACACAGAAGTACGGCGCAGCAACTTTGACAACTTTCCCCAAGCCCTCATCAGCGT CTTCCAGGTACTGACAGGGGAAGACTGGACCTCAATGATGTACAATGGGATCATGGCCTCGAGCGGGCCG TCCTACCCTGGCATGCTTGTGTGCATTTACTTCATCATCCTTTTCGTCTGTGGCAACTACATCCTGCTCA ATGTCTTCCTGGCCATTGCCGTGGACAACCTGGCCGAGGCGGAGAGCCTGACTTCTGCCCAGAAGGCCAA GGCTGAGGAGAAAAAACGCAGGAAGATGTCCAAGGGTCTCCCAGACAAGTCAGAAGAGGAGAAGTCAACG ATGGCCAAGAAGCTGGAGCAGAAACCCAAGGGTGAGGGCATCCCCACCACTGCCAAGCTGAAAATCGATG AGTTTGAATCTAATGTCAATGAGGTGAAGGATCCCTACCCCTCAGCCGACTTCCCAGGGGATGACGAGGA AGATGAGCCTGAGATCCCGCTGAGCCCCCGACCACGTCCCCTGGCTGAGCTGCAGCTGAAAGAGAAGGCC GTGCCCATTCCAGAAGCCAGCTCCTTCTTCATCTTCAGCCCCACCAATAAGATCCGTGTCCTGTGTCACC GCATCGTCAATGCCACCTGGTTCACCAACTTCATCCTGCTCTTCATCCTGCTCAGCAGCGCTGCACTGGC TGCGGAAGACCCCATCCGGGCTGATTCCATGAGAAATCAGATCCTTAAACACTTTGACATCGGGTTCACC TCTGTCTTCACTGTGGAGATTGTCCTCAAGATGACGACCTACGGAGCCTTCCTGCACAAGGGTTCCTTCT GCCGCAATTACTTCAACATGCTGGACCTGCTGGTGGTGGCCGTGTCCCTCATCTCCATGGGACTTGAGTC CAGTGCCATCTCCGTGGTGAAGATCCTGAGGGTGCTGAGGGTGCTCCGACCACTCAGAGCCATCAACAGA GCCAAGGGGTTGAAGCACGTGGCTAGGTGCATGTTCGTGGCCATCAGCACCATCGGGAACATCGTGCTGG TCACTACCCTCCTACAGTTCATGTTTGCCTGCATCGGCGTCCAGCTCTTCAAGGGGAAGTTCTTCAGGTG CACCGACTTGTCCAAGATGACAGAGGAGGAGTGCAGGGGCTACTACTACGTGTACAAGGACGGGGACCCC ATGCAGATAGAGCTGCGTCACCGCGAGTGGGTACACAGCGACTTCCACTTCGACAATGTGCTCTCAGCCA TGATGTCCCTCTTCACGGTCTCCACCTTCGAGGGATGGCCTCAGCTGCTGTACAAGGCCATAGACTCCAA TGCGGAGGACGTGGGTCCCATCTACAACAACCGTGTGGAGATGGCCATCTTCTTCATCATCTACATCATC CTCATTGCCTTCTTCATGATGAACATCTTTGTGGGCTTCGTCATTGTCACCTTCCAGGAGCAGGGAGAGA CTGAGTACAAGAACTGTGAGCTGGACAAGAACCAGCGCCAATGTGTACAGTATGCCCTGAAGGCCCGCCC ACTGAGGTGCTACATTCCCAAAAACCCATACCAGTACCAGGTGTGGTACATTGTCACCTCCTCCTACTTT GAATACCTGATGTTTGCCCTCATCATGCTCAACACCATCTGCCTCGGCATGCAGCACTACAACCAGTCGG AGCAGATGAACCACATCTCAGACATCCTCAATGTGGCCTTCACTATCATCTTCACCCTGGAGATGATCCT CAAGCTCATGGCCTTCAAGGCCAGGGGCTACTTTGGAAACCCCTGGAATGTGTTTGACTTCCTGATTGTC ATTGGCAGCATCATTGATGTCATCCTCAGTGAGATCGACACTTTCCTGGCCTCCAGCGGGGGACTGTATT GCCTGGGTGGAGGCTGCGGGAACGTTGACCCAGATGAGAGTGCCCGCATCTCCAGCGCCTTCTTCCGCCT GTTCCGTGTCATGAGGCTGATCAAGCTGCTGAGCCGGGCAGAAGGAGTGCGAACCCTCCTGTGGACGTTC ATCAAGTCCTTCCAGGCCCTACCCTACGTGGCTCTGCTCATCGTCATGCTCTTCTTCATCTACGCTGTCA TCGGCATGCAGATGTTTGGGAAGATCGCCTTGGTGGATGGGACCCAAATAAACCGGAACAACAACTTCCA GACCTTCCCACAAGCTGTGCTACTGCTCTTCAGGTGTGCAACAGGTGAGGCCTGGCAGGAGATCCTACTG GCCTGCAGCTATGGGAAGCTGTGTGACCCAGAGTCGGACTATGCCCCAGGGGAGGAGTACACATGTGGCA CCAACTTTGCATACTACTACTTCATCAGCTTCTACATGCTCTGTGCCTTCCTGGTCATCAACCTCTTTGT GGCTGTCATCATGGACAATTTTGACTACCTCACCCGGGACTGGTCCATCCTGGGCCCTCATCACCTGGAT GAGTTCAAGGCCATCTGGGCAGAGTATGACCCAGAGGCTAAGGGGAGGATCAAACACCTGGACGTGGTGA CCCTGCTGAGAAGGATTCAGCCCCCTCTGGGCTTTGGGAAGTTCTGCCCACATCGGGTAGCTTGTAAGCG GCTGGTGGGCATGAACATGCCCCTGAACAGCGACGGCACAGTCACCTTCAATGCCACACTCTTTGCCCTG GTCCGCACGGCACTCAAGATCAAGACGGAAGGTAACTTTGAGCAGGCCAACGAGGAGCTGAGGGCCATCA TCAAGAAGATCTGGAAGAGAACCAGCATGAAGCTCTTGGACCAGGTCATCCCTCCAATAGGAGATGATGA GGTGACAGTGGGGAAGTTCTACGCCACATTCCTCATCCAGGAGCACTTCCGGAAGTTCATGAAACGCCAA GAGGAGTATTATGGCTATCGGCCCAAGAAGGACATTGTACAGATCCAGGCAGGGCTGCGGACCATTGAGG AAGAGGCAGCCCCCGAGATCTGTCGCACGGTCTCAGGAGACCTGGCTGCTGAGGAGGAGCTGGAGAGAGC CATGGTGGAGGCTGCGATGGAGGAGGGGATATTCCGGAGGACTGGAGGCCTGTTTGGCCAGGTGGACAAC TTCCTGGAAAGGACCAACTCCCTGCCCCCTGTCATGGCCAATCAGAGACCCCTCCAGTTTGCTGAGATAG AGATGGAAGAGATGGAGTCACCTGTCTTCTTGGAGGACTTCCCACAAGATCCACGCACCAACCCCCTGGC TCGTGCCAATACCAACAATGCCAACGCCAATGTCGCCTATGCGAACAGCAACCATAGCAACAGCCATGTG TTTTCCAGTGTCCACTATGAAAGGGAGTTCCCAGAAGAGACAGAGACGCCTGCTACCAGAGGACGAGCCC TTGGCCAACCCTGCAGGTCCCTGGGACCCCACAGCAAACCCTGTGTGGAGATGCTGAAGGGACTGCTGAC CCAGAGGGCAATGCCCAGAGGCCAGGCACCTCCTGCCCCCTGCCAGTGCCCCAGGGTGGAGTCCTCCATG CCTGAGGACAGAAAGAGCTCCACACCAGGGTCTCTTCATGAGGAGACACCCCACAGCAGGAGCACCAGGG AGAATACTTCCAGGTGCTCAGCACCAGCTACAGCCCTGCTGATCCAAAAGGCTCTGGTTCGAGGGGGCCT GGGCACCTTGGCAGCTGATGCAAACTTCATCATGGCAACAGGCCAGGCCCTCGGAGATGCCTGCCAAATG GAACCAGAGGAAGTGGAGATCATGGCAACAGAGCTACTGAAAGGACGAGAGGCCCCAGACGGCATGGCCA GCTCCCTGGGATGCCTGAACCTCGGGTCCTCCCTGGGCAGCCTCGACCAACACCAGGGCTCCCAGGAGAC CCTTATTCCTCCAAGGCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223574 representing NM_000069
Red=Cloning site Green=Tags(s) MEPSSPQDEGLRKKQPKKPVPEILPRPPRALFCLTLENPLRKACISIVEWKPFETIILLTIFANCVALAV YLPMPEDDNNSLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFTIVFLGVFTVILE QVNVIQSHTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIY AIIGLELFKGKMHKTCYFIGTDIVATVENEEPSPCARTGSGRRCTINGSECRGGCPGPNHGITHFDNFGF SMLTVYQCITMEGWTDVLYWVNDAIGNEWPWIYFVTLILLGSFFILNLVLGVLSGEFTKEREKAKSRGTF QKLREKQQLDEDLRGYMSWITQGEVMDVEDFREGKLSLDEGGSDTESLYEIAGLNKIIQFIRHWRQWNRI FRWKCHDIVKSKVFYWLVILIVALNTLSIASEHHNQPHWLTRLQDIANRVLLSLFTTEMLMKMYGLGLRQ YFMSIFNRFDCFVVCSGILEILLVESGAMTPLGISVLRCIRLLRIFKITKYWTSLSNLVASLLNSIRSIA SLLLLLFLFIVIFRLLGMQLFGGRYDFEDTEVRRSNFDNFPQALISVFQVLTGEDWTSMMYNGIMASSGP SYPGMLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEEKKRRKMSKGLPDKSEEEKST MAKKLEQKPKGEGIPTTAKLKIDEFESNVNEVKDPYPSADFPGDDEEDEPEIPLSPRPRPLAELQLKEKA VPIPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILKHFDIGFT SVFTVEIVLKMTTYGAFLHKGSFCRNYFNMLDLLVVAVSLISMGLESSAISVVKILRVLRVLRPLRAINR AKGLKHVARCMFVAISTIGNIVLVTTLLQFMFACIGVQLFKGKFFRCTDLSKMTEEECRGYYYVYKDGDP MQIELRHREWVHSDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNAEDVGPIYNNRVEMAIFFIIYII LIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIPKNPYQYQVWYIVTSSYF EYLMFALIMLNTICLGMQHYNQSEQMNHISDILNVAFTIIFTLEMILKLMAFKARGYFGNPWNVFDFLIV IGSIIDVILSEIDTFLASSGGLYCLGGGCGNVDPDESARISSAFFRLFRVMRLIKLLSRAEGVRTLLWTF IKSFQALPYVALLIVMLFFIYAVIGMQMFGKIALVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILL ACSYGKLCDPESDYAPGEEYTCGTNFAYYYFISFYMLCAFLVINLFVAVIMDNFDYLTRDWSILGPHHLD EFKAIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVGMNMPLNSDGTVTFNATLFAL VRTALKIKTEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPPIGDDEVTVGKFYATFLIQEHFRKFMKRQ EEYYGYRPKKDIVQIQAGLRTIEEEAAPEICRTVSGDLAAEEELERAMVEAAMEEGIFRRTGGLFGQVDN FLERTNSLPPVMANQRPLQFAEIEMEEMESPVFLEDFPQDPRTNPLARANTNNANANVAYANSNHSNSHV FSSVHYEREFPEETETPATRGRALGQPCRSLGPHSKPCVEMLKGLLTQRAMPRGQAPPAPCQCPRVESSM PEDRKSSTPGSLHEETPHSRSTRENTSRCSAPATALLIQKALVRGGLGTLAADANFIMATGQALGDACQM EPEEVEIMATELLKGREAPDGMASSLGCLNLGSSLGSLDQHQGSQETLIPPRL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_000069 |
ORF Size | 5619 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000069.1, NP_000060.1 |
RefSeq Size | 6160 bp |
RefSeq ORF | 5622 bp |
Locus ID | 779 |
Cytogenetics | 1q32.1 |
Protein Families | Druggable Genome, Ion Channels: Calcium, Transmembrane |
Protein Pathways | Alzheimer's disease, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Calcium signaling pathway, Cardiac muscle contraction, Dilated cardiomyopathy, GnRH signaling pathway, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Vascular smooth muscle contraction |
Gene Summary | 'This gene encodes one of the five subunits of the slowly inactivating L-type voltage-dependent calcium channel in skeletal muscle cells. Mutations in this gene have been associated with hypokalemic periodic paralysis, thyrotoxic periodic paralysis and malignant hyperthermia susceptibility. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC300005 | CACNA1S (untagged)-Human calcium channel, voltage-dependent, L type, alpha 1S subunit (CACNA1S) |
USD 2,820.00 |
|
RC223574 | CACNA1S (Myc-DDK-tagged)-Human calcium channel, voltage-dependent, L type, alpha 1S subunit (CACNA1S) |
USD 1,490.00 |
|
RC223574L1 | Lenti ORF clone of Human calcium channel, voltage-dependent, L type, alpha 1S subunit (CACNA1S), Myc-DDK-tagged |
USD 1,690.00 |
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