TCTN1 (NM_001082538) Human Tagged ORF Clone

CAT#: RG224477

TCTN1 (GFP-tagged) - Human tectonic family member 1 (TCTN1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TCTN1
• Synonyms: JBTS13; TECT1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224477 representing NM_001082538
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCCGCGAGGTCTCCCGCCGCTCCTGGTGGTGCTCCTGGGCTGCTGGGCCTCCGTGAGCGCCCAGA
  CCGATGCCACCCCGGCGGTGACGACAGAGGGCCTCAACTCCACCGAGGCAGCCCTGGCCACCTTCGGAAC
  TTTCCCGTCGACCAGGCCCCCCGGGACTCCCAGGGCTCCAGGGCCCTCCTCCGGCCCCAGGCCTACCCCA
  GTCACGGACGTTGCTGTTCTCTGTGTCTGTGACTTATCCCCAGCACAGTGTGACATCAACTGCTGCTGTG
  ATCCCGACTGCAGCTCCGTGGATTTCAGTGTCTTTTCTGCCTGCTCAGTTCCAGTTGTCACGGGCGACAG
  CCAGTTTTGTAGTCAAAAAGCAGTCATCTATTCATTGAATTTTACAGCAAACCCACCTCAAAGAGTATTT
  GAACTTGTTGACCAGATTAATCCATCTATTTTCTGCATTCATATTACAAACTATAAACCTGCATTATCCT
  TTATTAATCCAGAAGTACCTGATGAAAACAATTTTGATACATTGATGAAAACATCTGATGGTTTTACATT
  GAATGCTGAATCATATGTTTCCTTCACAACCAAACTGGATATTCCTACTGCTGCTAAATATGAGTATGGG
  GTTCCTCTGCAGACTTCAGATTCGTTTCTGAGATTTCCTTCGTCCCTGACATCATCTCTGTGCACTGATA
  ATAACCCTGCAGCGTTTCTGGTGAACCAGGCTGTTAAGTGCACCAGAAAAATAAATTTAGAACAGTGTGA
  AGAAATTGAAGCCCTCAGCATGGCTTTTTACAGCAGCCCGGAAATTCTGAGGGTACCTGATTCAAGAAAA
  AAGGTCCCTATCACTGTTCAGTCCATCGTCATTCAGTCTCTAAATAAAACGCTCACCCGACGGGAGGACA
  CTGATGTGCTGCAGCCGACTCTCGTCAACGCTGGACACTTTAGCCTTTGCGTGAATGTTGTTCTTGAGGT
  AAAGTACAGCCTCACATACACAGATGCAGGTGAAGTCACCAAAGCTGATCTCTCATTCGTTCTGGGGACA
  GTTAGCAGCGTAGTGGTCCCACTGCAGCAAAAGTTTGAAATTCATTTTCTTCAGGAAAATACCCAGCCAG
  TCCCTCTCAGTGGAAACCCTGGTTATGTCGTGGGGCTCCCATTAGCTGCTGGATTCCAGCCTCATAAGGG
  GTCTGGGATTATTCAGACCACAAATAGATATGGACAGCTTACTATTCTTCATAGCACAACTGAGCAAGAC
  TGCTTAGCACTGGAGGGGGTCCGGACCCCAGTATTATTTGGTTACACTATGCAATCTGGCTGTAAACTAA
  GACTGACTGGAGCTCTCCCGTGTCAGCTCGTAGCACAGAAGGTGAAGAGCCTGCTGTGGGGCCAGGGCTT
  CCCAGATTACGTGGCCCCTTTTGGAAATTCCCAGGCCCAGGACATGCTGGACTGGGTGCCCATCCACTTC
  ATCACCCAGTCATTCAACAGGAAGCATTTTGTTTTGCAGGATTCCTGCCAGCTCCCAGGGGCTTTGGTTA
  TAGAAGTGAAGTGGACTAAATACGGATCCCTGCTGAATCCACAGGCCAAAATAGTCAATGTAACTGCAAA
  TCTAATTTCATCCTCCTTTCCTGAGGCCAACTCAGGAAATGAAAGGACGATTCTTATTTCCACTGCGGTT
  ACTTTTGTGGATGTGTCTGCACCTGCAGAGGCAGGCTTCAGAGCTCCACCAGCCATCAATGCCAGGCTGC
  CCTTTAACTTCTTCTTCCCGTTTGTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224477 representing NM_001082538
  Red=Cloning site Green=Tags(s)
  
  MRPRGLPPLLVVLLGCWASVSAQTDATPAVTTEGLNSTEAALATFGTFPSTRPPGTPRAPGPSSGPRPTP
  VTDVAVLCVCDLSPAQCDINCCCDPDCSSVDFSVFSACSVPVVTGDSQFCSQKAVIYSLNFTANPPQRVF
  ELVDQINPSIFCIHITNYKPALSFINPEVPDENNFDTLMKTSDGFTLNAESYVSFTTKLDIPTAAKYEYG
  VPLQTSDSFLRFPSSLTSSLCTDNNPAAFLVNQAVKCTRKINLEQCEEIEALSMAFYSSPEILRVPDSRK
  KVPITVQSIVIQSLNKTLTRREDTDVLQPTLVNAGHFSLCVNVVLEVKYSLTYTDAGEVTKADLSFVLGT
  VSSVVVPLQQKFEIHFLQENTQPVPLSGNPGYVVGLPLAAGFQPHKGSGIIQTTNRYGQLTILHSTTEQD
  CLALEGVRTPVLFGYTMQSGCKLRLTGALPCQLVAQKVKSLLWGQGFPDYVAPFGNSQAQDMLDWVPIHF
  ITQSFNRKHFVLQDSCQLPGALVIEVKWTKYGSLLNPQAKIVNVTANLISSSFPEANSGNERTILISTAV
  TFVDVSAPAEAGFRAPPAINARLPFNFFFPFV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001082538
• ORF Size: 1776 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001082538.1, NP_001076007.1
• RefSeq Size: 1992
• RefSeq ORF: 1779
• Locus ID: 79600
• Protein Families: Secreted Protein
• Gene Summary: This gene encodes a member of a family of secreted and transmembrane proteins. The orthologous gene in mouse functions downstream of smoothened and rab23 to modulate hedgehog signal transduction. This protein is a component of the tectonic-like complex, which forms a barrier between the ciliary axoneme and the basal body. A mutation in this gene was found in a family with Joubert syndrome-13. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]