DDX4 (NM_001142549) Human Tagged ORF Clone

CAT#: RG226639

DDX4 (GFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (DDX4), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DDX4
• Synonyms: VASA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226639 representing NM_001142549
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGATGAAGATTGGGAAGCAGAAATCAACCCTCATATGTCTTCCTATGTTCCCATATTTGAGAAGG
  ATAGGTATTCTGGAGAAAATGGAGACAATTTTAACAGGACTCCAGCTTCATCATCAGAAATGGATGATGG
  ACCTTCTCGAAGAGATCATTTCATGAAAAGTGGATTTGCCTCTGGGCGGAATTTTGGAAACAGAGATGCT
  GGTGAGTGTAATAAGCGAGATAATACATCCACAATGGGTGGTTTTGGAGTTGGAAAGAGTTTTGGAAACA
  GAGGTTTTTCAAACAGCAGGTTTGAAGATGGTGATAGCTCTGGTTTCTGGAGAGAGTCTAGTAATGACTG
  CGAAGATAATCCAACACGGAACAGAGGGTTTTCCAAGAGAGGCGATAATGACTTAGACCCAGACGAATGT
  ATGCAGCGCACTGGTGGCCTTTTTGGTTCTAGAAGACCAGTATTAAGTGGCACAGGTAATGGTGATACTT
  CTCAAAGCAGAAGTGGCAGTGGAAGTGAACGAGGTGGTTACAAAGGTTTAAATGAAGAAGTAATAACAGG
  CTCTGGAAAGAATTCTTGGAAGTCAGAAGCAGAAGGAGGAGAAAGTAGTGATACTCAAGGACCAAAAGTG
  ACCTACATACCCCCTCCTCCACCTGAGGATGAGGACTCCATCTTTGCACATTATCAGACAGGCATAAACT
  TCGACAAATACGACACTATTCTTGTGGAAGTGTCTGGACATGATGCACCACCAGCAATTCTGACTTTTGA
  AGAAGCTAATCTCTGTCAGACACTGAATAACAACATTGCTAAAGCTGGTTATACTAAGCTTACTCCTGTG
  CAAAAATACAGTATTCCTATCATACTTGCAGGACGAGATTTGATGGCTTGCGCTCAAACAGGGTCTGGGA
  AGACTGCGGCTTTTCTCCTACCAATTTTGGCTCATATGATGCATGATGGAATAACTGCCAGTCGTTTTAA
  AGAGTTGCAGGAACCAGAGTGTATTATTGTAGCACCAACTCGAGAATTGGTCAACAAGATTTATTTGGAA
  GCCAGAAAATTTTCTTTTGGGACTTGTGTAAGAGCTGTTGTTATATATGGGGGAACCCAGCTGGGACATT
  CAATTCGACAAATAGTACAAGGCTGTAATATATTATGTGCTACTCCTGGAAGACTGATGGATATCATAGG
  CAAAGAAAAGATTGGTCTCAAACAGATCAAATACTTAGTTTTGGATGAAGCTGATCGCATGTTGGATATG
  GGTTTTGGTCCAGAAATGAAGAAGTTAATTTCTTGCCCAGGAATGCCATCAAAGGAACAGCGCCAAACCC
  TTATGTTCAGTGCAACTTTTCCAGAGGAAATTCAAAGGTTGGCTGCAGAGTTTTTAAAGTCAAATTATCT
  GTTTGTTGCTGTTGGACAAGTGGGTGGAGCATGTAGAGATGTTCAGCAGACCGTTCTCCAAGTTGGCCAG
  TTCTCAAAAAGAGAGAAGCTCGTTGAAATTCTGCGAAACATAGGGGATGAAAGAACTATGGTCTTTGTTG
  AAACTAAGAAAAAAGCAGATTTTATTGCAACTTTTCTTTGTCAAGAAAAAATATCAACTACAAGTATTCA
  TGGTGATCGGGAACAGAGAGAGCGGGAGCAAGCTCTTGGAGATTTTCGCTTTGGAAAGTGCCCAGTTCTT
  GTTGCTACTTCAGTAGCTGCCAGAGGGCTGGATATTGAAAATGTGCAACATGTTATCAATTTTGATCTTC
  CTTCTACCATTGATGAATATGTTCATCGAATTGGGCGTACTGGTCGTTGTGGGAATACTGGCAGAGCAAT
  TTCCTTTTTTGATCTTGAATCGGATAACCATTTAGCACAGCCTCTAGTAAAAGTATTGACAGATGCTCAA
  CAGGATGTTCCTGCATGGTTGGAAGAAATTGCCTTTAGTACATACATTCCTGGCTTCAGTGGTAGTACAA
  GAGGAAACGTGTTTGCATCAGTTGATACCAGAAAGGGCAAGAGCACTTTGAACACAGCTGGGTTTTCTTC
  TTCACAAGCTCCCAATCCAGTAGATGATGAGTCATGGGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226639 representing NM_001142549
  Red=Cloning site Green=Tags(s)
  
  MGDEDWEAEINPHMSSYVPIFEKDRYSGENGDNFNRTPASSSEMDDGPSRRDHFMKSGFASGRNFGNRDA
  GECNKRDNTSTMGGFGVGKSFGNRGFSNSRFEDGDSSGFWRESSNDCEDNPTRNRGFSKRGDNDLDPDEC
  MQRTGGLFGSRRPVLSGTGNGDTSQSRSGSGSERGGYKGLNEEVITGSGKNSWKSEAEGGESSDTQGPKV
  TYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPV
  QKYSIPIILAGRDLMACAQTGSGKTAAFLLPILAHMMHDGITASRFKELQEPECIIVAPTRELVNKIYLE
  ARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLKQIKYLVLDEADRMLDM
  GFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAAEFLKSNYLFVAVGQVGGACRDVQQTVLQVGQ
  FSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRFGKCPVL
  VATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDNHLAQPLVKVLTDAQ
  QDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDTRKGKSTLNTAGFSSSQAPNPVDDESWD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142549
• ORF Size: 2070 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001142549.1, NP_001136021.1
• RefSeq Size: 2778
• RefSeq ORF: 2073
• Locus ID: 54514
• Gene Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]