CTAGE5 (MIA2) (NM_203354) Human Tagged ORF Clone

CAT#: RG224768

CTAGE5 (GFP-tagged) - Human CTAGE family, member 5 (CTAGE5), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MIA2
• Synonyms: CTAGE5; MEA6; MGEA; MGEA6; MGEA11; TALI
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224768 representing NM_203354
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAATTGAAAAGTCCAGAGGAAGAGGTTGTGGCAGCACTGCCTGAAGGTATGAGACCAGATTCTAATC
  TTTATGGTTTTCCATGGGAATTGGTGATATGTGCAGCTGTTGTTGGATTTTTTGCTGTTCTCTTTTTTTT
  GTGGAGAAGTTTTAGATCGGTTAGGAGTCGGCTTTATGTGGGACGAGAGAAAAAGCTTGCTCTAATGCTT
  TCTGGACTAATTGAAGAAAAAAGTAAACTACTTGAAAAATTTAGCCTTGTTCAAAAAGAGTATGAAGGCT
  ATGAAGTAGAGTCATCTTTAAAGGATGCCAGCTTTGAGAAGGAGGCAACAGAAGCACAAAGTTTGGAGGC
  AACCTGTGAAAAGCTGAACAGGTCCAATTCTGAACTTGAGGATGAAATACTCTGTCTAGAAAAAGAGTTA
  AAAGAAGAGAAATCCAAACATTCTGAACAAGATGAATTGATGGCGGATATTTCAAAAAGGATACAGTCTC
  TAGAAGATGAGTCAAAATCCCTCAAATCACAAGTAGCTGAAGCCAAAATGACCTTCAAGATATTTCAAAT
  GAATGAAGAACGACTGAAGATAGCAATAAAAGATGCTTTGAATGAAAATTCTCAACTTCAGGAAAGCCAG
  AAACAGCTTTTGCAAGAAGCTGAAGTATGGAAAGAACAAGTGAGTGAACTTAATAAACAGAAAGTAACAT
  TTGAAGACTCCAAAGTACATGCAGAACAAGTTCTAAATGATAAAGAAAGTCACATCAAGACTCTGACTGA
  ACGCTTGTTAAAGATGAAAGATTGGGCTGCTATGCTTGGAGAAGACATAACGGATGATGATAACTTGGAA
  TTAGAAATGAACAGTGAATCGGAAAATGGTGCTTACTTAGATAATCCTCCAAAAGGAGCTTTGAAGAAAC
  TGATTCATGCTGCTAAGTTAAATGCTTCTTTAAAAACCTTAGAAGGAGAAAGAAACCAAATTTATATTCA
  GTTGTCTGAAGTTGATAAAACAAAGGAAGAGCTTACAGAGCATATTAAAAATCTTCAGACTGAACAAGCA
  TCTTTGCAGTCAGAAAACACACATTTTGAAAATGAGAATCAGAAGCTTCAACAGAAACTTAAAGTAATGA
  CTGAATTATATCAAGAAAATGAAATGAAACTCCACAGGAAATTAACAGTAGAGGAAAATTATCGGTTAGA
  GAAAGAAGAGAAACTTTCTAAAGTAGATGAAAAGATCAGCCATGCCACTGAAGAGCTGGAGACCTATAGA
  AAGCGAGCCAAAGATCTTGAAGAAGAATTGGAGAGAACTATTCATTCTTATCAAGGGCAGATTATTTCCC
  ATGAGAAAAAAGCACATGATAATTGGTTGGCAGCTCGGAATGCTGAAAGAAACCTCAATGATTTAAGGAA
  AGAAAATGCTCACAACAGACAAAAATTAACTGAAACAGAGCTTAAATTTGAACTTTTAGAAAAAGATCCT
  TATGCACTCGATGTTCCAAATACAGCATTTGGCAGAGAGCATTCCCCATATGGTCCCTCACCATTGGGTT
  GGCCTTCATCTGAAACAAGAGCTTTTCTCTCTCCTCCAACTTTGTTGGAGGGTCCACTCAGACTCTCACC
  TTTGCTTCCAGGGGGAGGAGGAAGAGGCTCACGAGGCCCAGGGAATCCTCTGGACCATCAGATTACCAAT
  GAAAGAGGAGAATCAAGCTGTGATAGGTTAACCGATCCTCATAGGGCTCCCTCTGACACTGGGTCTCTGT
  CACCTCCATGGGACCAGGACCGTAGGATGATGTTTCCTCCGCCAGGACAATCATATCCTGATTCAGCCCT
  TCCTCCACAAAGGCAAGACAGATTTTGTTCTAATTCTGGTAGACTGTCTGGACCAGCAGAACTCAGAAGT
  TTTAATATGCCTTCTTTGGATAAAATGGATGGGTCAATGCCTTCAGAAATGGAATCCAGTAGAAATGATA
  CCAAAGATGATCTTGGTAATTTAAATGTGCCTGATTCATCTCTCCCTGCTGAAAATGAAGCCACTGGCCC
  TGGCTTTGTTCCTCCACCTCTTGCTCCAATCAGAGGTCCATTGTTTCCAGTGGATGCAAGAGGCCCATTC
  TTGAGAAGAGGACCTCCTTTCCCCCCACCTCCTCCAGGAGCCATGTTTGGAGCTTCTCGAGATTATTTTC
  CACCAGGGGATTTCCCAGGTCCACCACCTGCTCCATTTGCAATGAGAAATGTCTATCCACCGAGGGGTTT
  TCCTCCTTACCTTCCCCCAAGACCTGGATTTTTCCCCCCACCCCCACATTCTGAAGGTAGAAGTGAGTTC
  CCCTCAGGTTTGATTCCACCTTCAAATGAGCCTGCTACTGAACATCCAGAACCACAGCAAGAAACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224768 representing NM_203354
  Red=Cloning site Green=Tags(s)
  
  MELKSPEEEVVAALPEGMRPDSNLYGFPWELVICAAVVGFFAVLFFLWRSFRSVRSRLYVGREKKLALML
  SGLIEEKSKLLEKFSLVQKEYEGYEVESSLKDASFEKEATEAQSLEATCEKLNRSNSELEDEILCLEKEL
  KEEKSKHSEQDELMADISKRIQSLEDESKSLKSQVAEAKMTFKIFQMNEERLKIAIKDALNENSQLQESQ
  KQLLQEAEVWKEQVSELNKQKVTFEDSKVHAEQVLNDKESHIKTLTERLLKMKDWAAMLGEDITDDDNLE
  LEMNSESENGAYLDNPPKGALKKLIHAAKLNASLKTLEGERNQIYIQLSEVDKTKEELTEHIKNLQTEQA
  SLQSENTHFENENQKLQQKLKVMTELYQENEMKLHRKLTVEENYRLEKEEKLSKVDEKISHATEELETYR
  KRAKDLEEELERTIHSYQGQIISHEKKAHDNWLAARNAERNLNDLRKENAHNRQKLTETELKFELLEKDP
  YALDVPNTAFGREHSPYGPSPLGWPSSETRAFLSPPTLLEGPLRLSPLLPGGGGRGSRGPGNPLDHQITN
  ERGESSCDRLTDPHRAPSDTGSLSPPWDQDRRMMFPPPGQSYPDSALPPQRQDRFCSNSGRLSGPAELRS
  FNMPSLDKMDGSMPSEMESSRNDTKDDLGNLNVPDSSLPAENEATGPGFVPPPLAPIRGPLFPVDARGPF
  LRRGPPFPPPPPGAMFGASRDYFPPGDFPGPPPAPFAMRNVYPPRGFPPYLPPRPGFFPPPPHSEGRSEF
  PSGLIPPSNEPATEHPEPQQET
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_203354
• ORF Size: 2376 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_203354.1, NP_976229.1
• RefSeq Size: 3605 bp
• RefSeq ORF: 2379 bp
• Locus ID: 4253
• Cytogenetics: 14q21.1
• Protein Families: Transmembrane
• Gene Summary: 'This gene encodes s receptor in the endoplasmic reticulum, which plays a role in the export of large pre-chylomicrons and pre-very low density lipoproteins (pre-VLDLs). Three major classes of transcripts are generated from this gene- melanoma inhibitory activity 2-specific transcripts, cTAGE family member 5-specific transcripts and transcripts that include exons from both these transcript species (TANGO1-like or TALI). Additionally, alternative splicing in these transcripts results in multiple transcript variants encoding multiple isoforms. [provided by RefSeq, Sep 2016]'