ZFYVE16 (NM_001105251) Human Tagged ORF Clone

CAT#: RG226406

ZFYVE16 (GFP-tagged) - Human zinc finger, FYVE domain containing 16 (ZFYVE16), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZFYVE16
• Synonyms: PPP1R69
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226406 representing NM_001105251
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGTTATTTTAAAGCAGCTGTCAGTGACTTGGACAAACTCCTTGATGATTTTGAACAGAACCCAG
  ATGAACAAGATTATCTCCAAGATGTACAAAATGCATATGATTCTAACCACTGCTCAGTTTCTTCAGAGTT
  GGCTTCCTCACAGCGAACTTCATTGCTCCCAAAAGACCAAGAGTGCGTTAATAGTTGTGCCTCATCAGAA
  ACAAGCTATGGAACAAATGAGAGTTCCCTGAATGAAAAAACACTCAAGGGACTTACTTCTATACAAAATG
  AAAAAAATGTAACAGGACTTGATCTTCTTTCTTCTGTGGATGGTGGTACTTCAGATGAAATCCAGCCGTT
  ATATATGGGACGATGTAGTAAACCTATCTGTGATCTGATAAGTGACATGGGTAACTTAGTTCATGCAACC
  AATAGTGAAGAAGATATTAAAAAATTATTGCCAGATGATTTTAAGTCTAATGCAGATTCCTTGATTGGAT
  TGGATTTATCTTCAGTGTCAGATACTCCCTGTGTTTCTTCAACAGACCATGATAGTGATACTGTCAGAGA
  ACAACAGAATGATATCAGTTCTGAATTACAAAATAGAGAAATCGGAGGAATCAAAGAATTGGGTATAAAA
  GTAGATACAACACTTTCAGATTCCTATAATTACAGTGGAACAGAAAATTTAAAAGATAAAAAGATCTTTA
  ATCAGTTAGAATCAATTGTTGATTTTAACATGTCATCTGCTTTGACTCGACAAAGTTCCAAAATGTTTCA
  TGCCAAAGACAAGCTACAACACAAGAGCCAGCCATGTGGATTACTAAAAGATGTTGGCTTAGTAAAAGAG
  GAAGTAGATGTGGCAGTCATAACTGCCGCAGAATGTTTAAAAGAAGAGGGCAAGACAAGTGCTTTGACCT
  GCAGCCTTCCGAAAAATGAAGATTTATGCTTAAATGATTCAAATTCAAGAGATGAAAATTTCAAATTACC
  TGACTTTTCCTTTCAGGAAGATAAGACTGTTATAAAACAATCTGCACAAGAAGACTCAAAAAGTTTAGAC
  CTTAAGGATAATGATGTAATCCAAGATTCCTCTTCAGCTTTACATGTTTCCAGTAAAGATGTGCCGTCCT
  CATTGTCCTGTCTTCCTGCGTCTGGGTCTATGTGTGGATCATTAATTGAAAGTAAAGCACGGGGTGATTT
  TTTACCTCAGCATGAACATAAAGATAATATACAAGATGCAGTGACTATACATGAAGAAATACAGAACAGT
  GTTGTTCTAGGTGGGGAACCATTCAAAGAGAATGATCTTTTGAAACAGGAAAAATGTAAAAGCATACTCC
  TTCAGTCATTAATTGAAGGGATGGAAGACAGAAAGATAGATCCTGACCAGACAGTAATCAGAGCTGAGTC
  TTTGGATGGTGGTGACACCAGTTCTACAGTTGTAGAATCTCAAGAGGGGCTTTCTGGCACTCATGTCCCA
  GAGTCTTCTGATTGTTGTGAAGGTTTTATTAATACTTTTTCAAGCAATGATATGGATGGGCAAGACTTAG
  ATTACTTTAATATTGATGAAGGCGCAAAAAGTGGCCCACTAATTAGTGATGCTGAACTTGATGCCTTTCT
  GACAGAACAGTATCTTCAGACCACTAACATAAAGTCTTTTGAAGAAAATGTAAATGACTCTAAATCGCAA
  ATGAATCAGATAGATATGAAAGGCTTAGATGATGGAAACATCAATAATATATATTTCAATGCAGAAGCAG
  GAGCTATTGGGGAAAGTCATGGTATTAATATAATTTGTGAAACAGTTGATAAACAAAATACAATAGAAAA
  TGGCCTTTCTTTAGGAGAAAAAAGCACTATTCCAGTTCAACAAGGGTTACCTACCAGTAAGTCTGAGATT
  ACAAATCAATTATCAGTCTCTGATATTAACAGTCAATCTGTTGGAGGGGCCAGACCTAAGCAATTGTTTA
  GCCTTCCATCAAGAACAAGGAGTTCAAAGGACCTGAATAAGCCAGATGTTCCAGATACAATAGAAAGTGA
  ACCCAGCACAGCAGATACCGTTGTTCCAATCACTTGTGCTATAGATTCTACAGCTGATCCACAGGTTAGC
  TTCAACTCTAATTACATTGATATAGAAAGTAATTCTGAAGGTGGATCTAGTTTCGTAACTGCAAATGAAG
  ATTCTGTACCTGAAAACACTTGCAAAGAAGGCTTGGTTTTGGGCCAGAAACAGCCTACTTGGGTTCCTGA
  TTCAGAAGCTCCAAACTGTATGAACTGCCAAGTCAAATTTACTTTTACCAAACGGCGACACCATTGCCGA
  GCATGTGGGAAAGTATTTTGTGGTGTCTGTTGTAATAGGAAGTGTAAACTGCAATATCTAGAAAAGGAAG
  CAAGAGTATGTGTAGTCTGCTATGAAACTATTAGTAAAGCTCAGGCATTTGAAAGGATGATGAGTCCAAC
  TGGTTCTAATCTTAAGTCTAATCATTCTGATGAATGTACTACTGTCCAGCCTCCTCAGGAGAACCAAACA
  TCCAGTATACCTTCACCAGCAACTTTGCCAGTCTCAGCACTTAAACAACCAGGTGTTGAAGGACTATGTT
  CCAAAGAACAGAAGAGAGTATGGTTTGCAGATGGTATATTGCCCAATGGTGAAGTTGCAGATACAACAAA
  ATTATCATCTGGAAGTAAAAGATGTTCTGAAGACTTTAGTCCTCTCTCACCTGATGTGCCTATGACAGTA
  AACACAGTGGATCATTCCCATTCTACTACAGTGGAAAAGCCAAACAATGAGACAGGAGATATTACAAGAA
  ATGAGATAATTCAGAGTCCTATTTCTCAGGTTCCATCAGTGGAAAAATTGTCTATGAACACAGGAAATGA
  GGGGTTACCTACTTCTGGTTCATTTACACTAGATGATGATGTTTTTGCAGAAACTGAAGAACCATCTAGT
  CCTACTGGTGTCTTAGTTAACAGCAATTTACCTATTGCTAGTATTTCAGATTATAGGTTACTGTGTGATA
  TTAACAAGTATGTCTGCAATAAGATTAGTCTTCTACCTAATGATGAGGACAGTTTGCCCCCACTTCTGGT
  TGCATCTGGAGAAAAGGGATCAGTGCCTGTAGTAGAAGAACATCCATCTCATGAGCAGATCATTTTGCTT
  CTTGAAGGTGAAGGCTTTCATCCTGTTACATTTGTCCTAAATGCTAATCTACTCGTGAATGTCAAATTCA
  TATTTTATTCCTCAGACAAATATTGGTACTTTTCAACCAATGGATTGCATGGCTTGGGACAGGCAGAAAT
  TATTATTCTATTGTTATGTTTGCCAAATGAAGATACTATTCCTAAGGACATCTTCAGACTATTTATCACC
  ATATATAAGGATGCTCTAAAAGGAAAATACATAGAAAACTTGGACAATATTACCTTTACTGAGAGTTTTC
  TCAGTAGCAAGGATCACGGAGGATTCCTGTTTATTACACCTACTTTTCAGAAACTTGATGATCTCTCATT
  ACCAAGTAATCCTTTTCTTTGTGGAATTCTTATCCAGAAGCTTGAGATTCCCTGGGCAAAGGTTTTTCCT
  ATGCGTTTAATGTTGAGATTGGGTGCAGAATATAAAGCATATCCTGCTCCTCTAACAAGCATCAGAGGCC
  GAAAACCTCTTTTTGGAGAAATAGGACACACTATTATGAACTTACTTGTTGACCTTCGAAATTACCAGTA
  TACCTTGCATAATATAGATCAACTGTTGATTCATATGGAAATGGGAAAAAGCTGCATAAAAATACCACGG
  AAAAAGTACAGTGATGTAATGAAAGTACTAAATTCTTCCAATGAGCATGTCATTAGCATTGGAGCAAGTT
  TCAGTACAGAAGCAGATTCTCATCTAGTCTGTATACAGAATGATGGAATTTATGAAACACAGGCCAACAG
  TGCCACTGGCCATCCTAGAAAAGTGACAGGTGCAAGTTTTGTGGTATTCAATGGAGCTCTAAAAACATCT
  TCAGGATTTCTTGCTAAGTCCAGCATAGTTGAAGATGGCTTAATGGTACAAATAACTCCAGAGACCATGA
  ATGGCTTGCGGCTAGCTTTACGAGAACAGAAAGACTTTAAAATTACATGTGGGAAAGTTGATGCAGTAGA
  CCTGAGAGAATACGTGGATATCTGCTGGGTAGATGCTGAAGAAAAAGGAAACAAAGGAGTTATCAGTTCA
  GTGGATGGAATATCATTACAAGGATTTCCAAGTGAAAAAATAAAACTGGAAGCAGATTTTGAAACCGATG
  AGAAGATTGTAAAATGTACCGAGGTGTTCTACTTTCTAAAGGACCAGGATTTATCTATTTTATCAACTTC
  TTATCAGTTTGCAAAAGAAATAGCCATGGCTTGTAGTGCTGCGCTGTGCCCTCACCTGAAAACTCTAAAA
  AGTAATGGGATGAATAAAATTGGACTCAGAGTTTCCATTGACACTGATATGGTTGAATTTCAGGCAGGAT
  CTGAAGGCCAACTTCTGCCTCAGCATTATCTAAATGATCTTGATAGTGCTCTGATACCTGTGATCCATGG
  TGGGACCTCCAACTCTAGTTTACCATTAGAAATAGAATTAGTGTTTTTCATTATAGAACATCTTTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226406 representing NM_001105251
  Red=Cloning site Green=Tags(s)
  
  MDSYFKAAVSDLDKLLDDFEQNPDEQDYLQDVQNAYDSNHCSVSSELASSQRTSLLPKDQECVNSCASSE
  TSYGTNESSLNEKTLKGLTSIQNEKNVTGLDLLSSVDGGTSDEIQPLYMGRCSKPICDLISDMGNLVHAT
  NSEEDIKKLLPDDFKSNADSLIGLDLSSVSDTPCVSSTDHDSDTVREQQNDISSELQNREIGGIKELGIK
  VDTTLSDSYNYSGTENLKDKKIFNQLESIVDFNMSSALTRQSSKMFHAKDKLQHKSQPCGLLKDVGLVKE
  EVDVAVITAAECLKEEGKTSALTCSLPKNEDLCLNDSNSRDENFKLPDFSFQEDKTVIKQSAQEDSKSLD
  LKDNDVIQDSSSALHVSSKDVPSSLSCLPASGSMCGSLIESKARGDFLPQHEHKDNIQDAVTIHEEIQNS
  VVLGGEPFKENDLLKQEKCKSILLQSLIEGMEDRKIDPDQTVIRAESLDGGDTSSTVVESQEGLSGTHVP
  ESSDCCEGFINTFSSNDMDGQDLDYFNIDEGAKSGPLISDAELDAFLTEQYLQTTNIKSFEENVNDSKSQ
  MNQIDMKGLDDGNINNIYFNAEAGAIGESHGINIICETVDKQNTIENGLSLGEKSTIPVQQGLPTSKSEI
  TNQLSVSDINSQSVGGARPKQLFSLPSRTRSSKDLNKPDVPDTIESEPSTADTVVPITCAIDSTADPQVS
  FNSNYIDIESNSEGGSSFVTANEDSVPENTCKEGLVLGQKQPTWVPDSEAPNCMNCQVKFTFTKRRHHCR
  ACGKVFCGVCCNRKCKLQYLEKEARVCVVCYETISKAQAFERMMSPTGSNLKSNHSDECTTVQPPQENQT
  SSIPSPATLPVSALKQPGVEGLCSKEQKRVWFADGILPNGEVADTTKLSSGSKRCSEDFSPLSPDVPMTV
  NTVDHSHSTTVEKPNNETGDITRNEIIQSPISQVPSVEKLSMNTGNEGLPTSGSFTLDDDVFAETEEPSS
  PTGVLVNSNLPIASISDYRLLCDINKYVCNKISLLPNDEDSLPPLLVASGEKGSVPVVEEHPSHEQIILL
  LEGEGFHPVTFVLNANLLVNVKFIFYSSDKYWYFSTNGLHGLGQAEIIILLLCLPNEDTIPKDIFRLFIT
  IYKDALKGKYIENLDNITFTESFLSSKDHGGFLFITPTFQKLDDLSLPSNPFLCGILIQKLEIPWAKVFP
  MRLMLRLGAEYKAYPAPLTSIRGRKPLFGEIGHTIMNLLVDLRNYQYTLHNIDQLLIHMEMGKSCIKIPR
  KKYSDVMKVLNSSNEHVISIGASFSTEADSHLVCIQNDGIYETQANSATGHPRKVTGASFVVFNGALKTS
  SGFLAKSSIVEDGLMVQITPETMNGLRLALREQKDFKITCGKVDAVDLREYVDICWVDAEEKGNKGVISS
  VDGISLQGFPSEKIKLEADFETDEKIVKCTEVFYFLKDQDLSILSTSYQFAKEIAMACSAALCPHLKTLK
  SNGMNKIGLRVSIDTDMVEFQAGSEGQLLPQHYLNDLDSALIPVIHGGTSNSSLPLEIELVFFIIEHLF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001105251
• ORF Size: 4617 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001105251.1, NP_001098721.1
• RefSeq Size: 7224
• RefSeq ORF: 4620
• Locus ID: 9765
• Protein Pathways: TGF-beta signaling pathway
• Gene Summary: This gene encodes an endosomal protein that belongs to the FYVE zinc finger family of proteins. The encoded protein is thought to regulate membrane trafficking in the endosome. This protein functions as a scaffold protein in the transforming growth factor-beta signaling pathway and is involved in positive and negative feedback regulation of the bone morphogenetic protein signaling pathway. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]