NMDAR2A (GRIN2A) (NM_001134407) Human Tagged ORF Clone

CAT#: RG226397

GRIN2A (GFP-tagged) - Human glutamate receptor, ionotropic, N-methyl D-aspartate 2A (GRIN2A), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GRIN2A
• Synonyms: EPND; FESD; GluN2A; LKS; NMDAR2A; NR2A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226397 representing NM_001134407
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAGAGTGGGCTATTGGACCCTGCTGGTGCTGCCGGCCCTTCTGGTCTGGCGCGGTCCGGCGCCGA
  GCGCGGCGGCGGAGAAGGGTCCCCCCGCGCTAAATATTGCGGTGATGCTGGGTCACAGCCACGACGTGAC
  AGAGCGCGAACTTCGAACACTGTGGGGCCCCGAGCAGGCGGCGGGGCTGCCCCTGGACGTGAACGTGGTA
  GCTCTGCTGATGAACCGCACCGACCCCAAGAGCCTCATCACGCACGTGTGCGACCTCATGTCCGGGGCAC
  GCATCCACGGCCTCGTGTTTGGGGACGACACGGACCAGGAGGCCGTAGCCCAGATGCTGGATTTTATCTC
  CTCCCACACCTTCGTCCCCATCTTGGGCATTCATGGGGGCGCATCTATGATCATGGCTGACAAGGATCCG
  ACGTCTACCTTCTTCCAGTTTGGAGCGTCCATCCAGCAGCAAGCCACGGTCATGCTGAAGATCATGCAGG
  ATTATGACTGGCATGTCTTCTCCCTGGTGACCACTATCTTCCCTGGCTACAGGGAATTCATCAGCTTCGT
  CAAGACCACAGTGGACAACAGCTTTGTGGGCTGGGACATGCAGAATGTGATCACACTGGACACTTCCTTT
  GAGGATGCAAAGACACAAGTCCAGCTGAAGAAGATCCACTCTTCTGTCATCTTGCTCTACTGTTCCAAAG
  ACGAGGCTGTTCTCATTCTGAGTGAGGCCCGCTCCCTTGGCCTCACCGGGTATGATTTCTTCTGGATTGT
  CCCCAGCTTGGTCTCTGGGAACACGGAGCTCATCCCAAAAGAGTTTCCATCGGGACTCATTTCTGTCTCC
  TACGATGACTGGGACTACAGCCTGGAGGCGAGAGTGAGGGACGGCATTGGCATCCTAACCACCGCTGCAT
  CTTCTATGCTGGAGAAGTTCTCCTACATCCCCGAGGCCAAGGCCAGCTGCTACGGGCAGATGGAGAGGCC
  AGAGGTCCCGATGCACACCTTGCACCCATTTATGGTCAATGTTACATGGGATGGCAAAGACTTATCCTTC
  ACTGAGGAAGGCTACCAGGTGCACCCCAGGCTGGTGGTGATTGTGCTGAACAAAGACCGGGAATGGGAAA
  AGGTGGGCAAGTGGGAGAACCATACGCTGAGCCTGAGGCACGCCGTGTGGCCCAGGTACAAGTCCTTCTC
  CGACTGTGAGCCGGATGACAACCATCTCAGCATCGTCACCCTGGAGGAGGCCCCATTCGTCATCGTGGAA
  GACATAGACCCCCTGACCGAGACGTGTGTGAGGAACACCGTGCCATGTCGGAAGTTCGTCAAAATCAACA
  ATTCAACCAATGAGGGGATGAATGTGAAGAAATGCTGCAAGGGGTTCTGCATTGATATTCTGAAGAAGCT
  TTCCAGAACTGTGAAGTTTACTTACGACCTCTATCTGGTGACCAATGGGAAGCATGGCAAGAAAGTTAAC
  AATGTGTGGAATGGAATGATCGGTGAAGTGGTCTATCAACGGGCAGTCATGGCAGTTGGCTCGCTCACCA
  TCAATGAGGAACGTTCTGAAGTGGTGGACTTCTCTGTGCCCTTTGTGGAAACGGGAATCAGTGTCATGGT
  TTCAAGAAGTAATGGCACCGTCTCACCTTCTGCTTTTCTAGAACCATTCAGCGCCTCTGTCTGGGTGATG
  ATGTTTGTGATGCTGCTCATTGTTTCTGCCATAGCTGTTTTTGTCTTTGAATACTTCAGCCCTGTTGGAT
  ACAACAGAAACTTAGCCAAAGGGAAAGCACCCCATGGGCCTTCTTTTACAATTGGAAAAGCTATATGGCT
  TCTTTGGGGCCTGGTGTTCAATAACTCCGTGCCTGTCCAGAATCCTAAAGGGACCACCAGCAAGATCATG
  GTATCTGTATGGGCCTTCTTCGCTGTCATATTCCTGGCTAGCTACACAGCCAATCTGGCTGCCTTCATGA
  TCCAAGAGGAATTTGTGGACCAAGTGACCGGCCTCAGTGACAAAAAGTTTCAGAGACCTCATGACTATTC
  CCCACCTTTTCGATTTGGGACAGTGCCTAATGGAAGCACGGAGAGAAACATTCGGAATAACTATCCCTAC
  ATGCATCAGTACATGACCAAATTTAATCAGAAAGGAGTAGAGGACGCCTTGGTCAGCCTGAAAACGGGGA
  AGCTGGACGCTTTCATCTACGATGCCGCAGTCTTGAATTACAAGGCTGGGAGGGATGAAGGCTGCAAGCT
  GGTGACCATCGGGAGTGGGTACATCTTTGCCACCACCGGTTATGGAATTGCCCTTCAGAAAGGCTCTCCT
  TGGAAGAGGCAGATCGACCTGGCCTTGCTTCAGTTTGTGGGTGATGGTGAGATGGAGGAGCTGGAGACCC
  TGTGGCTCACTGGGATCTGCCACAACGAGAAGAACGAGGTGATGAGCAGCCAGCTGGACATTGACAACAT
  GGCGGGCGTATTCTACATGCTGGCTGCCGCCATGGCCCTTAGCCTCATCACCTTCATCTGGGAGCACCTC
  TTCTACTGGAAGCTGCGCTTCTGTTTCACGGGCGTGTGCTCCGACCGGCCTGGGTTGCTCTTCTCCATCA
  GCAGGGGCATCTACAGCTGCATTCATGGAGTGCACATTGAAGAAAAGAAGAAGTCTCCAGACTTCAATCT
  GACGGGATCCCAGAGCAACATGTTAAAACTCCTCCGGTCAGCCAAAAACATTTCCAGCATGTCCAACATG
  AACTCCTCAAGAATGGACTCACCCAAAAGAGCTGCTGACTTCATCCAAAGAGGTTCCCTCATCATGGACA
  TGGTTTCAGATAAGGGGAATTTGATGTACTCAGACAACAGGTCCTTTCAGGGGAAAGAGAGCATTTTTGG
  AGACAACATGAACGAACTCCAAACATTTGTGGCCAACCGGCAGAAGGATAACCTCAATAACTATGTATTC
  CAGGGACAACATCCTCTTACTCTCAATGAGTCCAACCCTAACACGGTGGAGGTGGCCGTGAGCACAGAAT
  CCAAAGCGAACTCTAGACCCCGGCAGCTGTGGAAGAAATCCGTGGATTCCATACGCCAGGATTCACTATC
  CCAGAATCCAGTCTCCCAGAGGGATGAGGCAACAGCAGAGAATAGGACCCACTCCCTAAAGAGCCCTAGG
  TATCTTCCAGAAGAGATGGCCCACTCTGACATTTCAGAAACGTCAAATCGGGCCACGTGCCACAGGGAAC
  CTGACAACAGTAAGAACCACAAAACCAAGGACAACTTTAAAAGGTCAGTGGCCTCCAAATACCCCAAGGA
  CTGTAGTGAGGTCGAGCGCACCTACCTGAAAACCAAATCAAGCTCCCCTAGAGACAAGATCTACACTATA
  GATGGTGAGAAGGAGCCTGGTTTCCACTTAGATCCACCCCAGTTTGTTGAAAATGTGACCCTGCCCGAGA
  ACGTGGACTTCCCGGACCCCTACCAGGATCCCAGTGAAAACTTCCGCAAGGGGGACTCCACGCTGCCAAT
  GAACCGGAACCCCTTGCATAATGAAGAGGGGCTTTCCAACAACGACCAGTATAAACTCTACTCCAAGCAC
  TTCACCTTGAAAGACAAGGGTTCCCCGCACAGTGAGACCAGCGAGCGATACCGGCAGAACTCCACGCACT
  GCAGAAGCTGCCTTTCCAACATGCCCACCTATTCAGGCCACTTCACCATGAGGTCCCCCTTCAAGTGCGA
  TGCCTGCCTGCGGATGGGGAACCTCTATGACATCGATGAAGACCAGATGCTTCAGGAGACAGGTAACCCA
  GCCACCGGGGAGCAGGTCTACCAGCAGGACTGGGCACAGAACAATGCCCTTCAATTACAAAAGAACAAGC
  TAAGGATTAGCCGTCAGCATTCCTACGATAACATTGTCGACAAACCTAGGGAGCTAGACCTTAGCAGGCC
  CTCCCGGAGCATAAGCCTCAAGGACAGGGAACGGCTTCTGGAGGGAAATTTTTACGGCAGCCTGTTTAGT
  GTCCCCTCAAGCAAACTCTCGGGGAAAAAAAGCTCCCTTTTCCCCCAAGGTCTGGAGGACAGCAAGAGGA
  GCAAGTCTCTCTTGCCAGACCACACCTCCGATAACCCTTTCCTCCACTCCCACAGGGATGACCAACGCTT
  GGTTATTGGGAGATGCCCCTCGGACCCTTACAAACACTCGTTGCCATCCCAGGCGGTGAATGACAGCTAT
  CTTCGGTCGTCCTTGAGGTCAACGGCATCGTACTGTTCCAGGGACAGTCGGGGCCACAATGATGTGTATA
  TTTCGGAGCATGTTATGCCTTATGCTGCAAATAAGAATAATATGTACTCTACCCCCAGGGTTTTAAATTC
  CTGCAGCAATAGACGCGTGTACAAGAAAATGCCTAGTATCGAATCTGATGTT
  
  ACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226397 representing NM_001134407
  Red=Cloning site Green=Tags(s)
  
  MGRVGYWTLLVLPALLVWRGPAPSAAAEKGPPALNIAVMLGHSHDVTERELRTLWGPEQAAGLPLDVNVV
  ALLMNRTDPKSLITHVCDLMSGARIHGLVFGDDTDQEAVAQMLDFISSHTFVPILGIHGGASMIMADKDP
  TSTFFQFGASIQQQATVMLKIMQDYDWHVFSLVTTIFPGYREFISFVKTTVDNSFVGWDMQNVITLDTSF
  EDAKTQVQLKKIHSSVILLYCSKDEAVLILSEARSLGLTGYDFFWIVPSLVSGNTELIPKEFPSGLISVS
  YDDWDYSLEARVRDGIGILTTAASSMLEKFSYIPEAKASCYGQMERPEVPMHTLHPFMVNVTWDGKDLSF
  TEEGYQVHPRLVVIVLNKDREWEKVGKWENHTLSLRHAVWPRYKSFSDCEPDDNHLSIVTLEEAPFVIVE
  DIDPLTETCVRNTVPCRKFVKINNSTNEGMNVKKCCKGFCIDILKKLSRTVKFTYDLYLVTNGKHGKKVN
  NVWNGMIGEVVYQRAVMAVGSLTINEERSEVVDFSVPFVETGISVMVSRSNGTVSPSAFLEPFSASVWVM
  MFVMLLIVSAIAVFVFEYFSPVGYNRNLAKGKAPHGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIM
  VSVWAFFAVIFLASYTANLAAFMIQEEFVDQVTGLSDKKFQRPHDYSPPFRFGTVPNGSTERNIRNNYPY
  MHQYMTKFNQKGVEDALVSLKTGKLDAFIYDAAVLNYKAGRDEGCKLVTIGSGYIFATTGYGIALQKGSP
  WKRQIDLALLQFVGDGEMEELETLWLTGICHNEKNEVMSSQLDIDNMAGVFYMLAAAMALSLITFIWEHL
  FYWKLRFCFTGVCSDRPGLLFSISRGIYSCIHGVHIEEKKKSPDFNLTGSQSNMLKLLRSAKNISSMSNM
  NSSRMDSPKRAADFIQRGSLIMDMVSDKGNLMYSDNRSFQGKESIFGDNMNELQTFVANRQKDNLNNYVF
  QGQHPLTLNESNPNTVEVAVSTESKANSRPRQLWKKSVDSIRQDSLSQNPVSQRDEATAENRTHSLKSPR
  YLPEEMAHSDISETSNRATCHREPDNSKNHKTKDNFKRSVASKYPKDCSEVERTYLKTKSSSPRDKIYTI
  DGEKEPGFHLDPPQFVENVTLPENVDFPDPYQDPSENFRKGDSTLPMNRNPLHNEEGLSNNDQYKLYSKH
  FTLKDKGSPHSETSERYRQNSTHCRSCLSNMPTYSGHFTMRSPFKCDACLRMGNLYDIDEDQMLQETGNP
  ATGEQVYQQDWAQNNALQLQKNKLRISRQHSYDNIVDKPRELDLSRPSRSISLKDRERLLEGNFYGSLFS
  VPSSKLSGKKSSLFPQGLEDSKRSKSLLPDHTSDNPFLHSHRDDQRLVIGRCPSDPYKHSLPSQAVNDSY
  LRSSLRSTASYCSRDSRGHNDVYISEHVMPYAANKNNMYSTPRVLNSCSNRRVYKKMPSIESDV
  
  TRPLE - GFP Tag - V
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001134407
• ORF Size: 4392 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001134407.1, NP_001127879.1
• RefSeq Size: 14706 bp
• RefSeq ORF: 4395 bp
• Locus ID: 2903
• Cytogenetics: 16p13.2
• Protein Families: Druggable Genome, Ion Channels: Glutamate Receptors, Ion Channels: Sodium, Transmembrane
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Calcium signaling pathway, Long-term potentiation, Neuroactive ligand-receptor interaction, Systemic lupus erythematosus
• Gene Summary: 'This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without cognitive disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]'