MECR (NM_016011) Human Tagged ORF Clone

CAT#: RG224634

MECR (GFP-tagged) - Human mitochondrial trans-2-enoyl-CoA reductase (MECR), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MECR
• Synonyms: CGI-63; DYTOABG; ETR1; FASN2B; NRBF1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224634 representing NM_016011
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGGTCTGCAGTACCCTGTGGCGGGTGCGAACCCCCGCCCGGCAGTGGCGGGGGCTGCTCCCAGCTT
  CTGGCTGTCACGGACCTGCCGCCTCCTCCTACTCCGCATCCGCCGAGCCTGCCCGGGTCCGGGCGCTTGT
  CTATGGGCACCACGGGGATCCAGCCAAGGTCGTCGAACTCAAGAACCTGGAGCTAGCTGCTGTGAGAGGA
  TCAGATGTCCGTGTGAAGATGCTGGCGGCCCCTATCAATCCATCTGACATAAATATGATCCAAGGAAACT
  ACGGACTCCTTCCTGAACTGCCTGCTGTTGGAGGGAACGAAGGTGTTGCACAGGTGGTAGCGGTGGGCAG
  CAATGTGACCGGGCTGAAGCCAGGAGACTGGGTGATTCCAGCAAATGCTGGTTTAGGAACCTGGCGGACC
  GAGGCTGTGTTCAGCGAGGAAGCACTGATCCAAGTTCCGAGTGACATCCCTCTTCAGAGCGCTGCCACCC
  TGGGTGTCAATCCCTGCACAGCCTACAGGATGTTGATGGACTTCGAGCAACTGCAGCCAGGGGATTCTGT
  CATCCAGAATGCATCCAACAGCGGAGTGGGGCAAGCGGTCATCCAGATCGCCGCAGCCCTGGGCCTAAGA
  ACCATCAATGTGGTCCGAGACAGACCTGATATCCAGAAGCTGAGTGACAGACTGAAGAGTCTGGGGGCTG
  AGCATGTCATCACAGAAGAGGAGCTAAGAAGGCCCGAAATGAAAAACTTCTTTAAGGACATGCCCCAGCC
  ACGGCTTGCTCTCAACTGTGTTGGTGGGAAAAGCTCCACAGAGCTGCTGCGGCAGTTAGCGCGTGGAGGA
  ACCATGGTAACCTATGGGGGGATGGCCAAGCAGCCCGTCGTAGCCTCTGTGAGCCTGCTCATTTTTAAGG
  ATCTCAAACTTCGAGGCTTTTGGTTGTCCCAGTGGAAGAAGGATCACAGTCCAGACCAGTTCAAGGAGCT
  GATCCTCACACTGTGCGATCTCATCCGCCGAGGCCAGCTCACAGCCCCTGCCTGCTCCCAGGTCCCGCTG
  CAGGACTACCAGTCTGCCTTGGAAGCCTCCATGAAGCCCTTCATATCTTCAAAGCAGATTCTCACCATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224634 representing NM_016011
  Red=Cloning site Green=Tags(s)
  
  MWVCSTLWRVRTPARQWRGLLPASGCHGPAASSYSASAEPARVRALVYGHHGDPAKVVELKNLELAAVRG
  SDVRVKMLAAPINPSDINMIQGNYGLLPELPAVGGNEGVAQVVAVGSNVTGLKPGDWVIPANAGLGTWRT
  EAVFSEEALIQVPSDIPLQSAATLGVNPCTAYRMLMDFEQLQPGDSVIQNASNSGVGQAVIQIAAALGLR
  TINVVRDRPDIQKLSDRLKSLGAEHVITEEELRRPEMKNFFKDMPQPRLALNCVGGKSSTELLRQLARGG
  TMVTYGGMAKQPVVASVSLLIFKDLKLRGFWLSQWKKDHSPDQFKELILTLCDLIRRGQLTAPACSQVPL
  QDYQSALEASMKPFISSKQILTM
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016011
• ORF Size: 1119 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016011.2, NP_057095.2
• RefSeq Size: 2307
• RefSeq ORF: 1122
• Locus ID: 51102
• Domains: ADH_zinc_N
• Protein Families: Druggable Genome
• Protein Pathways: Fatty acid elongation in mitochondria, Metabolic pathways
• Gene Summary: The protein encoded by this gene is an oxidoreductase that catalyzes the last step in mitochondrial fatty acid synthesis. Defects in this gene are a cause of childhood-onset dystonia and optic atrophy. [provided by RefSeq, Mar 2017]