NFYC (NM_001142588) Human Tagged ORF Clone
CAT#: RG226525
- TrueORF®
NFYC (GFP-tagged) - Human nuclear transcription factor Y, gamma (NFYC), transcript variant 1
"NM_001142588" in other vectors (4)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | NFYC |
Synonyms | CBF-C; CBFC; H1TF2A; HAP5; HSM; NF-YC |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG226525 representing NM_001142588
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCACAGAAGGAGGATTTGGTGGTACTAGCAGCAGTGATGCCCAGCAAAGCCTACAGTCGTTCTGGC CTCGGGTCATGGAAGAAATCCGGAATTTAACAGTGAAAGACTTCCGAGTGCAGGAACTCCCACTGGCTCG TATTAAGAAGATTATGAAACTGGATGAAGATGTGAAGATGATCAGTGCAGAAGCGCCTGTACTCTTTGCC AAGGCAGCCCAGATTTTTATCACAGAGTTGACTCTTCGAGCCTGGATTCACACAGAAGATAACAAGCGCC GGACTCTACAGAGAAATGATATCGCCATGGCAATTACAAAATTTGATCAGTTTGATTTTCTCATCGATAT TGTTCCAAGAGATGAACTGAAACCTCCAAAGCGTCAGGAGGAGGTGCGCCAGTCTGTAACTCCTGCCGAG CCAGTCCAGTACTATTTCACGCTGGCTCAGCAACCCACCGCTGTCCAAGTCCAGGGCCAGCAGCAAGGCC AGCAGACCACCAGCTCCACGACCACCATCCAGCCTGGGCAGATCATCATCGCACAGCCTCAGCAGGGCCA GACCACACCTGTGACAATGCAGGTTGGAGAAGGTCAGCAGGTGCAGATTGTCCAGGCTCAGCCACAGGGT CAAGCCCAACAGGCCCAGAGTGGCACTGGACAGACCATGCAGGTGATGCAGCAGATCATCACTAACACAG GAGAGATCCAGCAGATCCCGGTGCAGCTGAATGCCGGCCAGCTGCAGTATATCCGCTTAGCCCAGCCTGT ATCAGGCACTCAAGTTGTGCAGGGACAGATCCAGACACTTGCCACCAATGCTCAACAGGGGCAAAGAAAT GCAAGTCAGGGGAAGCCTCGAAGGTGCCTGAAAGAAACCTTACAGATTACACAGACAGAGGTCCAGCAAG GACAGCAGCAGTTCAGCCAGTTCACAGATGGACAGCAGCTCTACCAGATCCAGCAAGTCACCATGCCTGC GGGCCAGGACCTCGCCCAGCCCATGTTCATCCAGTCAGCCAACCAGCCCTCCGACGGGCAGGCCCCCCAG GTGACCGGCGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226525 representing NM_001142588
Red=Cloning site Green=Tags(s) MSTEGGFGGTSSSDAQQSLQSFWPRVMEEIRNLTVKDFRVQELPLARIKKIMKLDEDVKMISAEAPVLFA KAAQIFITELTLRAWIHTEDNKRRTLQRNDIAMAITKFDQFDFLIDIVPRDELKPPKRQEEVRQSVTPAE PVQYYFTLAQQPTAVQVQGQQQGQQTTSSTTTIQPGQIIIAQPQQGQTTPVTMQVGEGQQVQIVQAQPQG QAQQAQSGTGQTMQVMQQIITNTGEIQQIPVQLNAGQLQYIRLAQPVSGTQVVQGQIQTLATNAQQGQRN ASQGKPRRCLKETLQITQTEVQQGQQQFSQFTDGQQLYQIQQVTMPAGQDLAQPMFIQSANQPSDGQAPQ VTGD TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001142588 |
ORF Size | 1062 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001142588.1, NP_001136060.1 |
RefSeq Size | 2162 bp |
RefSeq ORF | 1065 bp |
Locus ID | 4802 |
Cytogenetics | 1p34.2 |
Protein Families | Transcription Factors |
Protein Pathways | Antigen processing and presentation |
Gene Summary | 'This gene encodes one subunit of a trimeric complex forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoters of a variety of genes. The encoded protein, subunit C, forms a tight dimer with the B subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC325811 | NFYC (untagged)-Human nuclear transcription factor Y, gamma (NFYC), transcript variant 1 |
USD 600.00 |
|
RC226525 | NFYC (Myc-DDK-tagged)-Human nuclear transcription factor Y, gamma (NFYC), transcript variant 1 |
USD 420.00 |
|
RC226525L3 | Lenti ORF clone of Human nuclear transcription factor Y, gamma (NFYC), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC226525L4 | Lenti ORF clone of Human nuclear transcription factor Y, gamma (NFYC), transcript variant 1, mGFP tagged |
USD 620.00 |
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