Alanyl tRNA synthetase 2 (AARS2) (NM_020745) Human Tagged ORF Clone

CAT#: RG223715

AARS2 (GFP-tagged) - Human alanyl-tRNA synthetase 2, mitochondrial (putative) (AARS2), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AARS2
• Synonyms: AARSL; COXPD8; LKENP; MT-ALARS; MTALARS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223715 representing NM_020745
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCGTCAGTGGCAGCTGCAGCCCGGAGGCTGCGGCGGGCCATTCGAAGGTCGCCCGCATGGCGGG
  GCCTCAGCCATCGGCCGCTCTCATCGGAGCCCCCTGCAGCCAAGGCCTCGGCCGTGAGGGCCGCCTTTCT
  GAACTTCTTTCGGGACCGCCATGGCCACCGGCTGGTGCCCTCCGCTTCCGTGCGGCCCCGCGGCGACCCC
  AGTTTGCTTTTTGTCAATGCGGGCATGAACCAGTTCAAGCCAATCTTTCTGGGCACCGTGGATCCACGAA
  GCGAGATGGCAGGCTTCCGACGTGTGGCCAACAGCCAGAAATGTGTGAGAGCTGGAGGACACCATAACGA
  CCTGGAAGATGTGGGTCGAGACCTTTCCCATCATACCTTCTTTGAAATGCTTGGCAATTGGGCCTTTGGG
  GGTGAATATTTTAAGGAGGAGGCTTGTAACATGGCCTGGGAACTGCTGACTCAGGTCTATGGGATCCCTG
  AGGAAAGGCTCTGGATCTCCTACTTTGATGGTGACCCCAAGGCAGGGCTGGACCCAGACCTGGAGACCAG
  GGACATCTGGCTGAGCTTAGGGGTGCCTGCTAGCCGTGTGCTTTCCTTTGGACCACAAGAGAACTTCTGG
  GAGATGGGGGATACTGGCCCTTGTGGGCCCTGTACTGAGATCCACTACGACCTTGCTGGTGGGGTGGGAG
  CCCCCCAGCTGGTAGAGCTTTGGAACCTGGTCTTCATGCAACACAACAGAGAGGCAGATGGAAGCCTGCA
  GCCCCTGCCCCAGCGGCATGTGGACACAGGAATGGGCCTGGAAAGGCTGGTGGCTGTGCTGCAAGGCAAA
  CACTCCACCTATGACACTGACCTCTTTTCCCCGCTGCTCAACGCCATACAGCAGGGCTGCAGGGCACCCC
  CTTACTTGGGCCGAGTAGGGGTGGCAGACGAGGGGCGCACAGACACAGCGTACCGCGTGGTGGCTGACCA
  CATCCGCACACTCAGTGTCTGCATCTCTGATGGCGTCTTCCCTGGGATGTCAGGTCCCCCGCTGGTTCTT
  CGTCGGATCCTGCGTCGAGCTGTGCGTTTCTCCATGGAGATCTTAAAGGCACCACCTGGCTTCCTAGGCA
  GCCTGGTACCTGTAGTGGTGGAGACACTGGGAGATGCTTATCCAGAACTGCAAAGGAACTCAGCCCAGAT
  CGCCAACCTGGTGTCAGAGGACGAGGCAGCCTTCCTGGCCTCCCTGGAGCGGGGTAGGCGGATCATTGAT
  CGGACTCTGAGGACCCTGGGGCCTTCAGATATGTTCCCTGCTGAAGTGGCCTGGTCCTTGTCACTGTGTG
  GAGACCTGGGACTCCCCTTGGACATGGTAGAGCTGATGCTGGAGGAGAAAGGGGTCCAGCTAGACTCCGC
  TGGACTGGAGCGGTTGGCCCAAGAGGAGGCCCAGCACCGGGCACGGCAGGCTGAGCCAGTTCAGAAGCAG
  GGATTGTGGCTTGATGTCCATGCGCTTGGGGAGCTGCAGCGCCAAGGAGTGCCCCCAACTGACGACAGCC
  CCAAGTACAACTACTCCCTGCGACCCAGCGGAAGTTATGAGTTCGGCACCTGTGAGGCCCAGGTGTTGCA
  ACTGTATACAGAGGACGGGACAGCAGTGGCCTCCGTGGGGAAAGGCCAGCGCTGTGGCCTCCTCTTGGAC
  AGGACCAACTTCTACGCAGAACAGGGGGGCCAGGCTTCAGACCGTGGCTACCTGGTGCGGGCAGGGCAAG
  AGGACGTGCTGTTCCCAGTAGCCCGGGCCCAGGTCTGTGGAGGTTTCATCCTGCATGAGGCAGTAGCCCC
  TGAGTGCCTGCGGTTAGGGGACCAGGTGCAGCTGCATGTGGATGAGGCCTGGCGTCTAGGCTGCATGGCG
  AAGCATACGGCCACCCACCTGCTGAACTGGGCACTGAGGCAGACCCTGGGCCCTGGCACAGAGCAGCAGG
  GCTCCCATCTCAATCCTGAGCAGCTGCGCTTGGATGTGACCACCCAGACCCCATTGACCCCAGAGCAGCT
  CCGGGCAGTGGAGAACACTGTGCAGGAGGCCGTGGGGCAGGATGAGGCTGTGTACATGGAGGAGGTGCCC
  CTGGCGCTCACTGCCCAGGTCCCTGGCCTGCGCTCTCTGGATGAGGTTTACCCAGACCCTGTGCGGGTGG
  TATCAGTGGGGGTGCCCGTGGCCCATGCATTGGACCCAGCCTCCCAAGCCGCACTGCAGACCTCTGTGGA
  GCTATGCTGTGGGACGCACCTGTTACGTACTGGGGCTGTAGGGGACCTGGTTATCATCGGGGACCGCCAG
  CTTTCCAAGGGCACTACCCGCCTGCTGGCCGTCACTGGGGAGCAGGCCCAGCAGGCCCGAGAGCTAGGCC
  AGAGCCTGGCCCAGGAAGTGAAAGCGGCCACTGAGCGGCTGAGTCTGGGGAGCCGGGATGTGGCGGAGGC
  ACTGAGGCTGTCCAAGGACATAGGACGACTCATTGAAGCTGTGGAAACTGCTGTGATGCCCCAGTGGCAG
  CGGCGGGAGCTGCTGGCCACAGTGAAGATGCTGCAGCGGCGTGCCAACACTGCCATCCGTAAGCTGCAAA
  TGGGACAGGCTGCAAAGAAAACTCAGGAGCTGCTGGAGCGGCACTCGAAGGGGCCTCTGATTGTGGACAC
  AGTCTCTGCTGAGTCTCTCTCAGTGCTGGTGAAGGTGGTACGGCAGCTGTGTGAGCAGGCCCCCAGCACG
  TCTGTGCTCCTACTCAGCCCCCAGCCCATGGGGAAGGTGCTGTGTGCCTGTCAGGTGGCCCAGGGTGCCA
  TGCCCACCTTCACAGCAGAGGCCTGGGCACTGGCAGTGTGCAGCCACATGGGGGGCAAGGCGTGGGGCTC
  GCGAGTGGTGGCCCAAGGCACCGGAAGCACTACTGACCTGGAAGCTGCCCTCAGTATAGCCCAAACCTAT
  GCCCTCAGCCAGCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223715 representing NM_020745
  Red=Cloning site Green=Tags(s)
  
  MAASVAAAARRLRRAIRRSPAWRGLSHRPLSSEPPAAKASAVRAAFLNFFRDRHGHRLVPSASVRPRGDP
  SLLFVNAGMNQFKPIFLGTVDPRSEMAGFRRVANSQKCVRAGGHHNDLEDVGRDLSHHTFFEMLGNWAFG
  GEYFKEEACNMAWELLTQVYGIPEERLWISYFDGDPKAGLDPDLETRDIWLSLGVPASRVLSFGPQENFW
  EMGDTGPCGPCTEIHYDLAGGVGAPQLVELWNLVFMQHNREADGSLQPLPQRHVDTGMGLERLVAVLQGK
  HSTYDTDLFSPLLNAIQQGCRAPPYLGRVGVADEGRTDTAYRVVADHIRTLSVCISDGVFPGMSGPPLVL
  RRILRRAVRFSMEILKAPPGFLGSLVPVVVETLGDAYPELQRNSAQIANLVSEDEAAFLASLERGRRIID
  RTLRTLGPSDMFPAEVAWSLSLCGDLGLPLDMVELMLEEKGVQLDSAGLERLAQEEAQHRARQAEPVQKQ
  GLWLDVHALGELQRQGVPPTDDSPKYNYSLRPSGSYEFGTCEAQVLQLYTEDGTAVASVGKGQRCGLLLD
  RTNFYAEQGGQASDRGYLVRAGQEDVLFPVARAQVCGGFILHEAVAPECLRLGDQVQLHVDEAWRLGCMA
  KHTATHLLNWALRQTLGPGTEQQGSHLNPEQLRLDVTTQTPLTPEQLRAVENTVQEAVGQDEAVYMEEVP
  LALTAQVPGLRSLDEVYPDPVRVVSVGVPVAHALDPASQAALQTSVELCCGTHLLRTGAVGDLVIIGDRQ
  LSKGTTRLLAVTGEQAQQARELGQSLAQEVKAATERLSLGSRDVAEALRLSKDIGRLIEAVETAVMPQWQ
  RRELLATVKMLQRRANTAIRKLQMGQAAKKTQELLERHSKGPLIVDTVSAESLSVLVKVVRQLCEQAPST
  SVLLLSPQPMGKVLCACQVAQGAMPTFTAEAWALAVCSHMGGKAWGSRVVAQGTGSTTDLEAALSIAQTY
  ALSQL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020745
• ORF Size: 2955 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_020745.1, NP_065796.1
• RefSeq Size: 4096
• RefSeq ORF: 2958
• Locus ID: 57505
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• Gene Summary: The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetases play critical roles in mRNA translation by charging tRNAs with their cognate amino acids. The encoded protein is a mitochondrial enzyme that specifically aminoacylates alanyl-tRNA. Mutations in this gene are a cause of combined oxidative phosphorylation deficiency 8. [provided by RefSeq, Dec 2011]