p57 Kip2 (CDKN1C) (NM_001122630) Human Tagged ORF Clone

CAT#: RG225427

CDKN1C (GFP-tagged) - Human cyclin-dependent kinase inhibitor 1C (p57, Kip2) (CDKN1C), transcript variant 2. Note: ORF is codon optimized

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CDKN1C
• Synonyms: BWCR; BWS; KIP2; p57; p57Kip2; WBS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG225427 ORF sequence, codon optimized.
  Due to the complexity of NM_001122630, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGACTGGTGGCTCGGGGTACTTTTCCAGTGCTCGTTCGGACTAGCGCATGCCGAAGCCTGTTTG
  GTCCTGTTGATCATGAAGAGCTCAGCCGGGAGCTCCAGGCCCGACTGGCTGAGCTGAACGCTGAAGACCA
  GAATCGCTGGGACTACGATTTTCAGCAAGACATGCCGTTGAGAGGCCCAGGCAGGTTGCAATGGACAGAG
  GTCGATTCTGATTCCGTGCCTGCCTTCTATAGGGAAACAGTACAAGTGGGACGATGCCGACTGCTGCTGG
  CGCCCCGCCCTGTTGCAGTGGCCGTGGCCGTGAGCCCACCGCTGGAACCCGCCGCGGAGTCCTTGGACGG
  TTTGGAAGAGGCACCCGAGCAGCTTCCGTCCGTCCCCGTGCCCGCCCCCGCATCAACCCCACCACCTGTT
  CCAGTCCTTGCTCCCGCCCCCGCACCCGCCCCAGCCCCGGTTGCAGCCCCCGTGGCCGCTCCGGTCGCAG
  TAGCTGTGTTGGCCCCTGCCCCAGCCCCAGCTCCAGCCCCCGCACCCGCTCCGGCCCCGGTGGCCGCGCC
  AGCACCCGCCCCGGCACCCGCCCCAGCCCCGGCCCCAGCCCCTGCGCCAGCGCCTGATGCCGCCCCCCAG
  GAGAGCGCCGAACAAGGCGCCAACCAGGGACAGAGGGGTCAGGAGCCTCTCGCTGATCAACTTCATAGCG
  GTATTTCTGGCCGCCCCGCCGCCGGTACAGCCGCTGCTAGTGCTAATGGTGCTGCCATCAAGAAACTGTC
  CGGGCCACTGATCAGCGATTTCTTCGCCAAGAGAAAAAGATCTGCTCCAGAGAAGAGTAGCGGGGACGTG
  CCTGCACCCTGCCCGTCCCCCTCCGCTGCCCCAGGTGTCGGCTCTGTGGAACAGACTCCTCGGAAGAGAC
  TTAGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG225427 representing NM_001122630
  Red=Cloning site Green=Tags(s)
  
  MERLVARGTFPVLVRTSACRSLFGPVDHEELSRELQARLAELNAEDQNRWDYDFQQDMPLRGPGRLQWTE
  VDSDSVPAFYRETVQVGRCRLLLAPRPVAVAVAVSPPLEPAAESLDGLEEAPEQLPSVPVPAPASTPPPV
  PVLAPAPAPAPAPVAAPVAAPVAVAVLAPAPAPAPAPAPAPAPVAAPAPAPAPAPAPAPAPAPAPDAAPQ
  ESAEQGANQGQRGQEPLADQLHSGISGRPAAGTAAASANGAAIKKLSGPLISDFFAKRKRSAPEKSSGDV
  PAPCPSPSAAPGVGSVEQTPRKRLR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001122630
• ORF Size: 915 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001122630.1, NP_001116102.1
• RefSeq Size: 1776 bp
• RefSeq ORF: 918 bp
• Locus ID: 1028
• Cytogenetics: 11p15.4
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle
• Gene Summary: 'This gene is imprinted, with preferential expression of the maternal allele. The encoded protein is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]'