GTF2A1 (NM_015859) Human Tagged ORF Clone
CAT#: RG224564
- TrueORF®
GTF2A1 (GFP-tagged) - Human general transcription factor IIA, 1, 19/37kDa (GTF2A1), transcript variant 1
"NM_015859" in other vectors (4)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | GTF2A1 |
Synonyms | TF2A1; TFIIA; TFIIA-42; TFIIAL |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG224564 representing NM_015859
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAACTCGGCAAATACAAACACCGTGCCTAAATTATACAGATCTGTGATTGAAGATGTCATTAATG ATGTGAGAGACATCTTTCTGGATGATGGAGTGGATGAACAAGTACTGATGGAACTAAAAACTTTATGGGA AAACAAACTAATGCAGTCCAGGGCAGTAGATGGATTTCATTCAGAAGAGCAGCAGCTTCTACTGCAAGTT CAACAGCAGCATCAACCCCAGCAGCAGCAGCATCACCACCATCACCATCATCAGCAAGCTCAGCCTCAGC AGACAGTACCTCAGCAAGCGCAGACCCAGCAGGTTCTTATTCCTGCATCACAGCAAGCCACAGCACCACA AGTTATTGTTCCAGATTCTAAGTTGATACAGCATATGAATGCATCAAACATGAGTGCTGCTGCTACAGCT GCTACCTTAGCACTCCCTGCAGGTGTGACTCCTGTTCAGCAGATATTAACAAATTCAGGCCAGCTTCTTC AGGTGGTCAGAGCAGCCAATGGTGCCCAATATATCTTTCAGCCTCAGCAGTCAGTGGTTCTACAACAACA GGTTATACCACAAATGCAGCCTGGTGGAGTACAAGCTCCTGTTATACAGCAGGTGCTGGCTCCTCTTCCT GGAGGGATTTCACCACAGACAGGTGTCATCATCCAGCCTCAGCAAATCTTATTTACAGGAAATAAGACTC AAGTTATACCTACGACAGTGGCAGCACCTACACCAGCCCAAGCACAGATAACTGCAACTGGCCAGCAGCA ACCGCAGGCCCAGCCTGCTCAAACACAAGCTCCATTGGTCTTACAAGTTGATGGAACTGGGGATACATCA TCTGAAGAAGATGAAGATGAAGAAGAAGACTATGATGATGATGAGGAGGAAGACAAAGAGAAAGATGGAG CTGAAGATGGGCAGGTGGAAGAAGAGCCCCTCAATAGTGAAGATGATGTGAGTGATGAGGAAGGACAGGA ACTCTTTGACACAGAAAATGTTGTTGTATGCCAATATGATAAGATACACAGAAGTAAAAACAAATGGAAA TTTCATCTCAAGGATGGCATTATGAATCTTAATGGAAGAGATTATATATTTTCCAAAGCCATTGGAGATG CAGAATGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG224564 representing NM_015859
Red=Cloning site Green=Tags(s) MANSANTNTVPKLYRSVIEDVINDVRDIFLDDGVDEQVLMELKTLWENKLMQSRAVDGFHSEEQQLLLQV QQQHQPQQQQHHHHHHHQQAQPQQTVPQQAQTQQVLIPASQQATAPQVIVPDSKLIQHMNASNMSAAATA ATLALPAGVTPVQQILTNSGQLLQVVRAANGAQYIFQPQQSVVLQQQVIPQMQPGGVQAPVIQQVLAPLP GGISPQTGVIIQPQQILFTGNKTQVIPTTVAAPTPAQAQITATGQQQPQAQPAQTQAPLVLQVDGTGDTS SEEDEDEEEDYDDDEEEDKEKDGAEDGQVEEEPLNSEDDVSDEEGQELFDTENVVVCQYDKIHRSKNKWK FHLKDGIMNLNGRDYIFSKAIGDAEW TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_015859 |
ORF Size | 1128 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_015859.3, NP_056943.1 |
RefSeq Size | 6336 bp |
RefSeq ORF | 1131 bp |
Locus ID | 2957 |
Cytogenetics | 14q31.1 |
Domains | TFIIA |
Protein Families | Transcription Factors |
Protein Pathways | Basal transcription factors |
Gene Summary | 'Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.[supplied by OMIM, Jul 2010]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC310468 | GTF2A1 (untagged)-Human general transcription factor IIA, 1, 19/37kDa (GTF2A1), transcript variant 1 |
USD 760.00 |
|
RC224564 | GTF2A1 (Myc-DDK-tagged)-Human general transcription factor IIA, 1, 19/37kDa (GTF2A1), transcript variant 1 |
USD 420.00 |
|
RC224564L3 | Lenti-ORF clone of GTF2A1 (Myc-DDK-tagged)-Human general transcription factor IIA, 1, 19/37kDa (GTF2A1), transcript variant 1 |
USD 620.00 |
|
RC224564L4 | Lenti-ORF clone of GTF2A1 (mGFP-tagged)-Human general transcription factor IIA, 1, 19/37kDa (GTF2A1), transcript variant 1 |
USD 620.00 |
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