ARHGEF7 (NM_001113511) Human Tagged ORF Clone

CAT#: RG226174

ARHGEF7 (GFP-tagged) - Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ARHGEF7
• Synonyms: BETA-PIX; COOL-1; COOL1; Nbla10314; P50; P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226174 representing NM_001113511
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTCCGCCGAGCAAACCGTTACGTGGCTCATCACTCTGGGGGTGCTGGAGTCGCCCAAAAAAACCA
  TCTCGGACCCGGAGGGCTTTCTGCAGGCGTCGCTGAAGGATGGGGTGGTCCTCTGCAGGCTGCTGGAGCG
  CCTGCTCCCCGGGACCATCGAGAAAGTCTACCCCGAGCCCCGGAGCGAGAGCGAGTGCCTGAGCAACATC
  CGCGAGTTCCTGCGCGGCTGCGGGGCTTCCCTGCGGCTGGAGCTCCTGTTTCCTCCTTCCCAGCCTCCTC
  AGCATCTGGTAACCACCATTCTCTTGTCTGCTTCTACGTTTGATGCAAATGATTTGTATCAGGGGCAGAA
  TTTTAACAAGGTCCTCAGTTCCTTAGTGACTCTAAATAAAGTAACAGCAGACATCGGGCTGGGGAGTGAC
  TCCGTGTGTGCCCGGCCCTCGTCTCACCGCATAAAGTCTTTTGACTCCCTTGGATCACAGTCTTTGCACA
  CTCGGACTTCAAAACTGTTCCAGGGCCAGTATCGGAGTTTGGACATGACCGATAATAGCAACAATCAACT
  GGTAGTAAGAGCAAAGTTTAACTTCCAGCAGACCAATGAGGACGAGCTTTCCTTCTCAAAAGGAGACGTC
  ATCCATGTCACCCGTGTGGAAGAGGGAGGCTGGTGGGAGGGCACACTCAACGGCCGGACCGGCTGGTTCC
  CCAGCAACTACGTGCGCGAGGTCAAGGCCAGCGAGAAGCCTGTGTCTCCCAAATCAGGAACACTGAAGAG
  CCCTCCCAAAGGATTTGATACGACTGCCATAAACAAAAGCTATTACAATGTGGTGCTACAGAATATTTTA
  GAAACAGAAAATGAATATTCTAAAGAACTTCAGACTGTGCTTTCAACGTACCTACGGCCATTGCAGACCA
  GTGAGAAGTTAAGTTCAGCAAACATTTCATATTTAATGGGAAATCTAGAAGAAATATGTTCTTTCCAGCA
  AATGCTCGTACAGTCTTTAGAAGAATGCACCAAGTTGCCCGAAGCTCAGCAGAGAGTCGGAGGCTGCTTT
  TTAAACCTGATGCCACAGATGAAAACCCTGTACCTCACGTATTGTGCCAATCACCCTTCTGCAGTGAATG
  TCCTCACGGAACACAGTGAGGAGTTGGGGGAGTTCATGGAGACCAAAGGTGCCAGCAGCCCTGGGATTCT
  CGTGCTGACCACGGGCCTGAGCAAACCCTTCATGCGCCTGGATAAATACCCTACGCTGCTCAAAGAGCTC
  GAGAGACACATGGAGGATTATCATACAGATAGACAAGATATTCAAAAATCCATGGCTGCCTTCAAAAACC
  TTTCAGCCCAATGTCAAGAAGTCCGGAAGAGGAAAGAGCTTGAGCTGCAGATCCTGACGGAAGCCATCCG
  GAACTGGGAGGGCGATGACATTAAAACTCTGGGCAACGTCACTTACATGTCCCAGGTCCTGATTCAGTGT
  GCCGGAAGTGAGGAAAAGAATGAAAGATATCTTCTACTCTTCCCAAATGTTTTGCTAATGTTGTCTGCCA
  GTCCTAGGATGAGTGGCTTTATCTATCAGGGAAAGCTTCCAACGACAGGAATGACAATCACAAAGCTTGA
  GGACAGTGAAAATCATAGAAATGCATTTGAAATATCAGGGAGCATGATTGAGCGGATATTAGTGTCGTGC
  AACAACCAGCAGGATCTGCAGGAATGGGTGGAGCACCTACAGAAGCAAACGAAGGTCACGTCTGTGGGAA
  ACCCCACCATAAAGCCTCATTCAGTGCCATCTCATACCCTCCCCTCCCACCCGGTCACTCCGTCCAGCAA
  GCACGCAGACAGCAAGCCCGCGCCGCTGACGCCCGCCTACCACACGCTGCCCCACCCCTCCCACCACGGC
  ACCCCGCACACCACCATCAACTGGGGACCCCTGGAGCCTCCGAAAACACCCAAGCCCTGGAGCCTGAGCT
  GCCTGCGGCCCGCGCCTCCCCTCCGGCCCTCAGCTGCTCTCTGCTACAAGGAGGATCTTAGTAAGAGCCC
  TAAGACCATGAAAAAGCTGCTGCCCAAGCGCAAACCTGAACGGAAGCCTTCAGATGAGGAGTTCGCGTCC
  CGGAAAAGCACAGCTGCTTTGGAAGAAGATGCTCAGATTCTGAAAGTCATTGAAGCTTACTGCACCAGCG
  CCAAAACAAGGCAAACACTCAATTCAACATGGCAAGGCACTGACCTGATGCATAATCACGTCTTGGCTGA
  TGATGACCAACCAAGCCTAGACTCCCTGGGGCGTCGCAGTAGCCTTTCTCGTTTGGAGCCTTCAGACCTC
  TCGGAAGACTCTGACTATGACAGTATATGGACAGCCCATAGTTACAGAATGGGTTCTACATCTCGTAAGA
  GCTGTTGCTCATATATCTCTCACCAGAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226174 representing NM_001113511
  Red=Cloning site Green=Tags(s)
  
  MNSAEQTVTWLITLGVLESPKKTISDPEGFLQASLKDGVVLCRLLERLLPGTIEKVYPEPRSESECLSNI
  REFLRGCGASLRLELLFPPSQPPQHLVTTILLSASTFDANDLYQGQNFNKVLSSLVTLNKVTADIGLGSD
  SVCARPSSHRIKSFDSLGSQSLHTRTSKLFQGQYRSLDMTDNSNNQLVVRAKFNFQQTNEDELSFSKGDV
  IHVTRVEEGGWWEGTLNGRTGWFPSNYVREVKASEKPVSPKSGTLKSPPKGFDTTAINKSYYNVVLQNIL
  ETENEYSKELQTVLSTYLRPLQTSEKLSSANISYLMGNLEEICSFQQMLVQSLEECTKLPEAQQRVGGCF
  LNLMPQMKTLYLTYCANHPSAVNVLTEHSEELGEFMETKGASSPGILVLTTGLSKPFMRLDKYPTLLKEL
  ERHMEDYHTDRQDIQKSMAAFKNLSAQCQEVRKRKELELQILTEAIRNWEGDDIKTLGNVTYMSQVLIQC
  AGSEEKNERYLLLFPNVLLMLSASPRMSGFIYQGKLPTTGMTITKLEDSENHRNAFEISGSMIERILVSC
  NNQQDLQEWVEHLQKQTKVTSVGNPTIKPHSVPSHTLPSHPVTPSSKHADSKPAPLTPAYHTLPHPSHHG
  TPHTTINWGPLEPPKTPKPWSLSCLRPAPPLRPSAALCYKEDLSKSPKTMKKLLPKRKPERKPSDEEFAS
  RKSTAALEEDAQILKVIEAYCTSAKTRQTLNSTWQGTDLMHNHVLADDDQPSLDSLGRRSSLSRLEPSDL
  SEDSDYDSIWTAHSYRMGSTSRKSCCSYISHQN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001113511
• ORF Size: 2409 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001113511.1, NP_001106983.1
• RefSeq Size: 5525
• RefSeq ORF: 2412
• Locus ID: 8874
• Protein Pathways: Regulation of actin cytoskeleton
• Gene Summary: This gene encodes a protein that belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffles and to focal adhesions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Mar 2016]