MGEA5 (NM_001142434) Human Tagged ORF Clone

CAT#: RG226720

MGEA5 (GFP-tagged) - Human meningioma expressed antigen 5 (hyaluronidase) (MGEA5), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MGEA5
• Synonyms: MEA5; MGEA5; NCOAT
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226720 representing NM_001142434
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCAGAAGGAGAGTCAAGCGACGTTGGAGGAGCGGGAGAGCGAGCTCAGCTCCAACCCTGCCGCCT
  CTGCGGGGGCATCGCTGGAGCCGCCGGCAGCTCCGGCACCCGGAGAAGACAACCCCGCCGGGGCTGGGGG
  AGCGGCGGTGGCCGGGGCTGCAGGAGGGGCTCGGCGGTTCCTCTGCGGTGTGGTGGAAGGATTTTATGGA
  AGACCTTGGGTTATGGAACAGAGAAAAGAACTCTTTAGAAGGCTCCAGAAATGGGAATTAAATACATACT
  TGTATGCCCCAAAAGATGACTACAAACATAGGATGTTTTGGCGAGAGATGTATTCAGTGGAGGAAGCTGA
  GCAACTTATGACTCTCATCTCTGCTGCACGAGAATATGAGATAGAGTTCATCTATGCGATCTCACCTGGA
  TTGGATATCACTTTTTCTAACCCCAAGGAAGTATCCACATTGAAACGTAAATTGGACCAGGTTTCTCAGT
  TTGGGTGCAGATCATTTGCTTTGCTTTTTGATGATATAGACCATAATATGTGTGCAGCAGACAAAGAGGT
  ATTCAGTTCTTTTGCTCATGCCCAAGTCTCCATCACAAATGAAATCTATCAGTACCTAGGAGAGCCAGAA
  ACTTTCCTCTTCTGTCCCACAGAATACTGTGGCACTTTCTGTTATCCAAATGTGTCTCAGTCTCCATATT
  TAAGGACTGTGGGTGAAAAGCTTCTACCTGGAATTGAAGTGCTTTGGACAGGTCCCAAAGTTGTTTCTAA
  AGAAATTCCAGTAGAGTCCATCGAAGAGGTTTCTAAGATTATTAAGAGAGCTCCAGTAATCTGGGATAAC
  ATTCATGCTAATGATTATGATCAGAAGAGACTGTTTCTGGGCCCGTACAAAGGAAGATCCACAGAACTCA
  TCCCACGGTTAAAAGGAGTCCTCACTAATCCAAATTGTGAATTTGAAGCCAACTACGTTGCTATCCACAC
  CCTTGCCACCTGGTACAAATCAAACATGAATGGAGTGAGAAAAGATGTAGTGATGAGTAGGCAAGTTGCA
  CACAGTGGAGCTAAAGCAAGTGTAGTTGATGGGACTCCTTTAGTTGCAGCACCCTCTTTAAATGCCACAA
  CCGTAGTAACAACAGTTTATCAGGAGCCCATTATGAGCCAGGGAGCAGCCTTGAGTGGTGAGCCTACTAC
  TCTGACCAAGGAAGAAGAAAAGAAACAGCCTGATGAAGAACCCATGGACATGGTGGTGGAAAAACAAGAA
  GAAACGGACCACAAGAATGACAATCAAATACTGAGTGAAATTGTTGAAGCGAAAATGGCAGAGGAATTGA
  AACCAATGGACACTGATAAAGAGAGCATAGCTGAATCAAAATCCCCAGAGATGTCCATGCAAGAAGATTG
  TATTAGTGACATTGCCCCCATGCAAACTGATGAACAGACAAACAAGGAGCAGTTTGTGCCAGGTCCAAAT
  GAAAAGCCTTTGTACACTGCGGAACCAGTGACCCTGGAGGATTTGCAGTTACTTGCTGATCTATTCTACC
  TTCCTTACGAGCATGGACCCAAAGGAGCACAGATGTTACGGGAATTTCAATGGCTTCGAGCAAATAGTAG
  TGTTGTCAGTGTCAATTGCAAAGGAAAAGACTCTGAAAAAATTGAAGAATGGCGGTCACGAGCAGCCAAG
  TTTGAAGAGATGTGTGGACTAGTGATGGGAATGTTCACTCGGCTCTCCAATTGTGCCAACAGGACAATTC
  TTTATGACATGTACTCCTATGTTTGGGATATCAAGAGTATAATGTCTATGGTGAAGTCTTTTGTACAGTG
  GTTAGGGTGTCGTAGTCATTCTTCAGCACAATTCTTAATTGGAGACCAAGAACCCTGGGCCTTTAGAGGT
  GGTCTAGCAGGAGAGTTCCAGCGTTTGCTGCCAATTGATGGGGCAAATGATCTCTTTTTTCAGCCACCTC
  CACTGACTCCTACCTCCAAAGTTTATACTATCAGACCTTATTTTCCTAAGGATGAGGCATCCGTGTACAA
  GATTTGCAGAGAAATGTATGACGATGGAGTGGGTTTACCCTTTCAAAGTCAGCCTGATCTTATTGGAGAC
  AAGTTAGTAGGAGGGCTGCTTTCCCTCAGCCTGGATTACTGCTTTGTCCTAGAAGATGAAGATGGCATAT
  GTGGTTATGCCTTGGGCACTGTAGATGTGACCCCCTTTATTAAAAAATGTAAAATTTCCTGGATCCCCTT
  CATGCAGGAGAAGTATACCAAGCCAAATGGTGACAAGGAACTCTCTGAGGCTGAGAAAATAATGTTGAGT
  TTCCATGAAGAACAGGAAGTACTGCCAGAAACTTTCCTTGCTAATTTCCCTTCTCTGATAAAGATGGACA
  TTCACAAAAAAGTAACTGACCCAAGTGTGGCCAAAAGCATGATGGCTTGCCTCCTGTCTTCACTGAAGGC
  TAATGGCTCCCGGGGAGCTTTCTGTGAAGTGAGACCAGATGATAAAAGAATTCTGGAATTTTACAGCAAG
  TTAGGATGTTTTGAAATTGCAAAAATGGAAGGATTTCCAAAGGATGTGGTTATACTTGGTCGGAGCCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226720 representing NM_001142434
  Red=Cloning site Green=Tags(s)
  
  MVQKESQATLEERESELSSNPAASAGASLEPPAAPAPGEDNPAGAGGAAVAGAAGGARRFLCGVVEGFYG
  RPWVMEQRKELFRRLQKWELNTYLYAPKDDYKHRMFWREMYSVEEAEQLMTLISAAREYEIEFIYAISPG
  LDITFSNPKEVSTLKRKLDQVSQFGCRSFALLFDDIDHNMCAADKEVFSSFAHAQVSITNEIYQYLGEPE
  TFLFCPTEYCGTFCYPNVSQSPYLRTVGEKLLPGIEVLWTGPKVVSKEIPVESIEEVSKIIKRAPVIWDN
  IHANDYDQKRLFLGPYKGRSTELIPRLKGVLTNPNCEFEANYVAIHTLATWYKSNMNGVRKDVVMSRQVA
  HSGAKASVVDGTPLVAAPSLNATTVVTTVYQEPIMSQGAALSGEPTTLTKEEEKKQPDEEPMDMVVEKQE
  ETDHKNDNQILSEIVEAKMAEELKPMDTDKESIAESKSPEMSMQEDCISDIAPMQTDEQTNKEQFVPGPN
  EKPLYTAEPVTLEDLQLLADLFYLPYEHGPKGAQMLREFQWLRANSSVVSVNCKGKDSEKIEEWRSRAAK
  FEEMCGLVMGMFTRLSNCANRTILYDMYSYVWDIKSIMSMVKSFVQWLGCRSHSSAQFLIGDQEPWAFRG
  GLAGEFQRLLPIDGANDLFFQPPPLTPTSKVYTIRPYFPKDEASVYKICREMYDDGVGLPFQSQPDLIGD
  KLVGGLLSLSLDYCFVLEDEDGICGYALGTVDVTPFIKKCKISWIPFMQEKYTKPNGDKELSEAEKIMLS
  FHEEQEVLPETFLANFPSLIKMDIHKKVTDPSVAKSMMACLLSSLKANGSRGAFCEVRPDDKRILEFYSK
  LGCFEIAKMEGFPKDVVILGRSL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142434
• ORF Size: 2589 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001142434.1, NP_001135906.1
• RefSeq Size: 5061
• RefSeq ORF: 2592
• Locus ID: 10724
• Gene Summary: The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]). [supplied by OMIM, Mar 2008]