ZNF32 (NM_006973) Human Tagged ORF Clone

CAT#: RG223840

ZNF32 (GFP-tagged) - Human zinc finger protein 32 (ZNF32), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZNF32
• Synonyms: KOX30
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223840 representing NM_006973
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGGATTTCCAACAGCTACCCTGCTGGACTGTCATGGAAGATATGCCCAGAATGTAGCGTTCTTCA
  ATGTGATGACTGAAGCCCACCACAAATATGACCACTCTGAGGCTACAGGATCCTCAAGCTGGGATATCCA
  AAATTCTTTCAGAAGAGAGAAGCTGGAACAAAAATCCCCAGATTCGAAGACACTACAGGAAGATTCACCT
  GGAGTGAGACAAAGGGTCTATGAGTGCCAGGAGTGTGGAAAATCCTTCCGGCAAAAAGGTAGTCTAACGT
  TACATGAGAGAATCCACACTGGTCAAAAGCCTTTTGAGTGCACCCACTGTGGAAAAAGCTTCAGGGCCAA
  AGGCAATCTTGTTACACATCAACGGATACACACGGGAGAGAAGCCTTATCAGTGCAAGGAGTGTGGGAAA
  AGCTTCAGTCAACGAGGTAGTCTCGCTGTCCACGAGAGACTCCACACTGGACAGAAACCCTACGAGTGTG
  CTATTTGTCAGAGAAGCTTCAGGAATCAGAGTAACCTTGCTGTTCACAGGAGAGTTCACAGTGGTGAGAA
  GCCCTATAGATGTGATCAGTGTGGAAAAGCCTTCAGTCAGAAAGGAAGCTTAATTGTTCACATCAGAGTC
  CACACAGGCCTGAAGCCCTATGCCTGTACCCAGTGCAGGAAGAGTTTCCACACCAGGGGGAATTGTATTC
  TGCATGGCAAAATCCACACAGGAGAGACACCCTATCTGTGCGGCCAGTGTGGAAAAAGCTTCACCCAGAG
  AGGGAGTCTGGCTGTGCACCAGCGAAGCTGCTCACAGAGGCTCACCCTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223840 representing NM_006973
  Red=Cloning site Green=Tags(s)
  
  MFGFPTATLLDCHGRYAQNVAFFNVMTEAHHKYDHSEATGSSSWDIQNSFRREKLEQKSPDSKTLQEDSP
  GVRQRVYECQECGKSFRQKGSLTLHERIHTGQKPFECTHCGKSFRAKGNLVTHQRIHTGEKPYQCKECGK
  SFSQRGSLAVHERLHTGQKPYECAICQRSFRNQSNLAVHRRVHSGEKPYRCDQCGKAFSQKGSLIVHIRV
  HTGLKPYACTQCRKSFHTRGNCILHGKIHTGETPYLCGQCGKSFTQRGSLAVHQRSCSQRLTL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006973
• ORF Size: 819 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006973.2, NP_008904.1
• RefSeq Size: 1176 bp
• RefSeq ORF: 822 bp
• Locus ID: 7580
• Cytogenetics: 10q11.21
• Domains: zf-C2H2
• Protein Families: Transcription Factors
• Gene Summary: ''