CACNA1S (NM_000069) Human Tagged ORF Clone

CAT#: RG223574

CACNA1S (GFP-tagged) - Human calcium channel, voltage-dependent, L type, alpha 1S subunit (CACNA1S)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CACNA1S
• Synonyms: CACNL1A3; Cav1.1; CCHL1A3; HOKPP; HOKPP1; hypoPP; MHS5; TTPP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223574 representing NM_000069
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCATCCTCACCCCAGGATGAAGGCCTGAGGAAGAAACAGCCCAAGAAGCCAGTTCCTGAGATTC
  TGCCAAGGCCACCCCGGGCTTTGTTCTGCCTGACCCTGGAGAACCCCCTGAGGAAGGCCTGCATCAGCAT
  TGTAGAATGGAAGCCCTTCGAGACGATCATCTTGCTCACCATCTTTGCCAATTGTGTGGCCCTGGCCGTG
  TACCTGCCCATGCCGGAAGATGACAACAACTCTCTGAACCTCGGCCTGGAGAAGCTGGAGTATTTCTTCC
  TCATTGTCTTCTCGATTGAAGCCGCCATGAAGATCATTGCCTACGGCTTCTTATTCCACCAGGACGCTTA
  CCTGCGCAGTGGCTGGAATGTGCTGGACTTCACCATTGTCTTCCTGGGGGTCTTCACCGTGATTCTGGAA
  CAGGTTAACGTCATCCAAAGCCACACAGCCCCAATGAGCAGCAAAGGAGCCGGCTTGGATGTCAAGGCCC
  TCAGAGCCTTCCGAGTGCTCAGACCCCTCCGGCTGGTGTCGGGGGTGCCTAGCCTGCAGGTGGTCCTGAA
  CTCCATCTTCAAGGCCATGCTCCCCCTCTTTCACATCGCCCTGCTGGTCCTCTTTATGGTCATCATCTAT
  GCCATCATCGGGCTGGAGCTCTTCAAGGGCAAGATGCACAAGACCTGCTACTTCATTGGTACAGATATCG
  TGGCCACGGTGGAGAATGAAGAGCCATCGCCCTGCGCCAGGACGGGCTCAGGGCGCCGGTGCACCATCAA
  TGGCAGTGAGTGCCGGGGCGGCTGCCCAGGGCCCAACCATGGCATCACCCACTTCGACAACTTCGGCTTC
  TCCATGCTCACCGTGTACCAGTGCATTACCATGGAGGGATGGACTGACGTCCTTTACTGGGTCAATGATG
  CCATCGGGAATGAGTGGCCCTGGATCTATTTTGTCACCCTCATTTTGCTGGGATCCTTCTTCATCCTCAA
  CCTGGTGCTGGGTGTCCTGAGTGGGGAATTCACCAAGGAGCGGGAGAAGGCCAAGTCCAGGGGAACCTTC
  CAGAAGCTCCGGGAGAAGCAGCAACTAGATGAGGACCTTCGGGGCTACATGAGCTGGATCACGCAGGGCG
  AGGTCATGGATGTTGAGGACTTCAGAGAAGGAAAACTGTCTTTGGATGAAGGTGGCTCTGACACAGAGAG
  CCTGTATGAAATTGCAGGCTTGAACAAAATCATCCAGTTCATCCGACATTGGAGGCAGTGGAACCGCATC
  TTTCGCTGGAAGTGCCATGACATCGTGAAGTCCAAGGTCTTCTATTGGCTGGTGATTCTCATCGTTGCCC
  TCAACACCCTGTCTATCGCCTCAGAGCACCACAACCAGCCGCACTGGCTGACCCGTTTGCAAGACATTGC
  CAACCGGGTGCTGCTGTCCCTCTTCACCACTGAGATGCTGATGAAGATGTACGGGCTGGGCCTGCGCCAG
  TACTTCATGTCTATCTTCAACCGCTTCGACTGCTTCGTGGTGTGCAGCGGTATCCTGGAGATCCTGCTGG
  TGGAGTCGGGCGCCATGACACCCCTGGGCATCTCCGTGCTCCGCTGCATCCGCCTCCTGAGGATCTTCAA
  GATCACCAAATATTGGACGTCGCTGAGCAACCTGGTGGCATCCCTGCTCAACTCCATCCGCTCCATCGCC
  TCCCTGCTGCTGCTGCTCTTCCTCTTCATCGTCATCTTCCGCCTCCTGGGCATGCAGCTCTTTGGGGGGA
  GGTATGACTTTGAAGACACAGAAGTACGGCGCAGCAACTTTGACAACTTTCCCCAAGCCCTCATCAGCGT
  CTTCCAGGTACTGACAGGGGAAGACTGGACCTCAATGATGTACAATGGGATCATGGCCTCGAGCGGGCCG
  TCCTACCCTGGCATGCTTGTGTGCATTTACTTCATCATCCTTTTCGTCTGTGGCAACTACATCCTGCTCA
  ATGTCTTCCTGGCCATTGCCGTGGACAACCTGGCCGAGGCGGAGAGCCTGACTTCTGCCCAGAAGGCCAA
  GGCTGAGGAGAAAAAACGCAGGAAGATGTCCAAGGGTCTCCCAGACAAGTCAGAAGAGGAGAAGTCAACG
  ATGGCCAAGAAGCTGGAGCAGAAACCCAAGGGTGAGGGCATCCCCACCACTGCCAAGCTGAAAATCGATG
  AGTTTGAATCTAATGTCAATGAGGTGAAGGATCCCTACCCCTCAGCCGACTTCCCAGGGGATGACGAGGA
  AGATGAGCCTGAGATCCCGCTGAGCCCCCGACCACGTCCCCTGGCTGAGCTGCAGCTGAAAGAGAAGGCC
  GTGCCCATTCCAGAAGCCAGCTCCTTCTTCATCTTCAGCCCCACCAATAAGATCCGTGTCCTGTGTCACC
  GCATCGTCAATGCCACCTGGTTCACCAACTTCATCCTGCTCTTCATCCTGCTCAGCAGCGCTGCACTGGC
  TGCGGAAGACCCCATCCGGGCTGATTCCATGAGAAATCAGATCCTTAAACACTTTGACATCGGGTTCACC
  TCTGTCTTCACTGTGGAGATTGTCCTCAAGATGACGACCTACGGAGCCTTCCTGCACAAGGGTTCCTTCT
  GCCGCAATTACTTCAACATGCTGGACCTGCTGGTGGTGGCCGTGTCCCTCATCTCCATGGGACTTGAGTC
  CAGTGCCATCTCCGTGGTGAAGATCCTGAGGGTGCTGAGGGTGCTCCGACCACTCAGAGCCATCAACAGA
  GCCAAGGGGTTGAAGCACGTGGCTAGGTGCATGTTCGTGGCCATCAGCACCATCGGGAACATCGTGCTGG
  TCACTACCCTCCTACAGTTCATGTTTGCCTGCATCGGCGTCCAGCTCTTCAAGGGGAAGTTCTTCAGGTG
  CACCGACTTGTCCAAGATGACAGAGGAGGAGTGCAGGGGCTACTACTACGTGTACAAGGACGGGGACCCC
  ATGCAGATAGAGCTGCGTCACCGCGAGTGGGTACACAGCGACTTCCACTTCGACAATGTGCTCTCAGCCA
  TGATGTCCCTCTTCACGGTCTCCACCTTCGAGGGATGGCCTCAGCTGCTGTACAAGGCCATAGACTCCAA
  TGCGGAGGACGTGGGTCCCATCTACAACAACCGTGTGGAGATGGCCATCTTCTTCATCATCTACATCATC
  CTCATTGCCTTCTTCATGATGAACATCTTTGTGGGCTTCGTCATTGTCACCTTCCAGGAGCAGGGAGAGA
  CTGAGTACAAGAACTGTGAGCTGGACAAGAACCAGCGCCAATGTGTACAGTATGCCCTGAAGGCCCGCCC
  ACTGAGGTGCTACATTCCCAAAAACCCATACCAGTACCAGGTGTGGTACATTGTCACCTCCTCCTACTTT
  GAATACCTGATGTTTGCCCTCATCATGCTCAACACCATCTGCCTCGGCATGCAGCACTACAACCAGTCGG
  AGCAGATGAACCACATCTCAGACATCCTCAATGTGGCCTTCACTATCATCTTCACCCTGGAGATGATCCT
  CAAGCTCATGGCCTTCAAGGCCAGGGGCTACTTTGGAAACCCCTGGAATGTGTTTGACTTCCTGATTGTC
  ATTGGCAGCATCATTGATGTCATCCTCAGTGAGATCGACACTTTCCTGGCCTCCAGCGGGGGACTGTATT
  GCCTGGGTGGAGGCTGCGGGAACGTTGACCCAGATGAGAGTGCCCGCATCTCCAGCGCCTTCTTCCGCCT
  GTTCCGTGTCATGAGGCTGATCAAGCTGCTGAGCCGGGCAGAAGGAGTGCGAACCCTCCTGTGGACGTTC
  ATCAAGTCCTTCCAGGCCCTACCCTACGTGGCTCTGCTCATCGTCATGCTCTTCTTCATCTACGCTGTCA
  TCGGCATGCAGATGTTTGGGAAGATCGCCTTGGTGGATGGGACCCAAATAAACCGGAACAACAACTTCCA
  GACCTTCCCACAAGCTGTGCTACTGCTCTTCAGGTGTGCAACAGGTGAGGCCTGGCAGGAGATCCTACTG
  GCCTGCAGCTATGGGAAGCTGTGTGACCCAGAGTCGGACTATGCCCCAGGGGAGGAGTACACATGTGGCA
  CCAACTTTGCATACTACTACTTCATCAGCTTCTACATGCTCTGTGCCTTCCTGGTCATCAACCTCTTTGT
  GGCTGTCATCATGGACAATTTTGACTACCTCACCCGGGACTGGTCCATCCTGGGCCCTCATCACCTGGAT
  GAGTTCAAGGCCATCTGGGCAGAGTATGACCCAGAGGCTAAGGGGAGGATCAAACACCTGGACGTGGTGA
  CCCTGCTGAGAAGGATTCAGCCCCCTCTGGGCTTTGGGAAGTTCTGCCCACATCGGGTAGCTTGTAAGCG
  GCTGGTGGGCATGAACATGCCCCTGAACAGCGACGGCACAGTCACCTTCAATGCCACACTCTTTGCCCTG
  GTCCGCACGGCACTCAAGATCAAGACGGAAGGTAACTTTGAGCAGGCCAACGAGGAGCTGAGGGCCATCA
  TCAAGAAGATCTGGAAGAGAACCAGCATGAAGCTCTTGGACCAGGTCATCCCTCCAATAGGAGATGATGA
  GGTGACAGTGGGGAAGTTCTACGCCACATTCCTCATCCAGGAGCACTTCCGGAAGTTCATGAAACGCCAA
  GAGGAGTATTATGGCTATCGGCCCAAGAAGGACATTGTACAGATCCAGGCAGGGCTGCGGACCATTGAGG
  AAGAGGCAGCCCCCGAGATCTGTCGCACGGTCTCAGGAGACCTGGCTGCTGAGGAGGAGCTGGAGAGAGC
  CATGGTGGAGGCTGCGATGGAGGAGGGGATATTCCGGAGGACTGGAGGCCTGTTTGGCCAGGTGGACAAC
  TTCCTGGAAAGGACCAACTCCCTGCCCCCTGTCATGGCCAATCAGAGACCCCTCCAGTTTGCTGAGATAG
  AGATGGAAGAGATGGAGTCACCTGTCTTCTTGGAGGACTTCCCACAAGATCCACGCACCAACCCCCTGGC
  TCGTGCCAATACCAACAATGCCAACGCCAATGTCGCCTATGCGAACAGCAACCATAGCAACAGCCATGTG
  TTTTCCAGTGTCCACTATGAAAGGGAGTTCCCAGAAGAGACAGAGACGCCTGCTACCAGAGGACGAGCCC
  TTGGCCAACCCTGCAGGTCCCTGGGACCCCACAGCAAACCCTGTGTGGAGATGCTGAAGGGACTGCTGAC
  CCAGAGGGCAATGCCCAGAGGCCAGGCACCTCCTGCCCCCTGCCAGTGCCCCAGGGTGGAGTCCTCCATG
  CCTGAGGACAGAAAGAGCTCCACACCAGGGTCTCTTCATGAGGAGACACCCCACAGCAGGAGCACCAGGG
  AGAATACTTCCAGGTGCTCAGCACCAGCTACAGCCCTGCTGATCCAAAAGGCTCTGGTTCGAGGGGGCCT
  GGGCACCTTGGCAGCTGATGCAAACTTCATCATGGCAACAGGCCAGGCCCTCGGAGATGCCTGCCAAATG
  GAACCAGAGGAAGTGGAGATCATGGCAACAGAGCTACTGAAAGGACGAGAGGCCCCAGACGGCATGGCCA
  GCTCCCTGGGATGCCTGAACCTCGGGTCCTCCCTGGGCAGCCTCGACCAACACCAGGGCTCCCAGGAGAC
  CCTTATTCCTCCAAGGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223574 representing NM_000069
  Red=Cloning site Green=Tags(s)
  
  MEPSSPQDEGLRKKQPKKPVPEILPRPPRALFCLTLENPLRKACISIVEWKPFETIILLTIFANCVALAV
  YLPMPEDDNNSLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFTIVFLGVFTVILE
  QVNVIQSHTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIY
  AIIGLELFKGKMHKTCYFIGTDIVATVENEEPSPCARTGSGRRCTINGSECRGGCPGPNHGITHFDNFGF
  SMLTVYQCITMEGWTDVLYWVNDAIGNEWPWIYFVTLILLGSFFILNLVLGVLSGEFTKEREKAKSRGTF
  QKLREKQQLDEDLRGYMSWITQGEVMDVEDFREGKLSLDEGGSDTESLYEIAGLNKIIQFIRHWRQWNRI
  FRWKCHDIVKSKVFYWLVILIVALNTLSIASEHHNQPHWLTRLQDIANRVLLSLFTTEMLMKMYGLGLRQ
  YFMSIFNRFDCFVVCSGILEILLVESGAMTPLGISVLRCIRLLRIFKITKYWTSLSNLVASLLNSIRSIA
  SLLLLLFLFIVIFRLLGMQLFGGRYDFEDTEVRRSNFDNFPQALISVFQVLTGEDWTSMMYNGIMASSGP
  SYPGMLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEEKKRRKMSKGLPDKSEEEKST
  MAKKLEQKPKGEGIPTTAKLKIDEFESNVNEVKDPYPSADFPGDDEEDEPEIPLSPRPRPLAELQLKEKA
  VPIPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILKHFDIGFT
  SVFTVEIVLKMTTYGAFLHKGSFCRNYFNMLDLLVVAVSLISMGLESSAISVVKILRVLRVLRPLRAINR
  AKGLKHVARCMFVAISTIGNIVLVTTLLQFMFACIGVQLFKGKFFRCTDLSKMTEEECRGYYYVYKDGDP
  MQIELRHREWVHSDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNAEDVGPIYNNRVEMAIFFIIYII
  LIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIPKNPYQYQVWYIVTSSYF
  EYLMFALIMLNTICLGMQHYNQSEQMNHISDILNVAFTIIFTLEMILKLMAFKARGYFGNPWNVFDFLIV
  IGSIIDVILSEIDTFLASSGGLYCLGGGCGNVDPDESARISSAFFRLFRVMRLIKLLSRAEGVRTLLWTF
  IKSFQALPYVALLIVMLFFIYAVIGMQMFGKIALVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILL
  ACSYGKLCDPESDYAPGEEYTCGTNFAYYYFISFYMLCAFLVINLFVAVIMDNFDYLTRDWSILGPHHLD
  EFKAIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVGMNMPLNSDGTVTFNATLFAL
  VRTALKIKTEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPPIGDDEVTVGKFYATFLIQEHFRKFMKRQ
  EEYYGYRPKKDIVQIQAGLRTIEEEAAPEICRTVSGDLAAEEELERAMVEAAMEEGIFRRTGGLFGQVDN
  FLERTNSLPPVMANQRPLQFAEIEMEEMESPVFLEDFPQDPRTNPLARANTNNANANVAYANSNHSNSHV
  FSSVHYEREFPEETETPATRGRALGQPCRSLGPHSKPCVEMLKGLLTQRAMPRGQAPPAPCQCPRVESSM
  PEDRKSSTPGSLHEETPHSRSTRENTSRCSAPATALLIQKALVRGGLGTLAADANFIMATGQALGDACQM
  EPEEVEIMATELLKGREAPDGMASSLGCLNLGSSLGSLDQHQGSQETLIPPRL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000069
• ORF Size: 5619 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000069.1, NP_000060.1
• RefSeq Size: 6160 bp
• RefSeq ORF: 5622 bp
• Locus ID: 779
• Cytogenetics: 1q32.1
• Protein Families: Druggable Genome, Ion Channels: Calcium, Transmembrane
• Protein Pathways: Alzheimer's disease, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Calcium signaling pathway, Cardiac muscle contraction, Dilated cardiomyopathy, GnRH signaling pathway, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Vascular smooth muscle contraction
• Gene Summary: 'This gene encodes one of the five subunits of the slowly inactivating L-type voltage-dependent calcium channel in skeletal muscle cells. Mutations in this gene have been associated with hypokalemic periodic paralysis, thyrotoxic periodic paralysis and malignant hyperthermia susceptibility. [provided by RefSeq, Jul 2008]'