PDE4A (NM_001111307) Human Tagged ORF Clone

CAT#: RG226235

PDE4A (GFP-tagged) - Human phosphodiesterase 4A, cAMP-specific (PDE4A), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PDE4A
• Synonyms: DPDE2; PDE4; PDE46
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226235 representing NM_001111307
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCCCCGACCGTCCCCTCGGAAAGGAGCCTGTCTCTGTCACTGCCCGGGCCCCGGGAGGGCCAGG
  CCACCCTGAAGCCTCCCCCGCAGCACCTGTGGCGGCAGCCTCGGACCCCCATCCGTATCCAGCAGCGCGG
  CTACTCCGACAGCGCGGAGCGCGCCGAGCGGGAGCGGCAGCCGCACCGGCCCATAGAGCGCGCCGATGCC
  ATGGACACCAGCGACCGGCCCGGCCTGCGCACGACCCGCATGTCCTGGCCCTCGTCCTTCCATGGCACTG
  GCACCGGCAGCGGCGGCGCGGGCGGAGGCAGCAGCAGGCGCTTCGAGGCAGAGAATGGGCCGACACCATC
  TCCTGGCCGCAGCCCCCTGGACTCGCAGGCGAGCCCAGGACTCGTGCTGCACGCCGGGGCGGCCACCAGC
  CAGCGCCGGGAGTCCTTCCTGTACCGCTCAGACAGCGACTATGACATGTCACCCAAGACCATGTCCCGGA
  ACTCATCGGTCACCAGCGAGGCGCACGCTGAAGACCTCATCGTAACACCATTTGCTCAGGTGCTGGCCAG
  CCTCCGGAGCGTCCGTAGCAACTTCTCACTCCTGACCAATGTGCCCGTTCCCAGTAACAAGCGGTCCCCG
  CTGGGCGGCCCCACCCCTGTCTGCAAGGCCACGCTGTCAGAAGAAACGTGTCAGCAGTTGGCCCGGGAGA
  CTCTGGAGGAGCTGGACTGGTGTCTGGAGCAGCTGGAGACCATGCAGACCTATCGCTCTGTCAGCGAGAT
  GGCCTCGCACAAGTTCAAAAGGATGTTGAACCGTGAGCTCACACACCTGTCAGAAATGAGCAGGTCCGGA
  AACCAGGTCTCAGAGTACATTTCCACAACATTCCTGGACAAACAGAATGAAGTGGAGATCCCATCACCCA
  CGATGAAGGAACGAGAAAAACAGCAAGCGCCGCGACCAAGACCCTCCCAGCCGCCCCCGCCCCCTGTACC
  ACACTTACAGCCCATGTCCCAAATCACAGGGTTGAAAAAGTTGATGCATAGTAACAGCCTGAACAACTCT
  AACATTCCCCGATTTGGGGTGAAGACCGATCAAGAAGAGCTCCTGGCCCAAGAACTGGAGAACCTGAACA
  AGTGGGGCCTGAACATCTTTTGCGTGTCGGATTACGCTGGAGGCCGCTCACTCACCTGCATCATGTACAT
  GATATTCCAGGAGCGGGACCTGCTGAAGAAATTCCGCATCCCTGTGGACACGATGGTGACATACATGCTG
  ACGCTGGAGGATCACTACCACGCTGACGTGGCCTACCATAACAGCCTGCACGCAGCTGACGTGCTGCAGT
  CCACCCACGTACTGCTGGCCACGCCTGCACTAGATGCAGTGTTCACGGACCTGGAGATTCTCGCCGCCCT
  CTTCGCGGCTGCCATCCACGATGTGGATCACCCTGGGGTCTCCAACCAGTTCCTCATCAACACCAATTCG
  GAGCTGGCGCTCATGTACAACGATGAGTCGGTGCTCGAGAATCACCACCTGGCCGTGGGCTTCAAGCTGC
  TGCAGGAGGACAACTGCGACATCTTCCAGAACCTCAGCAAGCGCCAGCGGCAGAGCCTACGCAAGATGGT
  CATCGACATGGTGCTGGCCACGGACATGTCCAAGCACATGACCCTCCTGGCTGACCTGAAGACCATGGTG
  GAGACCAAGAAAGTGACCAGCTCAGGGGTCCTCCTGCTAGATAACTACTCCGACCGCATCCAGGTCCTCC
  GGAACATGGTGCACTGTGCCGACCTCAGCAACCCCACCAAGCCGCTGGAGCTGTACCGCCAGTGGACAGA
  CCGCATCATGGCCGAGTTCTTCCAGCAGGGTGACCGAGAGCGCGAGCGTGGCATGGAAATCAGCCCCATG
  TGTGACAAGCACACTGCCTCCGTGGAGAAGTCTCAGGTGGGTTTTATTGACTACATTGTGCACCCATTGT
  GGGAGACCTGGGCGGACCTTGTCCACCCAGATGCCCAGGAGATCTTGGACACTTTGGAGGACAACCGGGA
  CTGGTACTACAGCGCCATCCGGCAGAGCCCATCTCCGCCACCCGAGGAGGAGTCAAGGGGGCCAGGCCAC
  CCACCCCTGCCTGACAAGTTCCAGTTTGAGCTGACGCTGGAGGAGGAAGAGGAGGAAGAAATATCAATGG
  CCCAGATACCGTGCACAGCCCAAGAGGCATTGACTGCGCAGGGATTGTCAGGAGTCGAGGAAGCTCTGGA
  TGCAACCATAGCCTGGGAGGCATCCCCGGCCCAGGAGTCGTTGGAAGTTATGGCACAGGAAGCATCCCTG
  GAGGCCGAGCTGGAGGCAGTGTATTTGACACAGCAGGCACAGTCCACAGGCAGTGCACCTGTGGCTCCGG
  ATGAGTTCTCGTCCCGGGAGGAATTCGTGGTTGCTGTAAGCCACAGCAGCCCCTCTGCCCTGGCTCTTCA
  AAGCCCCCTTCTCCCTGCTTGGAGGACCCTGTCTGTTTCAGAGCATGCCCCGGGCCTCCCGGGCCTCCCC
  TCCACGGCGGCCGAGGTGGAGGCCCAACGAGAGCACCAGGCTGCCAAGAGGGCTTGCAGTGCCTGCGCAG
  GGACATTTGGGGAGGACACATCCGCACTCCCAGCTCCTGGTGGCGGGGGGTCAGGTGGAGACCCTACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226235 representing NM_001111307
  Red=Cloning site Green=Tags(s)
  
  MEPPTVPSERSLSLSLPGPREGQATLKPPPQHLWRQPRTPIRIQQRGYSDSAERAERERQPHRPIERADA
  MDTSDRPGLRTTRMSWPSSFHGTGTGSGGAGGGSSRRFEAENGPTPSPGRSPLDSQASPGLVLHAGAATS
  QRRESFLYRSDSDYDMSPKTMSRNSSVTSEAHAEDLIVTPFAQVLASLRSVRSNFSLLTNVPVPSNKRSP
  LGGPTPVCKATLSEETCQQLARETLEELDWCLEQLETMQTYRSVSEMASHKFKRMLNRELTHLSEMSRSG
  NQVSEYISTTFLDKQNEVEIPSPTMKEREKQQAPRPRPSQPPPPPVPHLQPMSQITGLKKLMHSNSLNNS
  NIPRFGVKTDQEELLAQELENLNKWGLNIFCVSDYAGGRSLTCIMYMIFQERDLLKKFRIPVDTMVTYML
  TLEDHYHADVAYHNSLHAADVLQSTHVLLATPALDAVFTDLEILAALFAAAIHDVDHPGVSNQFLINTNS
  ELALMYNDESVLENHHLAVGFKLLQEDNCDIFQNLSKRQRQSLRKMVIDMVLATDMSKHMTLLADLKTMV
  ETKKVTSSGVLLLDNYSDRIQVLRNMVHCADLSNPTKPLELYRQWTDRIMAEFFQQGDRERERGMEISPM
  CDKHTASVEKSQVGFIDYIVHPLWETWADLVHPDAQEILDTLEDNRDWYYSAIRQSPSPPPEEESRGPGH
  PPLPDKFQFELTLEEEEEEEISMAQIPCTAQEALTAQGLSGVEEALDATIAWEASPAQESLEVMAQEASL
  EAELEAVYLTQQAQSTGSAPVAPDEFSSREEFVVAVSHSSPSALALQSPLLPAWRTLSVSEHAPGLPGLP
  STAAEVEAQREHQAAKRACSACAGTFGEDTSALPAPGGGGSGGDPT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001111307
• ORF Size: 2658 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001111307.1, NP_001104777.1
• RefSeq Size: 4794 bp
• RefSeq ORF: 2661 bp
• Locus ID: 5141
• Cytogenetics: 19p13.2
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: 'The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]'