NT5C1B (NM_033253) Human Tagged ORF Clone

CAT#: RG224105

NT5C1B (GFP-tagged) - Human 5'-nucleotidase, cytosolic IB (NT5C1B), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NT5C1B
• Synonyms: AIRP; CN-IB; CN1B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224105 representing NM_033253
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCAAACATCTCTCAAACAGAAAAAGAATGAGCCTGGAATGAGGTCCTCAAAAGAGAGTCTAGAAG
  CAGAAAAAAGAAAGGAATCTGACAAAACAGGAGTTCGTCTGAGCAATCAGGGATCACAAGAATCATCACT
  GCGGAAGACAGACTCTCGAGGGTACCTTGTGCGCAGTCAATGGTCTAGAATATCCCGGAGCCCATCCACC
  AAGGCTCCATCCATAGATGAGCCTAGAAGCAGGAACACCAGTGCTAAGCTCCCCAGCAGCTCCACGAGCT
  CCCGGACTCCATCCACCTCCCCAAGCCTGCATGACTCCTCACCGCCGCCGCTGTCCGGGCAGCCCTCGCT
  CCAGCCACCCGCGTCGCCCCAGCTGCCCCGGTCGCTGGACTCGCGGCCTCCCACGCCCCCAGAGCCCGAT
  CCTGGCTCCCGGCGCAGCACCAAAATGCAAGAGAATCCGGAGGCCTGGGCCCAAGGCATCGTGCGGGAAA
  TCCGCCAGACCCGGGACTCGCAGCCGCTGGAATATTCGCGCACGTCCCCCACCGAGTGGAAGTCCTCCAG
  CCAGCGCAGGGGGATCTACCCCGCCTCCACCCAGCTGGACCGCAACTCTCTGTCCGAGCAGCAGCAGCAG
  CAGCGGGAGGACGAGGACGACTACGAGGCTGCCTACTGGGCATCCATGAGGTCGTTCTACGAGAAGAACC
  CGAGCTGCTCGCGCCCCTGGCCGCCCAAACCCAAGAACGCCATCACCATTGCTCTCTCATCCTGCGCGCT
  CTTCAACATGGTGGACGGCAGGAAAATCTACGAGCAAGAGGGTCTGGAAAAGTACATGGAGTATCAGCTC
  ACCAATGAGAACGTCATCCTGACCCCGGGCCCGGCGTTCCGCTTCGTCAAGGCACTACAGTATGTCAATG
  CTAGACTCCGTGATCTATATCCTGATGAACAGGACTTATTTGATATTGTACTGATGACTAATAACCATGC
  CCAAGTGGGAGTGCGGCTTATAAACAGCGTCAATCACTACGGCTTACTGATTGACCGCTTCTGTCTGACC
  GGGGGAAAAGACCCCATTGGCTATTTGAAGGCATATCTTACCAACTTGTATATTGCTGCAGATTCTGAAA
  AAGTGCAAGAGGCAATACAAGAAGGTATTGCCTCTGCGACAATGTTTGATGGAGCCAAAGACATGGCTTA
  CTGTGACACTCAGCTCCGTGTAGCCTTTGATGGGGATGCTGTCCTCTTCTCTGATGAGTCTGAACATTTT
  ACCAAGGAGCATGGGCTCGACAAATTCTTCCAGTATGATACATTATGTGAAAGTAAGCCTCTTGCTCAGG
  GTCCCCTAAAAGGCTTTCTGGAAGATTTAGGCAGACTGCAAAAGAAGTTCTATGCCAAAAATGAACGGTT
  ACTTTGTCCTATCAGGACCTACCTGGTTACAGCTAGGAGTGCAGCCAGTTCAGGCGCCCGTGTGCTGAAG
  ACCCTTCGACGCTGGGGTCTAGAGATAGACGAAGCTCTTTTCCTTGCTGGAGCCCCCAAAAGTCCCATCT
  TGGTGAAGATCCGGCCCCACATCTTCTTTGATGACCACATGTTCCACATTGAAGGGGCACAGAGGTTAGG
  TTCCATCGCAGCTTATGGCTTTAATAAAAAATTCAGTAGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224105 representing NM_033253
  Red=Cloning site Green=Tags(s)
  
  MSQTSLKQKKNEPGMRSSKESLEAEKRKESDKTGVRLSNQGSQESSLRKTDSRGYLVRSQWSRISRSPST
  KAPSIDEPRSRNTSAKLPSSSTSSRTPSTSPSLHDSSPPPLSGQPSLQPPASPQLPRSLDSRPPTPPEPD
  PGSRRSTKMQENPEAWAQGIVREIRQTRDSQPLEYSRTSPTEWKSSSQRRGIYPASTQLDRNSLSEQQQQ
  QREDEDDYEAAYWASMRSFYEKNPSCSRPWPPKPKNAITIALSSCALFNMVDGRKIYEQEGLEKYMEYQL
  TNENVILTPGPAFRFVKALQYVNARLRDLYPDEQDLFDIVLMTNNHAQVGVRLINSVNHYGLLIDRFCLT
  GGKDPIGYLKAYLTNLYIAADSEKVQEAIQEGIASATMFDGAKDMAYCDTQLRVAFDGDAVLFSDESEHF
  TKEHGLDKFFQYDTLCESKPLAQGPLKGFLEDLGRLQKKFYAKNERLLCPIRTYLVTARSAASSGARVLK
  TLRRWGLEIDEALFLAGAPKSPILVKIRPHIFFDDHMFHIEGAQRLGSIAAYGFNKKFSS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033253
• ORF Size: 1650 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_033253.2, NP_150278.2
• RefSeq Size: 2684
• RefSeq ORF: 1653
• Locus ID: 93034
• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism, Purine metabolism, Pyrimidine metabolism
• Gene Summary: Cytosolic 5-prime nucleotidases, such as NT5C1B, catalyze production of adenosine, which regulates diverse physiologic processes (Sala-Newby and Newby, 2001 [PubMed 11690631]). [supplied by OMIM, Mar 2008]