TLE3 (NM_020908) Human Tagged ORF Clone

CAT#: RG226146

TLE3 (GFP-tagged) - Human transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila) (TLE3), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TLE3
• Synonyms: ESG; ESG3; GRG3; HsT18976
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226146 representing NM_020908
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATCCGCAGGGCAGACATCCGGCTCCCCATCAACCCGGGCAGCCGGGATTTAAATTCACGGTGGCTG
  AGTCTTGTGACAGGATCAAAGACGAATTCCAGTTCCTGCAAGCTCAGTATCACAGCCTCAAAGTGGAGTA
  CGACAAGCTGGCAAACGAGAAGACGGAGATGCAGCGCCATTATGTGATGTACTATGAGATGTCCTATGGC
  TTGAACATTGAAATGCACAAGCAGACAGAGATTGCGAAGAGACTGAACACAATTTTAGCACAGATCATGC
  CTTTCCTGTCACAAGAGCACCAGCAGCAGGTGGCGCAGGCAGTGGAGCGCGCCAAGCAGGTCACCATGAC
  GGAGCTGAACGCCATCATCGGGCAGCAGCAGCTCCAGGCGCAGCACCTCTCCCATGCCACACACGGCCCC
  CCGGTCCAGTTGCCACCCCACCCGTCAGGTCTCCAGCCTCCAGGAATCCCCCCAGTGACAGGGAGCAGCT
  CCGGGCTGCTGGCACTGGGCGCCCTGGGCAGCCAGGCCCATCTGACGGTGAAGGATGAGAAGAACCACCA
  TGAACTCGATCACAGAGAGAGAGAATCCAGTGCGAATAACTCTGTGTCACCCTCGGAAAGCCTCCGGGCC
  AGTGAGAAGCACCGGGGCTCTGCGGACTACAGCATGGAAGCCAAGAAGCGGAAGGCGGAGGAGAAGGACA
  GCTTGAGCCGATACGACAGTGATGGAGACAAGAGTGATGATCTGGTGGTGGATGTTTCCAATGAGGACCC
  CGCAACGCCCCGGGTCAGCCCGGCACACTCCCCTCCTGAAAATGGGCTGGACAAGGCCCGTAGCCTGAAA
  AAAGATGCCCCCACCAGCCCTGCCTCGGTGGCCTCTTCCAGTAGCACACCTTCCTCCAAGACCAAAGACC
  TTGGTCATAACGACAAATCCTCCACCCCTGGGCTCAAGTCCAACACACCAACCCCAAGGAACGACGCCCC
  AACTCCAGGCACCAGCACGACCCCAGGGCTCAGGTCGATGCCGGCCTCGGCTCTGCGCACGCCCATCTCC
  ATCACCAGCTCCTATGCGGCGCCCTTCGCCATGATGAGCCACCATGAGATGAACGGCTCCCTCACCAGTC
  CTGGCGCCTACGCCGGCCTCCACAACATCCCACCCCAGATGAGCGCCGCCGCCGCTGCTGCAGCCGCTGC
  CTATGGCCGATCGCCAATGGTGAGCTTTGGAGCTGTTGGTTTTGACCCTCACCCCCCGATGCGGGCCACA
  GGCCTCCCCTCAAGCCTGGCCTCCATTCCTGGAGGAAAACCAGCGTACTCATTCCATGTGAGTGCTGATG
  GGCAGATGCAGCCCGTGCCCTTCCCCCACGACGCCCTGGCAGGCCCCGGCATCCCGAGGCACGCCCGGCA
  GATCAACACACTCAGCCACGGGGAGGTGGTGTGTGCCGTGACCATCAGCAACCCCACGAGGCACGTCTAC
  ACAGGTGGCAAGGGCTGCGTGAAGATCTGGGACATCAGCCAGCCAGGCAGCAAGAGCCCCATCTCCCAGC
  TGGACTGCCTGAACAGGGACAATTACATCCGCTCCTGCAAGCTGCTCCCTGATGGGCGCACGCTCATCGT
  GGGCGGCGAGGCCAGCACGCTCACCATCTGGGACCTGGCCTCGCCCACGCCCCGCATCAAGGCCGAGCTG
  ACGTCCTCGGCTCCCGCCTGTTATGCCCTGGCCATTAGCCCTGACGCCAAAGTCTGCTTCTCCTGCTGCA
  GCGATGGGAACATTGCTGTCTGGGACCTGCACAACCAGACCCTGGTCAGGCAGTTCCAGGGCCACACAGA
  TGGGGCCAGCTGCATAGACATCTCCCATGATGGCACCAAACTGTGGACAGGGGGCCTGGACAACACGGTG
  CGCTCCTGGGACCTGCGGGAGGGCCGACAGCTACAGCAGCATGACTTCACTTCCCAGATCTTCTCGCTGG
  GCTACTGCCCCACTGGGGAGTGGCTGGCTGTGGGCATGGAGAGCAGCAACGTGGAGGTGCTGCACCACAC
  CAAGCCTGACAAGTACCAGCTGCACCTGCACGAGAGCTGCGTGCTCTCCCTCAAGTTCGCCTACTGCGGC
  AAGTGGTTCGTGAGCACTGGGAAAGATAACCTTCTCAACGCCTGGAGGACGCCTTATGGAGCCAGCATAT
  TCCAGTCTAAAGAATCCTCGTCTGTCTTGAGTTGTGACATTTCAGCGGATGACAAATACATTGTAACAGG
  CTCTGGTGACAAGAAGGCCACAGTTTATGAGGTCATCTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226146 representing NM_020908
  Red=Cloning site Green=Tags(s)
  
  MYPQGRHPAPHQPGQPGFKFTVAESCDRIKDEFQFLQAQYHSLKVEYDKLANEKTEMQRHYVMYYEMSYG
  LNIEMHKQTEIAKRLNTILAQIMPFLSQEHQQQVAQAVERAKQVTMTELNAIIGQQQLQAQHLSHATHGP
  PVQLPPHPSGLQPPGIPPVTGSSSGLLALGALGSQAHLTVKDEKNHHELDHRERESSANNSVSPSESLRA
  SEKHRGSADYSMEAKKRKAEEKDSLSRYDSDGDKSDDLVVDVSNEDPATPRVSPAHSPPENGLDKARSLK
  KDAPTSPASVASSSSTPSSKTKDLGHNDKSSTPGLKSNTPTPRNDAPTPGTSTTPGLRSMPASALRTPIS
  ITSSYAAPFAMMSHHEMNGSLTSPGAYAGLHNIPPQMSAAAAAAAAAYGRSPMVSFGAVGFDPHPPMRAT
  GLPSSLASIPGGKPAYSFHVSADGQMQPVPFPHDALAGPGIPRHARQINTLSHGEVVCAVTISNPTRHVY
  TGGKGCVKIWDISQPGSKSPISQLDCLNRDNYIRSCKLLPDGRTLIVGGEASTLTIWDLASPTPRIKAEL
  TSSAPACYALAISPDAKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTV
  RSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHHTKPDKYQLHLHESCVLSLKFAYCG
  KWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISADDKYIVTGSGDKKATVYEVIY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020908
• ORF Size: 2280 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020908.1, NP_065959.1
• RefSeq Size: 5318 bp
• RefSeq ORF: 2283 bp
• Locus ID: 7090
• Cytogenetics: 15q23
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a transcriptional co-repressor protein that belongs to the transducin-like enhancer family of proteins. The members of this family function in the Notch signaling pathway that regulates determination of cell fate during development. Expression of this gene has been associated with a favorable outcome to chemotherapy with taxanes for ovarian carcinoma. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Sep 2013]'