PSD93 (DLG2) (NM_001364) Human Tagged ORF Clone

CAT#: RG223915

DLG2 (GFP-tagged) - Human discs, large homolog 2 (Drosophila) (DLG2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DLG2
• Synonyms: chapsyn-110; PPP1R58; PSD-93; PSD93
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223915 representing NM_001364
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTTTGCATGTTACTGTGCACTCCGGACTAACGTGAAGAAGTATCGATATCAAGATGAGGACGCTC
  CACATGATCATTCCTTACCTCGACTAACCCACGAAGTAAGAGGCCCAGAACTCGTGCATGTATCAGAAAA
  GAACCTCTCTCAAATAGAAAATGTCCATGGATATGTCTTGCAGTCTCATATTTCTCCTCTGAAGGCCAGT
  CCTGCTCCTATAATTGTCAACACAGATACTTTGGACACAATTCCTTATGTCAATGGGACAGAAATTGAAT
  ATGAATTTGAAGAAATTACACTGGAGAGGGGGAATTCTGGCCTGGGATTCAGTATTGCTGGGGGGACAGA
  TAATCCCCACATTGGAGATGACCCTGGCATATTTATTACGAAGATTATACCAGGAGGTGCTGCAGCAGAG
  GATGGCAGACTCAGGGTCAATGATTGTATCTTGCGGGTGAATGAGGTTGATGTGTCAGAGGTTTCCCACA
  GTAAAGCGGTGGAAGCCCTGAAGGAAGCAGGGTCTATCGTTCGGCTGTATGTGCGTAGAAGACGACCTAT
  TTTGGAGACCGTTGTGGAAATCAAACTGTTCAAAGGCCCTAAAGGTTTAGGCTTCAGTATTGCAGGAGGT
  GTGGGGAACCAACACATTCCTGGAGACAACAGCATTTATGTAACTAAAATTATAGATGGAGGAGCTGCAC
  AAAAAGATGGAAGGTTGCAAGTAGGAGATAGACTACTAATGGTAAACAACTACAGTTTAGAAGAAGTAAC
  ACACGAAGAGGCAGTAGCAATATTAAAGAACACATCAGAGGTAGTTTATTTAAAAGTTGGCAAACCCACT
  ACCATTTATATGACTGATCCTTATGGTCCACCTGATATTACTCACTCTTATTCTCCACCAATGGAAAACC
  ATCTACTCTCTGGCAACAATGGCACTTTAGAATATAAAACCTCCCTGCCACCCATCTCTCCAGGAAGGTA
  CTCACCAATTCCAAAGCACATGCTTGTTGACGACGACTACACCAGGCCTCCGGAACCTGTTTACAGCACT
  GTGAACAAACTATGTGATAAGCCTGCTTCTCCCAGGCACTATTCCCCTGTTGAGTGTGACAAAAGCTTCC
  TCCTCTCAGCTCCCTATTCCCACTACCACCTAGGCCTGCTACCTGACTCTGAGATGACCAGTCATTCCCA
  ACATAGCACCGCAACTCGTCAGCCTTCAATGACTCTCCAACGGGCCGTCTCCCTGGAAGGAGAGCCTCGC
  AAGGTAGTCCTGCACAAAGGCTCCACTGGCCTGGGCTTCAACATTGTCGGTGGGGAAGATGGAGAAGGTA
  TTTTTGTGTCCTTCATTCTGGCTGGTGGACCAGCAGACCTAAGTGGGGAGCTCCAGAGAGGAGACCAGAT
  CCTATCGGTGAATGGCATTGACCTCCGTGGTGCATCCCACGAGCAGGCAGCTGCTGCACTAAAGGGGGCT
  GGACAGACAGTGACGATTATAGCACAATATCAACCTGAAGATTACGCTCGATTTGAGGCCAAAATCCATG
  ACCTACGAGAGCAGATGATGAACCACAGCATGAGCTCCGGGTCCGGATCCCTGCGAACCAATCAGAAACG
  CTCCCTCTACGTCAGAGCCATGTTCGACTACGACAAGAGCAAGGACAGTGGGCTGCCAAGTCAAGGACTT
  AGTTTTAAATATGGAGATATTCTCCACGTTATCAATGCCTCTGATGATGAGTGGTGGCAAGCCAGGAGAG
  TCATGCTGGAGGGAGACAGTGAGGAGATGGGGGTCATCCCCAGCAAAAGGAGGGTGGAAAGAAAGGAACG
  TGCCCGATTGAAGACAGTGAAGTTTAATGCCAAACCTGGAGTGATTGATTCGAAAGGGTCATTCAATGAC
  AAGCGTAAAAAGAGCTTCATCTTTTCACGAAAATTCCCATTCTACAAGAACAAGGAGCAGAGTGAGCAGG
  AAACCAGTGATCCTGAACGAGGACAAGAAGACCTCATTCTTTCCTATGAGCCTGTTACAAGGCAGGAAAT
  AAACTACACCCGGCCGGTGATTATCCTGGGGCCCATGAAGGATCGGATCAATGACGACTTGATATCTGAA
  TTCCCTGATAAATTTGGCTCCTGTGTGCCTCATACTACGAGGCCAAAGCGAGACTACGAGGTGGATGGCA
  GAGACTATCACTTTGTCATTTCCAGAGAACAAATGGAGAAAGATATCCAAGAGCACAAGTTTATAGAAGC
  CGGCCAGTACAATGACAATTTATATGGAACCAGTGTGCAGTCTGTGAGATTTGTAGCAGAAAGAGGCAAA
  CACTGTATACTTGATGTATCAGGAAATGCTATCAAGCGGTTACAAGTTGCCCAGCTCTATCCCATTGCCA
  TCTTCATAAAACCCAGGTCTCTGGAACCTCTTATGGAGATGAATAAGCGTCTAACAGAGGAACAAGCCAA
  GAAAACCTATGATCGAGCAATTAAGCTAGAACAAGAATTTGGAGAATATTTTACAGCTATTGTCCAAGGA
  GATACTTTAGAAGATATATATAACCAATGCAAGCTTGTTATTGAAGAGCAATCTGGGCCTTTCATCTGGA
  TTCCCTCAAAGGAAAAGTTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223915 representing NM_001364
  Red=Cloning site Green=Tags(s)
  
  MFFACYCALRTNVKKYRYQDEDAPHDHSLPRLTHEVRGPELVHVSEKNLSQIENVHGYVLQSHISPLKAS
  PAPIIVNTDTLDTIPYVNGTEIEYEFEEITLERGNSGLGFSIAGGTDNPHIGDDPGIFITKIIPGGAAAE
  DGRLRVNDCILRVNEVDVSEVSHSKAVEALKEAGSIVRLYVRRRRPILETVVEIKLFKGPKGLGFSIAGG
  VGNQHIPGDNSIYVTKIIDGGAAQKDGRLQVGDRLLMVNNYSLEEVTHEEAVAILKNTSEVVYLKVGKPT
  TIYMTDPYGPPDITHSYSPPMENHLLSGNNGTLEYKTSLPPISPGRYSPIPKHMLVDDDYTRPPEPVYST
  VNKLCDKPASPRHYSPVECDKSFLLSAPYSHYHLGLLPDSEMTSHSQHSTATRQPSMTLQRAVSLEGEPR
  KVVLHKGSTGLGFNIVGGEDGEGIFVSFILAGGPADLSGELQRGDQILSVNGIDLRGASHEQAAAALKGA
  GQTVTIIAQYQPEDYARFEAKIHDLREQMMNHSMSSGSGSLRTNQKRSLYVRAMFDYDKSKDSGLPSQGL
  SFKYGDILHVINASDDEWWQARRVMLEGDSEEMGVIPSKRRVERKERARLKTVKFNAKPGVIDSKGSFND
  KRKKSFIFSRKFPFYKNKEQSEQETSDPERGQEDLILSYEPVTRQEINYTRPVIILGPMKDRINDDLISE
  FPDKFGSCVPHTTRPKRDYEVDGRDYHFVISREQMEKDIQEHKFIEAGQYNDNLYGTSVQSVRFVAERGK
  HCILDVSGNAIKRLQVAQLYPIAIFIKPRSLEPLMEMNKRLTEEQAKKTYDRAIKLEQEFGEYFTAIVQG
  DTLEDIYNQCKLVIEEQSGPFIWIPSKEKL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001364
• ORF Size: 2610 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001364.3, NP_001355.2
• RefSeq Size: 7737 bp
• RefSeq ORF: 2613 bp
• Locus ID: 1740
• Cytogenetics: 11q14.1
• Domains: SH3, PDZ, Guanylate_kin, GuKc
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. The encoded protein forms a heterodimer with a related family member that may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described, but their full-length nature is not known. [provided by RefSeq, Dec 2008]'