INPP5B (NM_005540) Human Tagged ORF Clone

CAT#: RG223630

INPP5B (GFP-tagged) - Human inositol polyphosphate-5-phosphatase, 75kDa (INPP5B), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: INPP5B
• Synonyms: 5PTase
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223630 representing NM_005540
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCAGTCTGTGGCAATCCAGGAGACGCTGGCTGAGGGGGAATACTGCGTCATCGCGGTGCAAGGTG
  TGCTGTGTGAGGGGGACAGCCGGCAGAGCCGCCTCCTGGGACTCGTGCGCTACCGCCTGGAGCACGGCGG
  CCAGGAACACGCTCTCTTCCTCTATACGCACCGGAGGATGGCCATTACCGGGGACGATGTCTCTCTGGAC
  CAGATAGTGCCAGTCTCGCGGGATTTTACGCTGGAAGAAGTGTCCCCAGATGGTGAACTCTACATCCTTG
  GCTCAGATGTGACCGTCCAGCTGGACACAGCAGAGCTTAGCCTCGTATTCCAACTGCCCTTTGGTTCACA
  AACCAGGATGTTCCTCCACGAAGTTGCCAGGGCCTGTCCAGGCTTCGATTCTGCGACCCGGGATCCTGAA
  TTCCTGTGGCTGTCTCGGTATAGGTGCGCAGAGCTGGAGCTGGAGATGCCAACGCCGCGCGGTTGTAACT
  CGGCCCTAGTTACCTGGCCAGGGTACGCGACAATTGGCGGAGGTGGTTCTAACTTTGATGGTTTGAGACC
  AAATGGGAAGGGAGTGCCTATGGACCAAAGCTCCAGGGGTCAAGATAAACCAGAAAGCTTGCAACCAAGA
  CAGAATAAATCCAAGTCCGAAATTACTGACATGGTTCGCTCCTCCACTATCACAGTGTCGGACAAGGCTC
  ATATTTTATCCATGCAGAAGTTTGGACTGCGAGATACAATTGTGAAATCACATCTACTACAGAAAGAAGA
  GGATTACACCTATATCCAGAACTTCAGGTTTTTTGCGGGAACATACAATGTAAATGGGCAGTCCCCCAAA
  GAATGCCTCCGGCTGTGGCTGAGCAATGGTATCCAGGCCCCAGATGTCTATTGTGTAGGGTTCCAGGAGC
  TTGATCTGAGTAAGGAAGCTTTTTTCTTTCACGATACCCCAAAGGAGGAAGAGTGGTTCAAAGCTGTGTC
  AGAGGGTCTTCATCCAGATGCCAAATATGCAAAGGTGAAGCTTATCCGACTGGTTGGGATTATGCTGCTG
  TTATATGTCAAACAGGAGCATGCAGCTTATATCTCAGAAGTGGAAGCCGAGACTGTGGGGACAGGAATCA
  TGGGGAGGATGGGCAACAAGGGAGGCGTGGCGATCAGGTTCCAGTTCCACAACACCAGCATCTGCGTTGT
  GAATTCTCACTTGGCAGCCCACATTGAAGAGTATGAGAGGAGGAACCAGGACTATAAGGACATTTGTTCT
  CGAATGCAGTTTTGTCAGCCTGACCCAAGCCTTCCCCCTCTCACCATCAGCAACCATGATGTGATCTTGT
  GGCTGGGGGACCTCAACTACAGGATAGAAGAGCTGGATGTGGAAAAAGTGAAAAAGCTCATCGAAGAGAA
  GGACTTTCAAATGCTGTATGCATATGATCAGCTGAAAATTCAGGTGGCCGCAAAGACTGTCTTTGAAGGC
  TTCACAGAGGGTGAGCTCACATTCCAGCCTACTTACAAGTATGATACGGGCTCTGACGACTGGGATACCA
  GTGAGAAGTGCCGTGCTCCTGCCTGGTGTGATCGGATTCTCTGGAAAGGGAAGAACATCACTCAGCTGAG
  TTACCAGAGCCACATGGCCCTGAAGACCAGTGACCACAAGCCTGTCAGCTCAGTGTTTGACATCGGGGTG
  AGGGTCGTAAATGACGAGCTTTACCGGAAGACACTGGAGGAAATTGTTCGCTCCCTGGATAAGATGGAAA
  ATGCCAACATTCCTTCTGTGTCCCTGTCCAAGCGAGAGTTCTGTTTTCAGAATGTGAAGTACATGCAATT
  GAAAGTAGAATCCTTTACAATTCATAATGGACAAGTACCCTGTCATTTTGAATTCATCAACAAGCCTGAT
  GAAGAGTCTTACTGTAAGCAGTGGCTGAATGCCAACCCCAGCAGAGGCTTCCTCCTGCCAGATTCTGATG
  TTGAGATTGACTTGGAGCTCTTCGTAAATAAGATGACAGCTACAAAGCTCAACTCGGGTGAAGACAAAAT
  TGAGGACATTCTGGTTCTGCACTTGGACAGGGGAAAGGATTACTTTTTGTCTGTGTCTGGGAACTACCTG
  CCCAGCTGTTTTGGGTCTCCCATTCATACACTGTGTTACATGAGAGAGCCAATCTTGGACCTACCACTTG
  AAACCATTAGTGAGCTGACTCTGATGCCAGTATGGACTGGAGATGATGGGAGCCAGTTGGATAGCCCCAT
  GGAAATCCCCAAAGAGCTCTGGATGATGGTTGATTACCTGTACCGAAATGCTGTCCAGCAGGAAGATCTG
  TTTCAGCAACCAGGCCTGAGGTCAGAATTTGAACATATCAGGGACTGCTTGGATACTGGAATGATTGATA
  ACCTCTCTGCCAGCAATCATTCTGTAGCCGAAGCCCTGCTGCTTTTCCTGGAGAGCCTTCCAGAGCCTGT
  CATCTGTTACAGCACCTACCATAACTGCTTGGAGTGTTCTGGCAACTACACAGCAAGCAAACAGGTCATT
  TCTACTCTCCCCATATTCCACAAAAATGTCTTCCACTACTTGATGGCGTTTTTGCGAGAACTGCTGAAAA
  ATTCAGCAAAAAATCATTTGGATGAGAATATTCTAGCTAGCATATTTGGCAGCTTATTGCTTCGAAACCC
  AGCTGGTCACCAAAAGCTTGATATGACAGAGAAGAAGAAGGCTCAAGAATTTATTCACCAGTTCCTCTGC
  AACCCACTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005540
• ORF Size: 2739 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005540.2, NP_005531.2
• RefSeq Size: 4469 bp
• RefSeq ORF: 2742 bp
• Locus ID: 3633
• Cytogenetics: 1p34.3
• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: 'This gene encodes a member of a family of inositol polyphosphate-5-phosphatases. These enzymes function in the regulation of calcium signaling by inactivating inositol phosphates. The encoded protein is localized to the cytosol and mitochondria, and associates with membranes through an isoprenyl modification near the C-terminus. Alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Jul 2014]'