p57 Kip2 (CDKN1C) (NM_001122630) Human Tagged ORF Clone
CAT#: RG225427
- TrueORF®
CDKN1C (GFP-tagged) - Human cyclin-dependent kinase inhibitor 1C (p57, Kip2) (CDKN1C), transcript variant 2. Note: ORF is codon optimized
"NM_001122630" in other vectors (2)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | CDKN1C |
Synonyms | BWCR; BWS; KIP2; p57; p57Kip2; WBS |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG225427 ORF sequence, codon optimized.
Due to the complexity of NM_001122630, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAGACTGGTGGCTCGGGGTACTTTTCCAGTGCTCGTTCGGACTAGCGCATGCCGAAGCCTGTTTG GTCCTGTTGATCATGAAGAGCTCAGCCGGGAGCTCCAGGCCCGACTGGCTGAGCTGAACGCTGAAGACCA GAATCGCTGGGACTACGATTTTCAGCAAGACATGCCGTTGAGAGGCCCAGGCAGGTTGCAATGGACAGAG GTCGATTCTGATTCCGTGCCTGCCTTCTATAGGGAAACAGTACAAGTGGGACGATGCCGACTGCTGCTGG CGCCCCGCCCTGTTGCAGTGGCCGTGGCCGTGAGCCCACCGCTGGAACCCGCCGCGGAGTCCTTGGACGG TTTGGAAGAGGCACCCGAGCAGCTTCCGTCCGTCCCCGTGCCCGCCCCCGCATCAACCCCACCACCTGTT CCAGTCCTTGCTCCCGCCCCCGCACCCGCCCCAGCCCCGGTTGCAGCCCCCGTGGCCGCTCCGGTCGCAG TAGCTGTGTTGGCCCCTGCCCCAGCCCCAGCTCCAGCCCCCGCACCCGCTCCGGCCCCGGTGGCCGCGCC AGCACCCGCCCCGGCACCCGCCCCAGCCCCGGCCCCAGCCCCTGCGCCAGCGCCTGATGCCGCCCCCCAG GAGAGCGCCGAACAAGGCGCCAACCAGGGACAGAGGGGTCAGGAGCCTCTCGCTGATCAACTTCATAGCG GTATTTCTGGCCGCCCCGCCGCCGGTACAGCCGCTGCTAGTGCTAATGGTGCTGCCATCAAGAAACTGTC CGGGCCACTGATCAGCGATTTCTTCGCCAAGAGAAAAAGATCTGCTCCAGAGAAGAGTAGCGGGGACGTG CCTGCACCCTGCCCGTCCCCCTCCGCTGCCCCAGGTGTCGGCTCTGTGGAACAGACTCCTCGGAAGAGAC TTAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG225427 representing NM_001122630
Red=Cloning site Green=Tags(s) MERLVARGTFPVLVRTSACRSLFGPVDHEELSRELQARLAELNAEDQNRWDYDFQQDMPLRGPGRLQWTE VDSDSVPAFYRETVQVGRCRLLLAPRPVAVAVAVSPPLEPAAESLDGLEEAPEQLPSVPVPAPASTPPPV PVLAPAPAPAPAPVAAPVAAPVAVAVLAPAPAPAPAPAPAPAPVAAPAPAPAPAPAPAPAPAPAPDAAPQ ESAEQGANQGQRGQEPLADQLHSGISGRPAAGTAAASANGAAIKKLSGPLISDFFAKRKRSAPEKSSGDV PAPCPSPSAAPGVGSVEQTPRKRLR TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001122630 |
ORF Size | 915 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001122630.1, NP_001116102.1 |
RefSeq Size | 1776 bp |
RefSeq ORF | 918 bp |
Locus ID | 1028 |
Cytogenetics | 11p15.4 |
Protein Families | Druggable Genome |
Protein Pathways | Cell cycle |
Gene Summary | 'This gene is imprinted, with preferential expression of the maternal allele. The encoded protein is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC318824 | CDKN1C (untagged)-Human cyclin-dependent kinase inhibitor 1C (p57, Kip2) (CDKN1C), transcript variant 2 |
USD 660.00 |
|
RC225427 | CDKN1C (Myc-DDK-tagged)-Human cyclin-dependent kinase inhibitor 1C (p57, Kip2) (CDKN1C), transcript variant 2. Note: ORF is codon optimized |
USD 420.00 |
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