TRIM37 (NM_015294) Human Tagged ORF Clone

CAT#: RG224302

TRIM37 (GFP-tagged) - Human tripartite motif containing 37 (TRIM37), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TRIM37
• Synonyms: MUL; POB1; TEF3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224302 representing NM_015294
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGAACAGAGCGTGGAGAGCATTGCTGAGGTTTTCCGATGTTTCATTTGTATGGAGAAATTGCGGG
  ATGCACGCCTGTGTCCTCATTGCTCCAAACTGTGTTGTTTCAGCTGTATTAGGCGCTGGCTGACAGAGCA
  GAGAGCTCAATGTCCTCATTGCCGTGCTCCACTCCAGCTACGAGAACTAGTAAATTGTCGTTGGGCAGAA
  GAAGTAACACAACAGCTTGATACTCTTCAACTCTGCAGTCTCACCAAACATGAAGAAAATGAAAAGGACA
  AATGTGAAAATCACCATGAAAAACTTAGTGTATTTTGCTGGGCTTGTAAGAAGTGTATCTGCCATCAGTG
  TGCACTTTGGGGAGGAATGCATGGCGGACATACCTTTAAACCTTTGGCAGAAATTTATGAGCAACACGTC
  ACTAAAGTGAATGAAGAGGTAGCCAAACTTCGTCGGCGTCTCATGGAACTGATCAGCTTAGTTCAAGAAG
  TGGAAAGGAATGTAGAAGCTGTAAGAAATGCAAAAGATGAGCGTGTTCGGGAAATTAGGAATGCAGTGGA
  GATGATGATTGCACGGTTAGACACACAGCTGAAGAATAAGCTTATAACACTGATGGGTCAGAAGACATCT
  CTAACCCAAGAAACAGAGCTTTTGGAATCCTTACTTCAGGAGGTGGAGCACCAGTTGCGGTCTTGTAGTA
  AGAGTGAGTTGATATCTAAGAGCTCAGAGATCCTTATGATGTTTCAGCAAGTTCATCGGAAGCCCATGGC
  ATCTTTTGTTACCACTCCTGTTCCACCAGACTTTACCAGTGAATTAGTGCCATCTTACGATTCAGCTACT
  TTTGTTTTAGAGAATTTCAGCACTTTGCGTCAGAGAGCAGATCCTGTTTACAGTCCACCTCTTCAAGTTT
  CAGGACTTTGCTGGAGGTTAAAAGTTTACCCAGATGGAAATGGAGTTGTGCGAGGTTACTACTTATCTGT
  GTTTCTGGAGCTCTCAGCTGGCTTGCCTGAAACTTCTAAATATGAATATCGTGTAGAGATGGTTCACCAG
  TCCTGTAATGATCCTACAAAAAATATCATTCGAGAATTTGCATCTGACTTTGAAGTTGGAGAATGCTGGG
  GCTATAATAGATTTTTCCGTTTGGACTTACTCGCAAATGAAGGATACTTGAATCCACAAAATGATACAGT
  GATTTTAAGGTTTCAGGTACGTTCACCAACTTTCTTTCAAAAATCCCGGGACCAGCATTGGTACATTACT
  CAGCTGGAAGCTGCACAGACTAGTTATATCCAACAAATAAACAACCTTAAAGAGAGACTTACTATTGAGC
  TGTCTCGAACTCAGAAGTCAAGAGATTTGTCACCACCAGATAACCATCTTAGCCCCCAAAATGATGATGC
  TCTGGAGACACGAGCTAAGAAGTCTGCATGCTCTGACATGCTTCTCGAAGGTGGTCCTACTACAGCTTCT
  GTAAGAGAGGCCAAAGAGGATGAAGAAGATGAGGAGAAGATTCAGAATGAAGATTATCATCACGAGCTTT
  CAGATGGAGATCTGGATCTGGATCTTGTTTATGAGGATGAAGTAAATCAGCTCGATGGCAGCAGTTCCTC
  TGCTAGTTCCACAGCAACAAGTAATACAGAAGAAAATGATATTGATGAAGAAACTATGTCTGGAGAAAAT
  GATGTGGAATATAACAACATGGAATTAGAAGAGGGAGAACTCATGGAAGATGCAGCTGCTGCAGGACCCG
  CAGGTAGTAGCCATGGTTATGTGGGTTCCAGTAGTAGAATATCAAGAAGAACACATTTATGCTCCGCTGC
  TACCAGTAGTTTACTAGACATTGATCCATTAATTTTAATACATTTGTTGGACCTTAAGGACCGGAGCAGT
  ATAGAAAATTTGTGGGGCTTACAGCCTCGCCCACCTGCTTCACTTCTGCAGCCCACAGCATCATATTCTC
  GAAAAGATAAAGACCAAAGGAAGCAACAGGCAATGTGGCGAGTGCCCTCTGATTTAAAGATGCTAAAAAG
  ACTCAAAACTCAAATGGCCGAAGTTCGATGTATGAAAACTGATGTAAAGAATACACTTTCAGAAATAAAA
  AGCAGCAGTGCTGCTTCTGGAGACATGCAGACAAGCCTTTTTTCTGCTGACCAGGCAGCTCTGGCTGCAT
  GTGGAACTGAAAACTCTGGCAGATTGCAGGATTTGGGAATGGAACTCCTGGCAAAGTCATCAGTTGCCAA
  TTGTTACATACGAAACTCCACAAATAAGAAGAGTAATTCGCCCAAGCCAGCTCGATCCAGTGTAGCAGGT
  AGTCTATCACTTCGAAGAGCAGTGGACCCTGGAGAAAATAGTCGTTCAAAGGGAGACTGTCAGACTCTGT
  CTGAAGGCTCCCCAGGAAGCTCTCAGTCTGGGAGCAGGCACAGTTCTCCCCGAGCCTTGATACATGGCAG
  TATCGGTGATATTCTGCCAAAAACTGAAGACCGGCAGTGTAAAGCTTTGGATTCAGATGCTGTTGTGGTT
  GCAGTTTTCAGTGGCTTGCCTGCGGTTGAGAAAAGGAGGAAAATGGTCACCTTGGGGGCTAATGCTAAAG
  GAGGTCATCTGGAAGGACTGCAGATGACTGATTTGGAAAATAATTCTGAAACTGGAGAGTTACAGCCTGT
  ACTACCTGAAGGAGCTTCAGCTGCCCCTGAAGAAGGAATGAGTAGCGACAGTGACATTGAATGTGACACT
  GAGAATGAGGAGCAGGAAGAGCATACCAGTGTGGGCGGGTTTCACGACTCCTTCATGGTCATGACACAGC
  CCCCGGATGAAGATACACATTCCAGTTTTCCTGATGGTGAACAAATAGGCCCTGAAGATCTCAGCTTCAA
  TACAGATGAAAATAGTGGAAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224302 representing NM_015294
  Red=Cloning site Green=Tags(s)
  
  MDEQSVESIAEVFRCFICMEKLRDARLCPHCSKLCCFSCIRRWLTEQRAQCPHCRAPLQLRELVNCRWAE
  EVTQQLDTLQLCSLTKHEENEKDKCENHHEKLSVFCWACKKCICHQCALWGGMHGGHTFKPLAEIYEQHV
  TKVNEEVAKLRRRLMELISLVQEVERNVEAVRNAKDERVREIRNAVEMMIARLDTQLKNKLITLMGQKTS
  LTQETELLESLLQEVEHQLRSCSKSELISKSSEILMMFQQVHRKPMASFVTTPVPPDFTSELVPSYDSAT
  FVLENFSTLRQRADPVYSPPLQVSGLCWRLKVYPDGNGVVRGYYLSVFLELSAGLPETSKYEYRVEMVHQ
  SCNDPTKNIIREFASDFEVGECWGYNRFFRLDLLANEGYLNPQNDTVILRFQVRSPTFFQKSRDQHWYIT
  QLEAAQTSYIQQINNLKERLTIELSRTQKSRDLSPPDNHLSPQNDDALETRAKKSACSDMLLEGGPTTAS
  VREAKEDEEDEEKIQNEDYHHELSDGDLDLDLVYEDEVNQLDGSSSSASSTATSNTEENDIDEETMSGEN
  DVEYNNMELEEGELMEDAAAAGPAGSSHGYVGSSSRISRRTHLCSAATSSLLDIDPLILIHLLDLKDRSS
  IENLWGLQPRPPASLLQPTASYSRKDKDQRKQQAMWRVPSDLKMLKRLKTQMAEVRCMKTDVKNTLSEIK
  SSSAASGDMQTSLFSADQAALAACGTENSGRLQDLGMELLAKSSVANCYIRNSTNKKSNSPKPARSSVAG
  SLSLRRAVDPGENSRSKGDCQTLSEGSPGSSQSGSRHSSPRALIHGSIGDILPKTEDRQCKALDSDAVVV
  AVFSGLPAVEKRRKMVTLGANAKGGHLEGLQMTDLENNSETGELQPVLPEGASAAPEEGMSSDSDIECDT
  ENEEQEEHTSVGGFHDSFMVMTQPPDEDTHSSFPDGEQIGPEDLSFNTDENSGR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015294
• ORF Size: 2892 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015294.2, NP_056109.1
• RefSeq Size: 4488 bp
• RefSeq ORF: 2895 bp
• Locus ID: 4591
• Cytogenetics: 17q22
• Domains: zf-B_box, MATH, BBC
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: 'This gene encodes a member of the tripartite motif (TRIM) family, whose members are involved in diverse cellular functions such as developmental patterning and oncogenesis. The TRIM motif includes zinc-binding domains, a RING finger region, a B-box motif and a coiled-coil domain. The RING finger and B-box domains chelate zinc and might be involved in protein-protein and/or protein-nucleic acid interactions. Mutations in this gene are associated with mulibrey (muscle-liver-brain-eye) nanism, an autosomal recessive disorder that involves several tissues of mesodermal origin. TRIM37 localizes in peroxisomal membranes, and has been implicated in human peroxisomal biogenesis disorders. [provided by RefSeq, Jul 2020]'