SMARCA1 (NM_139035) Human Tagged ORF Clone

CAT#: RG226308

SMARCA1 (GFP-tagged) - Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1 (SMARCA1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SMARCA1
• Synonyms: ISWI; NURF140; SNF2L; SNF2L1; SNF2LB; SNF2LT; SWI; SWI2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226308 representing NM_139035
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAGGACACTGCCGCAGTGGCAGCCACCGTGGCAGCCGCGGATGCGACCGCCACTATCGTGGTCA
  TAGAGGACGAGCAGCCCGGGCCGTCCACCTCTCAGGAGGAGGGAGCGGCCGCCGCGGCCACCGAAGCCAC
  CGCGGCCACGGAGAAGGGCGAGAAGAAGAAGGAGAAAAACGTTTCTTCATTTCAACTCAAACTTGCTGCT
  AAAGCGCCTAAATCTGAAAAGGAAATGGACCCAGAATATGAAGAGAAAATGAAAGCCGACCGAGCAAAGA
  GATTTGAATTTTTACTGAAGCAGACAGAACTTTTTGCACATTTCATTCAGCCTTCAGCACAGAAATCTCC
  AACATCTCCACTGAACATGAAATTGGGACGTCCCCGAATAAAGAAAGATGAAAAGCAGAGCTTAATTTCT
  GCTGGAGACTACCGCCATAGGCGCACAGAGCAAGAAGAAGATGAAGAGCTACTGTCTGAGAGTCGGAAAA
  CATCTAATGTGTGTATTAGATTTGAGGTGTCACCTTCATATGTGAAAGGGGGGCCACTGAGAGATTATCA
  GATTCGAGGACTGAATTGGTTGATCTCTTTATATGAAAATGGAGTCAATGGCATTTTGGCTGATGAAATG
  GGCCTTGGGAAAACTTTACAAACAATTGCTTTGCTTGGTTACCTGAAACACTACCGAAATATTCCTGGAC
  CTCACATGGTTTTAGTTCCAAAGTCTACTTTACACAACTGGATGAATGAATTTAAACGATGGGTCCCATC
  TCTCCGTGTCATTTGTTTTGTCGGAGACAAGGATGCCAGAGCTGCTTTTATTCGTGATGAAATGATGCCA
  GGAGAGTGGGATGTTTGCGTTACTTCTTATGAGATGGTAATTAAAGAAAAATCTGTATTCAAAAAGTTTC
  ACTGGCGATACCTGGTCATTGATGAAGCTCACAGAATAAAGAATGAAAAATCTAAGCTTTCAGAGATTGT
  TCGTGAGTTCAAGTCGACTAACCGCTTGCTCCTAACTGGAACACCTTTGCAGAATAACCTGCATGAACTG
  TGGGCCTTACTCAACTTTTTATTGCCTGATGTCTTTAATTCTGCAGATGACTTTGATTCTTGGTTTGACA
  CTAAAAATTGTCTTGGTGATCAAAAACTCGTGGAAAGACTTCATGCAGTTTTAAAACCATTTTTGTTACG
  CCGTATAAAAACTGATGTAGAGAAGAGTCTGCCACCTAAAAAGGAAATAAAGATTTACTTGGGGCTGAGT
  AAGATGCAACGAGAATGGTATACAAAAATCCTGATGAAAGATATTGATGTTTTAAACTCTTCTGGCAAGA
  TGGACAAGATGCGACTCTTAAACATTCTGATGCAGCTTCGAAAGTGTTGTAATCATCCATATCTGTTTGA
  TGGTGCTGAACCTGGTCCACCTTATACCACTGATGAGCATATTGTCAGCAACAGTGGTAAAATGGTAGTT
  CTGGATAAACTATTGGCCAAACTCAAAGAACAGGGTTCAAGGGTTCTCATTTTCAGCCAGATGACTCGCT
  TGCTGGATATTTTGGAAGATTATTGCATGTGGCGTGGTTATGAGTATTGTCGACTGGATGGACAAACCCC
  GCATGAAGAAAGAGAGGAAGCAATAGAGGCTTTTAATGCTCCTAATAGTAGCAAATTCATCTTTATGCTA
  AGTACCAGGGCTGGAGGTCTCGGAATTAACCTGGCAAGTGCTGATGTGGTTATACTATATGATTCAGACT
  GGAACCCACAGGTTGATCTACAAGCTATGGATCGAGCACATCGTATTGGTCAGAAGAAACCAGTACGTGT
  ATTCCGTCTCATCACTGACAACACTGTTGAAGAGAGGATTGTAGAAAGAGCTGAGATAAAACTGAGACTC
  GATTCAATTGTTATACAACAAGGAAGACTCATTGACCAACAGTCTAACAAGCTGGCAAAAGAGGAAATGT
  TACAAATGATACGGCATGGAGCCACCCATGTTTTTGCTTCTAAAGAGAGTGAGTTGACAGATGAAGACAT
  TACAACTATTCTGGAAAGAGGGGAAAAGAAGACTGCAGAGATGAATGAACGCCTGCAAAAAATGGGAGAG
  TCTTCTCTAAGAAATTTTAGAATGGACATTGAACAAAGTTTATACAAATTTGAGGGAGAAGATTATAGAG
  AAAAACAGAAGCTTGGCATGGTGGAATGGATTGAACCTCCTAAACGAGAACGCAAAGCAAACTACGCAGT
  GGATGCCTACTTTAGAGAGGCTTTGCGTGTCAGCGAGCCAAAGATTCCAAAGGCTCCACGGCCTCCAAAA
  CAGCCAAATGTTCAGGATTTTCAATTTTTCCCACCACGCTTATTTGAGCTCCTGGAAAAGGAAATTCTTT
  ATTATCGGAAGACAATAGGCTATAAGGTTCCAAGGAATCCTGATATCCCAAATCCAGCTCTGGCTCAAAG
  AGAAGAGCAAAAAAAGATTGATGGAGCTGAACCTCTTACACCAGAAGAGACTGAAGAAAAGGAAAAACTT
  CTCACACAAGGTTTCACAAACTGGACTAAACGAGATTTTAACCAGTTTATTAAAGCTAATGAGAAATATG
  GAAGAGATGACATTGATAACATAGCTCGAGAGGTAGAGGGCAAATCCCCTGAGGAGGTCATGGAGTATTC
  AGCTGTATTTTGGGAACGTTGCAATGAATTACAGGACATTGAGAAAATTATGGCTCAAATTGAACGTGGA
  GAAGCAAGAATTCAACGAAGGATCAGTATCAAGAAAGCCCTGGATGCCAAAATTGCAAGATACAAGGCTC
  CATTTCATCAGTTGCGCATTCAGTATGGAACCAGCAAAGGAAAGAACTATACTGAGGAAGAAGATAGATT
  CTTGATTTGTATGTTACACAAAATGGGCTTTGATAGAGAAAATGTATATGAAGAATTAAGACAGTGTGTA
  CGAAATGCTCCCCAGTTTAGATTTGACTGGTTTATCAAGTCTAGGACTGCCATGGAATTCCAGAGACGCT
  GTAACACTCTGATTTCATTGATTGAGAAAGAAAATATGGAAATTGAGGAAAGAGAGAGAGCAGAAAAGAA
  GAAACGGGCAACTAAAACTCCAATGGTAAAATTTTCAGCATTTTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226308 representing NM_139035
  Red=Cloning site Green=Tags(s)
  
  MEQDTAAVAATVAAADATATIVVIEDEQPGPSTSQEEGAAAAATEATAATEKGEKKKEKNVSSFQLKLAA
  KAPKSEKEMDPEYEEKMKADRAKRFEFLLKQTELFAHFIQPSAQKSPTSPLNMKLGRPRIKKDEKQSLIS
  AGDYRHRRTEQEEDEELLSESRKTSNVCIRFEVSPSYVKGGPLRDYQIRGLNWLISLYENGVNGILADEM
  GLGKTLQTIALLGYLKHYRNIPGPHMVLVPKSTLHNWMNEFKRWVPSLRVICFVGDKDARAAFIRDEMMP
  GEWDVCVTSYEMVIKEKSVFKKFHWRYLVIDEAHRIKNEKSKLSEIVREFKSTNRLLLTGTPLQNNLHEL
  WALLNFLLPDVFNSADDFDSWFDTKNCLGDQKLVERLHAVLKPFLLRRIKTDVEKSLPPKKEIKIYLGLS
  KMQREWYTKILMKDIDVLNSSGKMDKMRLLNILMQLRKCCNHPYLFDGAEPGPPYTTDEHIVSNSGKMVV
  LDKLLAKLKEQGSRVLIFSQMTRLLDILEDYCMWRGYEYCRLDGQTPHEEREEAIEAFNAPNSSKFIFML
  STRAGGLGINLASADVVILYDSDWNPQVDLQAMDRAHRIGQKKPVRVFRLITDNTVEERIVERAEIKLRL
  DSIVIQQGRLIDQQSNKLAKEEMLQMIRHGATHVFASKESELTDEDITTILERGEKKTAEMNERLQKMGE
  SSLRNFRMDIEQSLYKFEGEDYREKQKLGMVEWIEPPKRERKANYAVDAYFREALRVSEPKIPKAPRPPK
  QPNVQDFQFFPPRLFELLEKEILYYRKTIGYKVPRNPDIPNPALAQREEQKKIDGAEPLTPEETEEKEKL
  LTQGFTNWTKRDFNQFIKANEKYGRDDIDNIAREVEGKSPEEVMEYSAVFWERCNELQDIEKIMAQIERG
  EARIQRRISIKKALDAKIARYKAPFHQLRIQYGTSKGKNYTEEEDRFLICMLHKMGFDRENVYEELRQCV
  RNAPQFRFDWFIKSRTAMEFQRRCNTLISLIEKENMEIEERERAEKKKRATKTPMVKFSAFS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139035
• ORF Size: 3126 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139035.2, NP_620604.2
• RefSeq Size: 4066 bp
• RefSeq ORF: 3128 bp
• Locus ID: 6594
• Cytogenetics: Xq25-q26.1
• Domains: SNF2_N, myb_DNA-binding, DEAD, helicase_C
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a member of the SWI/SNF family of proteins. The encoded protein is an ATPase which is expressed in diverse tissues and contributes to the chromatin remodeling complex that is involved in transcription. The protein may also play a role in DNA damage, growth inhibition and apoptosis of cancer cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]'