Ephexin 1 (NGEF) (NM_001114090) Human Tagged ORF Clone

CAT#: RG226027

NGEF (GFP-tagged) - Human neuronal guanine nucleotide exchange factor (NGEF), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NGEF
• Synonyms: ARHGEF27; EPHEXIN
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226027 representing NM_001114090
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGCTGGCCGCTGCCTTCAGCGCCGCCTGCGCCGTGGACCACGACAGTTCCACCTCGGAAAGCG
  ACGCGCGCGACTCGGCGGCGGGACACCTGCCCGGCAGTGAGTCGTCCTCCACCCCGGGAAATGGGGCCAC
  GCCCGAGGAGTGGCCGGCCCTGGCCGACAGCCCCACCACGCTCACCGAGGCCCTGCGGATGATCCACCCC
  ATTCCCGCCGACTCCTGGAGAAACCTCATTGAACAAATAGGGCTCCTGTATCAGGAATACCGAGATAAAT
  CGACTCTCCAAGAAATCGAAACCAGGAGGCAACAGGATGCAGAAATAGAAGACAATACCAATGGGTCCCC
  GGCCAGTGAGGACACCCCGGAGGAGGAAGAAGAAGAGGAGGAGGAGGAGGAGCCGGCCAGCCCACCAGAG
  AGGAAGACTCTGCCCCAGATCTGCCTGCTCAGTAACCCCCACTCAAGGTTCAACCTCTGGCAGGATCTTC
  CCGAGATCCGGAGCAGCGGGGTGCTTGAGATCCTACAGCCCGAGGAGATTAAGCTGCAGGAGGCCATGTT
  CGAGCTGGTCACTTCCGAGGCGTCCTACTACAAGAGTCTGAACCTGCTCGTGTCCCACTTCATGGAGAAC
  GAGCGGATAAGGAAGATCCTGCACCCGTCCGAGGCGCACATCCTCTTCTCCAACGTCCTGGACGTGCTGG
  CTGTCAGTGAGCGGTTCCTCCTGGAGCTGGAGCACCGGATGGAGGAGAACATCGTCATCTCTGACGTGTG
  TGACATTGTGTACCGTTATGCGGCCGACCACTTCTCTGTCTACATCACCTACGTCAGCAATCAGACCTAC
  CAGGAGCGGACCTATAAGCAGCTGCTCCAGGAGAAGGCAGCTTTCCGGGAGCTGATCGCGCAGCTAGAGC
  TCGACCCCAAGTGCAGGGGGCTGCCCTTCTCCTCCTTCCTCATCCTGCCTTTCCAGAGGATCACACGCCT
  CAAGCTGTTGGTCCAGAACATCCTGAAGAGGGTAGAAGAGAGGTCTGAGCGGGAGTGCACTGCTTTGGAT
  GCTCACAAGGAGCTGGAAATGGTGGTGAAGGCATGCAACGAGGGCGTCAGGAAAATGAGCCGCACGGAAC
  AGATGATCAGCATTCAGAAGAAGATGGAGTTCAAGATCAAGTCGGTGCCCATCATCTCCCACTCCCGCTG
  GCTGCTGAAGCAGGGTGAGCTGCAGCAGATGTCAGGCCCCAAGACCTCCCGGACCCTGAGGACCAAGAAG
  CTCTTCCACGAAATTTACCTCTTCCTGTTCAACGACCTGCTGGTGATCTGCCGGCAGATTCCAGGAGACA
  AGTACCAGGTATTTGACTCAGCTCCGCGGGGACTGCTGCGTGTGGAGGAGCTGGAGGACCAGGGCCAGAC
  GCTGGCCAACGTGTTCATCCTGCGGCTGCTGGAGAACGCAGATGACCGGGAGGCCACCTACATGCTAAAG
  GCGTCCTCTCAGAGTGAGATGAAGCGTTGGATGACCTCACTGGCCCCCAACAGGAGGACCAAGTTTGTTT
  CGTTCACATCCCGGCTGCTGGACTGCCCCCAGGTCCAGTGCGTGCACCCATACGTGGCTCAGCAGCCAGA
  CGAGCTGACGCTGGAGCTCGCCGACATCCTCAACATCCTGGACAAGACTGACGACGGGTGGATCTTTGGC
  GAGCGTCTGCACGACCAGGAGAGAGGCTGGTTCCCCAGCTCCATGACTGAGGAGATCTTGAATCCCAAGA
  TCCGGTCCCAGAACCTCAAGGAATGTTTCCGTGTCCACAAGATGGATGACCCTCAGCGCAGCCAGAACAA
  GGACCGCAGGAAGCTGGGCAGCCGGAATCGGCAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226027 representing NM_001114090
  Red=Cloning site Green=Tags(s)
  
  MELLAAAFSAACAVDHDSSTSESDARDSAAGHLPGSESSSTPGNGATPEEWPALADSPTTLTEALRMIHP
  IPADSWRNLIEQIGLLYQEYRDKSTLQEIETRRQQDAEIEDNTNGSPASEDTPEEEEEEEEEEEPASPPE
  RKTLPQICLLSNPHSRFNLWQDLPEIRSSGVLEILQPEEIKLQEAMFELVTSEASYYKSLNLLVSHFMEN
  ERIRKILHPSEAHILFSNVLDVLAVSERFLLELEHRMEENIVISDVCDIVYRYAADHFSVYITYVSNQTY
  QERTYKQLLQEKAAFRELIAQLELDPKCRGLPFSSFLILPFQRITRLKLLVQNILKRVEERSERECTALD
  AHKELEMVVKACNEGVRKMSRTEQMISIQKKMEFKIKSVPIISHSRWLLKQGELQQMSGPKTSRTLRTKK
  LFHEIYLFLFNDLLVICRQIPGDKYQVFDSAPRGLLRVEELEDQGQTLANVFILRLLENADDREATYMLK
  ASSQSEMKRWMTSLAPNRRTKFVSFTSRLLDCPQVQCVHPYVAQQPDELTLELADILNILDKTDDGWIFG
  ERLHDQERGWFPSSMTEEILNPKIRSQNLKECFRVHKMDDPQRSQNKDRRKLGSRNRQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001114090
• ORF Size: 1854 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001114090.1, NP_001107562.1
• RefSeq Size: 2812
• RefSeq ORF: 1857
• Locus ID: 25791
• Protein Pathways: Axon guidance