DDX4 (NM_001142549) Human Tagged ORF Clone
CAT#: RG226639
- TrueORF®
DDX4 (GFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (DDX4), transcript variant 2
"NM_001142549" in other vectors (4)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | DDX4 |
Synonyms | VASA |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG226639 representing NM_001142549
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGATGAAGATTGGGAAGCAGAAATCAACCCTCATATGTCTTCCTATGTTCCCATATTTGAGAAGG ATAGGTATTCTGGAGAAAATGGAGACAATTTTAACAGGACTCCAGCTTCATCATCAGAAATGGATGATGG ACCTTCTCGAAGAGATCATTTCATGAAAAGTGGATTTGCCTCTGGGCGGAATTTTGGAAACAGAGATGCT GGTGAGTGTAATAAGCGAGATAATACATCCACAATGGGTGGTTTTGGAGTTGGAAAGAGTTTTGGAAACA GAGGTTTTTCAAACAGCAGGTTTGAAGATGGTGATAGCTCTGGTTTCTGGAGAGAGTCTAGTAATGACTG CGAAGATAATCCAACACGGAACAGAGGGTTTTCCAAGAGAGGCGATAATGACTTAGACCCAGACGAATGT ATGCAGCGCACTGGTGGCCTTTTTGGTTCTAGAAGACCAGTATTAAGTGGCACAGGTAATGGTGATACTT CTCAAAGCAGAAGTGGCAGTGGAAGTGAACGAGGTGGTTACAAAGGTTTAAATGAAGAAGTAATAACAGG CTCTGGAAAGAATTCTTGGAAGTCAGAAGCAGAAGGAGGAGAAAGTAGTGATACTCAAGGACCAAAAGTG ACCTACATACCCCCTCCTCCACCTGAGGATGAGGACTCCATCTTTGCACATTATCAGACAGGCATAAACT TCGACAAATACGACACTATTCTTGTGGAAGTGTCTGGACATGATGCACCACCAGCAATTCTGACTTTTGA AGAAGCTAATCTCTGTCAGACACTGAATAACAACATTGCTAAAGCTGGTTATACTAAGCTTACTCCTGTG CAAAAATACAGTATTCCTATCATACTTGCAGGACGAGATTTGATGGCTTGCGCTCAAACAGGGTCTGGGA AGACTGCGGCTTTTCTCCTACCAATTTTGGCTCATATGATGCATGATGGAATAACTGCCAGTCGTTTTAA AGAGTTGCAGGAACCAGAGTGTATTATTGTAGCACCAACTCGAGAATTGGTCAACAAGATTTATTTGGAA GCCAGAAAATTTTCTTTTGGGACTTGTGTAAGAGCTGTTGTTATATATGGGGGAACCCAGCTGGGACATT CAATTCGACAAATAGTACAAGGCTGTAATATATTATGTGCTACTCCTGGAAGACTGATGGATATCATAGG CAAAGAAAAGATTGGTCTCAAACAGATCAAATACTTAGTTTTGGATGAAGCTGATCGCATGTTGGATATG GGTTTTGGTCCAGAAATGAAGAAGTTAATTTCTTGCCCAGGAATGCCATCAAAGGAACAGCGCCAAACCC TTATGTTCAGTGCAACTTTTCCAGAGGAAATTCAAAGGTTGGCTGCAGAGTTTTTAAAGTCAAATTATCT GTTTGTTGCTGTTGGACAAGTGGGTGGAGCATGTAGAGATGTTCAGCAGACCGTTCTCCAAGTTGGCCAG TTCTCAAAAAGAGAGAAGCTCGTTGAAATTCTGCGAAACATAGGGGATGAAAGAACTATGGTCTTTGTTG AAACTAAGAAAAAAGCAGATTTTATTGCAACTTTTCTTTGTCAAGAAAAAATATCAACTACAAGTATTCA TGGTGATCGGGAACAGAGAGAGCGGGAGCAAGCTCTTGGAGATTTTCGCTTTGGAAAGTGCCCAGTTCTT GTTGCTACTTCAGTAGCTGCCAGAGGGCTGGATATTGAAAATGTGCAACATGTTATCAATTTTGATCTTC CTTCTACCATTGATGAATATGTTCATCGAATTGGGCGTACTGGTCGTTGTGGGAATACTGGCAGAGCAAT TTCCTTTTTTGATCTTGAATCGGATAACCATTTAGCACAGCCTCTAGTAAAAGTATTGACAGATGCTCAA CAGGATGTTCCTGCATGGTTGGAAGAAATTGCCTTTAGTACATACATTCCTGGCTTCAGTGGTAGTACAA GAGGAAACGTGTTTGCATCAGTTGATACCAGAAAGGGCAAGAGCACTTTGAACACAGCTGGGTTTTCTTC TTCACAAGCTCCCAATCCAGTAGATGATGAGTCATGGGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226639 representing NM_001142549
Red=Cloning site Green=Tags(s) MGDEDWEAEINPHMSSYVPIFEKDRYSGENGDNFNRTPASSSEMDDGPSRRDHFMKSGFASGRNFGNRDA GECNKRDNTSTMGGFGVGKSFGNRGFSNSRFEDGDSSGFWRESSNDCEDNPTRNRGFSKRGDNDLDPDEC MQRTGGLFGSRRPVLSGTGNGDTSQSRSGSGSERGGYKGLNEEVITGSGKNSWKSEAEGGESSDTQGPKV TYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPV QKYSIPIILAGRDLMACAQTGSGKTAAFLLPILAHMMHDGITASRFKELQEPECIIVAPTRELVNKIYLE ARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLKQIKYLVLDEADRMLDM GFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAAEFLKSNYLFVAVGQVGGACRDVQQTVLQVGQ FSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRFGKCPVL VATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDNHLAQPLVKVLTDAQ QDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDTRKGKSTLNTAGFSSSQAPNPVDDESWD TRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001142549 |
ORF Size | 2070 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Reference Data | |
RefSeq | NM_001142549.1, NP_001136021.1 |
RefSeq Size | 2778 |
RefSeq ORF | 2073 |
Locus ID | 54514 |
Gene Summary | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC325281 | DDX4 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (DDX4), transcript variant 2 |
USD 760.00 |
|
RC226639 | DDX4 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (DDX4), transcript variant 2 |
USD 420.00 |
|
RC226639L3 | Lenti-ORF clone of DDX4 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (DDX4), transcript variant 2 |
USD 620.00 |
|
RC226639L4 | Lenti-ORF clone of DDX4 (mGFP-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (DDX4), transcript variant 2 |
USD 620.00 |
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